BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,254,791	G→A	8.5%	F25F (TTC→TTT)	RCAP_rcc02086 ←	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,254,791	0	G→A	8.5%	51.0 ‑1.3 ‑0.9	246	F25F (TTC→TTT)	RCAP_rcc02086	hypothetical protein
Reads supporting (aligned to +/- strand): new base (13/8): ref base (109/116): total (122/124)
Fisher's exact test strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.30e-01

Only 100 of 259 total aligned reads displayed.


CCGCGACGCTGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAT  >  NC_014034/2254691‑2254891


   cgACGCTGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAg                                                                                                   <  1:3449873/101‑1 (MQ=255)


   cgACGCTGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAg                                                                                                   >  1:3085317/1‑101 (MQ=255)


   cgACGCTGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAg                                                                                                   <  1:3888609/101‑1 (MQ=255)


   cgACGCTGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAg                                                                                                   <  1:2264032/101‑1 (MQ=255)


    gACGCTGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTTATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAgg                                                                                                  >  1:4409884/1‑101 (MQ=255)


    gACGCTGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAgg                                                                                                  >  2:3215406/1‑101 (MQ=255)


    gACGCTGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAgg                                                                                                  <  1:3224947/101‑1 (MQ=255)


       gCTGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCgg                                                                                               <  2:2243687/101‑2 (MQ=255)


        cTGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTc                                                                                              >  2:3692596/1‑101 (MQ=255)


        cTGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTc                                                                                              >  2:1564502/1‑101 (MQ=255)


        cTGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTc                                                                                              <  1:4323920/101‑1 (MQ=255)


         tGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCa                                                                                             <  1:1723534/101‑1 (MQ=255)


         tGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCa                                                                                             <  1:1799904/101‑1 (MQ=255)


         tGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCa                                                                                             <  1:1413000/101‑1 (MQ=255)


          ggCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCaa                                                                                            >  2:872207/1‑101 (MQ=255)


           gcgcCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAg                                                                                           <  2:932218/101‑1 (MQ=255)


             ggcGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGt                                                                                         <  2:556279/99‑1 (MQ=255)


             gcCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGt                                                                                         <  1:2404666/101‑1 (MQ=255)


              ccGGACGCCCGTCAACGGGGCCCCCCAAACAGGTGAGCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAAGGGGCAGCGCCGAGGCGAAGGCGTCAAGGTc                                                                                        <  2:1147475/101‑1 (MQ=255)


               cGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGAGTCAAGGTCg                                                                                       >  2:778307/1‑101 (MQ=255)


                 gACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGaa                                                                                     >  2:1798947/1‑101 (MQ=255)


                    gCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCg                                                                                  >  2:4424621/1‑101 (MQ=255)


                     cccTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCgg                                                                                 >  2:2603/1‑101 (MQ=255)


                         tCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGt                                                                             <  2:4022721/101‑1 (MQ=255)


                           aaCTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTaa                                                                           <  2:4388753/101‑1 (MQ=255)


                           aaCTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTaa                                                                           >  1:3538049/1‑101 (MQ=255)


                           aaCTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTaa                                                                           <  1:492278/101‑1 (MQ=255)


                           aaCTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTaa                                                                           <  2:2674160/101‑1 (MQ=255)


                           aaCTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTaa                                                                           <  2:794456/101‑1 (MQ=255)


                             cTGGACCCCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAg                                                                         <  1:358523/101‑1 (MQ=255)


                                  cacCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGtt                                                                    <  1:1577476/101‑1 (MQ=255)


                                   cccccAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGttt                                                                   <  2:1958579/100‑1 (MQ=255)


                                   acCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGttt                                                                   >  1:2753860/1‑101 (MQ=255)


                                    ccccAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTc                                                                  <  1:4185597/101‑1 (MQ=255)


                                      ccAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGc                                                                >  2:3016763/1‑101 (MQ=255)


                                          aCAGGTGCTCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGccccc                                                            <  2:4264981/101‑1 (MQ=255)


                                          aCAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGccccc                                                            <  2:712755/101‑1 (MQ=255)


                                           cAGGTGATCGGATGCCGTTTCCCCTTCCCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCa                                                           <  2:2012299/101‑1 (MQ=255)


                                           cAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCa                                                           <  2:2290407/101‑1 (MQ=255)


                                           cAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCa                                                           >  1:1938423/1‑101 (MQ=255)


                                           cAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCa                                                           >  2:2588090/1‑101 (MQ=255)


                                            aGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAg                                                          <  2:3371563/101‑1 (MQ=255)


                                            aGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAg                                                          >  1:3795208/1‑101 (MQ=255)


                                              gTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCt                                                        <  2:2753670/101‑1 (MQ=255)


                                              gTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCt                                                        <  2:1591370/101‑1 (MQ=255)


                                               tGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCtt                                                       <  2:1662449/101‑1 (MQ=255)


                                                    gCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGgc                                                  >  2:2391396/1‑101 (MQ=255)


                                                      aTGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGgcgc                                                >  1:3774306/1‑101 (MQ=255)


                                                       tGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGcgcg                                               >  1:968217/1‑101 (MQ=255)


                                                         ccGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCggg                                             >  2:2268762/1‑101 (MQ=255)


                                                           gTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAt                                           >  1:1252915/1‑101 (MQ=255)


                                                            tttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtc                                          >  2:4143909/1‑101 (MQ=255)


                                                            tttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtc                                          >  1:3722219/1‑101 (MQ=255)


                                                            tttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGAGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtc                                          <  1:778387/101‑1 (MQ=255)


                                                             ttCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGAtct                                         <  2:3162406/101‑1 (MQ=255)


                                                               ccccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTcc                                       >  1:914074/1‑101 (MQ=255)


                                                                gccTTCGCTCTCGCTCTCAATGGGCAGCGCCTAGGCGAAGGCGTCAAGGTCGAAGGGGGGGAAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCca                                      <  2:633213/100‑1 (MQ=255)


                                                                cccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCca                                      <  2:3059295/101‑1 (MQ=255)


                                                                 ccTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcag                                     >  2:249123/1‑101 (MQ=255)


                                                                  cgatcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgagg                                    >  2:1876050/9‑100 (MQ=255)


                                                                  cTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagc                                    <  2:2710918/101‑1 (MQ=255)


                                                                   ttCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCcagca                                   >  1:1485842/1‑101 (MQ=255)


                                                                    atcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggcc                                  <  2:887649/95‑4 (MQ=255)


                                                                    atcgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggcc                                  >  1:1842339/7‑98 (MQ=255)


                                                                     cGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGt                                 >  1:3116928/1‑101 (MQ=255)


                                                                      gCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTc                                <  2:1252011/101‑1 (MQ=255)


                                                                      cgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggctgc                                <  1:1052952/97‑6 (MQ=255)


                                                                      cgcgtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgc                                >  1:2154195/5‑96 (MQ=255)


                                                                        tctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGc                              >  2:1363121/1‑101 (MQ=255)


                                                                        tctcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGc                              <  2:599181/101‑1 (MQ=255)


                                                                         gtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggnngngcn                             >  1:1698863/2‑93 (MQ=255)


                                                                         gtcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgcgca                             >  1:1630658/2‑93 (MQ=255)


                                                                          tcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCaa                            <  2:1027982/101‑1 (MQ=255)


                                                                          tcGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCaa                            <  1:2304493/101‑1 (MQ=255)


                                                                          tcGCCCTCAATGGGCAGCGCCGAGGCAAAAGCGTCAAGGTCGTAGCGATCGTAGAGGCGCTTGGCTCCAAGCATCCGGCGCGGGATGTCgaggccgcgcag                            >  2:3757042/1‑92 (MQ=255)


                                                                             cccTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGt                         >  2:4092207/1‑101 (MQ=255)


                                                                               cTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGc                       >  1:2030520/1‑101 (MQ=255)


                                                                               cTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGCGc                       >  2:1659720/1‑101 (MQ=255)


                                                                                tCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCt                      >  2:3428464/1‑101 (MQ=255)


                                                                                  aaTGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGCTCTCCAGCAGTCGCAAGGTGCttt                    >  1:2481783/1‑101 (MQ=255)


                                                                                  aaTGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCttt                    <  1:2635416/101‑1 (MQ=255)


                                                                                    tGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCa                  >  2:4211810/1‑101 (MQ=255)


                                                                                    tGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCa                  <  2:2142690/101‑1 (MQ=255)


                                                                                    tGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCa                  >  2:2498939/1‑101 (MQ=255)


                                                                                     gggCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCag                 <  1:4002189/101‑1 (MQ=255)


                                                                                     gggCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCag                 <  1:3382059/101‑1 (MQ=255)


                                                                                      ggCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCaga                <  1:3695490/101‑1 (MQ=255)


                                                                                        cAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAgaga              <  1:2120721/101‑1 (MQ=255)


                                                                                        cAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAgaga              <  2:1174063/101‑1 (MQ=255)


                                                                                         aGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGAc             >  1:3043074/1‑101 (MQ=255)


                                                                                         aGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGGCGCAAGGTGCTTTCAGAGAc             >  1:3025739/1‑101 (MQ=255)


                                                                                            gcCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGcc          <  2:3471947/101‑1 (MQ=255)


                                                                                            gcCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGcc          >  1:2988200/1‑101 (MQ=255)


                                                                                              cGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCaa        <  1:249150/101‑1 (MQ=255)


                                                                                               gAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAg       >  1:1557211/1‑101 (MQ=255)


                                                                                               gAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAg       >  2:3950834/1‑101 (MQ=255)


                                                                                                   cGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTaa   <  1:2588261/101‑1 (MQ=255)


                                                                                                    gAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTaag  >  2:4287548/1‑100 (MQ=255)


                                                                                                    gAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAt  >  1:4108914/1‑101 (MQ=255)


                                                                                                    gAAGGCGTCAAGGGCGAAGCGGTCGTAAAGACGTTTGGCCCCCAGCTTTCGGCGCGGGATCTCTAGCAGTCGCAAGGTGTTTTCAGAGACGCCAAGGCaag  >  2:2938025/1‑100 (MQ=255)


CCGCGACGCTGGCGCCGGACGCCCTTCAACTGGACACCCCAAACAGGTGATCGCATGCCGTTTCCCCTTCGCTCTCGCCCTCAATGGGCAGCGCCGAGGCGAAGGCGTCAAGGTCGAAGCGGTCGTAAAGACGTTTCGCCCCCAGCTTTCGGCGCGGGATCTCCAGCAGTCGCAAGGTGCTTTCAGAGACGCCAAGGTAAT  >  NC_014034/2254691‑2254891

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG