BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,786,541	C→T	3.6%	A225A (GCC→GCT)	RCAP_rcc01648 →	ABC transporter permease

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,786,541	0	C→T	3.6%	10.0 ‑1.4 ‑0.3	192	A225A (GCC→GCT)	RCAP_rcc01648	ABC transporter permease
Reads supporting (aligned to +/- strand): new base (1/6): ref base (81/104): total (82/110)
Fisher's exact test strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.50e-01

Only 100 of 203 total aligned reads displayed.


TGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GGCCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATCC  >  NC_014034/1786444‑1786641


 gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGGCTttc                                                                                                   <  1:2713295/101‑2 (MQ=255)


  aTGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCg                                                                                                 <  1:326077/101‑1 (MQ=255)


  aTGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCg                                                                                                 >  1:3034600/1‑101 (MQ=255)


  aTGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCg                                                                                                 <  2:2412961/101‑1 (MQ=255)


   tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGt                                                                                                <  1:761709/101‑1 (MQ=255)


     ccGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTAt                                                                                              <  1:3576420/101‑1 (MQ=255)


     ccGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTAt                                                                                              <  1:1712299/101‑1 (MQ=255)


     ccGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGAT.GGGCTTTCGTAt                                                                                              <  2:1889941/101‑1 (MQ=255)


      cGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATc                                                                                             <  1:507803/101‑1 (MQ=255)


         aTCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCAt                                                                                          >  2:2447281/1‑101 (MQ=255)


            tGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTg                                                                                       <  1:1181163/101‑1 (MQ=255)


            tGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTg                                                                                       <  2:643438/101‑1 (MQ=255)


             gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                      >  2:2492948/1‑101 (MQ=255)


             gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                      <  1:1534727/101‑1 (MQ=255)


              aTCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGaa                                                                                     >  2:4383030/1‑101 (MQ=255)


               tCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGact                                                                                    >  2:758390/1‑99 (MQ=255)


               tCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAt                                                                                    <  1:3219036/101‑1 (MQ=255)


                 gccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAttt                                                                                  <  2:1562582/101‑1 (MQ=255)


                 gccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAttt                                                                                  <  1:1449902/101‑1 (MQ=255)


                 gccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGAT.GGGCTTTCGTATCCATCTGAAttt                                                                                  >  2:3701625/1‑101 (MQ=255)


                   cgcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                                >  1:2863114/1‑101 (MQ=255)


                      ggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGa                                                                             <  2:574600/101‑1 (MQ=255)


                       gcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGAt                                                                            <  2:1235892/101‑1 (MQ=255)


                          cgggcgggGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGtt                                                                         <  2:4196906/101‑1 (MQ=255)


                           gggcggggCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                        >  2:2299256/1‑101 (MQ=255)


                            ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCTAACGGGATCGG.CTTTCGTTTCCATCTGAATTTCCAGATGTTCg                                                                       <  1:112826/101‑1 (MQ=255)


                            ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                       <  2:1166927/101‑1 (MQ=255)


                              cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                     <  1:2918364/95‑1 (MQ=255)


                               ggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACa                                                                    <  1:1766898/96‑1 (MQ=255)


                               ggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACa                                                                    <  1:2059074/96‑1 (MQ=255)


                                gggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAt                                                                   >  1:1368559/5‑101 (MQ=255)


                                 ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                                  <  1:2986344/98‑1 (MQ=255)


                                   ctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                                >  2:1743553/2‑101 (MQ=255)


                                   ctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                                <  1:20638/100‑1 (MQ=255)


                                    tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGcc                                                               >  2:3443198/1‑101 (MQ=255)


                                      tCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGa                                                             <  2:541512/101‑1 (MQ=255)


                                      tCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGa                                                             >  2:878963/1‑101 (MQ=255)


                                      tCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTCCGACATTGCCGa                                                             <  1:2429297/101‑1 (MQ=255)


                                        gCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAgg                                                           <  2:347063/101‑1 (MQ=255)


                                         cTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGt                                                          >  2:2379160/1‑101 (MQ=255)


                                         cTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGt                                                          <  2:204663/101‑1 (MQ=255)


                                         cTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGt                                                          >  1:235015/1‑101 (MQ=255)


                                          tGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTg                                                         >  2:3290579/1‑101 (MQ=255)


                                          tGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTg                                                         >  2:147809/1‑101 (MQ=255)


                                           gATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGc                                                        >  1:3706398/1‑101 (MQ=255)


                                           gATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGACGAGGTGc                                                        >  2:612033/1‑101 (MQ=255)


                                            aTCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCt                                                       >  1:2316229/1‑101 (MQ=255)


                                             tCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTc                                                      <  2:3782301/101‑1 (MQ=255)


                                              cTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCg                                                     >  2:2572809/1‑101 (MQ=255)


                                                  aaGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTg                                                 <  2:1218740/101‑1 (MQ=255)


                                                   aGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTgg                                                <  2:786058/101‑1 (MQ=255)


                                                   aGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTgg                                                <  1:2030972/101‑1 (MQ=255)


                                                    ggTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTggg                                               <  1:4383302/101‑1 (MQ=255)


                                                     gTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggc                                              >  1:1654345/1‑101 (MQ=255)


                                                     gTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggc                                              >  2:3036154/1‑101 (MQ=255)


                                                        gTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                           >  2:3820631/1‑101 (MQ=255)


                                                        gTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                           >  1:3332311/1‑101 (MQ=255)


                                                         tGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcg                                          <  1:1984407/101‑1 (MQ=255)


                                                           cTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcggc                                        <  2:1500721/101‑1 (MQ=255)


                                                                ggtCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCg                                   <  1:2507197/101‑1 (MQ=255)


                                                                 gtCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGt                                  >  2:1542655/1‑101 (MQ=255)


                                                                   cGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCt                                >  2:4066044/1‑101 (MQ=255)


                                                                     aaTTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGc                              <  2:4249625/101‑1 (MQ=255)


                                                                      aTTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCAGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCg                             >  2:2823916/1‑101 (MQ=255)


                                                                        ttctgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTg                           <  2:4418705/96‑1 (MQ=255)


                                                                         tctgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGa                          >  1:3089563/5‑101 (MQ=255)


                                                                           tgggCCGCTCGAACGGGATTGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCGGGGCGGCGGCTTTCGTCTGCGTGATg                        <  1:2379310/99‑1 (MQ=255)


                                                                           tgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                        >  2:4320900/3‑101 (MQ=255)


                                                                           tgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                        >  1:1034408/3‑101 (MQ=255)


                                                                           tgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                        <  2:234988/99‑1 (MQ=255)


                                                                             ggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCt                      <  1:852674/101‑1 (MQ=255)


                                                                               ccGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCg                    <  2:3387089/101‑1 (MQ=255)


                                                                                cGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGCCTGCGGGATGCTCGc                   >  2:3064035/1‑101 (MQ=255)


                                                                                 gCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGcc                  <  2:1987287/101‑1 (MQ=255)


                                                                                  cTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCg                 <  2:680697/101‑1 (MQ=255)


                                                                                  cTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCg                 <  2:1439105/101‑1 (MQ=255)


                                                                                   tCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGt                <  2:326165/101‑1 (MQ=255)


                                                                                    cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc               <  1:582609/101‑1 (MQ=255)


                                                                                     gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg              <  1:879048/101‑1 (MQ=255)


                                                                                     gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg              >  2:2923103/1‑101 (MQ=255)


                                                                                     gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGACGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg              >  1:3801945/1‑101 (MQ=255)


                                                                                      aaCGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGa             >  1:2577394/1‑101 (MQ=255)


                                                                                      aaCGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGa             <  1:3658691/101‑1 (MQ=255)


                                                                                       aCGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGaa            >  2:161337/1‑101 (MQ=255)


                                                                                       aCGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGaa            >  1:724089/1‑101 (MQ=255)


                                                                                       aCGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGaa            >  1:1923109/1‑101 (MQ=255)


                                                                                        cGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAAt           <  1:3443437/101‑1 (MQ=255)


                                                                                        cGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAAt           <  2:1061516/101‑1 (MQ=255)


                                                                                        cGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGGCGAAt           >  2:500143/1‑101 (MQ=255)


                                                                                         gggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATg          >  1:645940/1‑101 (MQ=255)


                                                                                           gATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGc        >  2:3173172/1‑101 (MQ=255)


                                                                                           gATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGc        >  1:1530207/1‑101 (MQ=255)


                                                                                            aTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCt       >  1:3960083/1‑101 (MQ=255)


                                                                                            aTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCt       >  2:1262286/1‑101 (MQ=255)


                                                                                              tcggcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTg      <  2:3954804/97‑1 (MQ=255)


                                                                                               cggcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGa     >  1:2794379/4‑101 (MQ=255)


                                                                                               cggcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGa     <  2:2862906/98‑1 (MQ=255)


                                                                                                 gcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATc   >  1:3903328/2‑101 (MQ=255)


                                                                                                 gcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATc   >  1:3605566/2‑101 (MQ=255)


                                                                                                  cTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATcc  >  2:2715678/1‑101 (MQ=255)


TGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GGCCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATCC  >  NC_014034/1786444‑1786641

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG