Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,186,012 G→A 100% intergenic (+285/‑220) mtlK → / → RCAP_rcc02028 mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,186,0120G→A100.0% 120.3 33intergenic (+285/‑220)mtlK/RCAP_rcc02028mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (24/9):  ref base (0/0):  total (24/9)

GCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTC.CGGGGGCGGA..G..CC.C.GCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGG  >  NC_014034/2185922‑2186107
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gCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTC.CGGGGGCGGA..GCCCC.C.GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAg                                                                                        <  1:778662/101‑1 (MQ=2)
           cccTTTCGCCTT.GCTCGAAGACCGCGGGGGGCGCGGGGGGGGA..G..CCGCGGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCaccgacc                                                                             <  1:3283140/101‑1 (MQ=2)
                              ctgcccggggggTC.GGGGGGGGGA..GCCCC.C.GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTcg                                                          <  2:1075953/94‑1 (MQ=9)
                                acccggggggTC.CGGGGGCGGACCC..CC.C.GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTcgcg                                                        <  2:697749/96‑1 (MQ=12)
                                                            cc.c.GGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGc                               <  2:822931/101‑1 (MQ=25)
                                                                            cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc                   >  2:4403638/10‑101 (MQ=255)
                                                                            cctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAcc                   <  2:3410570/92‑1 (MQ=255)
                                                                             ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCCGGACGGCTATGCCTTGAccc                  >  1:2000193/9‑101 (MQ=255)
                                                                             ctcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGAccc                  >  1:881241/9‑101 (MQ=255)
                                                                              tcgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCt                 >  1:1567619/8‑101 (MQ=255)
                                                                               cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt                <  2:2919072/95‑1 (MQ=255)
                                                                               cgggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCtt                >  2:1291481/7‑101 (MQ=255)
                                                                                gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc               >  1:658283/6‑101 (MQ=255)
                                                                                gggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTc               >  1:555830/6‑101 (MQ=255)
                                                                                 ggcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCg              <  2:351680/97‑1 (MQ=255)
                                                                                  gcaaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCgg             >  1:1096798/4‑101 (MQ=255)
                                                                                   caaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGa            >  2:2280821/3‑101 (MQ=255)
                                                                                    aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc           >  2:561689/2‑101 (MQ=255)
                                                                                    aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc           >  2:4099247/2‑101 (MQ=255)
                                                                                    aaaGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGAc           >  1:3652626/2‑101 (MQ=255)
                                                                                      agcccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCg         >  2:2348260/4‑101 (MQ=255)
                                                                                      agcccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCg         >  1:3563532/4‑101 (MQ=255)
                                                                                       gcccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCgg        >  2:2420369/3‑101 (MQ=255)
                                                                                       gcccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCgg        >  2:628297/3‑101 (MQ=255)
                                                                                         ccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGt      >  2:3406454/1‑101 (MQ=255)
                                                                                         ccGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGt      >  1:1449207/1‑101 (MQ=255)
                                                                                           gAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTg    >  1:3770449/1‑101 (MQ=255)
                                                                                            aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg   >  2:2050519/1‑101 (MQ=255)
                                                                                            aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg   >  2:3252077/1‑101 (MQ=255)
                                                                                            aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg   >  2:1462515/1‑101 (MQ=255)
                                                                                            aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg   >  1:609314/1‑101 (MQ=255)
                                                                                            aGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTgg   >  1:1037091/1‑101 (MQ=255)
                                                                                             ggCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTggg  <  1:4393999/101‑1 (MQ=255)
                                                                                                  |                                                                                               
GCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTC.CGGGGGCGGA..G..CC.C.GCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCAGGACGGCTATGCCTTGACCCTTCGGACCGGGTTGGG  >  NC_014034/2185922‑2186107

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG