Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,040,375 C→G 3.3% intergenic (‑123/‑265) RCAP_rcc01888 ← / → RCAP_rcc01889 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,040,3750C→G 3.3% 7.1 0.0 ‑0.0209intergenic (‑123/‑265)RCAP_rcc01888/RCAP_rcc01889hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/4):  ref base (101/101):  total (104/105)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.53e-01

Only 100 of 229 total aligned reads displayed.

CCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGTGGGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GG.CGCGCCATAAGTGAGCCCACCCGGTTCGGAGGGTTGGATGCATAAATC  >  NC_014034/2040276‑2040476
                                                                                                   |                                                                                                        
ccGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgg                                                                                                        <  2:2472/101‑1 (MQ=255)
  ggCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgggc                                                                                                      >  2:3338106/1‑100 (MQ=255)
  ggCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgggc                                                                                                      >  1:1167577/1‑100 (MQ=255)
  ggCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgggc                                                                                                      >  1:1743630/1‑100 (MQ=255)
      tctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGCTCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGAt                                                                                                 >  2:1141883/1‑101 (MQ=255)
      tctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGAt                                                                                                 <  2:2349555/101‑1 (MQ=255)
      tctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGAt                                                                                                 >  1:1541231/1‑101 (MQ=255)
       ctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATg                                                                                                <  2:1983814/101‑1 (MQ=255)
        tGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATgg                                                                                               <  1:3591292/101‑1 (MQ=255)
         gggTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGGCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGc                                                                                              >  1:3677420/1‑101 (MQ=255)
            ttGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTa                                                                                           <  1:3074632/101‑1 (MQ=255)
            ttGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTa                                                                                           <  1:4286765/101‑1 (MQ=255)
             tGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAc                                                                                          >  2:863121/1‑101 (MQ=255)
             tGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAc                                                                                          >  2:650167/1‑101 (MQ=255)
               ggATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAcgc                                                                                        >  2:340935/1‑101 (MQ=255)
                gATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAcgcg                                                                                       <  1:3360323/101‑1 (MQ=255)
                gATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAcgcg                                                                                       >  1:2782981/1‑101 (MQ=255)
                  tGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGCTGGCTTACGCGGt                                                                                     >  1:2987312/1‑101 (MQ=255)
                  tGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGt                                                                                     <  1:1364049/101‑1 (MQ=255)
                   ggAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTa                                                                                    >  2:4038793/1‑101 (MQ=255)
                    gAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTAt                                                                                   <  1:2627163/101‑1 (MQ=255)
                    gAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTAt                                                                                   <  1:3859816/101‑1 (MQ=255)
                        aaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGgcg                                                                               <  1:3338343/101‑1 (MQ=255)
                        aaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGgcg                                                                               >  2:3495619/1‑101 (MQ=255)
                        aaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGgcg                                                                               <  2:2097953/101‑1 (MQ=255)
                         aGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGgcgc                                                                              <  2:31201/101‑1 (MQ=255)
                          gACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGcgcg                                                                             <  2:2598454/101‑1 (MQ=255)
                            ccGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTg                                                                           >  2:1663331/1‑101 (MQ=255)
                               gAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCa                                                                        >  2:3089257/1‑101 (MQ=255)
                                aaCGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCaa                                                                       >  1:25188/1‑101 (MQ=255)
                                 aCGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCaaa                                                                      <  1:275641/101‑1 (MQ=255)
                                   ggCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGa                                                                    <  1:340970/101‑1 (MQ=255)
                                   ggCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGa                                                                    >  2:4314271/1‑101 (MQ=255)
                                   ggCCAGGGGCCAGACCCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGa                                                                    <  2:1697382/101‑1 (MQ=255)
                                    gCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAc                                                                   <  1:901178/101‑1 (MQ=255)
                                    gCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAc                                                                   >  1:351702/1‑101 (MQ=255)
                                    gCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAc                                                                   <  2:2850712/101‑1 (MQ=255)
                                     ccAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcg                                                                  <  1:762501/101‑1 (MQ=255)
                                     aggtgatccAGCCGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcg                                                                  >  2:1017867/8‑101 (MQ=255)
                                       aGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcggc                                                                >  1:4128426/1‑101 (MQ=255)
                                         gggCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCTGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcggcgg                                                              >  1:581977/1‑101 (MQ=255)
                                         gggCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcggcgg                                                              >  1:3247331/1‑101 (MQ=255)
                                          ggCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACggcggc                                                             <  1:2147048/101‑1 (MQ=255)
                                          ggCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACggcggc                                                             <  1:1413659/101‑1 (MQ=255)
                                           gCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGcc                                                            <  2:739065/101‑1 (MQ=255)
                                           gCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGcc                                                            >  1:2398927/1‑101 (MQ=255)
                                            ccAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCg                                                           <  1:3786456/101‑1 (MQ=255)
                                             cAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGc                                                          >  1:29402/1‑101 (MQ=255)
                                             cAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGc                                                          >  1:2844584/1‑101 (MQ=255)
                                             cAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGc                                                          >  2:2305132/1‑101 (MQ=255)
                                              aGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGGGCCAAAGACGGCGGCCGC.a                                                        >  1:4424088/1‑101 (MQ=255)
                                                  gCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.gg                                                   >  2:2353160/1‑101 (MQ=255)
                                                  gCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.gg                                                   >  1:2664289/1‑101 (MQ=255)
                                                  gCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.gg                                                   <  2:3674169/101‑1 (MQ=255)
                                                   cAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.Gg.c                                                 >  1:1978879/1‑101 (MQ=255)
                                                          tgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGcc                                            <  2:448458/100‑1 (MQ=255)
                                                          tgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGcc                                            <  2:91266/100‑1 (MQ=255)
                                                          tgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGcc                                            <  1:585279/100‑1 (MQ=255)
                                                           gggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCa                                           <  1:1097310/101‑1 (MQ=255)
                                                           gggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCa                                           <  2:3029285/101‑1 (MQ=255)
                                                           gggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCa                                           <  1:4314540/101‑1 (MQ=255)
                                                               gATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAg                                       <  2:2733675/101‑1 (MQ=255)
                                                                 tCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTg                                     <  2:1604769/101‑1 (MQ=255)
                                                                 tCCGGTCGAAGGTCAGACATTGTGCAAAAGCA.CCGGGCCGGATGGCTTACGCGGTATGGCGCGTGTCAAAGACCGCGGCCGC.AAA.GGCCCCGGCATAAGTg                                     <  2:2987091/101‑1 (MQ=255)
                                                                 gccGGTAGAAGGTCAGACGTTGTGCAAAAGC.CCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCTGCCGC.AAA.GGCCGCGCCATAAGTg                                     <  2:2149204/100‑1 (MQ=255)
                                                                  ccGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGa                                    <  2:2125946/101‑1 (MQ=255)
                                                                  ccGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGa                                    <  1:29816/101‑1 (MQ=255)
                                                                   cGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAg                                   >  1:1441569/1‑101 (MQ=255)
                                                                    ggTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGC.AAA.GGCCGCGCCATAAGTGAGc                                  >  1:977178/1‑101 (MQ=255)
                                                                     gTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGcc                                 >  1:2602000/1‑101 (MQ=255)
                                                                     gTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGACGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGCCCGC.AAA.GGCCCCCCCATAAGTGAGcc                                 >  2:530895/1‑101 (MQ=255)
                                                                       cGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCa                               <  2:3240908/101‑1 (MQ=255)
                                                                        gAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCAc                              <  2:1441462/101‑1 (MQ=255)
                                                                        gAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCAc                              >  1:3997711/1‑101 (MQ=255)
                                                                         aaGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCAcc                             >  2:2501289/1‑101 (MQ=255)
                                                                         aaGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCAcc                             >  1:18267/1‑101 (MQ=255)
                                                                          aGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCAccg                            <  1:2304063/101‑2 (MQ=255)
                                                                               aGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCg                      >  2:3144760/1‑101 (MQ=255)
                                                                                gACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCgg                     >  2:1924607/1‑101 (MQ=255)
                                                                                gACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCgg                     <  1:4214764/101‑1 (MQ=255)
                                                                                gACGGTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCgg                     >  2:3767513/1‑101 (MQ=255)
                                                                                 aCGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGa                    <  2:181498/101‑1 (MQ=255)
                                                                                  cGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAg                   <  2:493580/101‑1 (MQ=255)
                                                                                    ttGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCGAAC.TC.CGCGCCATAAGTGAGCCCA.CCGGTTCGGAggt                 >  1:3810318/1‑100 (MQ=255)
                                                                                    ttGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGACCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggt                 >  2:1775932/1‑100 (MQ=255)
                                                                                     tgtgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggtt                <  2:4219660/101‑3 (MQ=255)
                                                                                       tgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggttgg              <  1:3144259/101‑5 (MQ=255)
                                                                                       tgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggttgg              <  2:4238664/101‑5 (MQ=255)
                                                                                       tgCAAAAGCA.CCGGGGCGGATGGCTTAAGCGGTATGGCGCGTGCCAAAGACGTAGGCCGC.AAACGC.CGCGCCATAAGTGAGCCCA.CTGGTTCGGAggttgg              >  2:2978642/1‑97 (MQ=255)
                                                                                        gCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGa            <  2:1372366/101‑1 (MQ=255)
                                                                                         cAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAGGGtggat            >  1:3157899/1‑97 (MQ=255)
                                                                                           aaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGc         >  1:2692113/1‑101 (MQ=255)
                                                                                           aaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGc         >  2:2635581/1‑101 (MQ=255)
                                                                                              agcaccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCAt       >  1:994068/5‑101 (MQ=255)
                                                                                               gcaccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATa      >  1:2258681/4‑101 (MQ=255)
                                                                                                caccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATaa     <  2:950220/99‑1 (MQ=255)
                                                                                                 accGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATaaa    >  1:2386149/2‑101 (MQ=255)
                                                                                                  ccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAAt   <  1:4184034/101‑1 (MQ=255)
                                                                                                   cGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATc  >  2:2593842/1‑101 (MQ=255)
                                                                                                   cGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAATc  >  2:1325311/1‑101 (MQ=255)
                                                                                                   |                                                                                                        
CCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGTGGGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGC.AAA.GG.CGCGCCATAAGTGAGCCCACCCGGTTCGGAGGGTTGGATGCATAAATC  >  NC_014034/2040276‑2040476

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG