Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,445 G→A 16.2% A4T (GCA→ACC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4450G→A16.2% 80.9 ‑0.5 ‑0.0209A4T (GCA→ACA) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/19):  ref base (87/88):  total (102/107)
Fisher's exact test strand distribution p-value = 5.79e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.93e-01

Only 100 of 219 total aligned reads displayed.

GACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGG  >  NC_014034/2155345‑2155544
                                                                                                     |                                                                                                   
gccccGGCCGGGGCCCCCCTCCCCTTCTGATTCCCCC.GGGCGCCGACCCCGCCCCCCCCCCCGGGGCGGACAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                     <  1:3972563/99‑1 (MQ=255)
gccccGGCCGCGGTCGCCCTCCCCTTCTGATGCCCTC.GGGCGCCGCCACCGCCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                     <  2:1001125/99‑1 (MQ=255)
gccccGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                     <  2:2596505/99‑1 (MQ=255)
gACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                     >  2:907690/1‑101 (MQ=255)
  cccGGCCGCGGTCGCCCTCCCCTTCTGATGCCCTC.GGGCGCCGCCCCCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                                   <  1:4399679/101‑1 (MQ=255)
  cccGGCCGCGGGCCCCCTCCCCTTCTGATGCCCTC.GGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                                   <  2:1752315/101‑1 (MQ=255)
   ccGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCaa                                                                                                  >  1:4181099/1‑101 (MQ=255)
   ccGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCaa                                                                                                  >  2:1649899/1‑101 (MQ=255)
   ccGGCCGCGGCCGCCCTCCCCTTCTGATCCCCTC.GGCCGCCGTCACCGCCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCaa                                                                                                  <  1:1565814/101‑1 (MQ=255)
    cGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGGTCATGAGCGGTGCagg                                                                                                 >  2:3008584/1‑99 (MQ=255)
       ccggggccccccTCCCTTTCTGATGCCCTC.GGGCGCCGCCCCCGCCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgggg                                                                                              <  2:3305715/92‑1 (MQ=255)
       ccGCGGCCCCCCTCCCCTTCTGACGCCCTG.GGGCGCCGTCCCCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgggg                                                                                              <  2:3039354/101‑1 (MQ=255)
        cgcgGCCCCCCTCCCCTTCTGACCCCCTC.GGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGc                                                                                             <  1:3421433/101‑1 (MQ=255)
         ccggccccccTCCCCTTCTGATCCCCTC.GGGCGCCGCCACCGCCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGcc                                                                                            <  2:484176/94‑1 (MQ=255)
            gTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                         >  1:3002544/1‑101 (MQ=255)
            gTCCCCCTCCCCTTCTGATCCCCTC.GGGCGCCGTCCCCGTCCCCGCCCGCCGGGGGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                         <  2:2814505/101‑1 (MQ=255)
             tccccccccccTTCTGATCCCCCC.GGGCCCCGTCCCCCTCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                        <  1:1162472/92‑1 (MQ=255)
             ccGCCCTCGCCTTCGGAGCCCCTG.GGGCGCCCTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                        <  2:2043993/100‑1 (MQ=255)
              cGCCCTCCCCTTCTGATGCCCTC.GGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTa                                                                                       <  2:2466269/101‑1 (MQ=255)
               cccccccccTTCTGATGCCCCC.GGGCCCCGTCCCCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                      <  1:266978/94‑1 (MQ=255)
               ccccTCCCCTTCTG.TGCCCCCGGGGCCCCGCCCCCGCCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                      <  1:4381857/100‑1 (MQ=255)
                cccTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGAGGCAAGGGGCCTCGTaga                                                                                     >  2:791631/1‑101 (MQ=255)
                 ccccACCTTCTGACCCCGTC.GGCAGCAGCCCCCCGCCCCGCCGGCGGGGGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                    <  2:1310461/98‑1 (MQ=255)
                 ccTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                    >  1:964844/1‑101 (MQ=255)
                 ccTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                    >  1:3207261/1‑101 (MQ=255)
                 ccTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                    >  2:385682/1‑101 (MQ=255)
                   tCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGACGCAAGGGGCCTCGGAGAGCt                                                                                  >  2:1606494/1‑101 (MQ=255)
                        ttCTGAGCCCCTG.GGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtcg                                                                             <  1:2821915/101‑1 (MQ=255)
                         tCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                            >  1:4437611/1‑101 (MQ=255)
                         tCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                            >  2:854187/1‑101 (MQ=255)
                           tgtcgCCCTG.GGGCGCCGCCCCCGCCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATg                                                                          <  1:2382822/97‑1 (MQ=255)
                            gATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                         >  1:2893162/1‑101 (MQ=255)
                            gATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                         >  1:1206191/1‑101 (MQ=255)
                             aTGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                        >  2:812000/1‑101 (MQ=255)
                                   tC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                                  >  1:818782/1‑101 (MQ=255)
                                      gggCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgggg                                                                >  1:2140914/1‑101 (MQ=255)
                                                 ccgcccccgcccgcCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTca                                                     <  1:3732939/95‑1 (MQ=255)
                                                       ccgcccgcCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGa                                               >  2:4342121/1‑101 (MQ=255)
                                                       ccccccgccGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGa                                               <  2:189354/98‑1 (MQ=255)
                                                        cccccgccGGGGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGAt                                              <  1:1812969/99‑1 (MQ=255)
                                                         gcccgccGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATg                                             >  2:3768417/1‑101 (MQ=255)
                                                          cccgcgGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                            <  2:2902759/101‑1 (MQ=255)
                                                          cccgccGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                            <  1:3585593/101‑1 (MQ=255)
                                                          ccccacgcgcggcAAGCACCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                            <  2:4014818/93‑1 (MQ=255)
                                                           ccgccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggc                                           >  2:1853125/1‑101 (MQ=255)
                                                            cggggggGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcg                                          <  1:802769/97‑1 (MQ=255)
                                                              ccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggc                                        >  1:1500340/1‑101 (MQ=255)
                                                               cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                       >  2:407313/1‑101 (MQ=255)
                                                               cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                       >  2:3443594/1‑101 (MQ=255)
                                                               cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                       >  1:1625778/1‑101 (MQ=255)
                                                                gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                      >  1:1972937/1‑101 (MQ=255)
                                                                gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                      >  1:1636649/1‑101 (MQ=255)
                                                                     gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                 >  2:2707399/1‑101 (MQ=255)
                                                                     gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                 >  1:4315543/1‑101 (MQ=255)
                                                                     gaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                 <  2:1549979/99‑1 (MQ=255)
                                                                       aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                               >  1:298235/1‑101 (MQ=255)
                                                                            gcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggc                          <  2:510376/101‑1 (MQ=255)
                                                                             cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                         >  2:4258425/1‑101 (MQ=255)
                                                                             cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                         >  1:3893809/1‑101 (MQ=255)
                                                                              gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                        >  1:78607/1‑101 (MQ=255)
                                                                               aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                       >  2:1710356/1‑101 (MQ=255)
                                                                               aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                       <  2:3002323/101‑1 (MQ=255)
                                                                               aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                       <  1:1236401/101‑1 (MQ=255)
                                                                                aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                      <  1:2758118/101‑1 (MQ=255)
                                                                                 aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCCTCTGGGGCGGGGCCGGCACCACGATGGCGGGGGGGCTCGCCGggcggccg                     >  2:942364/1‑99 (MQ=255)
                                                                                 aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                     <  2:2282884/101‑1 (MQ=255)
                                                                                 aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                     <  1:623394/101‑1 (MQ=255)
                                                                                 aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                     >  1:4052142/1‑101 (MQ=255)
                                                                                  gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGCCGGGCGGCTGa                    >  2:3112701/1‑101 (MQ=255)
                                                                                   gagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAt                   <  1:1348671/92‑1 (MQ=255)
                                                                                    gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                  <  2:2195092/101‑1 (MQ=255)
                                                                                     gaaaacaaTGACCGATGTCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt                 <  2:3610242/94‑1 (MQ=255)
                                                                                     gaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt                 <  2:418279/94‑1 (MQ=255)
                                                                                      aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg                <  2:1225821/101‑1 (MQ=255)
                                                                                      aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg                <  1:854269/101‑1 (MQ=255)
                                                                                        gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGGTGATGTGGc              >  2:3579518/1‑101 (MQ=255)
                                                                                         aaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCa             <  2:2498359/98‑1 (MQ=255)
                                                                                         aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa             >  1:1943500/1‑101 (MQ=255)
                                                                                           cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt           <  2:3400019/101‑1 (MQ=255)
                                                                                           aaTGACCGATACCAGAGGCCTCGTCGAGGCCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt           >  1:1114087/2‑101 (MQ=255)
                                                                                            aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc          <  1:2784472/101‑1 (MQ=255)
                                                                                            aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc          <  2:4022155/101‑1 (MQ=255)
                                                                                            aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc          >  2:3697695/1‑101 (MQ=255)
                                                                                            aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc          >  1:3494697/1‑101 (MQ=255)
                                                                                             tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg         <  2:3932942/101‑1 (MQ=255)
                                                                                             tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg         <  2:416003/101‑1 (MQ=255)
                                                                                             tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg         <  1:3257311/101‑1 (MQ=255)
                                                                                             tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg         <  1:591519/101‑1 (MQ=255)
                                                                                              caccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGa        <  2:4361741/98‑1 (MQ=255)
                                                                                               accGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAc       <  1:3118320/99‑1 (MQ=255)
                                                                                               aGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAc       >  1:2005210/1‑101 (MQ=255)
                                                                                                ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc      >  1:3058629/2‑101 (MQ=255)
                                                                                                 cGATACCAGAGGCTTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg     >  2:3263774/1‑101 (MQ=255)
                                                                                                 cGATACCAGAGGCCTCGTCTAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg     <  2:3144636/101‑1 (MQ=255)
                                                                                                 cGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg     <  1:1741035/101‑1 (MQ=255)
                                                                                                 cGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg     <  2:2758640/101‑1 (MQ=255)
                                                                                                  gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa    <  1:2485620/101‑1 (MQ=255)
                                                                                                  gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGACGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa    >  1:1775999/1‑101 (MQ=255)
                                                                                                   aTGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAg   >  2:737552/1‑101 (MQ=255)
                                                                                                    tGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAgg  <  2:1986887/101‑1 (MQ=255)
                                                                                                     |                                                                                                   
GACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGG  >  NC_014034/2155345‑2155544

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG