Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,078 C→G 3.3% T193T (ACG→ACC RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0780C→G 3.3% 15.5 ‑0.3 ‑0.0381T193T (ACG→ACCRCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/7):  ref base (197/171):  total (203/178)
Fisher's exact test strand distribution p-value = 7.79e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.06e-01

Only 100 of 620 total aligned reads displayed.

GATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGC  >  NC_014034/221975‑222178
                                                                                                       |                                                                                                    
   cggtcgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtcc                                                                                                      <  1:1684638/101‑1 (MQ=11)
    ggtcgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCGGTGGGATCGGGCGGATGCGTGGGGTCGGTCGGGTGGGAAGGGTCGtttgggtccg                                                                                                     >  2:1912327/1‑101 (MQ=14)
    gggcgggtccgtcggatcggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGgtcgggtccg                                                                                                     >  1:2558968/4‑101 (MQ=11)
     gtcgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGGTCGggcgggcccgc                                                                                                    >  1:2697121/1‑100 (MQ=11)
     gtcggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgt                                                                                                    <  1:1108714/101‑1 (MQ=255)
      tcgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtc                                                                                                   <  1:1284586/101‑1 (MQ=11)
       cgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtcg                                                                                                  <  2:3862901/101‑1 (MQ=11)
        ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgg                                                                                                 <  1:4433937/98‑1 (MQ=255)
        ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgg                                                                                                 >  1:3284457/4‑101 (MQ=255)
        ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgg                                                                                                 <  2:4195928/98‑1 (MQ=255)
         gatccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGTCGgtcggggccgggggg                                                                                                >  2:868217/3‑96 (MQ=14)
         gatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgga                                                                                                <  1:2888122/99‑2 (MQ=255)
         gatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgga                                                                                                >  2:3301038/3‑100 (MQ=255)
          atccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggat                                                                                               <  1:1001929/100‑3 (MQ=255)
           tccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggatc                                                                                              >  2:2564293/1‑101 (MQ=255)
            ccgtggggtccgtcggAGCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtggggtcc                                                                                             <  2:1312021/101‑1 (MQ=11)
              gtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcggggccgtcggatcggg                                                                                           >  1:1518380/1‑98 (MQ=14)
              gtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTcgggtccgtcggatccgt                                                                                           >  2:1768918/1‑101 (MQ=11)
               tggggtccgtcggATCCGGGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgtc                                                                                          <  1:3930447/101‑2 (MQ=11)
               tgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggtc                                                                                          <  2:2007889/101‑2 (MQ=11)
               tgcgctcgttcggACCCGTGGGTGTTGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTcggatccgtgggatcggtc                                                                                          <  2:4053246/96‑2 (MQ=2)
                ggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgtcg                                                                                         >  2:706921/1‑99 (MQ=11)
                 gggtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTggg                                                                                        >  2:1417383/10‑101 (MQ=11)
                 ggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTggg                                                                                        <  2:2310957/101‑1 (MQ=14)
                 ggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTcgg                                                                                        >  1:3044360/1‑101 (MQ=11)
                  gatgggggggATCCGGGGGATGGGTCGGGTCCGTGGGATCGGGGGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGa                                                                                       <  1:2153472/93‑1 (MQ=11)
                   gtccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGGTCGGTGGGac                                                                                      >  1:641517/5‑100 (MQ=14)
                   atcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAt                                                                                      <  2:104324/101‑1 (MQ=14)
                    tcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtc                                                                                     <  1:1181490/101‑1 (MQ=11)
                    tcggtcggATCCGTGGGATCGGTCGGATCCGTGGGACCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtc                                                                                     >  1:1227944/1‑101 (MQ=11)
                    tccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGGCGGGTCCGTGGGAtc                                                                                     >  2:2202059/7‑101 (MQ=11)
                    tccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGagc                                                                                     >  1:1758665/4‑99 (MQ=14)
                     cggtcggATCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGgttcggtccggggggtctgccgggtag                                                                                    >  2:1192133/1‑91 (MQ=11)
                     cggtcggATCCATGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtcc                                                                                    >  1:1385126/1‑100 (MQ=11)
                     ccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAtcg                                                                                    >  1:924420/6‑101 (MQ=14)
                     ccgtcggTTCCGTGGGTTCGGTCGGATGCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtcc                                                                                    <  2:3395885/99‑2 (MQ=11)
                       gtcgggggcGTGGGATGGGTCGGGGCCGTGGGGTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggt                                                                                  <  1:3876448/93‑1 (MQ=11)
                       gtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGGCGGTGGGGtgggt                                                                                  >  2:398447/1‑101 (MQ=11)
                        tcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtc                                                                                 <  2:967360/101‑1 (MQ=14)
                        tcggAGCCGTGGGGGCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtccgtg                                                                                 <  2:611057/101‑2 (MQ=11)
                        gcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtc                                                                                 <  1:3154567/100‑1 (MQ=14)
                         cggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtcg                                                                                >  2:3206606/1‑101 (MQ=14)
                         cggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtccgtgg                                                                                >  1:1344907/1‑99 (MQ=11)
                          ggATCCGTGGGGTCCGTCGGGTCCGTGGGATAGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtccgtggg                                                                               >  1:4003287/1‑101 (MQ=11)
                          ggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTGGGAtccgtggg                                                                               >  2:3077946/1‑101 (MQ=14)
                           ggtCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGCCGGGTCCGTGGGGAGCGTCGGAtcagtagaa                                                                              >  1:2743936/3‑97 (MQ=11)
                            ttCCGTGGGACGGGTCGGGTCCGTGGGATCGGTCGGCTCCGTGGGATCGTTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAt                                                                             <  2:3928617/100‑1 (MQ=1)
                            gtgcggtggATCGGTGGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggtcgggt                                                                             <  1:370557/94‑3 (MQ=14)
                            aTCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGAt                                                                             <  1:491034/101‑1 (MQ=11)
                              ccGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTcc                                                                           >  1:352794/1‑101 (MQ=14)
                               cGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTGGGATCGGTCGGGTCCg                                                                          >  1:2337750/1‑101 (MQ=255)
                                gtaggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGTGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGt                                                                         <  2:3509258/98‑1 (MQ=14)
                                 tGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTg                                                                        >  2:2999553/1‑101 (MQ=14)
                                  gggTTCGGTCGGTTCCGTGGGTCCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGACCGATcccacg                                                                       <  2:3759876/101‑5 (MQ=11)
                                  gggATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGtcg                                                                       >  2:2875497/1‑99 (MQ=11)
                                   ggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTggg                                                                      >  1:4082981/1‑101 (MQ=255)
                                    gttaagtcgtGTCCCTGNGTTCGGGNGGGTNCGTGGAATCGGTCGGATCGGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCNGTCGGGTCCGTAGGa                                                                     <  1:850519/96‑1 (MQ=0)
                                    gATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTGGGGTCCggcgga                                                                     >  2:2512403/1‑96 (MQ=14)
                                     gtccggggggGCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAt                                                                    <  1:989948/94‑1 (MQ=18)
                                     aTCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAt                                                                    >  1:3964262/1‑101 (MQ=255)
                                      tccgtggggTCCGTTGGTTCGGTGGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtc                                                                   <  2:2768983/95‑1 (MQ=18)
                                       tggtgggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcg                                                                  <  2:4116347/100‑1 (MQ=255)
                                        ggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcgg                                                                 >  2:314854/1‑101 (MQ=14)
                                        ggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcgg                                                                 >  2:1491012/1‑101 (MQ=14)
                                         gtcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGACGGGTCCGTGGGAtcggt                                                                >  1:2412634/1‑101 (MQ=17)
                                          tcggGTCCGTGGGATCGGTCGGATCGGTGGGGTCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc                                                               <  1:3464598/101‑1 (MQ=17)
                                           ggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtcg                                                              >  1:1440037/2‑101 (MQ=255)
                                           cggTTCCGTGGGATGGGTCGGATCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcg                                                              <  1:3393195/101‑1 (MQ=14)
                                           cggGTCCGTGGGATCGGGCGGATCCGTGGGATCGGTCGGGGCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcg                                                              <  2:24085/101‑1 (MQ=17)
                                           cggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcgggcg                                                              >  2:2623071/1‑101 (MQ=14)
                                            ggggcggTGGGGTGGGTCGGATGCGTGGGAGCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcgg                                                             <  1:596042/95‑1 (MQ=14)
                                              gTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGt                                                           >  2:2138098/1‑101 (MQ=17)
                                               tCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCggggc                                                          >  2:491365/1‑99 (MQ=17)
                                               tCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTc                                                          <  1:2999774/101‑1 (MQ=17)
                                                ccGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTcc                                                         <  1:2624539/101‑1 (MQ=14)
                                                   tcggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGCCCGACGGGGCCGCGGGGTCGGGCGGGGGCGGCGGATTGGTGGGGTCCGTg                                                      >  2:1677357/3‑101 (MQ=9)
                                                    gggATCGGTCGGATCCGTGAAATCGGTCGGGCCCGTGGGCTCGGTCGGATCCGTGGGGCCCGTGGGACGGGCCGGGTCCGACCGCTCGGTCggggcggtat                                                     >  2:437310/1‑94 (MQ=2)
                                                     ggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTggg                                                    >  1:694404/1‑101 (MQ=18)
                                                       aTCGGTCGGATCGGTGGGATCGGTCGGGGCCGTGGGACCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggat                                                  <  1:1975970/101‑3 (MQ=14)
                                                        gccgttggATCGGTGGGAGCGGTCGGGGCCGTGGGATCGGTCGGATCGGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatc                                                 <  1:2019117/95‑4 (MQ=11)
                                                        acggtcggAGCCGTGGGACCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatc                                                 <  1:2251199/100‑4 (MQ=14)
                                                         cggtgggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGGCGGATCCGTGGGGTCCGTGGGATCGGTGGGGTCCGTGGGGTCGGCCGGGTCCgcaggaacc                                                >  2:1351040/1‑93 (MQ=14)
                                                         cggtcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtg                                                >  1:4351640/1‑101 (MQ=255)
                                                            tcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtc                                             <  1:758390/101‑8 (MQ=11)
                                                            tcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtc                                             <  1:2821956/101‑8 (MQ=11)
                                                             gcggtCCGTGGGGTCGGTCGGGTCCGTAGGATCCGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtggtgg                                            <  2:3589644/97‑1 (MQ=255)
                                                              ggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtcgg                                           <  2:1548767/101‑10 (MQ=11)
                                                                  ccGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcg                                       >  1:4136012/1‑101 (MQ=255)
                                                                  ccGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGGCCGTGGGGCTGGTGGGTtcg                                       >  1:3773651/1‑101 (MQ=255)
                                                                   cGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCCTGGGGCTGGTGGGAtcgg                                      >  2:2938235/1‑101 (MQ=255)
                                                                    gTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcggt                                     >  1:1810817/1‑101 (MQ=255)
                                                                    gTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGGGGGAtcggg                                     >  1:2483017/1‑100 (MQ=255)
                                                                         aTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGGTGGGTCCGTGGGCTCGGTGGGGTCAGGGGGACCGCTGGGaaccggtgata                                >  2:2851210/1‑91 (MQ=0)
                                                                                gggTCCGTGGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTc                         <  1:1591584/101‑1 (MQ=255)
                                                                                gggTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTc                         >  1:3588800/1‑101 (MQ=255)
                                                                                  gTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgc                       >  2:1592523/1‑101 (MQ=255)
                                                                                    ccGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGGCGGACCGCTGTcgccg                     >  1:3194395/1‑101 (MQ=255)
                                                                                          gATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGccgccgccg               >  2:172008/1‑101 (MQ=255)
                                                                                                  gggtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGAcc       >  2:2027120/1‑101 (MQ=255)
                                                                                                    gtccgtcgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCa     <  1:4252548/101‑1 (MQ=255)
                                                                                                       |                                                                                                    
GATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGC  >  NC_014034/221975‑222178

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG