Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,990,962 T→A 100% Y555F (TAC→TTC)  glgB ← 1,4‑alpha‑glucan branching protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,990,9620T→A100.0% 880.4 220Y555F (TAC→TTC) glgB1,4‑alpha‑glucan branching protein
Reads supporting (aligned to +/- strand):  new base (106/114):  ref base (0/0):  total (106/114)

Only 100 of 221 total aligned reads displayed.

GAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGTACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGACT  >  NC_014034/1990864‑1991059
                                                                                                  |                                                                                                 
gAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAAc                                                                                                 <  2:412397/101‑1 (MQ=255)
gAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAAc                                                                                                 <  2:1934572/101‑1 (MQ=255)
 aaGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACg                                                                                                >  1:2966129/1‑101 (MQ=255)
   ggTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCa                                                                                              >  2:3676146/1‑101 (MQ=255)
    gTGCCAGTCAAGGGCCCCCTGACGCGCCCATTCGCGCCCCTGCGCTAACTCCGACCCCATCAAGAGCAGCTTCTTGCCCGGATTCGCCCATTGGAACGCAt                                                                                             <  2:527004/101‑1 (MQ=255)
     tGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATa                                                                                            <  2:3760532/101‑1 (MQ=255)
     tGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATa                                                                                            >  1:1051063/1‑101 (MQ=255)
     tGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATa                                                                                            <  2:1854465/101‑1 (MQ=255)
     tGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATa                                                                                            >  2:241957/1‑101 (MQ=255)
       ccAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGa                                                                                          <  2:3420311/101‑1 (MQ=255)
       ccAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGa                                                                                          <  1:946171/101‑1 (MQ=255)
       ccAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGa                                                                                          <  1:206777/101‑1 (MQ=255)
       ccAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGa                                                                                          <  1:1904342/101‑1 (MQ=255)
         aGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAg                                                                                        <  2:4324229/101‑1 (MQ=255)
          gTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAgg                                                                                       >  2:3610357/1‑101 (MQ=255)
            cAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGcc                                                                                     <  2:2627876/101‑1 (MQ=255)
            cAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGcc                                                                                     >  2:4407347/1‑101 (MQ=255)
             aaGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGccc                                                                                    >  1:1878908/1‑101 (MQ=255)
              aaggCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAGCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCg                                                                                   <  2:4428582/99‑1 (MQ=255)
              aGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCg                                                                                   <  2:4061194/101‑1 (MQ=255)
               gggCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCTCGGATGCGCCCATTGGAACGCATCGAAGGACCGc                                                                                  >  2:2764660/1‑101 (MQ=255)
                ggCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCa                                                                                 >  1:683857/1‑101 (MQ=255)
                ggCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCa                                                                                 >  1:1320452/1‑101 (MQ=255)
                 gCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAg                                                                                <  2:4392835/101‑1 (MQ=255)
                  cccccTGAAGCGCCCATCCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAgg                                                                               <  2:826219/101‑1 (MQ=255)
                    cccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGtt                                                                             <  2:2803503/101‑1 (MQ=255)
                     ccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTg                                                                            <  2:2156204/101‑1 (MQ=255)
                       tGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGc                                                                          >  2:1615649/1‑101 (MQ=255)
                       tGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGc                                                                          <  1:1521500/101‑1 (MQ=255)
                       tGATGCGCACATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGc                                                                          <  1:3692808/101‑1 (MQ=255)
                        gATGCGCCCATTCGCGCTCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCg                                                                         <  2:2220963/101‑1 (MQ=255)
                        gATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCg                                                                         >  2:2978091/1‑101 (MQ=255)
                          tGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGaa                                                                       >  1:786322/1‑101 (MQ=255)
                             gcCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAAttt                                                                    <  1:2695673/101‑1 (MQ=255)
                              cccATTCGCGCCCCTGCGCGAGCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTc                                                                   <  2:3291764/101‑1 (MQ=255)
                               ccATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCt                                                                  <  1:3146183/101‑1 (MQ=255)
                                cATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTg                                                                 >  1:3399893/1‑101 (MQ=255)
                                 aTTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGc                                                                >  2:3108401/1‑101 (MQ=255)
                                  ttCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCa                                                               <  1:2036164/101‑1 (MQ=255)
                                     gcgcCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAAcc                                                            <  1:1093640/101‑1 (MQ=255)
                                      cgcCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAAccc                                                           >  2:237940/1‑101 (MQ=255)
                                         cccTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGtc                                                        <  1:3881994/101‑1 (MQ=255)
                                         cccTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGtc                                                        <  1:2518250/101‑1 (MQ=255)
                                             gcgcGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTccc                                                    >  2:1259670/1‑101 (MQ=255)
                                                cgAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGc                                                 >  2:683245/1‑101 (MQ=255)
                                                cgAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCAAATTTCTGAAACCGGTCACCCGGc                                                 >  2:1768331/1‑101 (MQ=255)
                                                 gAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCa                                                >  1:1620419/1‑101 (MQ=255)
                                                   aCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATc                                              <  2:3480523/101‑1 (MQ=255)
                                                    cTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCTCGGCATCt                                             >  1:2683502/1‑101 (MQ=255)
                                                      ccTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTg                                           <  1:2562922/101‑1 (MQ=255)
                                                       cTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGc                                          >  2:4002281/1‑101 (MQ=255)
                                                        tGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGcc                                         >  2:2758841/1‑101 (MQ=255)
                                                        tGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGcc                                         <  2:4259854/101‑1 (MQ=255)
                                                         gCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGccc                                        >  2:2819591/1‑101 (MQ=255)
                                                             cATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCa                                    <  2:1106803/101‑1 (MQ=255)
                                                              aTGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCAt                                   >  1:3538360/1‑101 (MQ=255)
                                                              aTGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCAt                                   >  1:631989/1‑101 (MQ=255)
                                                              aTGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCAt                                   <  2:1000914/101‑1 (MQ=255)
                                                              aTGAAGAGCAGCTTCTTGCCCGGACGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTCCAACCCGTCTCCCGGCATCTTGCCCAGCAt                                   <  1:3971654/101‑1 (MQ=255)
                                                               tGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATc                                  <  2:1187603/101‑1 (MQ=255)
                                                               tGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATc                                  >  2:210846/1‑101 (MQ=255)
                                                                 aaGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGa                                <  1:1562353/101‑1 (MQ=255)
                                                                 aaGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGa                                >  2:1972745/1‑101 (MQ=255)
                                                                 aaGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGa                                >  1:1215659/1‑101 (MQ=255)
                                                                 aaGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGa                                >  2:1365864/1‑101 (MQ=255)
                                                                 aaGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGa                                >  1:4154223/1‑101 (MQ=255)
                                                                  agagCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGAc                               >  2:2475101/1‑101 (MQ=255)
                                                                   gagCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGAcc                              >  1:4093298/1‑101 (MQ=255)
                                                                   gagCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGAcc                              <  2:729851/101‑1 (MQ=255)
                                                                     gcagcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGAcccc                            <  1:2346604/101‑1 (MQ=255)
                                                                     gcagcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGAcccc                            >  1:2232956/1‑101 (MQ=255)
                                                                       agcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCtt                          <  1:3011778/101‑1 (MQ=255)
                                                                       agcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCtt                          <  2:321118/101‑1 (MQ=255)
                                                                       agcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCtt                          >  1:3026149/1‑101 (MQ=255)
                                                                        gcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTg                         >  2:1297957/1‑101 (MQ=255)
                                                                         cttcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGc                        <  2:156088/101‑1 (MQ=255)
                                                                         cttcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGc                        >  1:3724735/1‑101 (MQ=255)
                                                                          ttcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGcc                       <  2:2691558/101‑1 (MQ=255)
                                                                           tcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCg                      <  2:3308210/101‑1 (MQ=255)
                                                                           tcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCg                      <  1:237967/101‑1 (MQ=255)
                                                                            cttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGt                     <  2:2938967/101‑1 (MQ=255)
                                                                               gCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGaa                  <  1:3775860/101‑1 (MQ=255)
                                                                                 ccGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCACGGCATCTTGCCCAGCATCGACCCCTTGCAGTGAAcc                >  2:2043200/1‑100 (MQ=255)
                                                                                    gATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGAcc             <  2:3888180/101‑1 (MQ=255)
                                                                                     aTGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCt            <  2:570975/101‑1 (MQ=255)
                                                                                     aTGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCt            >  2:1710537/1‑101 (MQ=255)
                                                                                     aTGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCt            >  2:1382653/1‑101 (MQ=255)
                                                                                      tGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCtc           >  1:2444972/1‑101 (MQ=255)
                                                                                       gcgcCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCtcg          <  1:3277620/101‑1 (MQ=255)
                                                                                       gcgcCCATTGGAACGCATAGAAGGCCCGCAGGCTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCTACCCCTTGCCGTGAACGACAtcg          >  2:2316352/1‑101 (MQ=255)
                                                                                         gcCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGAAAACCGACTCCAGGCATCTTGCCCAGCATAGACCCCTTGCCGTGAACGACCtcctc        >  1:1436943/1‑101 (MQ=255)
                                                                                          cccATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCtcgtcg       >  1:1000661/1‑101 (MQ=255)
                                                                                          cccATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGGGAACGACCtcgtcg       >  1:2802591/1‑101 (MQ=255)
                                                                                           ccATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTcgtcgt      >  2:3804487/1‑101 (MQ=255)
                                                                                           ccATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTcgtcgt      >  1:4245241/1‑101 (MQ=255)
                                                                                           ccATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTcgtcgt      <  2:692122/101‑1 (MQ=255)
                                                                                             aTTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTgc    >  1:1019363/1‑100 (MQ=255)
                                                                                               tGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGACt  <  2:2836117/101‑1 (MQ=255)
                                                                                               tGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGACt  <  1:3124673/101‑1 (MQ=255)
                                                                                               tGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGACt  >  1:1858363/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGTACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGACT  >  NC_014034/1990864‑1991059

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG