Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,176,472 G→C 100% A302G (GCG→GGG)  rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,176,4720G→C100.0% 777.6 204A302G (GCG→GGG) rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (105/99):  ref base (0/0):  total (105/99)

Only 100 of 208 total aligned reads displayed.

CAGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATG.CCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCGCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTGGC  >  NC_014034/2176377‑2176569
                                                                                                |                                                                                                 
cAGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTc                                                                                               >  1:4109261/1‑101 (MQ=255)
cAGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTc                                                                                               >  2:338836/1‑101 (MQ=255)
    aTTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGaa                                                                                           >  2:3801018/1‑101 (MQ=255)
     ttGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAAt                                                                                          <  1:259350/101‑1 (MQ=255)
        tttCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCa                                                                                       <  1:4444023/101‑1 (MQ=255)
         ttCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCAc                                                                                      >  2:3566687/1‑101 (MQ=255)
         ttCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCAc                                                                                      >  2:791080/1‑101 (MQ=255)
         ttCAGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCAc                                                                                      >  2:273562/1‑101 (MQ=255)
            aGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCgg                                                                                   <  2:3755886/101‑1 (MQ=255)
            aGCGCCTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCgg                                                                                   <  1:1672304/101‑1 (MQ=255)
                ccTCTTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTc                                                                               <  1:409703/101‑1 (MQ=255)
                  tctTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGt                                                                             >  1:3884444/1‑101 (MQ=255)
                  tctTCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGt                                                                             <  1:1988600/101‑1 (MQ=255)
                     tCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGcc                                                                          >  2:2416736/1‑101 (MQ=255)
                     tCCGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGcc                                                                          >  1:1556994/1‑101 (MQ=255)
                       cGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGt                                                                        >  1:972009/1‑101 (MQ=255)
                       cGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGt                                                                        >  2:1279408/1‑101 (MQ=255)
                       cGAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGt                                                                        >  2:62053/1‑101 (MQ=255)
                        gAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTc                                                                       >  1:1375859/1‑101 (MQ=255)
                        gAGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTc                                                                       >  1:3482123/1‑101 (MQ=255)
                        gAGGTGATCAAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTc                                                                       <  1:3751326/101‑1 (MQ=255)
                         aGGTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTcc                                                                      <  1:2314402/101‑1 (MQ=255)
                           gTGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCtt                                                                    <  1:1449809/101‑1 (MQ=255)
                            tGATCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTg                                                                   >  2:3609990/1‑101 (MQ=255)
                              aTCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGc                                                                 >  2:1738829/1‑101 (MQ=255)
                              aTCGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGc                                                                 <  2:2344942/101‑1 (MQ=255)
                                cGAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCAt                                                               >  1:592612/1‑101 (MQ=255)
                                 gAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATa                                                              >  2:279986/1‑101 (MQ=255)
                                 gAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATa                                                              <  2:3481883/101‑1 (MQ=255)
                                 gAGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATa                                                              >  2:3150190/1‑101 (MQ=255)
                                  aGGGGATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAg                                                             <  1:2432202/101‑1 (MQ=255)
                                   ggggATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAgg                                                            >  1:1815313/1‑101 (MQ=255)
                                   ggggATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAgg                                                            <  1:4036945/101‑1 (MQ=255)
                                   ggggATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAgg                                                            >  2:4183383/1‑101 (MQ=255)
                                    gggATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCCCCCCGGTGTTGACGAAATCGAGCCCGTCGCAATTCACCGGCTTCGTGCCGTCCTTGGCATAggc                                                           <  1:3657226/101‑1 (MQ=255)
                                     ggATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCAGCGGCTTCGTGCCGTCCTTGGCATAggcg                                                          >  2:2025276/1‑101 (MQ=255)
                                     ggATGCCCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAggcg                                                          >  1:662235/1‑101 (MQ=255)
                                      gatgcccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAggcgg                                                         >  1:1768015/6‑101 (MQ=255)
                                      gatgcccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAggcgg                                                         >  1:3677609/6‑101 (MQ=255)
                                       atgcccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAggcggc                                                        <  2:4109001/97‑1 (MQ=255)
                                       atgcccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCAGCTTCGTGCCGTCCTTGGCATAggcggc                                                        <  1:383648/97‑1 (MQ=255)
                                        tgcccccGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGgcggcg                                                       <  2:1208076/98‑1 (MQ=255)
                                               gATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGccc                                                >  2:644952/1‑100 (MQ=255)
                                               gATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCt                                                <  2:2589363/101‑1 (MQ=255)
                                               gATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCt                                                >  1:3575061/1‑101 (MQ=255)
                                                aTCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTc                                               >  1:1598110/1‑101 (MQ=255)
                                                aTCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTc                                               <  1:4356757/101‑1 (MQ=255)
                                                 tCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCg                                              <  1:681669/101‑1 (MQ=255)
                                                   gatcTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGAt                                            <  2:3294750/98‑1 (MQ=255)
                                                    ggCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGAGGGCGATAGCCTCCATg                                           >  2:4016185/1‑101 (MQ=255)
                                                     gCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGc                                          >  2:122422/1‑101 (MQ=255)
                                                      cTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGcc                                         <  2:3119054/101‑1 (MQ=255)
                                                      cTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGGCCGAATTGACCAGCTTCGGGCCGTCCTTGGTATAGGCGGCGATGGcggaggagaa                                         >  2:3198696/1‑92 (MQ=255)
                                                       tGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCNTTGGCATAGGCGGCGCTCGCCTCGATGccc                                        >  1:494045/1‑101 (MQ=255)
                                                       tGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGccc                                        <  1:4235041/101‑1 (MQ=255)
                                                       tGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGccc                                        <  1:768297/101‑1 (MQ=255)
                                                       tGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCCCGATGccc                                        >  2:1412397/1‑101 (MQ=255)
                                                        gTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGAtagatc                                       >  1:3294982/1‑96 (MQ=255)
                                                        gTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCt                                       <  1:4406368/101‑1 (MQ=255)
                                                          cGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTg                                     <  2:3913195/101‑1 (MQ=255)
                                                          cGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTg                                     >  1:3773359/1‑101 (MQ=255)
                                                           ggTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTgg                                    >  1:3465843/1‑101 (MQ=255)
                                                           ggTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTgg                                    <  1:3367617/101‑1 (MQ=255)
                                                           ggTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTgg                                    >  2:3692968/1‑101 (MQ=255)
                                                            gTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGa                                   <  2:1598004/101‑1 (MQ=255)
                                                            gTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGa                                   >  1:82508/1‑101 (MQ=255)
                                                            gTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGa                                   >  1:3007664/1‑101 (MQ=255)
                                                            gTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGAGATCGCCTCGATGCCCTTGGa                                   <  1:2548744/101‑1 (MQ=255)
                                                             tGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGACTTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGCTCGCCTGGATGCCCTTGGAg                                  >  2:252478/1‑101 (MQ=255)
                                                             tGACCAGCATCCCCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAg                                  <  2:838641/101‑1 (MQ=255)
                                                               aCCAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGc                                >  1:3744501/1‑101 (MQ=255)
                                                                 cAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCa                              <  2:1827006/101‑1 (MQ=255)
                                                                 cAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCa                              >  1:2125587/1‑101 (MQ=255)
                                                                 cAGCGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGGGCCGCCCTTGGCATAGGCGGCGATCGCCTCGACGCCCCTGGAGGccc                              >  2:1084185/1‑100 (MQ=255)
                                                                    cGTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCa                           <  2:3046449/101‑1 (MQ=255)
                                                                     gTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAg                          <  2:4431409/101‑1 (MQ=255)
                                                                     gTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAg                          >  1:1497745/1‑101 (MQ=255)
                                                                     gTCACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAg                          <  1:1354562/101‑1 (MQ=255)
                                                                       cACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGaa                        >  2:514177/1‑101 (MQ=255)
                                                                       cACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGaa                        >  1:571153/1‑101 (MQ=255)
                                                                       cACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGaa                        <  1:1260770/101‑1 (MQ=255)
                                                                       cACCCCGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGaa                        >  1:268632/1‑101 (MQ=255)
                                                                          cccGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCg                     <  1:1721988/101‑1 (MQ=255)
                                                                           ccGGTGTTGACGAAATCGAGACCGTCGGAATTCACCGGCTTCTTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGTGTCCATTCGAAGCgg                    >  2:431675/1‑101 (MQ=255)
                                                                            cGGTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCggg                   <  1:3428647/101‑1 (MQ=255)
                                                                             ggTGTTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGa                  >  2:4284243/1‑101 (MQ=255)
                                                                               tgtTGACGAAATCGAGCCCGTCGGAATTCACTGGCTTCGTGCCGTCCTTGGCATAGGCGGGGATCGCCTCGGTGCCCTTGGAGGCCATCAGCAGCGGGAAt                >  2:3662211/1‑101 (MQ=255)
                                                                                gtTGCCGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAAtt               <  2:1306036/101‑1 (MQ=255)
                                                                                gtTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAAtt               <  1:1396213/101‑1 (MQ=255)
                                                                                gtTGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAAtt               >  2:2889652/1‑101 (MQ=255)
                                                                                  tGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGc             >  1:2022864/1‑101 (MQ=255)
                                                                                  tGACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGc             >  2:1123542/1‑101 (MQ=255)
                                                                                   gACGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCa            <  1:4692/101‑1 (MQ=255)
                                                                                     cGAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATc          <  1:2343849/101‑1 (MQ=255)
                                                                                      gAAATCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCg         <  2:4328173/101‑1 (MQ=255)
                                                                                        aaTCGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAg       <  1:1165656/101‑1 (MQ=255)
                                                                                           cGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTg    <  1:3150414/101‑1 (MQ=255)
                                                                                           cGAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTg    <  1:4388804/101‑1 (MQ=255)
                                                                                            gAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGATTTGccacggcgaag   <  2:2283405/101‑11 (MQ=255)
                                                                                            gAGCCCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTgg   >  1:3406142/1‑101 (MQ=255)
                                                                                                |                                                                                                 
CAGCATTGTTTCAGCGCCTCTTCCGAGGTGATCGAGGGGATG.CCCCGATCGGGCTGTCGGTGACCAGCGTCACCCCGGTGTTGACGAAATCGAGCGCGTCGGAATTCACCGGCTTCGTGCCGTCCTTGGCATAGGCGGCGATCGCCTCGATGCCCTTGGAGGCCATCAGAAGCGGGAATTGCATCGAGGTGGC  >  NC_014034/2176377‑2176569

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG