Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,976,892 G→C 85.7% intergenic (‑71/+9) fba ← / ← gap1 fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,976,8920G→C85.7% 44.1 ‑1.7 0.0133intergenic (‑71/+9)fba/gap1fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (77/37):  ref base (16/3):  total (93/40)
Fisher's exact test strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

CCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCGGCGTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976801‑1976990
                                                                                               |                                                                                                  
ccGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTtct                                                                                           >  1:4253162/1‑101 (MQ=255)
ccGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTtct                                                                                           >  2:2198822/1‑101 (MQ=255)
 cGAAGGGTGATCGGTGCCTTTTTCCCGTTTCCTTGGGTTCGCGGCCTTTTTCTTGGCCAAAATCCGCGGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTtctc                                                                                          <  2:1242968/101‑1 (MQ=18)
  gAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCa                                                                                         >  2:1400579/1‑101 (MQ=255)
    aGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CCGCCGGC.TTTTCTCAGa                                                                                       >  1:3682448/1‑101 (MQ=255)
    aGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGa                                                                                       >  1:3270935/1‑101 (MQ=255)
       gTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCa                                                                                    >  2:2485331/1‑101 (MQ=255)
         gATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGc                                                                                  >  1:896004/1‑101 (MQ=255)
         gATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGc                                                                                  >  2:1211597/1‑101 (MQ=255)
         gATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGc                                                                                  >  1:1981423/1‑101 (MQ=255)
          aTCAGTCCCTTTTTCTCATTTCCTGGAGTGCGCGGCCTTTTTCTTGGCCGAAATCCGCGGGGGGCCC.GGGGG.CGGCG.CC.CCCGGCTTTTCTCAGATCAGCt                                                                                 <  2:538684/101‑1 (MQ=255)
            cAGTGCTTTTTTCTCGTTTCTTTGGTTTCGGGGCTTTTTTCTGGCCAAAAACCCGCGGGGGGCCG.GGGGG.CCGCC.CC.CCCGGCTTTTCTCAGATCAGCTTg                                                                               <  1:325815/101‑1 (MQ=255)
            cAGTGCCTTTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCGGGGGGTCG.GGGGG.CAGCC.CC.CCCGGCTTTTCTCAGATCAGCTTg                                                                               <  2:1348490/101‑1 (MQ=255)
            cAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCTTTTTTCTGGGCCGAAATCCGCGGGGGGTCC.GGGGG.CAGCC.CC.CCCGGCTTTTCTCAGATCAGCTTg                                                                               <  2:3398066/101‑1 (MQ=25)
             aGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGc                                                                              >  1:1671949/1‑101 (MQ=255)
               tGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGccc                                                                            >  2:4412045/1‑101 (MQ=255)
                gCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCa                                                                           >  2:3767364/1‑101 (MQ=255)
                 cctttttCTCGGTTCCTTGGTTTCGGGCCTTTTTTTTTGCCCAAAATCCGCGGGGGGCCG.GGGGG.CGGCC.CC.CCCGGCTGTTCTCAGATCAGCTTGCCCAt                                                                          <  1:2549485/98‑1 (MQ=255)
                 cctttttCCCAGTTCCTTGAGTTCGCGGCTTTTTTTTTGGCCAAAATCCGCGGGGGGGCG.GGGGG.CGGCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCAt                                                                          <  1:1404501/98‑1 (MQ=255)
                 cccttttCTCAGTTCGTTGAGTTGCCGGCCTTTTTCTTGGCCGAAATCCGCGGGGGTTCG.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCAt                                                                          <  2:2775748/98‑1 (MQ=21)
                  ctttttCTCAGTTCCTGGATTTCGCGGCTTTTTTTTGGGCCAAAATCCGCGGGGGGCCC.GGGGG.CGGCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATc                                                                         <  1:1348927/99‑1 (MQ=255)
                    ttttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGc                                                                       >  1:3539315/1‑101 (MQ=255)
                     tttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGcc                                                                      >  1:2847899/1‑101 (MQ=255)
                      ttttcAGTTCTTTGATGTGGCGTCTTTTTTCTTGCCCAAAATCCGCGGGGGGCCG.GGGGG.CAGCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCa                                                                     <  2:982101/98‑1 (MQ=255)
                      ttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCa                                                                     >  1:1758423/1‑101 (MQ=255)
                       tctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAc                                                                    >  1:465203/1‑101 (MQ=255)
                       tctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAc                                                                    >  2:1952344/1‑101 (MQ=255)
                        ctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCAAAATCCGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcg                                                                   <  2:3984546/101‑1 (MQ=25)
                          cAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcggc                                                                 >  2:1158624/1‑101 (MQ=255)
                           aGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcggcg                                                                >  1:4167357/1‑101 (MQ=255)
                            gTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcggcgg                                                               >  1:4009095/1‑101 (MQ=255)
                              tCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             >  1:21511/1‑101 (MQ=255)
                              tCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             >  1:3702102/1‑101 (MQ=255)
                               ccTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATCCGCGGGGGGTCG.GGGGG.CAGCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTAt                                                            <  1:1005823/101‑1 (MQ=37)
                               ccTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGGAt                                                            >  1:4076581/1‑101 (MQ=37)
                                 ttGAGTGCGCGGCGTTTTTCTTGGCCGAATTACGCCGGGGGTCC.GGGGA.ATGCC.CC.CCGGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCg                                                          <  2:3627660/101‑1 (MQ=25)
                                  tGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg                                                         >  2:4029900/1‑101 (MQ=255)
                                    aGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGcc                                                       >  1:2216319/1‑101 (MQ=255)
                                     gTTCGCGGCTTTTTTCTTGGCCGAAATCCGCGGGGGGTCG.GGGGG.CAGCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCa                                                      <  1:1442704/101‑1 (MQ=25)
                                     gTTCGCGGCCTTTTTCTTGGCCGAAATAGGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACTGCGGTATCGGCCa                                                      >  1:4290325/1‑101 (MQ=37)
                                      ttCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCAt                                                     >  2:2035925/1‑101 (MQ=255)
                                       tCGCGGCTTTTTTCTTGGCCGAAATCCGCGGGGGGCCG.GGGGC.CGGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATg                                                    <  1:3257402/101‑1 (MQ=21)
                                       tCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATg                                                    <  2:2504221/101‑1 (MQ=255)
                                        cgcgGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGc                                                   >  1:2215012/1‑101 (MQ=255)
                                         gcgGCCTTTTTCTTGGCCGAAATCCGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCg                                                  <  2:4252893/101‑1 (MQ=37)
                                         gcgGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCg                                                  >  2:3151412/1‑101 (MQ=255)
                                          cgGCCTTTTTCTTGGCCGAAATCCGCCGGGGGTCC.GGGGG.CACCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCgg                                                 <  1:2099650/101‑1 (MQ=37)
                                           ggCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGt                                                >  1:2931962/1‑101 (MQ=255)
                                           gcccTTTTTTTTGGCCGAAGTGCGCGGGGGGTCG.GGGGG.CGGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGt                                                <  2:3629417/99‑1 (MQ=21)
                                             ccTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTc                                              >  2:4112429/1‑101 (MQ=255)
                                              cTTTTTCTTGGCCGAAAGACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg                                             <  2:1799254/101‑1 (MQ=37)
                                                tttttgtGGCCAAAAGCCGGCGGGGGGCCGGGGGG.CGCCC.CC.CCGGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCga                                            <  1:385690/95‑1 (MQ=12)
                                                 tttTTTGGCCAAAACCCGGCGGGGGGCC.GGGGGCCCCCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgag                                           <  1:1145065/101‑1 (MQ=12)
                                                  ttCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGaa                                         >  1:1413934/1‑101 (MQ=255)
                                                    ctgggCCGAAATACGGCGGGGGGGC.GGGGG.AGGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGc                                       <  1:556831/98‑1 (MQ=18)
                                                    cTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGc                                       >  2:3341610/1‑101 (MQ=255)
                                                    cTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGc                                       >  2:4403760/1‑101 (MQ=255)
                                                      tGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGccc                                     >  2:1700397/1‑101 (MQ=255)
                                                      gggCCGAAACACGCCGGCGGTCG.GGGGG.CAGCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGccc                                     <  2:4008839/100‑1 (MQ=21)
                                                         ccGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcat                                  >  2:4067036/1‑101 (MQ=255)
                                                          cGAAATGCGCGGGGGGTCC.GGGGG.CAGCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcatt                                 <  2:3577747/101‑1 (MQ=21)
                                                          cGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcatt                                 >  2:2316519/1‑101 (MQ=255)
                                                           gAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattc                                >  1:3516403/1‑101 (MQ=255)
                                                            aagtgccccGGTGTTCC.GGGGG.CCTCC.CC.CCAGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               <  2:414167/94‑1 (MQ=17)
                                                            aaaTACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               >  2:2024340/1‑101 (MQ=255)
                                                            aaaTACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               <  2:1380487/101‑1 (MQ=255)
                                                             aaTACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcat                              >  2:3080403/1‑101 (MQ=255)
                                                              agtggCCGGGCGGCCGGGGGG.CATCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcat                              <  2:2390684/97‑1 (MQ=17)
                                                              aacggcggggggGCA.GGGGG.CCCCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             <  1:4412317/94‑1 (MQ=17)
                                                              aTACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             >  2:4208792/1‑101 (MQ=255)
                                                              aTACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGGATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             >  1:1094212/1‑101 (MQ=25)
                                                               tccGCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTg                            <  1:2076705/99‑1 (MQ=255)
                                                                  gCCGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa                         >  1:829934/1‑101 (MQ=255)
                                                                   ccGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCAt                        >  2:3535756/1‑101 (MQ=255)
                                                                   ccGGGGGTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCAt                        >  1:2576423/1‑101 (MQ=255)
                                                                    cGGGGGTCCGGGGGG.CGGCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCAt                        <  2:3270703/101‑1 (MQ=18)
                                                                    cGGGGGTCC.GGGGG.CACCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATa                       <  2:3686183/101‑1 (MQ=21)
                                                                      ggggTGG.GGGGG.CCGCC.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAcc                     <  1:3925711/101‑1 (MQ=18)
                                                                      ggggTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAcc                     >  1:4160524/1‑101 (MQ=255)
                                                                        ggTCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCAc                   <  2:2199306/101‑1 (MQ=255)
                                                                          tCC.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGa                 >  2:1075909/1‑101 (MQ=255)
                                                                          gtcgagggg.CAGCGCCC.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACg                  >  2:3769707/6‑101 (MQ=18)
                                                                           cc.GGGGG.CAGCGTCC.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGAc                >  1:2426111/1‑101 (MQ=255)
                                                                           cc.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGAc                >  2:1047456/1‑101 (MQ=255)
                                                                            c.GGGGG.CAGCGCCC.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACa               >  1:2641108/1‑101 (MQ=255)
                                                                            c.GGGGG.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACa               <  1:1211690/101‑1 (MQ=255)
                                                                               gggg.CAGCGCCC.CCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAgg             >  2:2756051/1‑101 (MQ=255)
                                                                                 gg.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGAt           <  1:1965219/101‑1 (MQ=255)
                                                                                  g.CAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATg          <  2:2193107/101‑1 (MQ=255)
                                                                                    cAGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         >  1:2282151/1‑101 (MQ=255)
                                                                                     ccgcccc.ccGGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         <  1:1928314/96‑1 (MQ=255)
                                                                                     aGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGNATGCg        >  1:1311991/1‑101 (MQ=255)
                                                                                     aGCG.CC.CCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCg        >  1:696632/1‑101 (MQ=255)
                                                                                       cgccc.ccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGa      >  2:208066/4‑101 (MQ=255)
                                                                                        gccc.ccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAc     >  1:2261707/3‑101 (MQ=255)
                                                                                         ccc.ccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAcc    >  2:1535623/2‑101 (MQ=255)
                                                                                          cc.ccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCa   >  1:1576285/1‑101 (MQ=255)
                                                                                          cc.ccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCa   >  2:983370/1‑101 (MQ=255)
                                                                                           c.ccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAt  <  1:3361772/101‑1 (MQ=255)
                                                                                           c.ccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAt  <  2:856124/101‑1 (MQ=255)
                                                                                               |                                                                                                  
CCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCC.GGGGG.CAGCG.CC.CCGGCGTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976801‑1976990

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG