Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,813,103 G→C 100% intergenic (+147/‑71) glnA3 → / → fabD glutamine synthetase/ACP S‑malonyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,813,1030G→C100.0% 255.7 76intergenic (+147/‑71)glnA3/fabDglutamine synthetase/ACP S‑malonyltransferase
Reads supporting (aligned to +/- strand):  new base (71/5):  ref base (0/0):  total (71/5)

GGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCTCGGGGGTCCGGCGGCGCGAA.CGCCCGGCG.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTC  >  NC_014034/1813010‑1813194
                                                                                              |                                                                                            
ggTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggc                                                                                        >  2:3830251/1‑101 (MQ=37)
   gcgactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgct                                                                                     >  1:470921/1‑99 (MQ=37)
   gcgactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgct                                                                                     >  2:884978/1‑99 (MQ=37)
     gcctgcgtctTTTTGCTTTTTGGAGCAAAGAAAAACCCCGCCGCTTTTTCTTGGTTAAAATCCGCCGGGGGGGTCCGGGGGCGCAAA.CCCCCCGGC.GCTTc                                                                                 <  2:2160239/99‑1 (MQ=255)
     gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                                   >  1:1318923/1‑97 (MQ=25)
     gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGAGAAGCCCCcggcgcttc                                                                                   >  2:1189615/1‑97 (MQ=25)
             ctctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCt                                                                         >  2:3226165/1‑101 (MQ=25)
               ctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTcc                                                                       >  2:2352628/1‑101 (MQ=25)
                tttGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCg                                                                      >  2:2333250/1‑101 (MQ=25)
                tttGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCg                                                                      >  2:2826118/1‑101 (MQ=25)
                tttGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCg                                                                      >  1:24833/1‑101 (MQ=25)
                  tGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAA..GCCCCCCGCGCTTCCGGTTGCTCCGGa                                                                    >  1:636008/1‑101 (MQ=25)
                   gCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGAc                                                                   >  1:1214831/1‑101 (MQ=25)
                   gCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGAc                                                                   >  1:291118/1‑101 (MQ=25)
                    cGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACg                                                                  >  2:1631421/1‑101 (MQ=25)
                     gTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGc                                                                 >  1:1454895/1‑101 (MQ=25)
                     gTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGc                                                                 >  2:2593081/1‑101 (MQ=25)
                     gTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGc                                                                 >  1:2285762/1‑101 (MQ=25)
                     gTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGc                                                                 >  2:4341453/1‑101 (MQ=25)
                     gTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGc                                                                 >  2:395796/1‑101 (MQ=25)
                     gTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGc                                                                 >  1:738844/1‑101 (MQ=25)
                      tATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCt                                                                >  2:2352441/1‑101 (MQ=25)
                       aTTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTg                                                               >  2:3392812/1‑101 (MQ=25)
                         ttGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCg                                                             >  2:2730863/1‑101 (MQ=37)
                         ttGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCg                                                             >  1:3857256/1‑101 (MQ=37)
                          tGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGt                                                            >  1:2586995/1‑101 (MQ=37)
                          tGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGt                                                            >  2:1564013/1‑101 (MQ=25)
                              ccAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAg                                                        >  1:2210668/1‑101 (MQ=37)
                                aaGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGtt                                                      >  1:465805/1‑101 (MQ=25)
                                 aGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTa                                                     >  1:2819127/1‑101 (MQ=37)
                                       aaCCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGCACGCTGCGTCAAGTTAGAGAAg                                               >  1:3346635/1‑101 (MQ=25)
                                        aCCCGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAgg                                              >  2:2544935/1‑101 (MQ=25)
                                         cccGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGc                                             >  1:4250445/1‑101 (MQ=25)
                                          ccgggtttttttCTTGGTTCAAATGCGCCGGGGGGGGGCGGGGGCGGGAAGCCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGc                                             <  1:1298758/94‑1 (MQ=255)
                                          ccGGCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGcc                                            >  1:1046997/1‑101 (MQ=25)
                                            ggCGCTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAg                                          >  2:165833/1‑101 (MQ=25)
                                              cgcTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGAt                                        >  1:422448/1‑101 (MQ=25)
                                               gcTTTTTCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGAtt                                       >  1:2060259/1‑101 (MQ=25)
                                                  ttttCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGt                                    >  2:884383/1‑101 (MQ=25)
                                                  ttttCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGt                                    >  2:3342145/1‑101 (MQ=25)
                                                   tttCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTc                                   >  1:1917791/1‑101 (MQ=25)
                                                   tttCTTGGTTCAAAGACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTc                                   <  2:1318826/101‑1 (MQ=25)
                                                    ttCTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCa                                  >  1:2247119/1‑101 (MQ=25)
                                                      cTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                                >  2:484957/1‑101 (MQ=37)
                                                      cTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                                >  1:1369412/1‑101 (MQ=25)
                                                      cTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                                >  2:4277872/1‑101 (MQ=25)
                                                      cTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                                >  1:487539/1‑101 (MQ=25)
                                                      cTTGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                                >  1:1274168/1‑101 (MQ=25)
                                                        tGGTTCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagga                              >  2:947688/1‑101 (MQ=25)
                                                            tCAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCg                          >  2:2241021/1‑101 (MQ=25)
                                                             cAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCgg                         >  1:878374/1‑101 (MQ=25)
                                                              aaaTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCggg                        >  1:4108246/1‑101 (MQ=37)
                                                              aaaTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCggg                        >  1:796273/1‑101 (MQ=37)
                                                               aaTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGc                       >  1:1604789/1‑101 (MQ=37)
                                                               aaTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGc                       >  2:3811970/1‑101 (MQ=37)
                                                                aTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCa                      >  2:1943657/1‑101 (MQ=37)
                                                                aTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCa                      >  1:4320733/1‑101 (MQ=37)
                                                                 tACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCAt                     >  1:1549352/1‑101 (MQ=37)
                                                                 tACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCAt                     >  2:1223544/1‑101 (MQ=37)
                                                                 gaggccgggggggCCCGGGGGGGCGAA.CCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCAt                     <  1:1972055/93‑1 (MQ=255)
                                                                   cGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGa                   >  1:2495044/1‑101 (MQ=37)
                                                                   cGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGa                   >  2:3391402/1‑101 (MQ=37)
                                                                    gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAg                  >  2:1743590/1‑101 (MQ=37)
                                                                       gcGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGt               >  1:2794536/2‑101 (MQ=37)
                                                                       gcGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGt               >  2:3114575/2‑101 (MQ=37)
                                                                        cGGGGGTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTg              >  1:3283440/1‑101 (MQ=38)
                                                                         gggggTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg             >  2:2172173/1‑101 (MQ=38)
                                                                         gggggTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg             >  1:3538298/1‑101 (MQ=38)
                                                                         gggggTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg             >  1:3017141/1‑101 (MQ=38)
                                                                         gggggTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg             >  2:3937158/1‑101 (MQ=38)
                                                                          ggggTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGc            >  2:232726/1‑101 (MQ=38)
                                                                          ggggTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGc            >  1:924127/1‑101 (MQ=38)
                                                                          ggggTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGc            >  2:2558770/1‑101 (MQ=38)
                                                                           gggTCCGGGGGCGGGAA.CCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCa           <  2:2117941/101‑1 (MQ=255)
                                                                             gTCCGGGGGCGCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATg         >  2:4182745/1‑101 (MQ=38)
                                                                                ggggggcgCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGgt      <  1:2119156/97‑1 (MQ=255)
                                                                                 gggggcgCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGgtg     >  1:750563/4‑101 (MQ=255)
                                                                                 gggggcgCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGgtg     >  1:924079/4‑101 (MQ=255)
                                                                                   gggcgCGAA.CCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGtt   <  2:3609471/100‑1 (MQ=255)
                                                                                    ggcgCGAAGCCCCCGGC..GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTc  >  2:701471/1‑101 (MQ=255)
                                                                                              |                                                                                            
GGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTTCAAATACGCCTCGGGGGTCCGGCGGCGCGAA.CGCCCGGCG.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTTC  >  NC_014034/1813010‑1813194

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG