Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,087 C→G 8.7% T190T (ACG→ACC)  RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0870C→G 8.7% 66.1 ‑3.7 ‑1.1367T190T (ACG→ACC) RCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/23):  ref base (168/167):  total (177/190)
Fisher's exact test strand distribution p-value = 2.50e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.69e-02

Only 100 of 518 total aligned reads displayed.

CGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGAC  >  NC_014034/221978‑222187
                                                                                                             |                                                                                                    
          cgtgggcgctgtcggATCCGTGGGATCTGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGtcgggtccgtggggtccg                                                                                                     <  2:3445409/101‑1 (MQ=11)
            tggggtccgtcggATCCGGGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgtc                                                                                                   <  1:3930447/101‑2 (MQ=11)
            tgggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTg                                                                                                   <  1:945782/101‑1 (MQ=14)
            tgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggtc                                                                                                   >  1:1471970/1‑100 (MQ=11)
            taggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTg                                                                                                   >  1:3554764/3‑101 (MQ=14)
             ggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTcgggtccgtggggtccgtcg                                                                                                  >  2:706921/1‑99 (MQ=11)
             gggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggtcg                                                                                                  >  1:2618002/1‑99 (MQ=11)
             gggatcggtcggATCCGTAGGTTCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTgg                                                                                                  <  2:1255515/101‑1 (MQ=11)
               ggtccgtcggATCCGGGGGGTCCGTCGGGTCCGGGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGa                                                                                                <  1:2300523/96‑1 (MQ=11)
               gatgggggggATCCGGGGGATGGGTCGGGTCCGTGGGATCGGGGGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGa                                                                                                <  1:2153472/93‑1 (MQ=11)
               gatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGGCCGTCCGGGCCGTgggg                                                                                                >  2:3106460/1‑100 (MQ=11)
               gatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTGGGATCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGa                                                                                                >  2:2691179/1‑101 (MQ=11)
                gtccgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGCCCGTCGGGTCCGTGGGAt                                                                                               >  1:736895/8‑101 (MQ=14)
                atcggtcggGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTAGGATCCGTGGGCTCGCcggggtccctggggtccgccggac                                                                                               >  2:1649837/1‑95 (MQ=2)
                 tcagtcggGTCCGTCGGATCGGTCGGGGCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtc                                                                                              <  1:1016379/98‑1 (MQ=11)
                  cggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGGTCCGTGGGGGCCGTGGGAccg                                                                                             >  2:3511269/1‑101 (MQ=11)
                  cggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGGCCGTGGGAtcg                                                                                             >  2:4164590/1‑101 (MQ=11)
                  cggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGAtcc                                                                                             <  1:674500/101‑2 (MQ=11)
                  ccgtcggATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtcc                                                                                             >  1:1216034/3‑100 (MQ=11)
                  ccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtcc                                                                                             <  1:589980/99‑2 (MQ=11)
                  cagtgcgATGCGTCGGCTCCGTAGGATGCGTGGGATCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAtcg                                                                                             <  2:2153613/95‑1 (MQ=17)
                   ggtcggGGCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcgg                                                                                            <  1:272395/101‑1 (MQ=11)
                   cgtgggATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGGtcgg                                                                                            >  1:3053644/5‑101 (MQ=14)
                     tcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtc                                                                                          >  2:3197700/1‑101 (MQ=14)
                     tcggAGCCGTGGGGGCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtccgtg                                                                                          <  2:611057/101‑2 (MQ=11)
                     gcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtc                                                                                          <  1:3154567/100‑1 (MQ=14)
                      cggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCATGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGAtcggtcg                                                                                         <  1:3350083/101‑1 (MQ=14)
                        ggtCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggtcggg                                                                                       >  1:1611775/3‑100 (MQ=11)
                         ttCCGTGGGACGGGTCGGGTCCGTGGGATCGGTCGGCTCCGTGGGATCGTTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAt                                                                                      <  2:3928617/100‑1 (MQ=1)
                         gtgcggtggATCGGTGGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGAtcggtcgggt                                                                                      <  1:370557/94‑3 (MQ=14)
                         gtCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGGtcggtcgggg                                                                                      >  1:1819042/2‑99 (MQ=11)
                         aTCCGTGGGATCTGTCAGTTCCGTGGGATCGGTCGGATTCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAt                                                                                      <  1:1759290/101‑1 (MQ=11)
                         aTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAt                                                                                      >  1:454812/1‑101 (MQ=11)
                          tctgTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATc                                                                                     <  2:1750139/98‑1 (MQ=11)
                           cggtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGAGCGGTCGGACcc                                                                                    >  2:289075/6‑101 (MQ=17)
                            ggtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCg                                                                                   >  1:2449161/5‑101 (MQ=17)
                            ggtcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCg                                                                                   >  2:2960407/5‑101 (MQ=17)
                            ggTGGGTTCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCg                                                                                   <  1:3253409/100‑1 (MQ=18)
                             gTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTGGGATCCGTGGGGTCCGTGGGAtcggtcggggcggg                                                                                  >  2:2018662/1‑95 (MQ=14)
                             gTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTGGGGTCCgg                                                                                  >  1:4231758/1‑100 (MQ=14)
                              tcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTg                                                                                 >  1:4034396/3‑101 (MQ=17)
                              taggATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGtc                                                                                 <  2:4260957/99‑2 (MQ=14)
                              tGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGCCCGTGGGATCGGTCGGGTCCGTg                                                                                 >  1:2829870/1‑101 (MQ=14)
                              gcggATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTg                                                                                 <  1:786881/99‑1 (MQ=255)
                               gggTTCGGTCGGTTCCGTGGGTCCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGACCGATcccacg                                                                                <  2:3759876/101‑5 (MQ=11)
                                 gATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGa                                                                              <  2:4081057/101‑1 (MQ=255)
                                  ggcggtgggGTCCGTGGGAGCGGGCGGGGCCGTGGGGTCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAt                                                                             <  1:2378715/99‑1 (MQ=18)
                                  aTCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAt                                                                             >  1:3964262/1‑101 (MQ=255)
                                   tcggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtc                                                                            >  2:1007148/1‑101 (MQ=14)
                                   tccgtggggTCCGTTGGTTCGGTGGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtc                                                                            <  2:2768983/95‑1 (MQ=18)
                                    gggtgggGTCCGGGGGATCGGTGGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcg                                                                           <  1:875828/100‑1 (MQ=21)
                                    cggtcggGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcg                                                                           >  1:3987665/1‑101 (MQ=17)
                                    cggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGACCCGTGGGGCCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGGtcg                                                                           >  1:3001006/1‑101 (MQ=14)
                                     ggtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcgg                                                                          >  2:314854/1‑101 (MQ=14)
                                      gtcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGGGGGAtcggt                                                                         >  2:1879785/1‑101 (MQ=14)
                                       tcggGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGAtcggtc                                                                        <  1:13538/101‑1 (MQ=17)
                                         gggTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGGtcggttgg                                                                      >  2:1387931/1‑101 (MQ=14)
                                          ggTCCTTGCGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCggg                                                                     <  2:2337600/101‑1 (MQ=255)
                                           gtgcgggggATCGGGCGGGCCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTGGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGt                                                                    <  1:4404137/95‑1 (MQ=18)
                                           gTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGt                                                                    >  1:4101506/1‑101 (MQ=255)
                                            tCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTc                                                                   >  1:130816/1‑101 (MQ=11)
                                             ccGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTGGGATCCGTGGGGTGCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGAcc                                                                  >  2:4403865/1‑101 (MQ=14)
                                              cgccggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCg                                                                 >  1:2555210/5‑101 (MQ=17)
                                              cGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCGCTGGGATCGGTCGGGGCCg                                                                 >  2:960259/1‑101 (MQ=18)
                                              cGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCg                                                                 >  2:1314438/1‑101 (MQ=255)
                                              cGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCg                                                                 <  1:2669204/101‑1 (MQ=11)
                                               gTGGTAACGGTCGGACCCGTGGGATCGGTCGGGCCCGTGGGATCCGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGt                                                                <  2:2569311/101‑1 (MQ=17)
                                                tGGGATCGGTCGGATCCGTCGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGta                                                               <  2:2077541/101‑2 (MQ=17)
                                                 ggggctggtcagATCCGGGGGATCTGTGGGGTCCGTGGGACCCGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGtag                                                              <  2:787694/95‑3 (MQ=9)
                                                 cggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGGCGGGTCGGTGGGGTCGGTCGGGTCCGTCGGATGGGTCGGGTCCGGgg                                                              >  2:1473248/2‑101 (MQ=14)
                                                  ggATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTggg                                                             <  1:3469310/101‑1 (MQ=17)
                                                    aTCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGc                                                           >  1:632608/1‑101 (MQ=255)
                                                    aTCGGTCGGATCGGTGGGATCGGTCGGGGCCGTGGGACCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggat                                                           <  1:1975970/101‑3 (MQ=14)
                                                      cggtcggGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTcgggtcc                                                         >  2:3340704/1‑98 (MQ=11)
                                                       ggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCGTcggatcgg                                                        >  1:1089415/1‑96 (MQ=14)
                                                        gtggggtCCGTGGGATCGGTCGGGTCCGTAGGATCGGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtggt                                                       <  1:2018791/95‑1 (MQ=255)
                                                        gtcggGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGAGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtggt                                                       >  1:3766127/1‑101 (MQ=255)
                                                          gcggtCCGTGGGGTCGGTCGGGTCCGTAGGATCCGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCtggtgg                                                     <  2:3589644/97‑1 (MQ=255)
                                                           ggATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggatcggtcgg                                                    <  2:1548767/101‑10 (MQ=11)
                                                           ggATCCGTGGGAACGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGGCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTgggaacggtcgg                                                    >  1:3187348/1‑92 (MQ=11)
                                                            gATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTaggatcggtcggg                                                   >  1:1162326/1‑91 (MQ=11)
                                                             gtCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAt                                                  <  2:3987409/100‑1 (MQ=255)
                                                             gtCCGTCGGATCCGTCGGATCGGTCGGATCCGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGAt                                                  <  2:494608/100‑1 (MQ=14)
                                                                      ttcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGAc                                         <  2:3020501/100‑1 (MQ=255)
                                                                      aTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGGTGGGTCCGTGGGCTCGGTGGGGTCAGGGGGACCGCTGGGaaccggtgata                                         >  2:2851210/1‑91 (MQ=0)
                                                                       tcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGAcc                                        <  1:1701155/101‑1 (MQ=255)
                                                                          ggcggGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCt                                     <  1:3873868/99‑1 (MQ=255)
                                                                             gggTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTc                                  >  1:3588800/1‑101 (MQ=255)
                                                                                tCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgcc                               >  2:1717757/1‑101 (MQ=255)
                                                                                   gTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTcgccgcc                            >  1:2921076/1‑101 (MQ=255)
                                                                                     aGGATCGGTCGGGTCCGTCGGGCCCGGGGGATCGGTCGGATCCGTGGGATGGGTCGGGTCCGTGGGGCGGGGGGGGTGGGTGGGGCCCCGGCcgccgccgc                          >  1:1535456/1‑101 (MQ=18)
                                                                                      ggATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCgccgccgcc                         >  2:2228276/1‑101 (MQ=255)
                                                                                       gATCGGTCGGGTCCGTCGGGTCCGTGGGGTCGGTCGGATCCGTGGGATCGGTCGGGTCCGCGGGGCTGGTGGGATCGGTCGGACCGCTGCCGacgccgccg                        >  2:2482322/1‑101 (MQ=25)
                                                                                            gtcgggtccgtcgggtccGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAg                   <  1:1417490/101‑1 (MQ=255)
                                                                                            gtcgggtccgtcgggtccGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAg                   >  2:1092386/1‑101 (MQ=255)
                                                                                                      tcgggtccttGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAggccg         >  1:2766618/1‑101 (MQ=255)
                                                                                                      gcgggtccgtGGGATCGGGCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAggccg         <  2:1587807/100‑1 (MQ=255)
                                                                                                       cgggtccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAggccgg        >  1:4273750/1‑101 (MQ=255)
                                                                                                           tccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAg    <  1:2109882/101‑1 (MQ=255)
                                                                                                           tccgtGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAg    <  1:342776/101‑1 (MQ=255)
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CGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGCTGGTGGGATCGGTCGGACCGCTGTCGCCGCCGCCGGACAGACCCAGGCCGGCCAGAC  >  NC_014034/221978‑222187

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG