Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,317 G→A 5.9% intergenic (‑113/‑192) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3170G→A 5.9% 19.3 ‑2.2 ‑0.3169intergenic (‑113/‑192)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (2/8):  ref base (76/82):  total (78/91)
Fisher's exact test strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.26e-01

Only 100 of 180 total aligned reads displayed.

ATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGC  >  NC_014034/2249218‑2249416
                                                                                                       |                                                                                                       
aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAg                                                                                                         >  1:1451835/1‑101 (MQ=255)
aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAg                                                                                                         <  1:473005/101‑1 (MQ=255)
 ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                                        >  2:1806592/1‑101 (MQ=255)
 ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                                        >  2:3488609/1‑101 (MQ=255)
 ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCCAGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                                        >  2:1216471/1‑101 (MQ=255)
  tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                                       <  2:4398909/101‑1 (MQ=255)
    gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCaa                                                                                                     >  1:4231972/1‑101 (MQ=255)
     tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACC.CCCCCGGGATATTTGAGGCAAg                                                                                                    >  1:1343939/1‑101 (MQ=255)
     tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGCGACT.CCCCCGGGATATTTGAGGCAAg                                                                                                    >  2:732493/1‑101 (MQ=255)
      gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                                   <  2:2353235/101‑1 (MQ=255)
        tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                 >  1:3338921/1‑101 (MQ=255)
        tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                                 <  1:4240242/101‑1 (MQ=255)
         cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                                                <  1:3945259/101‑1 (MQ=255)
          ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                                               <  2:272470/101‑1 (MQ=255)
          ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                                               <  1:2292102/101‑1 (MQ=255)
             agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggg                                                                                         >  2:4263275/1‑101 (MQ=255)
             agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggg                                                                                         >  2:83736/1‑101 (MQ=255)
               aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggggg                                                                                       <  2:1664855/101‑1 (MQ=255)
                tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggggg                                                                                      <  1:3768299/101‑1 (MQ=255)
                 gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGc                                                                                     <  1:431239/101‑1 (MQ=255)
                  cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCt                                                                                    <  2:2499306/101‑1 (MQ=255)
                    caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                                  <  1:896766/101‑1 (MQ=255)
                    caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                                  <  2:3655832/101‑1 (MQ=255)
                     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                                                 <  1:1594615/101‑1 (MQ=255)
                     aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                                                 <  1:4216614/101‑1 (MQ=255)
                      gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCa                                                                                >  2:2338390/1‑101 (MQ=255)
                      gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCa                                                                                >  1:174611/1‑101 (MQ=255)
                       aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCACGATGAA...GGGGGGCTTTCAt                                                                               >  1:2654751/1‑101 (MQ=255)
                       aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCAt                                                                               >  2:1026100/1‑101 (MQ=255)
                        tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATc                                                                              >  2:1570295/1‑101 (MQ=255)
                         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                                             >  1:907763/1‑101 (MQ=255)
                         aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                                             >  1:588565/1‑101 (MQ=255)
                           cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTg                                                                           <  2:1451728/101‑1 (MQ=255)
                             cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGcc                                                                         >  2:1457976/1‑101 (MQ=255)
                              aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCg                                                                        <  2:4228665/101‑1 (MQ=255)
                              aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCg                                                                        >  1:239901/1‑101 (MQ=255)
                              aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCg                                                                        <  2:905584/101‑1 (MQ=255)
                               gCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGa                                                                       <  2:2066088/101‑1 (MQ=255)
                                cTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                                      <  1:2927752/101‑1 (MQ=255)
                                cTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                                      <  1:758475/101‑1 (MQ=255)
                                 ttGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaaa                                                                     >  2:1538005/1‑101 (MQ=255)
                                 ttGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaaa                                                                     <  2:4225319/101‑1 (MQ=255)
                                  tGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaaaa                                                                    <  2:1298623/101‑1 (MQ=255)
                                   gggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                                   >  1:1337162/1‑101 (MQ=255)
                                   gggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                                   <  2:4468/101‑1 (MQ=255)
                                    ggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAata                                                                  <  1:3156191/101‑1 (MQ=255)
                                      cACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATc                                                                <  2:2180251/101‑1 (MQ=255)
                                      cACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATc                                                                <  2:3982962/101‑1 (MQ=255)
                                       aCCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATcc                                                               <  1:2418784/101‑1 (MQ=255)
                                          cGGGGCAAGAAGGGGGCTCT.GCCCCC.GTGCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgg                                                            <  2:4371622/101‑1 (MQ=255)
                                          cGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgg                                                            >  1:1909028/1‑101 (MQ=255)
                                          cGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgg                                                            <  1:3170951/101‑1 (MQ=255)
                                             ggCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCggggg                                                         >  2:4366761/1‑101 (MQ=255)
                                              gCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                                        >  2:1158809/1‑101 (MQ=255)
                                              gCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                                        <  2:3245345/101‑1 (MQ=255)
                                              gCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                                        <  1:2587984/101‑1 (MQ=255)
                                                 agaggGGGCCTCT.CCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                     <  1:2338540/101‑1 (MQ=37)
                                                   aagGGGGCTCTCC.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                                   <  2:951015/101‑1 (MQ=255)
                                                   aagGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAAt                                                   >  1:3570651/1‑101 (MQ=255)
                                                     gggggCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATgg                                                 <  1:513595/101‑1 (MQ=255)
                                                      ggggCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                                                <  1:69665/101‑1 (MQ=255)
                                                         gCTCT.GCCCCC.GTCCGGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGTCTTTTCATGTTGCCGAAACTATCCCGGTCGCTGAATGgcggc                                             >  2:295579/1‑99 (MQ=21)
                                                         gCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCa                                             <  2:3436257/101‑1 (MQ=255)
                                                         gCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCa                                             <  1:1006474/101‑1 (MQ=255)
                                                          ctct.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAg                                            >  2:247635/1‑101 (MQ=255)
                                                            tctgccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATTAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGc                                           >  1:2175671/6‑101 (MQ=255)
                                                             ctaccccc.GTCCCGTGCCGGGGCT.CCCCCGGGATATTTGATGCAAGATGAA...GGGGGGGTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                                          <  1:51823/97‑1 (MQ=37)
                                                                  ccc.ggcccgtGCCGGGACT.CCCCGGGGATATTTGAGGCAAGATGAA...GGGGGGGTTTCATCTTGCCGAAAATATCGCGGGGGGTGAATGGCGCAGCCAGAgg                                     <  1:2938630/101‑1 (MQ=38)
                                                                       tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggggg                                 >  2:2576331/1‑101 (MQ=255)
                                                                        cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc                                >  1:2453951/1‑101 (MQ=255)
                                                                         ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa                               <  1:3924492/101‑1 (MQ=255)
                                                                          cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg                              <  2:1010498/101‑1 (MQ=255)
                                                                          cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAggggg                                  <  2:272454/94‑1 (MQ=18)
                                                                          cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAggggg                                  >  2:1957836/8‑101 (MQ=21)
                                                                           gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAATGGCGAAGCCAGAgggggg                                 >  2:744283/7‑101 (MQ=18)
                                                                            caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAGGGGGGc                                <  1:2900124/96‑1 (MQ=18)
                                                                            caagcggACTCCCCCCGGGATATTGGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAGGGGGGc                                <  1:3882924/96‑1 (MQ=18)
                                                                                gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccc                       >  2:508026/1‑101 (MQ=255)
                                                                                gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccc                       >  1:421189/1‑101 (MQ=255)
                                                                                gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccc                       >  2:630121/1‑101 (MQ=255)
                                                                                 ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccccc                      >  1:4337870/1‑101 (MQ=255)
                                                                                   gcT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccccct                    <  2:2654579/100‑3 (MQ=255)
                                                                                   actccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAGGGGGGCAGCG.ccc                        <  1:2070380/97‑1 (MQ=17)
                                                                                   aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccccct                    <  2:3414293/101‑3 (MQ=255)
                                                                                   aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccccct                    >  1:3225093/1‑99 (MQ=255)
                                                                                    cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.cccccctt                   >  2:1921007/1‑98 (MQ=255)
                                                                                    actacgccGGGATATTTGGGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATCTCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCT.cccccct                    <  2:1125168/95‑3 (MQ=25)
                                                                                     t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccccccttc                  >  2:594067/1‑97 (MQ=255)
                                                                                     t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccccccttc                  >  1:1825755/1‑97 (MQ=255)
                                                                                       cccccGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.ccccccttcg                 <  2:3163756/101‑6 (MQ=255)
                                                                                         cccGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTcgcg                >  1:4389585/1‑101 (MQ=255)
                                                                                          ccGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCgcgg               >  1:1603494/1‑101 (MQ=255)
                                                                                            gggATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCgcgggc             >  1:3009782/1‑101 (MQ=255)
                                                                                            gggATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCgcgggc             >  1:954626/1‑101 (MQ=255)
                                                                                             ggATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCgcgggcg            >  1:3601854/1‑101 (MQ=255)
                                                                                              gATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCACGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCgccggcgg           >  1:3619563/1‑101 (MQ=25)
                                                                                               atgtttGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCGCGggcggc          <  2:1725741/98‑1 (MQ=255)
                                                                                                 atTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcg        <  1:1026188/101‑1 (MQ=255)
                                                                                                   ttGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAATGGCGCAGCCAGAGGGGGGTAGCGCCCCCCTCCGCGCGCGGCcgcc      >  2:1098390/1‑100 (MQ=25)
                                                                                                       ggCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcgc  <  2:144875/101‑1 (MQ=255)
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ATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGC  >  NC_014034/2249218‑2249416

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG