Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,450 G→A 17.3% R5R (AGG→AGA)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4500G→A17.3% 94.3 ‑1.0 ‑0.0208R5R (AGG→AGA) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/21):  ref base (89/83):  total (104/104)
Fisher's exact test strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 218 total aligned reads displayed.

GGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGG  >  NC_014034/2155350‑2155550
                                                                                                     |                                                                                                    
ggCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAgg                                                                                                      >  1:2104105/1‑101 (MQ=255)
 gCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAggg                                                                                                     >  1:4431665/1‑101 (MQ=255)
    ccggccccccTCCCCTTCTGATCCCCTC.GGGCGCCGCCACCGCCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGcc                                                                                                  <  2:484176/94‑1 (MQ=255)
      ggTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                                >  1:2969635/1‑101 (MQ=255)
       gTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                               >  1:50067/1‑101 (MQ=255)
       gTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                               >  1:3002544/1‑101 (MQ=255)
        tccccccccccTTCTGATCCCCCC.GGGCCCCGTCCCCCTCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGt                                                                                              <  1:1162472/92‑1 (MQ=255)
         cccccTCCCCTTCTGATCCCCTC.GGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTa                                                                                             <  1:346486/99‑1 (MQ=255)
         cccccTCCCCTTCGGATGCCCCC.GGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTa                                                                                             <  2:4055121/99‑1 (MQ=255)
          cccccccccTTCTGATGCCCCC.GGGCCCCGTCCCCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                            <  1:266978/94‑1 (MQ=255)
          ccccTCCCCTTCTG.TGCCCCCGGGGCCCCGCCCCCGCCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                            <  1:4381857/100‑1 (MQ=255)
           cccTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                           >  2:2485463/1‑101 (MQ=255)
           cccTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGAGGCAAGGGGCCTCGTaga                                                                                           >  2:791631/1‑101 (MQ=255)
            ccTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                          >  2:385682/1‑101 (MQ=255)
            ccTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                          >  1:3207261/1‑101 (MQ=255)
            ccTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                          >  1:964844/1‑101 (MQ=255)
              tCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCt                                                                                        >  2:2010509/1‑101 (MQ=255)
               cACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtc                                                                                       >  2:2150208/1‑101 (MQ=255)
                cccTTTTGATGCCCTC.GGGCGCCGCCACCGTCGCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcg                                                                                      <  2:3340836/100‑1 (MQ=255)
                aCCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTGGTAGAGCtct                                                                                      >  1:3122024/1‑100 (MQ=255)
                  cTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtc                                                                                    >  1:416043/1‑101 (MQ=255)
                   ttCTGAGCCCCTG.GGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtcg                                                                                   <  1:2821915/101‑1 (MQ=255)
                    tCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                                  >  2:854187/1‑101 (MQ=255)
                      tGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATg                                                                                >  1:1550084/1‑101 (MQ=255)
                       gATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                               >  1:1206191/1‑101 (MQ=255)
                        aTGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                              >  2:812000/1‑101 (MQ=255)
                         tGCCCTC.GGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGTAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCa                                                                             <  1:1985185/101‑1 (MQ=255)
                              cc.GGGCGCCGCCCCCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                                        <  2:3030637/100‑1 (MQ=255)
                               c.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggg                                                                       >  1:2195231/1‑101 (MQ=255)
                               c.GGGCGCCGCCCCCGCCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggg                                                                       <  2:3427718/101‑1 (MQ=255)
                                     gcCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcggg                                                                  >  2:3607968/1‑101 (MQ=255)
                                            ccgcccccgcccgcCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTca                                                           <  1:3732939/95‑1 (MQ=255)
                                              gtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                         >  2:4016729/1‑101 (MQ=255)
                                              gccacccCCCGGGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                         <  1:1606600/99‑1 (MQ=255)
                                                ccccccccgcgGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcaccac                                                       <  1:306494/96‑1 (MQ=255)
                                                 cccgcccgcCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                                      <  1:4224156/100‑1 (MQ=255)
                                                   cccccgcgGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGAt                                                    <  1:1764613/99‑1 (MQ=255)
                                                   cccccgcgGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGAt                                                    <  2:3458913/99‑1 (MQ=255)
                                                   cccccgccGGGGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGAt                                                    <  1:1812969/99‑1 (MQ=255)
                                                    gcccgccGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATg                                                   >  2:3768417/1‑101 (MQ=255)
                                                      ccgccgGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggc                                                 <  1:606248/101‑1 (MQ=255)
                                                          ggggGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                             <  1:3496541/100‑1 (MQ=255)
                                                           gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                            <  2:3280481/101‑1 (MQ=255)
                                                           gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                            >  2:1617985/1‑101 (MQ=255)
                                                            ggcgacAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcggc                                           <  2:2639329/101‑1 (MQ=255)
                                                              cggcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGgcgc                                         >  2:2230985/1‑101 (MQ=255)
                                                               ggaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCt                                        <  2:3207032/98‑1 (MQ=255)
                                                                gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                       >  1:4315543/1‑101 (MQ=255)
                                                                gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                       >  2:2707399/1‑101 (MQ=255)
                                                                   aGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGcc                                    <  1:788321/101‑1 (MQ=255)
                                                                      cgcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCggg                                 >  1:722885/1‑101 (MQ=255)
                                                                       gcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggc                                <  2:510376/101‑1 (MQ=255)
                                                                        cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                               <  2:3982982/101‑1 (MQ=255)
                                                                         gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                              >  2:1583224/1‑101 (MQ=255)
                                                                          aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                             >  2:1710356/1‑101 (MQ=255)
                                                                          aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                             <  2:3002323/101‑1 (MQ=255)
                                                                          aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                             <  1:1236401/101‑1 (MQ=255)
                                                                          aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCCCCGggcggc                             >  2:3059489/1‑101 (MQ=255)
                                                                            aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCCTCTGGGGCGGGGCCGGCACCACGATGGCGGGGGGGCTCGCCGggcggccg                           >  2:942364/1‑99 (MQ=255)
                                                                            aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                           <  2:3630425/101‑1 (MQ=255)
                                                                             gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCNCCNNGGCGGCGGCGCTCCCCGGGCGGCCGa                          >  1:3598218/1‑101 (MQ=255)
                                                                             gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa                          <  1:1662943/101‑1 (MQ=255)
                                                                             ggagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGGGCCTGa                          >  2:2903888/11‑101 (MQ=255)
                                                                              gagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAt                         <  2:1784988/92‑1 (MQ=255)
                                                                              gagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAt                         <  1:1348671/92‑1 (MQ=255)
                                                                               gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                        <  2:3121800/101‑1 (MQ=255)
                                                                                 aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg                      <  2:1225821/101‑1 (MQ=255)
                                                                                 aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg                      <  1:854269/101‑1 (MQ=255)
                                                                                  aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCCGATGTgg                     >  2:4296659/6‑101 (MQ=255)
                                                                                   gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGAGGGCGGCGGCGCTCGCCGGGCGGCGGATGTGGc                    >  2:422364/1‑101 (MQ=255)
                                                                                    aaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCa                   >  2:4357495/4‑101 (MQ=255)
                                                                                     aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGTCAt                  <  2:1670473/99‑1 (MQ=255)
                                                                                     aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt                  >  1:1779811/3‑101 (MQ=255)
                                                                                      cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt                 >  2:417265/1‑101 (MQ=255)
                                                                                      cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt                 >  2:415497/1‑101 (MQ=255)
                                                                                      antGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt                 >  2:2579376/3‑101 (MQ=255)
                                                                                      aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt                 >  1:1633827/2‑101 (MQ=255)
                                                                                      aaTGACCGATACCAGAGGCCTCGTCGAGGCCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt                 >  1:1114087/2‑101 (MQ=255)
                                                                                       aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc                <  1:2784472/101‑1 (MQ=255)
                                                                                        tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg               <  1:816415/101‑1 (MQ=255)
                                                                                        tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg               <  1:3257311/101‑1 (MQ=255)
                                                                                        tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg               <  2:1863892/101‑1 (MQ=255)
                                                                                        tGACCGATACCAGAGGCCTCGTCGAGGTCAACGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg               >  1:3971944/1‑101 (MQ=255)
                                                                                         gaccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGa              <  1:3880653/98‑1 (MQ=255)
                                                                                         gaccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGa              <  1:996914/98‑1 (MQ=255)
                                                                                         gAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGa              <  2:1972808/101‑1 (MQ=255)
                                                                                           ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc            <  1:2874386/100‑1 (MQ=255)
                                                                                            cGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGCCGGGCGGCTGATGTGGCATTCGACCg           >  2:2339364/1‑101 (MQ=255)
                                                                                            cGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCg           <  1:194551/101‑1 (MQ=255)
                                                                                            cGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCg           <  2:278422/101‑1 (MQ=255)
                                                                                            cGATGCAAGGGGCATCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCCTTCGACCg           >  2:4382483/1‑101 (MQ=255)
                                                                                            cGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg           <  2:2758640/101‑1 (MQ=255)
                                                                                             gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa          <  1:2485620/101‑1 (MQ=255)
                                                                                              aTGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAg         >  2:737552/1‑101 (MQ=255)
                                                                                                 cAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTg      >  2:3910455/1‑101 (MQ=255)
                                                                                                 cAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTg      <  2:895164/101‑1 (MQ=255)
                                                                                                  aaGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGc     >  1:2298305/1‑101 (MQ=255)
                                                                                                   aGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCg    <  1:736900/101‑1 (MQ=255)
                                                                                                    ggggCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCgg   <  2:1273688/101‑1 (MQ=255)
                                                                                                    ggggCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCgg   >  2:286931/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GGCCGCGGTCGCCCTCACCTTCTGATGCCCTC.GGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGG  >  NC_014034/2155350‑2155550

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG