Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,681 A→C 33.8% A82A (GCA→GCC RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,6810A→C33.8% 0.0 ‑2.2 ‑1.3303A82A (GCA→GCCRCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (59/43):  ref base (96/104):  total (155/148)
Fisher's exact test strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.58e-02

Only 100 of 319 total aligned reads displayed.

TGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCG  >  NC_014034/2155581‑2155781
                                                                                                    |                                                                                                    
tgctCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCa                                                                                                      <  2:2446416/101‑1 (MQ=255)
tgctCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCa                                                                                                      <  1:3235846/101‑1 (MQ=255)
  ctctGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCCAc                                                                                                    >  2:3258299/1‑101 (MQ=255)
     tGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCt                                                                                                 >  1:4088701/1‑101 (MQ=255)
     tGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCt                                                                                                 <  1:3351526/101‑1 (MQ=255)
      gggAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTc                                                                                                >  2:2703275/1‑101 (MQ=255)
           cTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTa                                                                                           <  2:1945210/101‑1 (MQ=255)
           cTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTa                                                                                           <  2:4205369/101‑1 (MQ=255)
           cTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTa                                                                                           >  1:183885/1‑101 (MQ=255)
            tGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTac                                                                                          <  2:1860355/101‑2 (MQ=255)
            tGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTac                                                                                          >  2:1104048/1‑100 (MQ=255)
            tGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAt                                                                                          <  2:3620782/101‑1 (MQ=255)
             gCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTacc                                                                                         >  1:2847439/1‑99 (MQ=255)
             gCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTacc                                                                                         <  1:275971/101‑3 (MQ=255)
             gCCCGTCGCGGGCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCATCGCTCGCCTAtc                                                                                         <  1:2414268/101‑1 (MQ=255)
              cccGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAtct                                                                                        <  2:2798135/101‑1 (MQ=255)
               ccGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAtctc                                                                                       >  1:54594/1‑101 (MQ=255)
               ccGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAtctc                                                                                       <  1:2339514/101‑1 (MQ=255)
                ggtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCg                                                                                      >  1:2185274/11‑101 (MQ=255)
                cGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCg                                                                                      <  1:1089577/101‑1 (MQ=255)
                cGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGGGGCGGGTTATTGCGAGCTCGCCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCg                                                                                      <  1:1454334/101‑1 (MQ=255)
                 gtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCgg                                                                                     >  2:3528783/10‑101 (MQ=255)
                  tggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGc                                                                                    <  2:1585284/93‑1 (MQ=255)
                  tggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGc                                                                                    >  1:1692536/9‑101 (MQ=255)
                    gcgGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGccc                                                                                  >  1:2573044/1‑101 (MQ=255)
                    gccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGccc                                                                                  >  2:2860936/7‑101 (MQ=255)
                    gccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGccc                                                                                  <  2:3380133/95‑1 (MQ=255)
                     ccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcg                                                                                 <  1:1433835/96‑1 (MQ=255)
                      cgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgc                                                                                >  1:2086496/5‑101 (MQ=255)
                       gTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcgcg                                                                               <  1:689495/101‑1 (MQ=255)
                           gCATGGCGGTCCTCGGGGGGGGGGTGGCGGCTTATTTCGGGCTGGGGCCGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCgcggg                                                                           <  2:3971688/101‑2 (MQ=255)
                             aTGGCGATCATTGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGgcgc                                                                         <  2:78594/101‑1 (MQ=255)
                             aTGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGgcgc                                                                         >  2:1184840/1‑101 (MQ=255)
                               ggCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCgcgggagag                                                                       <  2:4109168/101‑6 (MQ=255)
                               ggCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGccc                                                                       >  2:151408/1‑101 (MQ=255)
                                 cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAg                                                                     <  2:2336739/101‑1 (MQ=255)
                                 cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAg                                                                     <  2:1785798/101‑1 (MQ=255)
                                 cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAg                                                                     <  2:1043013/101‑1 (MQ=255)
                                 cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAg                                                                     <  1:3579766/101‑1 (MQ=255)
                                 cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAg                                                                     <  1:3617026/101‑1 (MQ=255)
                                   atcatcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGt                                                                   <  1:2939455/101‑1 (MQ=255)
                                   atcatcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGt                                                                   >  2:3463976/1‑101 (MQ=255)
                                    tcatcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTg                                                                  <  1:1295113/101‑1 (MQ=255)
                                      atcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGgc                                                                <  1:2882271/101‑1 (MQ=255)
                                      atcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGgc                                                                <  2:2276936/101‑1 (MQ=255)
                                      atcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGgc                                                                >  2:2001752/1‑101 (MQ=255)
                                       tcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctg                                                               >  2:2406491/1‑98 (MQ=255)
                                        cGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGGCGGTGGCCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGccgcggggctgc                                                              >  2:3127624/1‑91 (MQ=255)
                                        cGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAggggctgc                                                              >  2:3186855/1‑95 (MQ=255)
                                         ggCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgct                                                             >  1:4221064/1‑96 (MQ=255)
                                         ggCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                                                             <  2:2910045/101‑1 (MQ=255)
                                         ggCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                                                             <  1:812082/101‑1 (MQ=255)
                                         ggCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                                                             <  1:2289039/101‑1 (MQ=255)
                                          gCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTg                                                            <  1:2703465/101‑1 (MQ=255)
                                          gCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTg                                                            <  2:4017341/101‑1 (MQ=255)
                                           cGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGa                                                           >  2:759518/1‑101 (MQ=255)
                                            gAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGat                                                          >  1:2621647/1‑100 (MQ=255)
                                            gAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAc                                                          >  1:4357828/1‑101 (MQ=255)
                                            gAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAc                                                          >  1:4149782/1‑101 (MQ=255)
                                             aGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatg                                                         >  2:596782/1‑99 (MQ=255)
                                              ggCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatga                                                        >  2:3877513/1‑98 (MQ=255)
                                              ggCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatga                                                        >  2:1264338/1‑98 (MQ=255)
                                                cGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagg                                                      >  1:1899995/1‑96 (MQ=255)
                                                cGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCgg                                                      >  2:1879612/1‑101 (MQ=255)
                                                 ggTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagga                                                     >  1:3169700/1‑95 (MQ=255)
                                                  gTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggat                                                    <  1:3264006/101‑8 (MQ=255)
                                                  gTGGCGGCTTATTTCGGGCGGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggat                                                    <  2:522999/101‑8 (MQ=255)
                                                   tGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggatc                                                   >  2:1424326/1‑93 (MQ=255)
                                                   tGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggatc                                                   >  1:723826/1‑93 (MQ=255)
                                                    ggcggcTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggatca                                                  <  1:1053112/101‑10 (MQ=255)
                                                    ggcggcTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGGGCTGCTGatgaggatca                                                  >  2:527198/1‑92 (MQ=255)
                                                     gcggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtctt                                                 <  1:3092105/101‑1 (MQ=255)
                                                     gcggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtctt                                                 <  2:717056/101‑1 (MQ=255)
                                                      cggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttc                                                >  2:2587844/1‑101 (MQ=255)
                                                       ggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttct                                               <  2:4437339/101‑1 (MQ=255)
                                                        gcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTcttctt                                              >  2:1907699/1‑101 (MQ=255)
                                                             tttCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTcaca                                         <  2:1706230/101‑1 (MQ=255)
                                                                 gAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCAcaacaa                                     <  1:3718226/101‑1 (MQ=255)
                                                                  aGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCAcaacaaa                                    >  2:3068915/1‑100 (MQ=255)
                                                                   gCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAgg                                   <  1:1232742/101‑1 (MQ=255)
                                                                    cTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGa                                  >  2:806031/1‑101 (MQ=255)
                                                                    cTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGa                                  >  2:2838931/1‑101 (MQ=255)
                                                                     tCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAg                                 >  1:970780/1‑101 (MQ=255)
                                                                       ggCCGGACCTTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTc                               <  2:2103968/101‑1 (MQ=255)
                                                                         ccGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGt                             <  1:1554198/101‑1 (MQ=255)
                                                                              ccGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGAt                        <  1:1193336/101‑1 (MQ=255)
                                                                               cGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATg                       <  1:3771995/101‑1 (MQ=255)
                                                                                gTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGAGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATga                      >  2:367567/1‑101 (MQ=255)
                                                                                  gACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgacg                    >  1:3921706/1‑101 (MQ=255)
                                                                                     caccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACa                 >  2:2991233/1‑101 (MQ=255)
                                                                                      cccGGCGTCGTCTCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAg                <  2:1136443/100‑1 (MQ=255)
                                                                                      cccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAg                <  1:3040027/100‑1 (MQ=255)
                                                                                          ttggggtcgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTg            >  2:3766770/6‑101 (MQ=255)
                                                                                           cgtcgtCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTgg           >  1:438832/1‑101 (MQ=255)
                                                                                            gtcgtcGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGGCAGACTGGc          >  2:2193798/1‑101 (MQ=255)
                                                                                               gtcgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCCGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGCCGTGAGATGACGACAGACTGGCggc       >  1:3382046/1‑100 (MQ=255)
                                                                                                tcgCAACGCTCGCCTGTCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGc      <  2:888600/101‑1 (MQ=255)
                                                                                                tcgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCgggg      >  1:1339592/1‑100 (MQ=255)
                                                                                                 cgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGcc     <  1:2500779/101‑1 (MQ=255)
                                                                                                    aaCGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGcgcg  >  2:4295377/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCG  >  NC_014034/2155581‑2155781

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG