Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,951,208 G→C 88.4% A129G (GCG→GGG)  ssb1 ← single‑stranded DNA‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,951,2080G→C88.4% 34.3 ‑0.2 ‑0.0129A129G (GCG→GGG) ssb1single‑stranded DNA‑binding protein
Reads supporting (aligned to +/- strand):  new base (63/51):  ref base (9/6):  total (72/57)
Fisher's exact test strand distribution p-value = 7.88e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.95e-01

Only 100 of 135 total aligned reads displayed.

CCCGACGAGGGGGCC.GAGGAGCCGCCGCCACCGCCGCCATAGCCCGCGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCGCG.C.GTAATTGCCACCGCCGCGCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCG  >  NC_014034/1951115‑1951298
                                                                                               |                                                                                            
cccgcgggggggcCC.GAGGAGCCCCCGCCCCCGCCGCCATGGCCCGGGCCTT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAAtt                                                                                     <  1:4152986/93‑1 (MQ=255)
cccGCCGGGGGGCCG.GGGGACCCCCCGCCCCCCCCGCCATAGCCCGGGCCGT.CATCGTAGCCCCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAAtt                                                                                     <  1:3389424/101‑1 (MQ=255)
cccGACGAGGGGGCC.GAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAAtt                                                                                     >  2:66234/1‑101 (MQ=255)
 ccGACGGGGGGGCG.GAGGACCCGCCGCCCCCGCCGCCATAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTg                                                                                    <  2:3331976/101‑1 (MQ=255)
   gacgaGGGGGCC.GAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGcc                                                                                  >  2:1932703/1‑101 (MQ=255)
   gacgaGGAGGCC.GAGGAGCCGCCGCCACCGCCGCCATAGCCCAGGCCGT.CATCGTAGCCGCCGACATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGcc                                                                                  >  2:2365033/1‑101 (MQ=255)
     ggaGGGGGCC.GGGGAGCCGCCGCCCCCGCCGCCATAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCAc                                                                                <  1:1409669/100‑1 (MQ=255)
      gaGGGGGCC.GAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCAcc                                                                               >  1:3094793/1‑101 (MQ=255)
       ggggggCCGGGGGAGCCCCCGCCCCCGCCGCCATAGCCCGGGCGGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCAcc                                                                               <  2:2314022/100‑1 (MQ=255)
       ggggggCCGGGGGACCCGCCGCCCCCGCCGCCATAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGC.CGCC.GTAATTGCCAcc                                                                               <  1:4353449/100‑1 (MQ=255)
       ggggggCC.GAGGGGCCGCCGCCCCCGCCGCCATAGCCCGGGCCCT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCAccg                                                                              <  1:1284068/100‑1 (MQ=255)
       aGGGGGCC.GAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCAccg                                                                              >  1:1823812/1‑101 (MQ=255)
         ggggCC.GAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCAccgcc                                                                            >  2:632118/1‑101 (MQ=255)
          ggccc.GGGGACCCGCCCCCCCCGCCGCCATAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGC.CGCC.GTAATTGCCAccgccg                                                                           <  1:1238270/98‑1 (MQ=255)
            gCC.GGGGACCCGCCGCCCCCGCCGCCATAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACcgccgcc                                                                         <  2:3178635/101‑2 (MQ=255)
            gCC.GCGGCGCCCCCCCCGCCCCCGCCATAGCCCGGGCGGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACcgccgcc                                                                         <  2:1853198/101‑2 (MQ=255)
            gCC.GAGGAGCCGCCGCCACCGCCGCCATAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGC.CGCC.GTAATTGCCACcgccgcc                                                                         <  2:2990569/101‑2 (MQ=255)
                gaggagCCGCCGCCACCGCCGCCATAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGa                                                                      >  1:3550342/1‑101 (MQ=255)
                 gggcccccccgcccccgccgccaTAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAg                                                                     <  2:3032558/93‑1 (MQ=255)
                 gggacCCCCCCCCCCCGCCGCCATAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAg                                                                     <  1:1148257/100‑1 (MQ=255)
                  ngagCCGCCGCCACCGCCGCCATAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGNNCNAgn                                                                    >  1:1031186/2‑100 (MQ=255)
                  ggacCCCCCCCCCCCGCCGCCATAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAgc                                                                    <  1:3124794/101‑1 (MQ=255)
                     cccgccccccccgccgccaTAGCCCGGCCCCT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAgccgc                                                                 <  1:1002622/100‑1 (MQ=255)
                      cccccgcccccgccgccaTAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAgccgcc                                                                <  1:1828376/98‑1 (MQ=255)
                       cgccgccaccgccgccaTAGCCCGGGCCGT.CATCGGAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAgccgccg                                                               >  2:2978650/1‑101 (MQ=255)
                        gccgccaccgccgccaTAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAgccgccgc                                                              >  1:2237115/1‑101 (MQ=255)
                          cgcccccgccgcctTACCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCa                                                            <  1:3964379/101‑1 (MQ=255)
                          cgccaccgccgccaTAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCa                                                            >  2:2550035/1‑101 (MQ=255)
                              cccgccgcaaTCGCCGGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGACCCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATccc                                                        <  2:2683829/100‑1 (MQ=255)
                              ccccccgccaGAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGACCCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATccc                                                        <  2:313850/97‑1 (MQ=255)
                              accgccgccaTAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATccc                                                        >  1:4390373/1‑101 (MQ=255)
                              accgccgccaTAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATccc                                                        >  2:1832897/1‑101 (MQ=255)
                                cgccgccaTAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCgg                                                      >  2:1617162/1‑101 (MQ=255)
                                 gccgccaTAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgc                                                     >  2:3636590/1‑101 (MQ=255)
                                 gccgccaTAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCAAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgc                                                     <  1:1445827/101‑1 (MQ=255)
                                 ccccccaTACCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgc                                                     <  1:3096797/97‑1 (MQ=255)
                                   cgccaTAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccg                                                   >  1:2088305/1‑101 (MQ=255)
                                    gccaTAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccgc                                                  >  1:2178104/1‑101 (MQ=255)
                                     ccaTAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccgcc                                                 >  2:4320048/1‑101 (MQ=255)
                                      cagcgccggggcCGC.CACCGTCGCCGCCGCCATAGCCCCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCa                                                <  2:1699893/91‑1 (MQ=255)
                                      caTAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCa                                                >  1:3161590/1‑101 (MQ=255)
                                      caTAGCCCGGGCCGT.CACCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCa                                                <  1:3110860/101‑1 (MQ=255)
                                       aTAGCCGGGGCCTT.CATCGAACCCCCCCCCATAGCCGCCCCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCaa                                               <  2:692536/101‑1 (MQ=255)
                                        tAGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAag                                              >  1:2597301/1‑101 (MQ=255)
                                         aGCCCGGGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagc                                             >  2:3592793/1‑101 (MQ=255)
                                          cccgggcccGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagca                                            <  2:2445978/94‑1 (MQ=255)
                                             ggggcCGT.CACCGTAGCCCCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCg                                         <  1:3944713/98‑1 (MQ=255)
                                             cggaccGT.CACCGTAGCCGCCGCCATAGCCCCGGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCg                                         <  2:3539418/97‑1 (MQ=255)
                                               ggcCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGACCCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTc                                       <  2:3731572/100‑1 (MQ=255)
                                                gcCGTCCATCGAACCCGCCGCCATGGCCCCCGCCCTGCGACCCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTc                                       <  2:2522452/101‑1 (MQ=255)
                                                gcCGT.CTCCGTAGCCGCCCCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCa                                      <  2:1805611/101‑1 (MQ=255)
                                                gcCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCa                                      <  1:2336630/101‑1 (MQ=255)
                                                   gT.CCTCGTGGCCCCCGCCATGGCCGCCGCCCTGCGACCCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGt                                   <  2:4085861/101‑1 (MQ=255)
                                                    t.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGtt                                  >  1:2160886/1‑101 (MQ=255)
                                                    t.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGtt                                  >  2:3846492/1‑101 (MQ=255)
                                                     tcctCGTAGCCCCCCCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGtt                                  <  1:2148006/98‑1 (MQ=255)
                                                      cgtCGGAGCCGCCGCCATAGCCCCACCCCGGCGAACCGC.CG.CGGTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTg                                 <  2:1789901/99‑1 (MQ=255)
                                                       aTCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGc                                >  2:1243870/1‑101 (MQ=255)
                                                        tCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGcc                               >  1:2497860/1‑101 (MQ=255)
                                                         cgaggccgcccccATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGccc                              <  2:3924863/97‑1 (MQ=255)
                                                         cGTCGCCGCCGCCATACCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGccc                              <  2:1631600/101‑1 (MQ=255)
                                                         cGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGccc                              >  2:794681/1‑101 (MQ=255)
                                                         cGTAGCCGCCGCCATAGCCGCCGCCCGGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGccc                              <  2:2718928/101‑1 (MQ=255)
                                                          gtgcccccccccATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCg                             <  1:3532923/91‑1 (MQ=255)
                                                          gTAGCCCCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCg                             <  1:309553/101‑1 (MQ=255)
                                                            aGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGta                           >  1:2530393/1‑101 (MQ=255)
                                                            aGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGta                           >  1:65579/1‑101 (MQ=255)
                                                            aGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGta                           >  1:722374/1‑101 (MQ=255)
                                                            aGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGta                           >  2:1880677/1‑101 (MQ=255)
                                                             gcccccgccATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGtat                          <  1:2063861/101‑1 (MQ=255)
                                                             ccccccgccATAGCCGCCGCCCTGCGACCCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGtat                          <  2:1039407/97‑1 (MQ=255)
                                                                cccgccATAGCCGCCGCCCTGCGACCCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAgg                       <  1:3268748/100‑1 (MQ=255)
                                                                  cgccATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAgggg                     >  1:1980631/1‑101 (MQ=255)
                                                                  cgccAACGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAgggg                     <  2:4039321/101‑1 (MQ=255)
                                                                   gccATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgc                    >  2:2140793/1‑101 (MQ=255)
                                                                   gccATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgc                    <  2:4213568/101‑1 (MQ=255)
                                                                    ccATAGCCGCCGCCCTGCGAACCGC.CGCC.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcg                   >  2:3601159/1‑101 (MQ=255)
                                                                     cATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGCgcgc                  >  2:1159830/1‑101 (MQ=255)
                                                                     cATAGCCGCCGCCCTGCGAACCGC.CGCC.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcgc                  >  1:1228822/1‑101 (MQ=255)
                                                                      aTAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcgcg                 >  1:934041/1‑100 (MQ=255)
                                                                       tAGCCGCCCCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcag                <  2:2429465/101‑1 (MQ=255)
                                                                        aGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcagc               >  1:372596/1‑101 (MQ=255)
                                                                          ccgccgccCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCGCGGCCGCCAAGCAGCGTCAGGTTGACCGTATAGGGGCGCAGcac             >  2:116448/1‑101 (MQ=255)
                                                                          ccgccgccCTGCGAACCGC.CGCC.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcac             >  2:3880145/1‑101 (MQ=255)
                                                                           cgccgccCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcacc            >  1:56576/1‑101 (MQ=255)
                                                                            gccgccCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcacca           >  1:2087280/1‑101 (MQ=255)
                                                                            gccgccCTGCGAACCGC.CGCC.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcacca           >  2:1014130/1‑101 (MQ=255)
                                                                            gccgccCTGCGAACCGC.CGCC.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcacca           >  1:3475198/1‑101 (MQ=255)
                                                                             ctcccgcgCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcaccac          >  2:894262/8‑101 (MQ=255)
                                                                             ccgccCTGCGACCCCCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcaccac          <  1:147323/101‑1 (MQ=255)
                                                                              cgccCTGCAACCCGCCGC.C.GTAATTGCCCCCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCaccacc         <  2:507507/101‑1 (MQ=255)
                                                                               gccCTGCGAACCGC.CGCC.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCt        >  1:397339/1‑101 (MQ=255)
                                                                                cccTGCGACCCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTc       <  1:2550198/101‑1 (MQ=255)
                                                                                 cgtGCGAACAGCCGC.C.GTAATTGCCGGCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCACg      >  2:406409/3‑101 (MQ=255)
                                                                                 ccTGCGACCCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCg      <  1:3333202/101‑1 (MQ=255)
                                                                                 ccTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCg      >  2:4098470/1‑101 (MQ=255)
                                                                                 ccTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCg      >  1:259476/1‑101 (MQ=255)
                                                                                 ccTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCg      >  2:891580/1‑101 (MQ=255)
                                                                                     cGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCg  >  1:3705871/1‑101 (MQ=255)
                                                                                     cGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCg  >  1:2812226/1‑101 (MQ=255)
                                                                                               |                                                                                            
CCCGACGAGGGGGCC.GAGGAGCCGCCGCCACCGCCGCCATAGCCCGCGCCGT.CATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCGCG.C.GTAATTGCCACCGCCGCGCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCG  >  NC_014034/1951115‑1951298

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG