Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,440 G→C 9.8% S2T (AGC→ACC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4400G→C 9.8% 43.9 ‑0.2 ‑0.2204S2T (AGC→ACC) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (11/9):  ref base (93/91):  total (104/100)
Fisher's exact test strand distribution p-value = 8.15e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.14e-01

Only 100 of 208 total aligned reads displayed.

CCGCCGACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAC  >  NC_014034/2155340‑2155539
                                                                                                     |                                                                                                   
ccgccgCCCCGGCGGCGGTCCCCCTCCCCTTCTGATGCCCTC.GGGCGCCGTCACCGTCCCCGCCCCCCGGGCGACAAGCCGCGAAAGGGCAGGATCATGAg                                                                                                     <  2:2967586/101‑1 (MQ=255)
 cgccgCCCCGGCCGCGGCCCCCCTCCCCTTCTGATGCCCCC.GGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGc                                                                                                    <  2:3512697/101‑1 (MQ=255)
  gccgCCCCGGCCGCGTTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGCCACCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCg                                                                                                   <  2:28486/101‑1 (MQ=255)
  gccgACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCg                                                                                                   >  1:2870333/1‑101 (MQ=255)
  gccgACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCg                                                                                                   >  2:606180/1‑101 (MQ=255)
   ccgCCCCGGCCGCGGTCCCCCTCACCTTCTGATCCCCTC.GGGCGCCGTCACCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGa                                                                                                  <  1:708413/101‑1 (MQ=255)
     gccccGGCCGGGGCCCCCCTCCCCTTCTGATTCCCCC.GGGCGCCGACCCCGCCCCCCCCCCCGGGGCGGACAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                <  1:3972563/99‑1 (MQ=255)
     gccccGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                <  2:2596505/99‑1 (MQ=255)
     gACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                >  2:907690/1‑101 (MQ=255)
       ccggcccGCGGCCGCCCTCCCTTTCGGATGCCCTG.GGGCCCCGTCCCCGCCCCCGCCGGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                              <  1:272185/96‑1 (MQ=255)
       cccGGCCGCGGGCCCCCTCCCCTTCTGATGCCCTC.GGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                              <  2:1752315/101‑1 (MQ=255)
        ccGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCaa                                                                                             >  1:4181099/1‑101 (MQ=255)
        ccGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCaa                                                                                             >  2:1649899/1‑101 (MQ=255)
         gggCCGGGGCCCCCCTCCCCTTCTGATGCCCTG.GGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAg                                                                                            <  1:2775229/100‑1 (MQ=255)
          ggCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgg                                                                                           >  1:2104105/1‑101 (MQ=255)
           gCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAggg                                                                                          >  1:4431665/1‑101 (MQ=255)
            ccGCGGCCGCCCTCCCCTTCTGAGGCCCGC.GGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgggg                                                                                         <  2:2570500/101‑1 (MQ=255)
             cgcgGCCCCCCTCCCCTTCTGACCCCCTC.GGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGc                                                                                        <  1:3421433/101‑1 (MQ=255)
              ccggccccccTCCCCTTCTGATCCCCTC.GGGCGCCGCCACCGCCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGcc                                                                                       <  2:484176/94‑1 (MQ=255)
                 gTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                    >  1:3002544/1‑101 (MQ=255)
                 gTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                    >  2:788239/1‑101 (MQ=255)
                 gTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                    >  1:50067/1‑101 (MQ=255)
                 gTCCCCCTCCCCTTCTGATCCCCTC.GGGCGCCGTCCCCGTCCCCGCCCGCCGGGGGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                    <  2:2814505/101‑1 (MQ=255)
                 gTCCCCCTCCCCTTCTGATCCCCCC.GGGCGCCGTCACCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                    <  1:2645760/101‑1 (MQ=255)
                  tccccccccccTTCTGATCCCCCC.GGGCCCCGTCCCCCTCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                   <  1:1162472/92‑1 (MQ=255)
                  ccGCCCTCGCCTTCGGAGCCCCTG.GGGCGCCCTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                   <  2:2043993/100‑1 (MQ=255)
                   cGCCCTCCCCTTCTGATGCCCTC.GGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTa                                                                                  <  2:2466269/101‑1 (MQ=255)
                    ccccTCCCCTTCTG.TGCCCCCGGGGCCCCGCCCCCGCCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                 <  1:4381857/100‑1 (MQ=255)
                      ccccACCTTCTGACCCCGTC.GGCAGCAGCCCCCCGCCCCGCCGGCGGGGGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                               <  2:1310461/98‑1 (MQ=255)
                      ccTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                               >  1:3207261/1‑101 (MQ=255)
                      ccTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                               >  1:964844/1‑101 (MQ=255)
                        tCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGACGCAAGGGGCCTCGGAGAGCt                                                                             >  2:1606494/1‑101 (MQ=255)
                          cccTTTTGATGCCCTC.GGGCGCCGCCACCGTCGCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcg                                                                           <  2:3340836/100‑1 (MQ=255)
                          aCCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTGGTAGAGCtct                                                                           >  1:3122024/1‑100 (MQ=255)
                              tCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                       >  2:854187/1‑101 (MQ=255)
                               cTGATCCCCGC.GGGCGCCGTCCCCGCCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGAt                                                                      <  2:3007678/101‑1 (MQ=255)
                                tgtcgCCCTG.GGGCGCCGCCCCCGCCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATg                                                                     <  1:2382822/97‑1 (MQ=255)
                                 gATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                    >  1:2893162/1‑101 (MQ=255)
                                 gATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                    >  1:1206191/1‑101 (MQ=255)
                                  atccccTC.GGGCGCCGCCCCCGCCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                   <  1:1561273/98‑1 (MQ=255)
                                  aTGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                   >  2:812000/1‑101 (MQ=255)
                                   tGCCCTC.GGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGTAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCa                                                                  <  1:1985185/101‑1 (MQ=255)
                                        tC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                             >  1:818782/1‑101 (MQ=255)
                                        gc.GGGCGCCGCCCCCTCCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                             <  2:4284514/100‑1 (MQ=255)
                                        cc.GGGCGCCGCCCCCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                             <  2:3030637/100‑1 (MQ=255)
                                         c.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggg                                                            >  1:2195231/1‑101 (MQ=255)
                                         c.GGGCGCCGCCCCCGCCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggg                                                            <  2:3427718/101‑1 (MQ=255)
                                            ggCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggc                                                          >  1:278457/1‑101 (MQ=255)
                                                 cgtcaccgtcacCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcggggc                                                     >  1:3621035/1‑101 (MQ=255)
                                                   cccccgcccccgcccgcGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCg                                                   <  1:1810231/92‑1 (MQ=255)
                                                        gccacccCCCGGGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                              <  1:1606600/99‑1 (MQ=255)
                                                            ccgcccgcCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGa                                          >  2:4342121/1‑101 (MQ=255)
                                                            ccccccgccGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGa                                          <  2:189354/98‑1 (MQ=255)
                                                             cccccgcgGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGAt                                         <  2:3458913/99‑1 (MQ=255)
                                                              gcccgccGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATg                                        >  2:3768417/1‑101 (MQ=255)
                                                               cccgccGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                       <  1:3585593/101‑1 (MQ=255)
                                                                ccgccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggc                                      >  2:1853125/1‑101 (MQ=255)
                                                                ccgccgGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggc                                      <  1:606248/101‑1 (MQ=255)
                                                                 cggggggGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcg                                     <  1:802769/97‑1 (MQ=255)
                                                                   ccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggc                                   >  1:1500340/1‑101 (MQ=255)
                                                                    cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                  >  2:407313/1‑101 (MQ=255)
                                                                    cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                  >  2:3875971/1‑101 (MQ=255)
                                                                       gcggaAAGCCGCGAAAGTGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGgcggcggcg                               <  2:4014913/101‑1 (MQ=255)
                                                                         ggaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCt                             <  2:3207032/98‑1 (MQ=255)
                                                                          gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                            >  1:4315543/1‑101 (MQ=255)
                                                                          gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                            >  2:2707399/1‑101 (MQ=255)
                                                                          gaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                            <  2:1549979/99‑1 (MQ=255)
                                                                            aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                          >  1:298235/1‑101 (MQ=255)
                                                                                 gcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggc                     <  2:510376/101‑1 (MQ=255)
                                                                                  cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                    >  1:3893809/1‑101 (MQ=255)
                                                                                  cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                    <  2:3982982/101‑1 (MQ=255)
                                                                                   gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                   >  1:78607/1‑101 (MQ=255)
                                                                                    aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                  >  2:1710356/1‑101 (MQ=255)
                                                                                    aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                  <  1:1236401/101‑1 (MQ=255)
                                                                                    aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCCCCGggcggc                  >  2:3059489/1‑101 (MQ=255)
                                                                                     aaGGGCAGGCTCATGAGCGATGCAACGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGTCGCTCGCCGGGTGGCt                 >  1:728634/1‑101 (MQ=255)
                                                                                      aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCCTCTGGGGCGGGGCCGGCACCACGATGGCGGGGGGGCTCGCCGggcggccg                >  2:942364/1‑99 (MQ=255)
                                                                                      aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                <  2:2282884/101‑1 (MQ=255)
                                                                                      aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                >  1:4052142/1‑101 (MQ=255)
                                                                                       gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa               >  2:3589505/1‑101 (MQ=255)
                                                                                       gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa               <  1:1662943/101‑1 (MQ=255)
                                                                                       ggagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGGGCCTGa               >  2:2903888/11‑101 (MQ=255)
                                                                                        gagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAt              <  1:1348671/92‑1 (MQ=255)
                                                                                         gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg             <  2:3121800/101‑1 (MQ=255)
                                                                                         gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg             <  2:2195092/101‑1 (MQ=255)
                                                                                          gaaaacaaTGACCGATGTCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt            <  2:3610242/94‑1 (MQ=255)
                                                                                           aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg           <  2:1225821/101‑1 (MQ=255)
                                                                                           aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg           <  1:854269/101‑1 (MQ=255)
                                                                                             gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGAGGGCGGCGGCGCTCGCCGGGCGGCGGATGTGGc         >  2:422364/1‑101 (MQ=255)
                                                                                               aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt       >  1:1779811/3‑101 (MQ=255)
                                                                                                cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt      >  2:417265/1‑101 (MQ=255)
                                                                                                aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt      >  2:107095/2‑101 (MQ=255)
                                                                                                aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt      >  1:1633827/2‑101 (MQ=255)
                                                                                                aaTGACCGATACCAGAGGCCTCGTCGAGGCCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt      >  1:1114087/2‑101 (MQ=255)
                                                                                                 aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc     >  1:1851874/1‑101 (MQ=255)
                                                                                                 aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc     <  2:4022155/101‑1 (MQ=255)
                                                                                                 aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc     >  2:3697695/1‑101 (MQ=255)
                                                                                                 aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc     >  1:3494697/1‑101 (MQ=255)
                                                                                                  tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg    <  1:3257311/101‑1 (MQ=255)
                                                                                                   gAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGa   <  2:1972808/101‑1 (MQ=255)
                                                                                                     |                                                                                                   
CCGCCGACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAC  >  NC_014034/2155340‑2155539

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG