Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,213 G→A 100% P55L (CCC→CTC)  rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2130G→A100.0% 887.0 228P55L (CCC→CTC) rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (100/128):  ref base (0/0):  total (100/128)

Only 100 of 238 total aligned reads displayed.

GCGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGT  >  NC_014034/2177115‑2177305
                                                                                                  |                                                                                                 
 cGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCa                                                                                                <  1:1647876/101‑1 (MQ=255)
  gTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAc                                                                                               <  1:4156949/101‑1 (MQ=255)
  gTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAc                                                                                               >  1:4306426/1‑101 (MQ=255)
  gTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAc                                                                                               <  1:1744045/101‑1 (MQ=255)
  gTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAc                                                                                               >  1:980208/1‑101 (MQ=255)
    gATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCa                                                                                             <  1:2218623/101‑1 (MQ=255)
    gATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCa                                                                                             <  1:1860497/101‑1 (MQ=255)
    gATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCa                                                                                             >  2:1177498/1‑101 (MQ=255)
     aTCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAg                                                                                            >  1:1930998/1‑101 (MQ=255)
      tCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGc                                                                                           <  2:2630689/101‑1 (MQ=255)
      tCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGc                                                                                           >  2:1687053/1‑101 (MQ=255)
        aaaaTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGccc                                                                                         <  2:1425778/101‑1 (MQ=255)
          aaTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCa                                                                                       >  2:3758395/1‑101 (MQ=255)
          aaTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCa                                                                                       >  2:2404159/1‑101 (MQ=255)
            tCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtc                                                                                     <  1:479721/101‑1 (MQ=255)
             ccccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtct                                                                                    >  2:2139762/1‑101 (MQ=255)
             ccccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtct                                                                                    >  1:2702282/1‑101 (MQ=255)
              cccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtctc                                                                                   >  2:1380097/1‑101 (MQ=255)
                 tttGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGt                                                                                <  1:1685065/101‑1 (MQ=255)
                  ttGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTc                                                                               >  2:1409685/1‑101 (MQ=255)
                  ttGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTc                                                                               >  1:1254736/1‑101 (MQ=255)
                    gCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGc                                                                             >  2:2861143/1‑101 (MQ=255)
                    gCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGc                                                                             <  2:47373/101‑1 (MQ=255)
                     ccccGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGcc                                                                            <  2:2564426/101‑1 (MQ=255)
                        cgcccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCttt                                                                         <  2:2510096/101‑1 (MQ=255)
                        cgcccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCttt                                                                         <  1:1205294/101‑1 (MQ=255)
                         gcccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTg                                                                        >  2:636226/1‑101 (MQ=255)
                           ccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGcc                                                                      >  1:2353898/1‑101 (MQ=255)
                           ccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGcc                                                                      <  1:1177589/101‑1 (MQ=255)
                               gATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAg                                                                  >  1:2467896/1‑101 (MQ=255)
                                aTGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGc                                                                 >  1:2901812/1‑101 (MQ=255)
                                aTGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGc                                                                 <  2:4253603/101‑1 (MQ=255)
                                aTGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGc                                                                 <  2:3352424/101‑1 (MQ=255)
                                   cAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAcc                                                              >  1:3780298/1‑101 (MQ=255)
                                    aGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAcct                                                             <  1:661100/101‑1 (MQ=255)
                                      cTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAccttc                                                           <  1:3912373/101‑1 (MQ=255)
                                      cTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAccttc                                                           <  1:169605/101‑1 (MQ=255)
                                           gACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCa                                                      <  2:1698683/101‑1 (MQ=255)
                                            aCCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCAt                                                     <  2:4097376/101‑1 (MQ=255)
                                              cgccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCt                                                   <  1:2139071/101‑1 (MQ=255)
                                              cgccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCt                                                   <  1:4011601/101‑1 (MQ=255)
                                              cgccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCt                                                   <  2:2444595/101‑1 (MQ=255)
                                               gccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCtt                                                  >  1:100741/1‑101 (MQ=255)
                                               gccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCtt                                                  >  2:2721915/1‑101 (MQ=255)
                                               gccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCCGGGTCGCCTTTGCCGAAGCACCTTCCTTCATCtt                                                  >  1:3827035/1‑101 (MQ=255)
                                                ccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTg                                                 <  2:2369143/101‑1 (MQ=255)
                                                ccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTg                                                 <  1:2110301/101‑1 (MQ=255)
                                                ccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTg                                                 <  1:2359856/101‑1 (MQ=255)
                                                ccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTg                                                 >  2:3518922/1‑101 (MQ=255)
                                                ccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTg                                                 <  2:1984270/101‑1 (MQ=255)
                                                ccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTg                                                 <  1:1413779/101‑1 (MQ=255)
                                                  gcgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAATCACCTTCCTTCATCTTGAc                                               >  1:2805919/1‑101 (MQ=255)
                                                  gcgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAc                                               >  1:3463733/1‑101 (MQ=255)
                                                  gcgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTAGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAc                                               >  2:2668848/1‑101 (MQ=255)
                                                   cgATCTGCGTTTCCGCATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACg                                              <  2:74152/101‑1 (MQ=255)
                                                   cgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACg                                              <  2:3627285/101‑1 (MQ=255)
                                                   cgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACg                                              >  1:2261130/1‑101 (MQ=255)
                                                   cgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACg                                              <  2:3435003/101‑1 (MQ=255)
                                                   cgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACg                                              >  2:1022056/1‑101 (MQ=255)
                                                    gATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGTTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGa                                             <  2:1044487/101‑1 (MQ=255)
                                                    gATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCGTTCATCTTGACGa                                             >  1:3830165/1‑101 (MQ=255)
                                                    gATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGa                                             >  1:3036740/1‑101 (MQ=255)
                                                      tCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaa                                           <  1:1513540/101‑1 (MQ=255)
                                                      tCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaa                                           >  1:796449/1‑101 (MQ=255)
                                                        tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaa                                         <  2:651701/101‑2 (MQ=255)
                                                        tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaa                                         <  2:2298915/101‑2 (MQ=255)
                                                         gCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaag                                        >  2:1881565/1‑99 (MQ=255)
                                                         gCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaag                                        >  1:2741222/1‑99 (MQ=255)
                                                          cGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..agg                                     >  1:1577851/1‑101 (MQ=255)
                                                           gTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGACTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggg                                    >  2:317461/1‑100 (MQ=255)
                                                              tCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggtt                                 <  2:3240982/101‑5 (MQ=255)
                                                               ccACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggttg                                <  1:2443391/101‑6 (MQ=255)
                                                                   aTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgt                            <  2:890432/101‑3 (MQ=255)
                                                                    tCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtc                           >  1:906535/1‑98 (MQ=255)
                                                                     cGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcg                          <  1:422095/101‑5 (MQ=255)
                                                                        cATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtttcggtc                       >  1:254707/1‑94 (MQ=255)
                                                                         aTCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggtct                      <  1:214764/101‑9 (MQ=255)
                                                                            gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.gg                    >  1:3917286/1‑100 (MQ=255)
                                                                            gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.gg                    >  1:3008210/1‑100 (MQ=255)
                                                                             aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggt                   >  1:3070696/1‑99 (MQ=255)
                                                                             aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggt                   >  2:4344673/1‑99 (MQ=255)
                                                                             aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggt                   <  2:3062887/101‑3 (MQ=255)
                                                                              tCTTGCCCGCATAGGTCGAGAGCACCAGGCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtg                  >  1:733218/1‑98 (MQ=255)
                                                                              tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtg                  <  1:1380194/101‑4 (MQ=255)
                                                                              tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtg                  <  2:2105296/101‑4 (MQ=255)
                                                                               cTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtga                 >  1:1051045/1‑97 (MQ=255)
                                                                                ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgat                <  2:4159256/101‑6 (MQ=255)
                                                                                  gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatca              >  2:4334528/1‑94 (MQ=255)
                                                                                   cccGCATAGGGCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatcag             <  2:567713/101‑9 (MQ=255)
                                                                                    ccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatcaga            <  1:3185832/101‑10 (MQ=255)
                                                                                     cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatcagac           <  2:796367/101‑11 (MQ=255)
                                                                                     cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatcagac           <  2:1534291/101‑11 (MQ=255)
                                                                                       cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAtcagacag          >  2:3982373/1‑101 (MQ=255)
                                                                                       cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAtcagacag          >  1:2868463/1‑101 (MQ=255)
                                                                                        aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAgg         >  1:558420/1‑101 (MQ=255)
                                                                                        aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAgg         <  1:993373/101‑1 (MQ=255)
                                                                                        aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAgg         >  1:106419/1‑101 (MQ=255)
                                                                                        aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAgg         <  2:1220898/101‑1 (MQ=255)
                                                                                             tCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCg    >  2:2959551/1‑101 (MQ=255)
                                                                                             tCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCg    >  1:1447236/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGT  >  NC_014034/2177115‑2177305

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG