Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,316 A→C 8.0% intergenic (‑112/‑193) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3160A→C 8.0% 30.8 ‑1.8 ‑0.0174intergenic (‑112/‑193)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (4/10):  ref base (78/82):  total (82/92)
Fisher's exact test strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.75e-01

Only 100 of 180 total aligned reads displayed.

CATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGAC  >  NC_014034/2249217‑2249414
                                                                                                       |                                                                                                      
 aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAg                                                                                                       <  2:1410844/101‑1 (MQ=255)
 aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAg                                                                                                       >  1:1451835/1‑101 (MQ=255)
 aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAg                                                                                                       <  1:473005/101‑1 (MQ=255)
  ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                                      >  2:3488609/1‑101 (MQ=255)
  ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                                      >  2:1806592/1‑101 (MQ=255)
  ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCCAGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                                      >  2:1216471/1‑101 (MQ=255)
   tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                                     <  2:4398909/101‑1 (MQ=255)
     gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCaa                                                                                                   >  1:4231972/1‑101 (MQ=255)
      tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAg                                                                                                  <  2:1131436/101‑1 (MQ=255)
      tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACC.CCCCCGGGATATTTGAGGCAAg                                                                                                  >  1:1343939/1‑101 (MQ=255)
      tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGCGACT.CCCCCGGGATATTTGAGGCAAg                                                                                                  >  2:732493/1‑101 (MQ=255)
       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                                 <  2:2353235/101‑1 (MQ=255)
         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                               <  1:4240242/101‑1 (MQ=255)
         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                               >  1:3338921/1‑101 (MQ=255)
          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                              <  1:3945259/101‑1 (MQ=255)
           ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                                             <  1:2292102/101‑1 (MQ=255)
           ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                                             <  2:272470/101‑1 (MQ=255)
              agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggg                                                                                       >  2:4263275/1‑101 (MQ=255)
              agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggg                                                                                       >  2:83736/1‑101 (MQ=255)
                aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggggg                                                                                     <  2:1664855/101‑1 (MQ=255)
                 tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggggg                                                                                    <  1:3768299/101‑1 (MQ=255)
                  gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGc                                                                                   <  1:431239/101‑1 (MQ=255)
                   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCt                                                                                  <  2:2499306/101‑1 (MQ=255)
                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                                <  1:896766/101‑1 (MQ=255)
                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                                <  2:3655832/101‑1 (MQ=255)
                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                                               <  1:4216614/101‑1 (MQ=255)
                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                                               <  1:1594615/101‑1 (MQ=255)
                       gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCa                                                                              >  2:2338390/1‑101 (MQ=255)
                       gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCa                                                                              >  1:174611/1‑101 (MQ=255)
                        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCACGATGAA...GGGGGGCTTTCAt                                                                             >  1:2654751/1‑101 (MQ=255)
                        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCAt                                                                             >  2:1026100/1‑101 (MQ=255)
                         tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATc                                                                            >  2:1570295/1‑101 (MQ=255)
                          aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                                           >  1:588565/1‑101 (MQ=255)
                          aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                                           >  1:907763/1‑101 (MQ=255)
                            cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTg                                                                         <  2:1451728/101‑1 (MQ=255)
                              cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGcc                                                                       >  2:1457976/1‑101 (MQ=255)
                               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCg                                                                      <  2:905584/101‑1 (MQ=255)
                               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCg                                                                      >  1:239901/1‑101 (MQ=255)
                               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCg                                                                      <  2:4228665/101‑1 (MQ=255)
                                gCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGa                                                                     <  2:2066088/101‑1 (MQ=255)
                                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                                    <  1:758475/101‑1 (MQ=255)
                                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                                    <  1:2927752/101‑1 (MQ=255)
                                  ttGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaaa                                                                   <  2:4225319/101‑1 (MQ=255)
                                  ttGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaaa                                                                   >  2:1538005/1‑101 (MQ=255)
                                    gggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                                 >  1:1337162/1‑101 (MQ=255)
                                    gggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                                 <  2:4468/101‑1 (MQ=255)
                                     ggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAata                                                                <  1:3156191/101‑1 (MQ=255)
                                       cACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATc                                                              <  2:2180251/101‑1 (MQ=255)
                                       cACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATc                                                              <  2:3982962/101‑1 (MQ=255)
                                        aCCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATcc                                                             <  1:2418784/101‑1 (MQ=255)
                                           cGGGGCAAGAAGGGGGCTCT.GCCCCC.GTGCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgg                                                          <  2:4371622/101‑1 (MQ=255)
                                           cGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgg                                                          >  1:1909028/1‑101 (MQ=255)
                                           cGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgg                                                          <  1:3170951/101‑1 (MQ=255)
                                              ggCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCggggg                                                       >  2:4366761/1‑101 (MQ=255)
                                               gCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                                      <  2:3245345/101‑1 (MQ=255)
                                               gCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                                      <  1:2587984/101‑1 (MQ=255)
                                                  agaggGGGCCTCT.CCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                   <  1:2338540/101‑1 (MQ=37)
                                                    aagGGGGCTCTCC.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                 <  2:951015/101‑1 (MQ=255)
                                                    aagGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAAt                                                 >  1:3570651/1‑101 (MQ=255)
                                                      gggggCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATgg                                               <  1:513595/101‑1 (MQ=255)
                                                       ggggCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                              <  1:69665/101‑1 (MQ=255)
                                                          gCTCT.GCCCCC.GTCCGGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGTCTTTTCATGTTGCCGAAACTATCCCGGTCGCTGAATGgcggc                                           >  2:295579/1‑99 (MQ=21)
                                                          gCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCa                                           <  2:3436257/101‑1 (MQ=255)
                                                          gCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCa                                           <  1:1006474/101‑1 (MQ=255)
                                                           ctct.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAg                                          >  2:247635/1‑101 (MQ=255)
                                                             tctgccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATTAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGc                                         >  1:2175671/6‑101 (MQ=255)
                                                              ctaccccc.GTCCCGTGCCGGGGCT.CCCCCGGGATATTTGATGCAAGATGAA...GGGGGGGTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                                        <  1:51823/97‑1 (MQ=37)
                                                                   ccc.ggcccgtGCCGGGACT.CCCCGGGGATATTTGAGGCAAGATGAA...GGGGGGGTTTCATCTTGCCGAAAATATCGCGGGGGGTGAATGGCGCAGCCAGAgg                                   <  1:2938630/101‑1 (MQ=38)
                                                                        tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggggg                               >  2:2576331/1‑101 (MQ=255)
                                                                         cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc                              >  1:2453951/1‑101 (MQ=255)
                                                                          ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa                             <  1:3924492/101‑1 (MQ=255)
                                                                           cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg                            <  2:1010498/101‑1 (MQ=255)
                                                                           cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAggggg                                <  2:272454/94‑1 (MQ=18)
                                                                           cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAggggg                                >  2:1957836/8‑101 (MQ=21)
                                                                            gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAATGGCGAAGCCAGAgggggg                               >  2:744283/7‑101 (MQ=18)
                                                                             caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAGGGGGGc                              <  1:2900124/96‑1 (MQ=18)
                                                                             caagcggACTCCCCCCGGGATATTGGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAGGGGGGc                              <  1:3882924/96‑1 (MQ=18)
                                                                                 gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccc                     >  2:508026/1‑101 (MQ=255)
                                                                                 gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccc                     >  2:630121/1‑101 (MQ=255)
                                                                                 gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccc                     >  2:110774/1‑101 (MQ=255)
                                                                                 gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccc                     >  1:421189/1‑101 (MQ=255)
                                                                                  ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccccc                    >  1:4337870/1‑101 (MQ=255)
                                                                                    gcT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccccct                  <  2:2654579/100‑3 (MQ=255)
                                                                                    actccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAGGGGGGCAGCG.ccc                      <  1:2070380/97‑1 (MQ=17)
                                                                                    aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccccct                  <  2:3414293/101‑3 (MQ=255)
                                                                                    aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccccct                  >  1:3225093/1‑99 (MQ=255)
                                                                                     cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccccctt                 >  2:1921007/1‑98 (MQ=255)
                                                                                      t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccccccttc                >  1:1825755/1‑97 (MQ=255)
                                                                                      t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccccccttc                >  2:594067/1‑97 (MQ=255)
                                                                                        cccccGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccccccttcg               <  2:3163756/101‑6 (MQ=255)
                                                                                          cccGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTcgcg              >  1:4389585/1‑101 (MQ=255)
                                                                                           ccGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCgcgg             >  1:1603494/1‑101 (MQ=255)
                                                                                            cGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCgcggg            >  2:1215378/1‑101 (MQ=255)
                                                                                             gggATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCgcgggc           >  1:3009782/1‑101 (MQ=255)
                                                                                             gggATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCgcgggc           >  1:954626/1‑101 (MQ=255)
                                                                                              ggATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCgcgggcg          >  1:3601854/1‑101 (MQ=255)
                                                                                               gATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCACGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCgccggcgg         >  1:3619563/1‑101 (MQ=25)
                                                                                                atgtttGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCGCGggcggc        <  2:1725741/98‑1 (MQ=255)
                                                                                                  atTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcg      <  1:1026188/101‑1 (MQ=255)
                                                                                                    ttGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAATGGCGCAGCCAGAGGGGGGTAGCGCCCCCCTCCGCGCGCGGCcgcc    >  2:1098390/1‑100 (MQ=25)
                                                                                                       |                                                                                                      
CATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGAC  >  NC_014034/2249217‑2249414

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG