Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,477 C→T 33.7% I14I (ATC→CTT RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4770C→T33.7% 287.6 ‑0.7 ‑1.7261I14I (ATC→ATTRCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (51/37):  ref base (92/81):  total (143/118)
Fisher's exact test strand distribution p-value = 5.12e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.20e-02

Only 100 of 263 total aligned reads displayed.

TCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGC  >  NC_014034/2155380‑2155576
                                                                                                 |                                                                                                   
tCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                                                                  >  1:818782/1‑101 (MQ=255)
ccGGGCCCCGTCCCCGCCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                                                                  <  1:4327806/100‑1 (MQ=255)
  gggCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgggg                                                                                                >  1:2140914/1‑101 (MQ=255)
   ggCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggc                                                                                               >  1:278457/1‑101 (MQ=255)
      gcCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcggg                                                                                            >  2:3607968/1‑101 (MQ=255)
        cgtcaccgtcacCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcggggc                                                                                          >  1:3621035/1‑101 (MQ=255)
           ccccgcccccgcccgcCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGt                                                                                       <  2:50054/93‑1 (MQ=255)
           caccgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGt                                                                                       >  1:1136534/1‑101 (MQ=255)
                    cccccgcgGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGAt                                                                              <  1:1764613/99‑1 (MQ=255)
                    cccccgccGGGGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGAt                                                                              <  1:1812969/99‑1 (MQ=255)
                           cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                                                       >  2:407313/1‑101 (MQ=255)
                           cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                                                       >  2:3443594/1‑101 (MQ=255)
                           cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                                                       >  1:1625778/1‑101 (MQ=255)
                            gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                                                      >  2:194524/1‑101 (MQ=255)
                            gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                                                      <  2:3280481/101‑1 (MQ=255)
                            gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                                                      >  1:1972937/1‑101 (MQ=255)
                             ggcgacAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcggc                                                                     <  2:2639329/101‑1 (MQ=255)
                              gcggaAAGCCGCGAAAGTGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGgcggcggcg                                                                    <  2:4014913/101‑1 (MQ=255)
                                ggaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCt                                                                  <  2:3207032/98‑1 (MQ=255)
                                 gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                                                 >  1:4315543/1‑101 (MQ=255)
                                  cAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTcc                                                                >  1:3176241/1‑100 (MQ=255)
                                    aGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGcc                                                              <  1:788321/101‑1 (MQ=255)
                                      ccGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCgg                                                            >  2:934429/1‑101 (MQ=255)
                                       cgcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCggg                                                           >  1:722885/1‑101 (MQ=255)
                                       cgcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCggg                                                           <  1:117665/101‑1 (MQ=255)
                                         cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                                                         >  1:2986204/1‑101 (MQ=255)
                                           aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                                                       >  2:1710356/1‑101 (MQ=255)
                                           aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                                                       <  1:1236401/101‑1 (MQ=255)
                                           aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCCCCGggcggc                                                       >  2:3059489/1‑101 (MQ=255)
                                            aaGGGCAGGCTCATGAGCGATGCAACGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGTCGCTCGCCGGGTGGCt                                                      >  1:728634/1‑101 (MQ=255)
                                             aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                                                     >  1:4052142/1‑101 (MQ=255)
                                              gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa                                                    <  1:1662943/101‑1 (MQ=255)
                                              ggagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGGGCCTGa                                                    >  2:2903888/11‑101 (MQ=255)
                                                gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                                                  <  2:3121800/101‑1 (MQ=255)
                                                 gaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt                                                 <  2:418279/94‑1 (MQ=255)
                                                 cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgt                                                 >  2:527635/1‑101 (MQ=255)
                                                   aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCCGATGTgg                                               >  2:4296659/6‑101 (MQ=255)
                                                    gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGGTGATGTGGc                                              >  2:3579518/1‑101 (MQ=255)
                                                     aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa                                             >  1:1943500/1‑101 (MQ=255)
                                                       antGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt                                           >  2:2579376/3‑101 (MQ=255)
                                                       aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt                                           >  2:107095/2‑101 (MQ=255)
                                                        aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc                                          >  1:3494697/1‑101 (MQ=255)
                                                         tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg                                         <  2:416003/101‑1 (MQ=255)
                                                         tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg                                         <  2:3932942/101‑1 (MQ=255)
                                                         tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg                                         <  2:1863892/101‑1 (MQ=255)
                                                         tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg                                         <  1:591519/101‑1 (MQ=255)
                                                         tGACCGATACCAGAGGCCTCGTCGAGGTCAACGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg                                         >  1:3971944/1‑101 (MQ=255)
                                                          gaccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGa                                        <  1:996914/98‑1 (MQ=255)
                                                          caccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGa                                        <  2:4361741/98‑1 (MQ=255)
                                                           accGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAc                                       <  1:3118320/99‑1 (MQ=255)
                                                           accGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAc                                       <  2:3815147/99‑1 (MQ=255)
                                                           aGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAc                                       >  1:2005210/1‑101 (MQ=255)
                                                            ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc                                      >  1:967410/2‑101 (MQ=255)
                                                             cgataccggaggCCTCGTCGAGGTCATCGATGCCCTTTGGGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg                                     <  1:3028980/91‑1 (MQ=255)
                                                              gataccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGa                                    >  1:4200882/10‑101 (MQ=255)
                                                              gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa                                    <  1:2485620/101‑1 (MQ=255)
                                                               ataccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAg                                   <  1:255206/93‑1 (MQ=255)
                                                               aTGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAg                                   <  2:1636543/101‑1 (MQ=255)
                                                                taccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAgg                                  <  2:4262472/94‑1 (MQ=255)
                                                                tGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAgg                                  <  2:1986887/101‑1 (MQ=255)
                                                                  cAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTg                                <  1:1577283/101‑1 (MQ=255)
                                                                  cAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTg                                <  2:895164/101‑1 (MQ=255)
                                                                   cagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGc                               >  2:3723932/5‑101 (MQ=255)
                                                                   cagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGc                               <  1:3543580/97‑1 (MQ=255)
                                                                   aaGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGc                               >  1:2298305/1‑101 (MQ=255)
                                                                   aaGGGGCCTCGTAGAGCTAGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGc                               <  1:4244169/101‑1 (MQ=255)
                                                                    aGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCg                              <  2:4431393/101‑1 (MQ=255)
                                                                    aGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGGGCg                              >  2:2781918/1‑101 (MQ=255)
                                                                     ggggCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCgg                             <  2:1273688/101‑1 (MQ=255)
                                                                     ggggCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCgg                             >  2:286931/1‑101 (MQ=255)
                                                                       ggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgc                           >  1:3771302/1‑101 (MQ=255)
                                                                          cTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcg                        >  1:1046059/1‑101 (MQ=255)
                                                                              gagagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAg                    <  2:807608/100‑1 (MQ=255)
                                                                               agagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgc                   <  2:2797378/101‑1 (MQ=255)
                                                                               agagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgc                   >  2:2360117/1‑101 (MQ=255)
                                                                                 agCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgcgc                 >  2:3351289/1‑101 (MQ=255)
                                                                                   cTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGcgccg               <  1:1493080/101‑1 (MQ=255)
                                                                                     cgtcgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCt             >  1:3394810/1‑101 (MQ=255)
                                                                                     cgtcgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCt             <  2:1606066/101‑1 (MQ=255)
                                                                                      gtcgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCtt            <  2:1851750/101‑1 (MQ=255)
                                                                                      atcgATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGcgccgggt            >  2:556941/2‑98 (MQ=255)
                                                                                      atcgATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGtt            >  2:1187682/2‑101 (MQ=255)
                                                                                       tcgATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGcgccgggtt           >  1:2686682/1‑97 (MQ=255)
                                                                                        cgATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGTTGTCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGtttt          <  2:3674820/101‑3 (MQ=255)
                                                                                        cgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTcc          >  1:830/1‑101 (MQ=255)
                                                                                        cgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTcc          >  2:1681691/1‑101 (MQ=255)
                                                                                         gATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGcgccgggtttt         >  1:1286363/1‑95 (MQ=255)
                                                                                         gATGCCCTTTGAGGTGGCGCCATCACGACGATGGCGGCGGCGCAGGCAGGGCGCCTGATGTGGCATTCGAACGCGGGGCGGGCGCGGCGGcgccgggtcgt         >  2:981603/1‑95 (MQ=255)
                                                                                         gATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCt         <  1:4016985/101‑1 (MQ=255)
                                                                                         gATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCt         >  2:1210527/1‑101 (MQ=255)
                                                                                         gATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCt         >  2:3962849/1‑101 (MQ=255)
                                                                                          aTGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGcgccgggttttc        >  1:3722828/1‑94 (MQ=255)
                                                                                          aTGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGtttttc        >  1:3090087/1‑97 (MQ=255)
                                                                                          aTGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGtttttc        >  2:2545174/1‑97 (MQ=255)
                                                                                          aTGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTg        >  1:180652/1‑101 (MQ=255)
                                                                                          aTGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTg        <  2:2710401/101‑1 (MQ=255)
                                                                                           tGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGa       <  2:2140775/101‑1 (MQ=255)
                                                                                           tGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGa       <  2:225803/101‑1 (MQ=255)
                                                                                           tGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGa       >  2:3003947/1‑101 (MQ=255)
                                                                                               aTCTTGGGCGGGGCCGTCACCACGATGGCTTCGTCTCCCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCcg   <  2:1775880/101‑1 (MQ=255)
                                                                                                 |                                                                                                   
TCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGC  >  NC_014034/2155380‑2155576

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG