Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,057,834 A→C 16.8% intergenic (‑600/‑302) RCAP_rcc01900 ← / → RCAP_rcc01901 hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,057,8340A→C16.8% 61.2 ‑0.2 ‑0.5137intergenic (‑600/‑302)RCAP_rcc01900/RCAP_rcc01901hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (13/10):  ref base (60/54):  total (73/64)
Fisher's exact test strand distribution p-value = 8.21e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.03e-01

Only 100 of 147 total aligned reads displayed.

CTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CCGCG.CGC.AGC.G.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057735‑2057913
                                                                                                      |                                                                                  
cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGc                                                                                  >  2:3675111/1‑101 (MQ=255)
cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGc                                                                                  >  2:1518090/1‑101 (MQ=255)
   tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaag                                                                               <  1:3015557/101‑4 (MQ=255)
    ttACCGCCCATTAGCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagc                                                                              <  2:1800576/101‑5 (MQ=255)
    ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagc                                                                              >  1:1085769/1‑97 (MQ=255)
    ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagc                                                                              >  2:3459715/1‑97 (MQ=255)
    ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagc                                                                              <  1:1898453/101‑5 (MQ=255)
     tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcg                                                                             >  1:1278645/1‑96 (MQ=255)
     tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcg                                                                             >  1:4435652/1‑96 (MQ=255)
      aCCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgt                                                                            >  1:821461/1‑95 (MQ=255)
       ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtt                                                                           <  2:2256118/101‑8 (MQ=255)
       ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtt                                                                           <  1:3630583/101‑8 (MQ=255)
        cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgttt                                                                          >  1:2695265/1‑93 (MQ=255)
        cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgttt                                                                          >  1:369408/1‑93 (MQ=255)
        cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgttt                                                                          >  2:449994/1‑93 (MQ=255)
         gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtttc                                                                         <  1:4265518/101‑10 (MQ=255)
         gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtttc                                                                         >  2:2014778/1‑92 (MQ=255)
                aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCg                                                                     >  1:887469/1‑101 (MQ=255)
                aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCg                                                                     >  2:1645822/1‑101 (MQ=255)
                 aCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCgg                                                                    <  1:668919/101‑1 (MQ=255)
                 aCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGCGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCgg                                                                    <  1:3111122/101‑1 (MQ=255)
                  cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGc                                                                   >  1:1022663/1‑101 (MQ=255)
                    cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCAt                                                                 <  2:4103195/101‑1 (MQ=255)
                    cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCAt                                                                 <  2:1362119/101‑1 (MQ=255)
                    cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCAt                                                                 <  1:1761062/101‑1 (MQ=255)
                     tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCacc                                                                >  2:945897/1‑99 (MQ=255)
                     tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATc                                                                >  1:1562326/1‑101 (MQ=255)
                     tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATc                                                                >  1:2628706/1‑101 (MQ=255)
                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCa                                                               <  1:2922857/101‑1 (MQ=255)
                         tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTc                                                            <  2:821379/101‑1 (MQ=255)
                         tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTc                                                            >  1:2235624/1‑101 (MQ=255)
                         tCGGCGCGCGGAATTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTc                                                            >  1:3191686/1‑101 (MQ=255)
                          cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCt                                                           >  2:1397781/1‑101 (MQ=255)
                           ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCtt                                                          <  1:897343/101‑1 (MQ=255)
                           ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCtt                                                          <  1:2810517/101‑1 (MQ=255)
                           ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCtt                                                          >  1:2388367/1‑101 (MQ=255)
                            gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTg                                                         <  2:2628535/101‑1 (MQ=255)
                            gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTg                                                         >  2:3223001/1‑101 (MQ=255)
                            ccgatctgACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTg                                                         <  2:3594211/94‑1 (MQ=255)
                              gcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.c                                                      <  2:937613/101‑1 (MQ=255)
                              gcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.c                                                      <  1:3171291/101‑1 (MQ=255)
                               cgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.cc                                                     <  2:3311765/101‑1 (MQ=255)
                                 cgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.cccc                                                   <  1:3369282/101‑1 (MQ=255)
                                   gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTgagatcgg                                                  >  1:3594459/1‑94 (MQ=255)
                                   gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.cccccc                                                 >  1:33876/1‑100 (MQ=255)
                                   gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.cccccc                                                 >  2:3401969/1‑100 (MQ=255)
                                    gcTTCGGGGCGGCGGGGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.ccccccg                                                <  2:3990397/100‑3 (MQ=255)
                                    acgtCGGGGCGGCGTTGGGCCGCTTGCGATCCTGCGTTTCCGGTTTGTCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGT.ccccccg                                                <  2:2212249/98‑3 (MQ=255)
                                    aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.ccccccg                                                >  2:1498884/1‑99 (MQ=255)
                                      ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                              >  1:3102527/1‑97 (MQ=255)
                                      ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                              >  2:1345596/1‑97 (MQ=255)
                                         ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGG.TTCCGAACCGGCATCATTCTTGG.CCCCCCCCcggg                                           >  1:1844841/1‑100 (MQ=255)
                                             cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACaa                                        >  2:3263529/1‑101 (MQ=255)
                                              ggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAAt                                       >  2:1009214/1‑101 (MQ=255)
                                              ggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAAt                                       >  1:3487144/1‑101 (MQ=255)
                                               gcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATc                                      <  1:1045744/101‑1 (MQ=255)
                                                tggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCa                                     <  1:154243/100‑1 (MQ=255)
                                                cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACGGGCATCATTCTTGGCCCCCCGCCGGGACAATCa                                     >  2:3177712/1‑101 (MQ=255)
                                                cggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCa                                     >  2:396294/1‑101 (MQ=255)
                                                 ggcggGGTTCTTGCGATCCTGCGTATCCGGATTGTCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCaa                                    >  2:154222/1‑100 (MQ=255)
                                                 ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    <  2:172016/101‑1 (MQ=255)
                                                 ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    <  2:160037/101‑1 (MQ=255)
                                                 ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    >  1:2303097/1‑101 (MQ=255)
                                                   cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACgg                                  <  1:4435145/101‑1 (MQ=255)
                                                   cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACgg                                  >  2:2419610/1‑101 (MQ=255)
                                                   cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAAgg                                  >  2:2854038/1‑101 (MQ=255)
                                                     gggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgcc                                <  1:1150171/101‑1 (MQ=255)
                                                      ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccg                               >  1:2491620/1‑101 (MQ=255)
                                                        ctctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACAgccgcc                             >  1:2944158/1‑101 (MQ=255)
                                                         tctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCt                            >  2:3208340/1‑101 (MQ=255)
                                                         tctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCt                            >  2:4089906/1‑101 (MQ=255)
                                                         tctTGCGATCCTCCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCt                            >  1:355174/1‑101 (MQ=255)
                                                          ctggtggctgtGCGTCTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGATCCGGCAACGTTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           <  2:3036260/91‑1 (MQ=255)
                                                            tGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         <  1:3340771/101‑1 (MQ=255)
                                                            tGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  1:1989796/1‑101 (MQ=255)
                                                              cGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       <  1:1434066/101‑1 (MQ=255)
                                                              cGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       <  1:1409731/101‑1 (MQ=255)
                                                              cGATCCTGCGTTGCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCTCCTCATTg                       <  2:2668656/101‑1 (MQ=255)
                                                                 tCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  2:1593571/1‑101 (MQ=255)
                                                                 tCCTGCGTTTCCGGATGGGCGACAGTCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    <  2:1098116/101‑1 (MQ=255)
                                                                  ccTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   >  1:214029/1‑101 (MQ=255)
                                                                   cTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCg                  >  2:1399377/1‑101 (MQ=255)
                                                                     gCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                >  1:4227015/1‑101 (MQ=255)
                                                                      cGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa               <  2:824082/101‑1 (MQ=255)
                                                                       gTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  1:2103178/1‑101 (MQ=255)
                                                                        tttCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             >  2:4247806/1‑101 (MQ=255)
                                                                           ccGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg          >  2:1223077/1‑101 (MQ=255)
                                                                            cGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGa         >  2:120783/1‑101 (MQ=255)
                                                                            cGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGa         >  1:4193843/1‑101 (MQ=255)
                                                                             ggATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  1:1727618/1‑101 (MQ=255)
                                                                              gATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       <  1:3038612/101‑1 (MQ=255)
                                                                               aTTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      <  2:1022578/101‑1 (MQ=255)
                                                                               aTTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      >  1:629665/1‑101 (MQ=255)
                                                                                ttGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     <  1:4121455/101‑1 (MQ=255)
                                                                                 tGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    >  1:3604498/1‑101 (MQ=255)
                                                                                 tGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    >  1:4284959/1‑101 (MQ=255)
                                                                                 tGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    <  2:2431800/101‑1 (MQ=255)
                                                                                  ggCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCCTTGTTCCGCCATTGCGATCAAAg   >  1:411341/1‑101 (MQ=255)
                                                                                   gCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  <  1:2793532/101‑1 (MQ=255)
                                                                                   gCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  >  2:109513/1‑101 (MQ=255)
                                                                                                      |                                                                                  
CTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CCGCG.CGC.AGC.G.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057735‑2057913

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG