Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,347 G→C 9.5% intergenic (‑143/‑162) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3470G→C 9.5% 29.8 ‑1.8 ‑1.5147intergenic (‑143/‑162)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (4/10):  ref base (67/66):  total (71/76)
Fisher's exact test strand distribution p-value = 1.62e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.21e-02

Only 100 of 163 total aligned reads displayed.

AGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAT  >  NC_014034/2249248‑2249446
                                                                                                           |                                                                                                         
aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                                                           >  1:3377750/1‑101 (MQ=255)
aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                                                           <  2:905584/101‑1 (MQ=255)
aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                                                           <  2:4228665/101‑1 (MQ=255)
aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                                                           >  1:239901/1‑101 (MQ=255)
  cTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                                         <  1:758475/101‑1 (MQ=255)
   ttGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaa                                                                                                        <  2:4225319/101‑1 (MQ=255)
    tGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                                       <  1:986743/101‑1 (MQ=255)
    tGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                                       <  2:1298623/101‑1 (MQ=255)
     gggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                                      >  1:1337162/1‑101 (MQ=255)
     gggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                                      <  2:4468/101‑1 (MQ=255)
      ggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAata                                                                                                     <  1:3156191/101‑1 (MQ=255)
        cACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                                   <  2:2180251/101‑1 (MQ=255)
        cACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                                   >  2:3232114/1‑101 (MQ=255)
        cACCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                                   <  2:3982962/101‑1 (MQ=255)
         aCCCGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATcc                                                                                                  <  1:2418784/101‑1 (MQ=255)
           ccGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                                                >  1:4321817/1‑101 (MQ=255)
            cGGGGCAAGAAGGGGGCTCT.GCCCCCGTGCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                                               <  2:4371622/101‑1 (MQ=255)
            cGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                                               <  1:3170951/101‑1 (MQ=255)
            cGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                                               >  2:3468556/1‑101 (MQ=255)
                gCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggg                                                                                           >  2:1158809/1‑101 (MQ=255)
                   agaggGGGCCTCT.CCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                                        <  1:2338540/101‑1 (MQ=37)
                     aagGGGGCTCTCC.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                                     <  2:951015/101‑1 (MQ=255)
                     aagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.t                                                                                     >  1:3570651/1‑101 (MQ=255)
                      agGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                    <  2:1526650/101‑1 (MQ=255)
                        ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                                  <  1:69665/101‑1 (MQ=255)
                           gCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                               <  1:1006474/101‑1 (MQ=255)
                            ctct.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                                            >  2:247635/1‑101 (MQ=255)
                              tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATTAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gc                                                                           >  1:2175671/6‑101 (MQ=255)
                               ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                          >  1:258922/5‑101 (MQ=255)
                               ctacccccGTCCCGTGCCGGGGCT.CCCCCGGGATATTTGATGCAAGATGAA...GGGG..GGGTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                          <  1:51823/97‑1 (MQ=37)
                                  cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                                                       <  1:4090986/100‑1 (MQ=255)
                                    cccggcccgtGCCGGGACT.CCCCGGGGATATTTGAGGCAAGATGAA...GGGG..GGGTTTCATCTTGCCGAAAATATCGCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                                                     <  1:2938630/101‑1 (MQ=38)
                                     ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                    >  2:929917/1‑101 (MQ=255)
                                        tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                 >  2:2576331/1‑101 (MQ=255)
                                          ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                               <  1:3924492/101‑1 (MQ=255)
                                          ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                               <  2:4291910/101‑1 (MQ=255)
                                           cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                              <  2:1010498/101‑1 (MQ=255)
                                           cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                                  <  2:3398244/94‑1 (MQ=18)
                                           cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                                  <  1:1337573/94‑1 (MQ=18)
                                            gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGCGAA..GCCAGAgggggg                                                                 >  2:744283/7‑101 (MQ=18)
                                             caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                                <  1:2900124/96‑1 (MQ=18)
                                             caagcggACTCCCCCCGGGATATTGGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                                                <  1:3882924/96‑1 (MQ=18)
                                              gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c.g.c                                                        >  2:2978001/1‑101 (MQ=255)
                                               ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cc                                                       <  1:499750/101‑1 (MQ=255)
                                                cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccc                                                      >  1:515202/1‑101 (MQ=255)
                                                 gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                     >  2:508026/1‑101 (MQ=255)
                                                 gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                     >  2:630121/1‑101 (MQ=255)
                                                 gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                     >  2:110774/1‑101 (MQ=255)
                                                 gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                     >  1:421189/1‑101 (MQ=255)
                                                 cggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAg.c.g                                                          <  2:2752959/100‑1 (MQ=18)
                                                  ggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccc                                                    >  1:4337870/1‑101 (MQ=255)
                                                   gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccc                                                   <  2:2566420/101‑2 (MQ=255)
                                                    actccccccGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAG.C.G.ccc                                                      <  1:2070380/97‑1 (MQ=17)
                                                    aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccct                                                  >  1:3225093/1‑99 (MQ=255)
                                                    aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccct                                                  <  1:2906216/101‑3 (MQ=255)
                                                    aCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccct                                                  <  2:3414293/101‑3 (MQ=255)
                                                     cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccccctt                                                 >  2:1921007/1‑98 (MQ=255)
                                                      t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttc                                                >  2:594067/1‑97 (MQ=255)
                                                       ccccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.ccccc                                                    <  2:2095384/100‑1 (MQ=0)
                                                        cccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCCCCt                                                   <  1:3122854/101‑1 (MQ=0)
                                                        cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttcg                                               <  2:3163756/101‑6 (MQ=255)
                                                         ccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCCCCtg                                                  >  2:670123/1‑100 (MQ=2)
                                                         ccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTcgc                                               <  2:1700602/101‑1 (MQ=255)
                                                           ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgg                                             >  1:1603494/1‑101 (MQ=255)
                                                            cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCCCCtgccc                                               >  2:2044605/1‑97 (MQ=2)
                                                             gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgggc                                           >  1:954626/1‑101 (MQ=255)
                                                             gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgggc                                           >  2:2718678/1‑101 (MQ=255)
                                                               gATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCACGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgccggcgg                                         >  1:3619563/1‑101 (MQ=25)
                                                                atgtttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGggcggc                                        <  2:1725741/98‑1 (MQ=255)
                                                                  atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCcg                                      <  1:1026188/101‑1 (MQ=255)
                                                                    ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGTAG.C.GCCCCCCTCCGCGCGCGGCcgcc                                    >  2:1098390/1‑100 (MQ=25)
                                                                      gAGGCAAGATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAc                                  <  2:1451519/101‑1 (MQ=37)
                                                                          cAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgc                              >  2:2892422/1‑101 (MQ=255)
                                                                           aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgcg                             >  2:434029/1‑101 (MQ=255)
                                                                           aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgcg                             >  2:1480789/1‑101 (MQ=255)
                                                                           aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgcgcg                             >  2:1578778/1‑101 (MQ=255)
                                                                             gaggAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc                           <  2:1825631/98‑1 (MQ=255)
                                                                              aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcggcg                          >  2:539962/1‑101 (MQ=255)
                                                                              aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgccg                          <  1:2627658/101‑1 (MQ=255)
                                                                              aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGAGcgcct                          >  2:4391130/1‑100 (MQ=255)
                                                                               tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgccgc                         >  2:2393415/1‑101 (MQ=255)
                                                                               tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgccgc                         >  2:4191414/1‑101 (MQ=255)
                                                                                gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CAC.CCCCCTTCGCGGGCGACCGCGACGCGCGCCGCa                        >  2:4016059/1‑101 (MQ=37)
                                                                                gAA...GGGG..GGCTTTCATATTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa                        >  2:485481/1‑101 (MQ=255)
                                                                                 aggaggggg..gcCTTTCATCTGGCCGAAAATTTCCGGGGGGGGGAA.TGGCGCA..GCCAGGGGGGGGCAGCC.C.CCCCCTTCGCGGGCGGCCGCGACGCGcgccg                          <  1:1626833/96‑1 (MQ=255)
                                                                                 aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa                       <  2:1254047/101‑1 (MQ=255)
                                                                                  a...GGGG..GGCTTTCATCTTGCGGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                      <  2:1976457/101‑1 (MQ=255)
                                                                                  a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                      <  1:1887955/101‑1 (MQ=255)
                                                                                  a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                      >  2:1113979/1‑101 (MQ=255)
                                                                                  a...GGGG..GCCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                      <  2:4321548/101‑1 (MQ=255)
                                                                                       ggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                    >  1:1937042/1‑101 (MQ=255)
                                                                                            ggCTTTCATCTTGCCAAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCC.C.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                 <  1:83749/101‑1 (MQ=38)
                                                                                               tttCATCTTGCCAAAAATGGCCGGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg              <  1:956221/101‑1 (MQ=37)
                                                                                                  cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc           <  2:180444/101‑1 (MQ=255)
                                                                                                     tttGCCGAAAATATCCCGGGGGGTGAA.GGGCGCA..GCCAGAGGGGGGCGG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt        <  1:2463749/100‑1 (MQ=37)
                                                                                                     cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt        <  2:239874/101‑1 (MQ=255)
                                                                                                      ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt       >  2:477158/1‑101 (MQ=255)
                                                                                                      ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt       <  1:1921131/101‑1 (MQ=255)
                                                                                                       tGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg      >  2:231073/1‑101 (MQ=255)
                                                                                                           gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt  >  1:1560187/1‑101 (MQ=255)
                                                                                                           |                                                                                                         
AGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAT  >  NC_014034/2249248‑2249446

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG