BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,155,463	C→G	28.8%	L10V (CTC→GTC)	RCAP_rcc02000 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,155,463	0	C→G	28.8%	186.0 ‑1.0 ‑0.2	229	L10V (CTC→GTC)	RCAP_rcc02000	hypothetical protein
Reads supporting (aligned to +/- strand): new base (31/35): ref base (88/75): total (119/110)
Fisher's exact test strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.71e-01

Only 100 of 234 total aligned reads displayed.


TCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCG  >  NC_014034/2155364‑2155563


 cACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtc                                                                                                    >  2:2150208/1‑101 (MQ=255)


  cccTTTTGATGCCCTCGGGCGCCGCCACCGTCGCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcg                                                                                                   <  2:3340836/100‑1 (MQ=255)


  aCCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTGGTAGAGCtct                                                                                                   >  1:3122024/1‑100 (MQ=255)


  aCCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcg                                                                                                   >  2:2907410/1‑101 (MQ=255)


    cTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtc                                                                                                 >  1:416043/1‑101 (MQ=255)


      tCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                                               >  2:854187/1‑101 (MQ=255)


       cTGATCCCCGCGGGCGCCGTCCCCGCCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGAt                                                                                              <  2:3007678/101‑1 (MQ=255)


          aTGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                                           >  2:812000/1‑101 (MQ=255)


                ccGGGCCCCGTCCCCGCCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                                                     <  1:4327806/100‑1 (MQ=255)


                 cGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggg                                                                                    >  1:2195231/1‑101 (MQ=255)


                        cgtcaccgtcacCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcggggc                                                                             >  1:3621035/1‑101 (MQ=255)


                          cccccgcccccgcccgcGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCg                                                                           <  1:1810231/92‑1 (MQ=255)


                           ccccgcccccgcccgcCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGt                                                                          <  2:50054/93‑1 (MQ=255)


                           caccgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCgc                                                                          >  1:826113/1‑100 (MQ=255)


                           caccgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGt                                                                          >  1:1136534/1‑101 (MQ=255)


                               gtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                                                      >  2:4016729/1‑101 (MQ=255)


                                  cccgcccgcCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACg                                                                   <  1:4224156/100‑1 (MQ=255)


                                     gcccgccGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATg                                                                >  2:3768417/1‑101 (MQ=255)


                                      ccccacgcgcggcAAGCACCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                                               <  2:4014818/93‑1 (MQ=255)


                                       ccgccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggc                                                              >  2:1853125/1‑101 (MQ=255)


                                          ccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggc                                                           >  1:1500340/1‑101 (MQ=255)


                                           cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                                          >  2:407313/1‑101 (MQ=255)


                                           cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                                          >  2:3875971/1‑101 (MQ=255)


                                           cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                                          >  2:3443594/1‑101 (MQ=255)


                                            gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                                         >  1:1972937/1‑101 (MQ=255)


                                            gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                                         >  2:1617985/1‑101 (MQ=255)


                                             ggcgacAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcggc                                                        <  2:2639329/101‑1 (MQ=255)


                                                 gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                                    >  2:2707399/1‑101 (MQ=255)


                                                 gaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                                    <  2:1549979/99‑1 (MQ=255)


                                                  cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTcc                                                   >  1:3176241/1‑100 (MQ=255)


                                                  aaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCg                                                   <  2:3176954/100‑1 (MQ=255)


                                                   aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                                                  >  1:298235/1‑101 (MQ=255)


                                                    aGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGcc                                                 <  1:788321/101‑1 (MQ=255)


                                                      ccGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCgg                                               >  2:934429/1‑101 (MQ=255)


                                                       cgcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCggg                                              <  1:117665/101‑1 (MQ=255)


                                                         cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                                            >  2:4258425/1‑101 (MQ=255)


                                                            aaGGGCAGGCTCATGAGCGATGCAACGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGTCGCTCGCCGGGTGGCt                                         >  1:728634/1‑101 (MQ=255)


                                                             aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                                        <  2:2282884/101‑1 (MQ=255)


                                                             aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                                        <  1:623394/101‑1 (MQ=255)


                                                              gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa                                       >  2:3589505/1‑101 (MQ=255)


                                                               gagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAt                                      <  2:1784988/92‑1 (MQ=255)


                                                               gagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAt                                      <  1:1348671/92‑1 (MQ=255)


                                                                gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGTGCTCGTCGATGCCATCTGGGGCGGTGCCGTCACAAAGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                                     >  2:1239165/1‑101 (MQ=255)


                                                                gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                                     <  2:3121800/101‑1 (MQ=255)


                                                                 gaaaacaaTGACCGATGTCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt                                    <  2:3610242/94‑1 (MQ=255)


                                                                 gaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt                                    <  2:418279/94‑1 (MQ=255)


                                                                 gaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt                                    <  2:2832359/94‑1 (MQ=255)


                                                                   aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCCGATGTgg                                  >  2:4296659/6‑101 (MQ=255)


                                                                     aaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCa                                >  2:4357495/4‑101 (MQ=255)


                                                                     aaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCa                                <  2:95963/98‑1 (MQ=255)


                                                                     aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa                                >  1:1943500/1‑101 (MQ=255)


                                                                      aaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAt                               >  1:3418005/3‑101 (MQ=255)


                                                                       cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt                              >  2:417265/1‑101 (MQ=255)


                                                                       cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt                              <  2:3400019/101‑1 (MQ=255)


                                                                       aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt                              >  1:1633827/2‑101 (MQ=255)


                                                                       aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt                              >  2:107095/2‑101 (MQ=255)


                                                                        aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc                             <  1:2784472/101‑1 (MQ=255)


                                                                        aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc                             >  1:3494697/1‑101 (MQ=255)


                                                                         tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg                            <  2:3932942/101‑1 (MQ=255)


                                                                         tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg                            <  1:816415/101‑1 (MQ=255)


                                                                         tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg                            <  1:591519/101‑1 (MQ=255)


                                                                          gaccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGa                           <  1:996914/98‑1 (MQ=255)


                                                                          gaccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGa                           <  1:3880653/98‑1 (MQ=255)


                                                                          gAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGa                           <  2:1972808/101‑1 (MQ=255)


                                                                            ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc                         <  1:2874386/100‑1 (MQ=255)


                                                                            ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc                         >  1:967410/2‑101 (MQ=255)


                                                                             cgataccggaggCCTCGTCGAGGTCATCGATGCCCTTTGGGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg                        <  1:3028980/91‑1 (MQ=255)


                                                                             cGATGCAAGGGGCATCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCCTTCGACCg                        >  2:4382483/1‑101 (MQ=255)


                                                                             cGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg                        <  2:2758640/101‑1 (MQ=255)


                                                                             cGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg                        <  1:1741035/101‑1 (MQ=255)


                                                                              gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa                       <  1:2485620/101‑1 (MQ=255)


                                                                              gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGACGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa                       >  1:1775999/1‑101 (MQ=255)


                                                                                  ccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAgggg                   >  2:515703/6‑99 (MQ=255)


                                                                                  cAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTg                   <  1:1577283/101‑1 (MQ=255)


                                                                                  cAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTg                   >  2:168496/1‑101 (MQ=255)


                                                                                   cagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGc                  <  2:2003137/97‑1 (MQ=255)


                                                                                   cagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGc                  >  1:1618584/5‑101 (MQ=255)


                                                                                   cagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGc                  <  1:3543580/97‑1 (MQ=255)


                                                                                   aaGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGc                  >  1:2298305/1‑101 (MQ=255)


                                                                                    aGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGGTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCg                 >  1:3759839/1‑101 (MQ=255)


                                                                                     gaggCCTCGGCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCgg                <  2:541722/99‑1 (MQ=255)


                                                                                      aggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCggg               >  2:1870436/2‑101 (MQ=255)


                                                                                         ccTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgc            <  1:1256640/101‑1 (MQ=255)


                                                                                         ccTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgc            <  1:1997625/101‑1 (MQ=255)


                                                                                         ccTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGgcgc            >  2:3235617/1‑101 (MQ=255)


                                                                                          cTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcg           >  1:1046059/1‑101 (MQ=255)


                                                                                          cTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGgcgcg           >  2:1653397/1‑101 (MQ=255)


                                                                                           tCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcgg          <  1:2107241/101‑2 (MQ=255)


                                                                                           tCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGGGCGGgcgcgg          >  2:2896056/1‑100 (MQ=255)


                                                                                            cGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGGGCGGGCGCGCa         >  2:3039806/1‑101 (MQ=255)


                                                                                             gtcgagGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcggcg        >  1:2623276/4‑98 (MQ=255)


                                                                                             gTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCGCCAGGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCaa        <  1:4024891/101‑1 (MQ=255)


                                                                                             gTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCaa        >  1:4312618/1‑101 (MQ=255)


                                                                                              tAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAg       >  1:3984294/1‑101 (MQ=255)


                                                                                              gagagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAg       <  2:807608/100‑1 (MQ=255)


                                                                                               cgagGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcggcggc      >  1:3104067/2‑96 (MQ=255)


                                                                                               agagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgc      <  2:2797378/101‑1 (MQ=255)


                                                                                               agagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgc      >  2:2360117/1‑101 (MQ=255)


                                                                                                 agCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCCAgcgc    >  2:2564220/1‑101 (MQ=255)


                                                                                                   cTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGcgccg  >  2:3682002/1‑101 (MQ=255)


TCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCG  >  NC_014034/2155364‑2155563

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG