Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,186,002	G→C	100%	intergenic (+275/‑230)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,186,002	0	G→C	100.0%	64.7	20	intergenic (+275/‑230)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (12/8):  ref base (0/0):  total (12/8)

GCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTC.CGGGGGCGGA..G..CC.C.GCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAC  >  NC_014034/2185922‑2186098                                                                                         |                                                                                                 gCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTC.CGGGGGCGGA..GCCCC.C.GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAg                                                                               <  1:778662/101‑1 (MQ=2)            cccTTTCGCCTT.GCTCGAAGACCGCGGGGGGCGCGGGGGGGGA..G..CCGCGGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCaccgacc                                                                    <  1:3283140/101‑1 (MQ=2)                               ctgcccggggggTC.GGGGGGGGGA..GCCCC.C.GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTcg                                                 <  2:1075953/94‑1 (MQ=9)                                 acccggggggTC.CGGGGGCGGACCC..CC.C.GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTcgcg                                               <  2:697749/96‑1 (MQ=12)                                                             cc.c.GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGc                      <  2:822931/101‑1 (MQ=25)                                                                             cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc          >  2:4403638/10‑101 (MQ=255)                                                                             cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc          <  2:3410570/92‑1 (MQ=255)                                                                              ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCCGGACGGCTATGCCTTGAccc         >  1:2000193/9‑101 (MQ=255)                                                                              ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc         >  1:881241/9‑101 (MQ=255)                                                                               tcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCt        >  1:1567619/8‑101 (MQ=255)                                                                                cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt       <  2:2919072/95‑1 (MQ=255)                                                                                cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt       >  2:1291481/7‑101 (MQ=255)                                                                                 gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc      >  1:658283/6‑101 (MQ=255)                                                                                 gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc      >  1:555830/6‑101 (MQ=255)                                                                                  ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg     <  2:351680/97‑1 (MQ=255)                                                                                   gcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCgg    >  1:1096798/4‑101 (MQ=255)                                                                                    caaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGa   >  2:2280821/3‑101 (MQ=255)                                                                                     aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc  >  2:4099247/2‑101 (MQ=255)                                                                                     aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc  >  2:561689/2‑101 (MQ=255)                                                                                     aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc  >  1:3652626/2‑101 (MQ=255)                                                                                         |                                                                                                 GCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTC.CGGGGGCGGA..G..CC.C.GCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAC  >  NC_014034/2185922‑2186098 Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG