Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 221,942 G→C 4.1% H239D (CAT→GAT)  RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034221,9420G→C 4.1% 8.1 ‑0.7 ‑0.1191H239D (CAT→GAT) RCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (86/97):  total (91/100)
Fisher's exact test strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.81e-01

Only 100 of 216 total aligned reads displayed.

ACGGTCGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATC  >  NC_014034/221842‑222041
                                                                                                    |                                                                                                   
    tCGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTg                                                                                                 <  1:2426308/101‑1 (MQ=255)
    tCGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTg                                                                                                 <  1:4331900/101‑1 (MQ=255)
    gcGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTg                                                                                                 <  2:3951195/100‑1 (MQ=255)
       aGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGc                                                                                              <  2:1411292/101‑1 (MQ=255)
       aGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGc                                                                                              <  2:4380411/101‑1 (MQ=255)
         ccGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCtc                                                                                            <  2:3022950/101‑1 (MQ=255)
          cGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCtcg                                                                                           <  1:508946/101‑1 (MQ=255)
           ggTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCtcgg                                                                                          >  2:4137652/1‑101 (MQ=255)
           ggTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCtcgg                                                                                          >  2:3382063/1‑101 (MQ=255)
            gTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCtcggt                                                                                         <  2:1021567/101‑1 (MQ=255)
               tCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCtcggtcgg                                                                                      <  1:1313998/101‑1 (MQ=255)
               tCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCAACGACGGTGGGCGTCGGATGCGTGGGCtcggtcgg                                                                                      <  1:4098587/101‑1 (MQ=255)
                cGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCgga                                                                                     <  2:422840/101‑1 (MQ=255)
                cGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCgga                                                                                     <  1:3141272/101‑1 (MQ=255)
                cGCCGGTGCCCGACACCGTCGCCGAGGCTGCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCgga                                                                                     <  1:3169516/101‑1 (MQ=255)
                 gCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggat                                                                                    <  2:3826642/101‑1 (MQ=255)
                 gCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggat                                                                                    <  2:3095386/101‑1 (MQ=255)
                 gCCGGTGCCCGACACCGTCGCCGAGGCTTCGGCGTTGGGGGTCAGCGTCGCCGGATCCCGCTCATCGACGGTGGGCGGCGGATGCGTGGGCtcggtcggtc                                                                                    >  1:3734544/1‑99 (MQ=255)
                  ccGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatc                                                                                   <  2:2418473/101‑1 (MQ=255)
                  ccGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatc                                                                                   >  2:3399520/1‑101 (MQ=255)
                    ggTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcgg                                                                                 <  1:3406758/101‑1 (MQ=255)
                         ccGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcggg                                                                            >  1:2207768/1‑101 (MQ=255)
                         ccGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcggg                                                                            <  1:2030949/101‑1 (MQ=255)
                            acacCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtcc                                                                         >  2:1717864/1‑101 (MQ=255)
                              acCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgg                                                                       >  2:3571311/1‑100 (MQ=255)
                                  tCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtggga                                                                   <  1:478697/101‑1 (MQ=255)
                                  gcGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtggga                                                                   <  2:885941/100‑1 (MQ=255)
                                     ccGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcg                                                                <  2:2391339/101‑1 (MQ=255)
                                      cGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcggggccgtgggatcgg                                                               >  2:860885/1‑101 (MQ=255)
                                       gAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggt                                                              >  2:1160995/1‑101 (MQ=255)
                                         ggCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcg                                                            <  2:1576670/101‑1 (MQ=255)
                                          gCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgg                                                           >  1:3469868/1‑101 (MQ=255)
                                           cTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcggg                                                          >  1:2794425/1‑101 (MQ=255)
                                           cTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcggg                                                          >  2:4039131/1‑101 (MQ=255)
                                            ttCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtcggtgggatcggtcgggt                                                         >  1:756821/1‑101 (MQ=255)
                                              cGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgggtcc                                                       >  1:2717931/1‑101 (MQ=255)
                                               ggTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgggtccg                                                      >  1:4228742/1‑101 (MQ=255)
                                                gtgtTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgggtccgt                                                     >  2:3614997/1‑101 (MQ=255)
                                                 tgtTGGTGGTCAGCGTCGCCTGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGGCggatcggtcgggtccgtgggatcggtcggatccgtg                                                    <  2:146380/101‑1 (MQ=255)
                                                 tgtTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgggtccgtg                                                    <  1:3684990/101‑1 (MQ=255)
                                                 tgtTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgggtccgtg                                                    >  1:2342066/1‑101 (MQ=255)
                                                 tgtTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCggatcggtcgggtccgtgggatcggtcgggtccgtg                                                    >  2:3148172/1‑101 (MQ=255)
                                                  gtTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGgatcggtcgggtccgtgggatcggtcgggtccgtgg                                                   <  1:4236293/101‑1 (MQ=255)
                                                  gtTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGgatcggtcgggtccgtgggatcggtcgggtccgtgg                                                   >  1:2299375/1‑101 (MQ=255)
                                                   ttGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGatcggtcgggtccgtgggatcggtcgggtccgtggg                                                  <  2:1816860/101‑1 (MQ=255)
                                                    tggtggTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGAtcggtcgggtccgtgggatcggtcgggtccgtggga                                                 <  1:534991/101‑1 (MQ=255)
                                                     ggtggtCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATcggtcgggtccgtgggatcggtcgggtccgtgggat                                                >  1:1326736/1‑101 (MQ=255)
                                                     ggtggtCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATcggtcgggtccgtgggatccgtcgggtccgtgggat                                                >  1:2936575/1‑101 (MQ=255)
                                                      ggggtCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCggtcgggtccgtgggatcggtcgggtccgtgggatc                                               <  2:3416810/99‑1 (MQ=255)
                                                       tggtCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGgtcgggtccgtgggatcggtcgggtccgtgggatcg                                              >  1:2649856/1‑101 (MQ=255)
                                                       tggtCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGgtcgggtccgtgggatcggtcgggtccgtgggatcg                                              <  2:2328442/101‑1 (MQ=255)
                                                          tCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCgggtccgtgggatcggtcgggtccgtgggatcggtc                                           >  2:1115933/1‑101 (MQ=255)
                                                          tCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCgggtccgtgggatcggtcgggtccgtgggatcggtc                                           <  2:1043157/101‑1 (MQ=255)
                                                           cAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGggtccgtgggatcggtcgggtccgtgggatcggtcg                                          <  1:4065570/101‑1 (MQ=255)
                                                            aGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGccgggtccgtgggctcggtcgggtccgcgggatcggcggg                                         >  2:2652984/1‑97 (MQ=255)
                                                            aGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGgtccgtgggatcggtcgggtccgtgggatcggtcgg                                         >  2:3802314/1‑101 (MQ=255)
                                                            aGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGgtccgtgggatcggtcgggtccgtgggatcggtcgg                                         <  2:1567353/101‑1 (MQ=255)
                                                            aGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGgtccgtgggatcgggcggggccgtgggatcggtcgg                                         >  2:800779/1‑101 (MQ=255)
                                                             gCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGa                                        >  1:1946164/1‑101 (MQ=255)
                                                              cGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGAt                                       >  2:3217367/1‑101 (MQ=255)
                                                                tCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTTGGATCGGTCGGATcc                                     <  1:3754101/101‑1 (MQ=255)
                                                                 cGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCg                                    >  1:1788671/1‑101 (MQ=255)
                                                                   ccGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTg                                  >  2:3874188/1‑101 (MQ=255)
                                                                   ccGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTg                                  <  1:662528/101‑1 (MQ=255)
                                                                      ggtCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGa                               <  1:1053284/99‑1 (MQ=255)
                                                                        tCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtc                             <  1:1649484/101‑1 (MQ=255)
                                                                          gcgcTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcgg                           <  2:2550190/101‑1 (MQ=255)
                                                                           ggcTCATGGGCGGTGGGCGGCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggt                          <  1:1279094/100‑1 (MQ=255)
                                                                            gcTCATCGGCGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtc                         <  1:3515409/101‑1 (MQ=255)
                                                                            gcTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtc                         >  2:2771580/1‑101 (MQ=255)
                                                                            gcTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtc                         >  2:1788020/1‑101 (MQ=255)
                                                                             cTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGGTCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtcg                        >  1:2087564/1‑101 (MQ=255)
                                                                                aTCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGt                     <  1:1586585/101‑1 (MQ=255)
                                                                                  cGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTcc                   <  1:825498/101‑1 (MQ=255)
                                                                                  cGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTcc                   <  1:2824776/101‑1 (MQ=255)
                                                                                   gACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCg                  <  2:4059339/101‑1 (MQ=255)
                                                                                    aCGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGt                 <  1:3874441/101‑1 (MQ=255)
                                                                                    aCGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGt                 <  2:2936354/101‑1 (MQ=255)
                                                                                     tgggctctgTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGtc                <  2:3171346/93‑2 (MQ=11)
                                                                                     cGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTGGGATCCGTGGGGtcgggcggcgcccgg                >  2:784879/1‑94 (MQ=21)
                                                                                      ggTGGGCGGCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgg               <  1:3243246/101‑1 (MQ=255)
                                                                                       gTGGGCGGCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTggg              <  2:2322869/101‑1 (MQ=255)
                                                                                        gatcggTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGCGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTcggg             >  2:1273889/6‑100 (MQ=11)
                                                                                         gggCGTCGGAGGGGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAt            <  2:1830900/101‑1 (MQ=255)
                                                                                          tcggTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGtc           <  1:4164851/98‑1 (MQ=1)
                                                                                          tcggTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGtc           <  1:2468774/98‑1 (MQ=1)
                                                                                          tccGTCGGGTCCGGGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGtc           <  1:2212989/99‑1 (MQ=11)
                                                                                          ggCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtc           <  2:1344618/101‑1 (MQ=255)
                                                                                          ggCGTCGGATGCGTGGGCTCGGTCGGATCGGGCGGGTCCGTGGGATAGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtg           >  1:3971212/1‑100 (MQ=255)
                                                                                           tggTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGtcc          <  1:4118884/99‑2 (MQ=11)
                                                                                           gCGTGGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGGCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcg          <  2:3272893/101‑1 (MQ=255)
                                                                                           gCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcg          <  1:3382300/101‑1 (MQ=255)
                                                                                            cGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTAGGGGCCGTGGGAtcgg         >  2:3301221/1‑101 (MQ=255)
                                                                                             gTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGtccgt        >  2:203224/1‑101 (MQ=11)
                                                                                             gTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggt        <  2:2047280/101‑1 (MQ=255)
                                                                                                ggATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcgg     >  2:2812592/1‑101 (MQ=255)
                                                                                                ggATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcgg     >  1:2159432/1‑101 (MQ=255)
                                                                                                ggATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGCGCGAACGCACGGATCCGAGGGATCGGTCGGGTCCGTGGGAtcggtcgg     >  2:3681979/1‑101 (MQ=18)
                                                                                                 gATCCGTGGGATCGGTGGGTTCCGTCGGATCCGTGGGATCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGAtcggtcggg    <  2:4422411/101‑2 (MQ=11)
                                                                                                   tGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTc  >  2:4180794/1‑101 (MQ=255)
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ACGGTCGAGCCGGTTTCGCCGGTGCCCGACACCGTCGCCGAGGCTTCGGTGTTGGTGGTCAGCGTCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATC  >  NC_014034/221842‑222041

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG