Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,447 A→C 17.3% A4A (GCA→ACC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4470A→C17.3% 103.9 ‑0.8 ‑0.1208A4A (GCA→GCC) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/21):  ref base (86/86):  total (101/107)
Fisher's exact test strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.14e-01

Only 100 of 222 total aligned reads displayed.

CCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGC  >  NC_014034/2155347‑2155547
                                                                                                     |                                                                                                    
cccGGCCGCGGTCGCCCTCCCCTTCTGATGCCCTC.GGGCGCCGCCCCCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                                      <  1:4399679/101‑1 (MQ=255)
  cGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGGTCATGAGCGGTGCagg                                                                                                    >  2:3008584/1‑99 (MQ=255)
   ggCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgg                                                                                                   >  1:2104105/1‑101 (MQ=255)
     ccggggccccccTCCCTTTCTGATGCCCTC.GGGCGCCGCCCCCGCCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgggg                                                                                                 <  2:3305715/92‑1 (MQ=255)
     ccGCGGCCGCCCTCCCCTTCTGAGGCCCGC.GGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgggg                                                                                                 <  2:2570500/101‑1 (MQ=255)
     ccGCGGCCCCCCTCCCCTTCTGACGCCCTG.GGGCGCCGTCCCCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgggg                                                                                                 <  2:3039354/101‑1 (MQ=255)
      cgcgGCCCCCCTCCCCTTCTGACCCCCTC.GGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGc                                                                                                <  1:3421433/101‑1 (MQ=255)
       ccggccccccTCCCCTTCTGATCCCCTC.GGGCGCCGCCACCGCCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGcc                                                                                               <  2:484176/94‑1 (MQ=255)
          gTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                            >  1:3002544/1‑101 (MQ=255)
          gTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                            >  2:788239/1‑101 (MQ=255)
          gTCCCCCTCCCCTTCTGATCCCCTC.GGGCGCCGTCCCCGTCCCCGCCCGCCGGGGGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                            <  2:2814505/101‑1 (MQ=255)
          gTCCCCCTCCCCTTCTGATCCCCCC.GGGCGCCGTCACCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                            <  1:2645760/101‑1 (MQ=255)
           tccccccccccTTCTGATCCCCCC.GGGCCCCGTCCCCCTCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                           <  1:1162472/92‑1 (MQ=255)
           ccGCCCTCGCCTTCGGAGCCCCTG.GGGCGCCCTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                           <  2:2043993/100‑1 (MQ=255)
            cccccTCCCCTTCGGATGCCCCC.GGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTa                                                                                          <  2:4055121/99‑1 (MQ=255)
             ccccTCCCCTTCTG.TGCCCCCGGGGCCCCGCCCCCGCCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                         <  1:4381857/100‑1 (MQ=255)
              cccTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                        >  2:2485463/1‑101 (MQ=255)
                 tCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGACGCAAGGGGCCTCGGAGAGCt                                                                                     >  2:1606494/1‑101 (MQ=255)
                     cTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtc                                                                                 >  1:416043/1‑101 (MQ=255)
                      ttCTGAGCCCCTG.GGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtcg                                                                                <  1:2821915/101‑1 (MQ=255)
                       tCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                               >  1:4437611/1‑101 (MQ=255)
                       tCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                               >  2:854187/1‑101 (MQ=255)
                          gATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                            >  1:1206191/1‑101 (MQ=255)
                           atccccTC.GGGCGCCGCCCCCGCCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                           <  1:1561273/98‑1 (MQ=255)
                           aTGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                           >  2:812000/1‑101 (MQ=255)
                                 tC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                                     >  1:818782/1‑101 (MQ=255)
                                  c.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggg                                                                    >  1:2195231/1‑101 (MQ=255)
                                    gggCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgggg                                                                   >  1:4022412/1‑101 (MQ=255)
                                               ccgcccccgcccgcCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTca                                                        <  1:3732939/95‑1 (MQ=255)
                                                 gtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                      >  2:4016729/1‑101 (MQ=255)
                                                      cccccgccGGGGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGAt                                                 <  1:1812969/99‑1 (MQ=255)
                                                       gcccgccGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATg                                                >  2:3768417/1‑101 (MQ=255)
                                                        cccgcgGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                               <  2:2902759/101‑1 (MQ=255)
                                                        cccgccGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                               <  1:3585593/101‑1 (MQ=255)
                                                        ccccacgcgcggcAAGCACCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                               <  2:4014818/93‑1 (MQ=255)
                                                         ccgccgGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggc                                              <  1:606248/101‑1 (MQ=255)
                                                          cggggggGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcg                                             <  1:802769/97‑1 (MQ=255)
                                                             cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                          >  2:3443594/1‑101 (MQ=255)
                                                             cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                          >  2:3875971/1‑101 (MQ=255)
                                                             cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                          >  1:1625778/1‑101 (MQ=255)
                                                              gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                         >  2:194524/1‑101 (MQ=255)
                                                              gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                         >  1:1972937/1‑101 (MQ=255)
                                                              gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                         >  1:1636649/1‑101 (MQ=255)
                                                               ggcgacAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcggc                                        <  2:2639329/101‑1 (MQ=255)
                                                                 cggcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGgcgc                                      >  2:2230985/1‑101 (MQ=255)
                                                                   gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                    >  1:4315543/1‑101 (MQ=255)
                                                                   gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                    >  2:2707399/1‑101 (MQ=255)
                                                                   gaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                    <  2:1549979/99‑1 (MQ=255)
                                                                    cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTcc                                   >  1:3176241/1‑100 (MQ=255)
                                                                    aaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                                   <  2:3176954/100‑1 (MQ=255)
                                                                     aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                                  >  1:298235/1‑101 (MQ=255)
                                                                         cgcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCggg                              >  1:722885/1‑101 (MQ=255)
                                                                          gcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggc                             <  2:510376/101‑1 (MQ=255)
                                                                           cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                            >  1:3893809/1‑101 (MQ=255)
                                                                           cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                            >  2:4258425/1‑101 (MQ=255)
                                                                            gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                           >  1:78607/1‑101 (MQ=255)
                                                                             aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                          <  2:3002323/101‑1 (MQ=255)
                                                                             aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                          >  2:1710356/1‑101 (MQ=255)
                                                                             aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                          <  1:1236401/101‑1 (MQ=255)
                                                                              aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                         <  1:2758118/101‑1 (MQ=255)
                                                                               aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCCTCTGGGGCGGGGCCGGCACCACGATGGCGGGGGGGCTCGCCGggcggccg                        >  2:942364/1‑99 (MQ=255)
                                                                                gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGCCGGGCGGCTGa                       >  2:3112701/1‑101 (MQ=255)
                                                                                gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa                       >  2:3589505/1‑101 (MQ=255)
                                                                                gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa                       <  1:907775/101‑1 (MQ=255)
                                                                                 gagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAt                      <  1:1348671/92‑1 (MQ=255)
                                                                                 gagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAt                      <  2:1784988/92‑1 (MQ=255)
                                                                                  gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGTGCTCGTCGATGCCATCTGGGGCGGTGCCGTCACAAAGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                     >  2:1239165/1‑101 (MQ=255)
                                                                                   gaaaacaaTGACCGATGTCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt                    <  2:3610242/94‑1 (MQ=255)
                                                                                   gaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt                    <  2:418279/94‑1 (MQ=255)
                                                                                     aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCCGATGTgg                  >  2:4296659/6‑101 (MQ=255)
                                                                                       aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa                >  1:1943500/1‑101 (MQ=255)
                                                                                        aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGTCAt               <  2:1670473/99‑1 (MQ=255)
                                                                                        aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt               >  1:1779811/3‑101 (MQ=255)
                                                                                         cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt              <  2:3400019/101‑1 (MQ=255)
                                                                                         aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt              >  2:107095/2‑101 (MQ=255)
                                                                                         aaTGACCGATACCAGAGGCCTCGTCGAGGCCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt              >  1:1114087/2‑101 (MQ=255)
                                                                                          aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc             >  2:3697695/1‑101 (MQ=255)
                                                                                          aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc             <  1:2784472/101‑1 (MQ=255)
                                                                                          aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc             >  1:3494697/1‑101 (MQ=255)
                                                                                          aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc             <  1:2464081/101‑1 (MQ=255)
                                                                                           tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg            <  2:3932942/101‑1 (MQ=255)
                                                                                           tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg            <  2:416003/101‑1 (MQ=255)
                                                                                           tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg            <  1:3257311/101‑1 (MQ=255)
                                                                                           tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg            <  2:1863892/101‑1 (MQ=255)
                                                                                            caccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGa           <  2:4361741/98‑1 (MQ=255)
                                                                                             accGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAc          <  1:3118320/99‑1 (MQ=255)
                                                                                              ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc         >  1:3058629/2‑101 (MQ=255)
                                                                                              ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc         <  1:2874386/100‑1 (MQ=255)
                                                                                              ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc         <  1:4198433/100‑1 (MQ=255)
                                                                                              ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc         >  1:967410/2‑101 (MQ=255)
                                                                                               cGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGCCGGGCGGCTGATGTGGCATTCGACCg        >  2:2339364/1‑101 (MQ=255)
                                                                                               cGATACCAGAGGCTTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg        >  2:3263774/1‑101 (MQ=255)
                                                                                               cGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg        <  2:2758640/101‑1 (MQ=255)
                                                                                               cGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg        <  1:1741035/101‑1 (MQ=255)
                                                                                                 aTGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAg      <  2:1636543/101‑1 (MQ=255)
                                                                                                 aTGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAg      >  2:737552/1‑101 (MQ=255)
                                                                                                  tGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAgg     <  2:1986887/101‑1 (MQ=255)
                                                                                                    cAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTg   <  1:1577283/101‑1 (MQ=255)
                                                                                                    cAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTg   <  2:895164/101‑1 (MQ=255)
                                                                                                     aaGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGc  >  1:2298305/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
CCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGC  >  NC_014034/2155347‑2155547

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG