Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,693 T→C 29.1% Y86Y (TAT→TAC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,6930T→C29.1% 227.8 ‑1.2 ‑0.2274Y86Y (TAT→TAC) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (47/33):  ref base (100/94):  total (147/127)
Fisher's exact test strand distribution p-value = 2.90e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.06e-01

Only 100 of 291 total aligned reads displayed.

TGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGG  >  NC_014034/2155593‑2155790
                                                                                                    |                                                                                                 
tGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAt                                                                                                   <  2:3620782/101‑1 (MQ=255)
 gCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCCACGCTCGCCTAtc                                                                                                  >  2:1463965/1‑101 (MQ=255)
  cccGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAtct                                                                                                 <  2:2798135/101‑1 (MQ=255)
  cccGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAtct                                                                                                 <  1:3963105/101‑1 (MQ=255)
   ccGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTAtctc                                                                                                >  1:54594/1‑101 (MQ=255)
     gtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCgg                                                                                              >  2:3528783/10‑101 (MQ=255)
     gtggccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCgg                                                                                              <  1:1368851/92‑1 (MQ=255)
     gTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCgg                                                                                              <  2:1519466/101‑1 (MQ=255)
       cgcgGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGcc                                                                                            >  2:111066/1‑101 (MQ=255)
       cgcgGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGcc                                                                                            >  2:2977100/1‑101 (MQ=255)
        gcgGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGTTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGccc                                                                                           <  1:2272636/101‑1 (MQ=255)
        gccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGccc                                                                                           <  2:3380133/95‑1 (MQ=255)
        gccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGccc                                                                                           >  2:2860936/7‑101 (MQ=255)
         cgGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcg                                                                                          <  2:1943371/101‑1 (MQ=255)
         ccgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcg                                                                                          <  1:2178923/96‑1 (MQ=255)
          cgtgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgc                                                                                         >  1:2086496/5‑101 (MQ=255)
           gTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCcgcg                                                                                        <  1:689495/101‑1 (MQ=255)
            tgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgcgc                                                                                       >  2:159126/3‑101 (MQ=255)
            tgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgcgc                                                                                       <  1:3376343/99‑1 (MQ=255)
            tgggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgcgc                                                                                       >  2:2432852/3‑101 (MQ=255)
             gggCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCcgcgcg                                                                                      >  1:1536124/2‑101 (MQ=255)
               gCATGGCGGTCCTCGGGGGGGGGGTGGCGGCTTATTTCGGGCTGGGGCCGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCgcggg                                                                                    <  2:3971688/101‑2 (MQ=255)
                cATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCgcggcg                                                                                   >  1:369967/1‑101 (MQ=255)
                 gtGGCGAGCAGCGGCGAGGCGGTTGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGgggc                                                                                  <  2:1087446/100‑1 (MQ=255)
                 aTGGCGATCATTGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGgcgc                                                                                  <  2:78594/101‑1 (MQ=255)
                  tGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCCCGCCGcc                                                                                 >  1:3208862/1‑101 (MQ=255)
                  tGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCCGCGTCGGCGCAACGCTCGCCTATCTCGGCCCGCGCAGGGcc                                                                                 >  1:1439977/1‑101 (MQ=255)
                   ggCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCCGGGccg                                                                                >  1:806826/1‑100 (MQ=255)
                   ggCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGccc                                                                                >  1:2706942/1‑101 (MQ=255)
                     cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAg                                                                              >  2:4227010/1‑101 (MQ=255)
                     cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAg                                                                              >  1:707439/1‑101 (MQ=255)
                     cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAg                                                                              <  1:3579766/101‑1 (MQ=255)
                     cGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAg                                                                              <  1:3617026/101‑1 (MQ=255)
                      gATCATCGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAgg                                                                             >  2:525803/1‑101 (MQ=255)
                       atcatcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGt                                                                            >  2:3463976/1‑101 (MQ=255)
                       atcatcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGt                                                                            <  1:2939455/101‑1 (MQ=255)
                          atcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAggggct                                                                         >  1:2462369/1‑97 (MQ=255)
                          atcGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgct                                                                         >  2:1723872/1‑99 (MQ=255)
                          atcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGgc                                                                         >  2:2001752/1‑101 (MQ=255)
                          atcGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGgc                                                                         <  1:2882271/101‑1 (MQ=255)
                            cGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGGCGGTGGCCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGccgcggggctgc                                                                       >  2:3127624/1‑91 (MQ=255)
                            cGGCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAggggctgc                                                                       >  2:3186855/1‑95 (MQ=255)
                            cGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGgcgc                                                                       >  2:117492/1‑101 (MQ=255)
                            cGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGgcgc                                                                       >  2:2288894/1‑101 (MQ=255)
                             ggCGAGGCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTgctgct                                                                      <  2:2271117/101‑6 (MQ=255)
                             ggCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCt                                                                      <  2:2910045/101‑1 (MQ=255)
                                gAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAc                                                                   >  1:4297963/1‑101 (MQ=255)
                                  ggCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatga                                                                 >  2:3877513/1‑98 (MQ=255)
                                  ggCGGTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatga                                                                 >  2:1264338/1‑98 (MQ=255)
                                  ggCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAccc                                                                 <  1:2855086/101‑1 (MQ=255)
                                  ggCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAccc                                                                 >  1:1264306/1‑101 (MQ=255)
                                  ggCGGTGGCGGCTGATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGAccc                                                                 <  2:1892700/101‑1 (MQ=255)
                                     ggTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCTTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgagga                                                              <  1:3764940/101‑7 (MQ=255)
                                      gTGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggat                                                             <  1:3264006/101‑8 (MQ=255)
                                       tGGCGGCTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggatc                                                            >  2:2355421/1‑93 (MQ=255)
                                        ggcggcTTATTTCGGGCTGGGGCGGACGGTGACCACCGGCGTCGTCGCCTCGCTCGCCTACCTCGGCCCGCGCGGGGCCGAGGTGCTGCTGatgaggatca                                                           <  1:1053112/101‑10 (MQ=255)
                                         gcggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtctt                                                          <  1:3092105/101‑1 (MQ=255)
                                         gcggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtctt                                                          <  1:89330/101‑1 (MQ=255)
                                          cggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttc                                                         >  2:2587844/1‑101 (MQ=255)
                                           ggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttct                                                        <  2:4437339/101‑1 (MQ=255)
                                           ggcTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGtcttct                                                        <  1:415537/101‑1 (MQ=255)
                                             cTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCttt                                                      >  2:3057131/1‑101 (MQ=255)
                                                 tttCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTcaca                                                  <  1:1738296/101‑1 (MQ=255)
                                                  ttCGAGCTCGGCCGGACCGTGACCGCCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaa                                                 <  2:1871309/101‑1 (MQ=255)
                                                   tCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaac                                                <  2:4051882/101‑1 (MQ=255)
                                                   tCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCacaac                                                <  2:3297078/101‑1 (MQ=255)
                                                   tCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGAGGCGCTGACCCGGGTCTTCTTTCacaac                                                >  1:4386727/1‑101 (MQ=255)
                                                      aGCTGGGCCGGACCGTGACCTCCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAg                                             <  1:1300894/101‑1 (MQ=255)
                                                        cTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGa                                           >  2:806031/1‑101 (MQ=255)
                                                        cTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGa                                           >  2:2838931/1‑101 (MQ=255)
                                                           ggCCGGACCTTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTc                                        <  2:2103968/101‑1 (MQ=255)
                                                             ccGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGt                                      <  1:1681802/101‑1 (MQ=255)
                                                             ccGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGt                                      >  2:1037662/1‑101 (MQ=255)
                                                               ggACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTga                                    <  1:2833556/101‑1 (MQ=255)
                                                                  ccGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGAt                                 <  1:1193336/101‑1 (MQ=255)
                                                                   cGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATg                                <  1:3146260/101‑1 (MQ=255)
                                                                    gTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGAGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATga                               >  2:367567/1‑101 (MQ=255)
                                                                     tGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCTCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgac                              <  2:266891/101‑1 (MQ=255)
                                                                     tGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgac                              <  1:2631514/101‑1 (MQ=255)
                                                                     tGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgac                              <  1:2564383/101‑1 (MQ=255)
                                                                       accaccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgacga                            >  2:649254/1‑101 (MQ=255)
                                                                       accaccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATgacga                            >  1:3489271/1‑101 (MQ=255)
                                                                         caccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACa                          <  1:2588015/101‑1 (MQ=255)
                                                                         caccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACa                          <  1:2388247/101‑1 (MQ=255)
                                                                          cccGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAg                         <  1:3040027/100‑1 (MQ=255)
                                                                          accGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAg                         <  1:107922/101‑1 (MQ=255)
                                                                             ggCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACt                      >  2:1811686/1‑101 (MQ=255)
                                                                             ggCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACt                      >  2:59279/1‑101 (MQ=255)
                                                                              ttggggtcgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTg                     >  2:3766770/6‑101 (MQ=255)
                                                                              gCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTg                     <  1:4094770/101‑1 (MQ=255)
                                                                                gtcgtcGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGGCAGACTGGc                   >  2:2193798/1‑101 (MQ=255)
                                                                                   gtcgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCggg                >  1:23288/1‑101 (MQ=255)
                                                                                    tcgCAACGCTCGCCTGTCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGc               <  2:888600/101‑1 (MQ=255)
                                                                                     cgCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGcc              <  1:1862762/101‑1 (MQ=255)
                                                                                      gCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCcg             <  2:4163429/101‑1 (MQ=255)
                                                                                        aaCGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGcgcg           >  2:4295377/1‑101 (MQ=255)
                                                                                          cGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCgcgcg         >  2:743333/1‑100 (MQ=255)
                                                                                               gCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGcggcgcgccgc    >  2:1406013/1‑92 (MQ=255)
                                                                                                 cTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCGCGCCGCCGCgg  >  1:443929/1‑101 (MQ=255)
                                                                                                 cTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGGCGCGAGGCCGCgg  >  1:3847585/1‑101 (MQ=255)
                                                                                                    |                                                                                                 
TGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGACCACCGGCGTCGTCGCAACGCTCGCCTATCTCGGCCCGCGCGGCGCCCAGGTGGCGCTGACCCGGGTCTTCTTTCACAACAAGGAGTCGTGAGATGACGACAGACTGGCGGGCCGCGCAGCTGCGG  >  NC_014034/2155593‑2155790

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG