Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,081 C→G 26.6% P192P (CCG→CCC RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0810C→G26.6% 252.7 ‑3.0 ‑0.0374P192P (CCG→CCCRCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (43/57):  ref base (150/124):  total (193/181)
Fisher's exact test strand distribution p-value = 4.76e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.92e-01

Only 100 of 582 total aligned reads displayed.

GATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGG  >  NC_014034/221975‑222181
                                                                                                          |                                                                                                    
      tcggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtc                                                                                                      <  1:2536023/101‑1 (MQ=11)
       cgtatcggtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCTGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcg                                                                                                     <  2:2299230/94‑1 (MQ=14)
       cggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcg                                                                                                     >  1:3655479/1‑101 (MQ=255)
        gggtccgtggggtccgtcggGTCCGGGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGgtgggatccgtggg                                                                                                    <  2:3879607/101‑1 (MQ=1)
        gggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTGGGATCGGTGGGGTGGGTGGGGGCGGTGGGATCGggcgtgtccgtggg                                                                                                    >  2:102450/1‑101 (MQ=11)
        ggatccgtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGAGCGgtcgggtccgtcgg                                                                                                    >  1:1288988/4‑101 (MQ=11)
          atccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggat                                                                                                  <  1:1001929/100‑3 (MQ=255)
           tccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCTTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtggggtc                                                                                                 <  2:2940056/101‑1 (MQ=11)
            tcgtaggatcgttcggTGCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCgtggggtccgtcgggtcc                                                                                                <  2:560957/100‑1 (MQ=14)
            ccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggatcg                                                                                                <  2:2852939/101‑2 (MQ=255)
               tggggtccgtcggATCCGGGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgtc                                                                                             <  1:3930447/101‑2 (MQ=11)
               tggggtccgtaggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTACGTGGGATAGgtcgggtccgtggggtaagtc                                                                                             >  1:2275210/1‑96 (MQ=11)
               tgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggtc                                                                                             <  2:2007889/101‑2 (MQ=11)
               tgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGtcgggtccgtcgggtccggg                                                                                             >  2:1258929/1‑99 (MQ=11)
               taggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTg                                                                                             >  1:970207/3‑101 (MQ=14)
               tacgatccgtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTg                                                                                             <  2:1863423/98‑1 (MQ=14)
                  ggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGa                                                                                          >  1:1395162/6‑101 (MQ=11)
                  gatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTGGGATCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGa                                                                                          >  2:2691179/1‑101 (MQ=11)
                    tcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtc                                                                                        <  1:1181490/101‑1 (MQ=11)
                    tcggtcggATCCGTGGGATCGGTCGGATCCGTGGGACCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtc                                                                                        >  1:1227944/1‑101 (MQ=11)
                     cggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcg                                                                                       >  1:704906/1‑101 (MQ=11)
                     cggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGGTCCNTGGGGTCCGGgggcgcg                                                                                       >  2:1137410/1‑97 (MQ=11)
                     ccgtcggATGCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtcc                                                                                       <  2:2890154/99‑2 (MQ=11)
                      ggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcgg                                                                                      <  1:1782474/101‑1 (MQ=11)
                      ggtcggGGCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcgg                                                                                      <  1:272395/101‑1 (MQ=11)
                      cgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGGtcgg                                                                                      >  1:3053644/5‑101 (MQ=14)
                        tcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtc                                                                                    <  2:967360/101‑1 (MQ=14)
                        gcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtc                                                                                    <  1:3154567/100‑1 (MQ=14)
                         cggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtcg                                                                                   >  2:3206606/1‑101 (MQ=14)
                         cggACCCGTGGGATCGGTTGGACCCGTGGGACCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtccgtgg                                                                                   <  2:1480387/101‑3 (MQ=11)
                          ggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTGGGAtccgtggg                                                                                  >  2:3077946/1‑101 (MQ=14)
                           ggtCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggtcggg                                                                                 >  1:1611775/3‑100 (MQ=11)
                           gctCCGCGGGGTCGGTGGGATCCGTGGGATCGGTCGGATCCGTGGGATCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggtcggg                                                                                 <  2:1742818/99‑2 (MQ=14)
                            aTCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGAt                                                                                <  1:491034/101‑1 (MQ=11)
                             tCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTAGGATGGGCCGGGTCGGTGGGGTCGGTGGGAtcgggagggtc                                                                               >  1:4424391/1‑94 (MQ=11)
                             tCCGGGGGATCGGTCGGGTCCGTGGGATCGGACGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTc                                                                               <  1:1461891/101‑1 (MQ=14)
                              ccGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTcc                                                                              <  2:2192648/101‑1 (MQ=14)
                                gtaggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGTGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGt                                                                            <  2:3509258/98‑1 (MQ=14)
                                gtaggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGt                                                                            <  1:1726622/98‑1 (MQ=14)
                                 tGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGCCCGTGGGATCGGTCGGGTCCGTg                                                                           >  1:2829870/1‑101 (MQ=14)
                                  gggTTCGGTCGGTTCCGTGGGTCCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGACCGATcccacg                                                                          <  2:3759876/101‑5 (MQ=11)
                                   ggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTcgg                                                                         <  1:1842783/101‑1 (MQ=14)
                                   ggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTggg                                                                         >  1:4082981/1‑101 (MQ=255)
                                   ggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTAGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGGggg                                                                         >  2:803743/1‑101 (MQ=18)
                                    gATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTGGGGTCCggcgga                                                                        >  2:2512403/1‑96 (MQ=14)
                                    gATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGa                                                                        <  2:4081057/101‑1 (MQ=255)
                                     gtcggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAt                                                                       <  1:1995287/100‑1 (MQ=14)
                                     aTCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCAGGTCCGTGGGAt                                                                       <  1:2429005/101‑1 (MQ=14)
                                     aTCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAt                                                                       >  1:3964262/1‑101 (MQ=255)
                                      tccgtggggTCCGTTGGTTCGGTGGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtc                                                                      <  2:2768983/95‑1 (MQ=18)
                                       cggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGACCCGTGGGGCCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGGtcg                                                                     >  1:3001006/1‑101 (MQ=14)
                                        ggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcgg                                                                    >  2:314854/1‑101 (MQ=14)
                                        cgtggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcgg                                                                    >  2:3304973/5‑101 (MQ=255)
                                          tcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcggtc                                                                  <  1:13538/101‑1 (MQ=17)
                                          gggggTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtc                                                                  <  1:234010/99‑1 (MQ=255)
                                           cggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCGGTGGGGTCCGTGGGATCGGACGGGTCCGGGGGAtcgggcg                                                                 >  2:2566602/1‑101 (MQ=14)
                                           cggGTCCGTGGGATCGGGCGGATCCGTGGGATCGGTCGGGGCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtcg                                                                 <  2:24085/101‑1 (MQ=17)
                                            gggTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGGtcggttgg                                                                >  2:1387931/1‑101 (MQ=14)
                                            gggTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCCGTGGGGTCCGTCGAGTCAGTGGGACCACTCGGGTCCGGCCGAtcgctcgg                                                                >  1:3831314/1‑101 (MQ=9)
                                              gTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGCTCCGTCGGTGCCGTGGGCGCCGTCGGGGCGGTGGGGtgggtggagg                                                              >  2:4153104/1‑96 (MQ=2)
                                              gTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGt                                                              >  1:4253719/1‑101 (MQ=255)
                                              gTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGt                                                              <  1:3390478/101‑1 (MQ=17)
                                                ccGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTGGGATCCGTGGGGTGCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGAcc                                                            >  2:4403865/1‑101 (MQ=14)
                                                 cGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGCCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCg                                                           >  1:4382876/1‑101 (MQ=255)
                                                 cGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCg                                                           <  1:2669204/101‑1 (MQ=11)
                                                 cGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCg                                                           <  1:661325/101‑1 (MQ=17)
                                                 cGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGGCCg                                                           >  2:490982/1‑101 (MQ=17)
                                                    gggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgg                                                        >  2:4002343/1‑101 (MQ=255)
                                                     ggATGGGTCGGGGTCGTGGGTTCGGTCGGATGCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTcgg                                                       <  1:1096717/101‑1 (MQ=11)
                                                      gATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggg                                                      <  1:4002599/101‑1 (MQ=255)
                                                      gATCGGTCGGATCGGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTggga                                                      <  2:3930788/101‑2 (MQ=17)
                                                       gtcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggat                                                     <  2:42591/100‑3 (MQ=14)
                                                       aTCGGCCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGCTCGGTCGGGCCCGCGGGATCGGTCGGGGCCGGAGGACCGGACGGGGCCGTgggcg                                                     >  2:1634616/1‑99 (MQ=2)
                                                          ggtcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtgg                                                  >  2:3007241/1‑101 (MQ=255)
                                                            tggggtCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtggtg                                                <  1:2566765/96‑1 (MQ=255)
                                                            tcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGAGGGGCtggtg                                                >  1:1527235/1‑101 (MQ=255)
                                                            tcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggtc                                                >  1:60605/1‑94 (MQ=14)
                                                            tcggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtc                                                <  1:758390/101‑8 (MQ=11)
                                                             gcggtCCGTGGGGTCGGTCGGGTCCGTAGGATCCGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtggtgg                                               <  2:3589644/97‑1 (MQ=255)
                                                             cggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggtcg                                               <  1:2907312/101‑9 (MQ=14)
                                                             cggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTGGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtcg                                               >  1:3756484/1‑93 (MQ=11)
                                                             cggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtcg                                               >  1:3453009/1‑93 (MQ=11)
                                                              gggtCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTggg                                              >  2:2026170/4‑101 (MQ=255)
                                                              ggATCCGTGTGATCGGTCGGGTCCGTTGGCTCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggtcgg                                              <  2:1717620/101‑10 (MQ=14)
                                                              ggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggtcgg                                              >  2:342741/1‑92 (MQ=14)
                                                              ggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGGTCCGTggggtcggtcgg                                              >  1:3283536/1‑93 (MQ=14)
                                                              ggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtcgg                                              <  2:1548767/101‑10 (MQ=11)
                                                                      gggATCGGTAGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGAGGGATCGGTCGGGACAGTGGGGCTGGTGGGAtcggtag                                      >  1:2168323/1‑99 (MQ=25)
                                                                        gATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATGGGTCGGGTCCGTGGGGCTGGTGGGGttggtgggc                                    >  1:801549/1‑100 (MQ=21)
                                                                        gATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGa                                    >  1:3572203/1‑101 (MQ=255)
                                                                         aTCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGAc                                   >  1:2038487/1‑101 (MQ=255)
                                                                             gtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCt                               <  1:3508513/101‑1 (MQ=255)
                                                                                    ccGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGGCGGACCGCTGTcgccg                        >  1:3194395/1‑101 (MQ=255)
                                                                                     cGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccgc                       >  1:883172/1‑101 (MQ=255)
                                                                                         ggATCGGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCgccgccgcc                   <  1:4153365/101‑1 (MQ=255)
                                                                                           aTCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCgg                 <  2:693589/101‑1 (MQ=255)
                                                                                              ggtccgGTCCTTCGGGTCGGTGGGATCGGACGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATTGGTCGGACCGCTGTGGCCGCCGCCGGACa              <  2:1327482/101‑1 (MQ=25)
                                                                                                         tcgggtccttGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAggccg   >  1:2766618/1‑101 (MQ=255)
                                                                                                         gcgggtccgtGGGATCGGGCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAggccg   <  2:1587807/100‑1 (MQ=255)
                                                                                                          cgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAggccgg  >  2:2109745/1‑101 (MQ=255)
                                                                                                          |                                                                                                    
GATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGG  >  NC_014034/221975‑222181

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG