Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,262,586 T→C 100% Q55R (CAG→CGG)  RCAP_rcc02105 ← IS3/IS911 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,262,5860T→C100.0% 741.9 206Q55R (CAG→CGG) RCAP_rcc02105IS3/IS911 family transposase
Reads supporting (aligned to +/- strand):  new base (123/82):  ref base (0/0):  total (123/83)

Only 100 of 242 total aligned reads displayed.

TTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGG  >  NC_014034/2262489‑2262683
                                                                                                 |                                                                                                 
ttGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAgccgct                                                                                                <  1:2586933/101‑1 (MQ=255)
  gggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTg                                                                                              <  2:1555239/101‑1 (MQ=255)
   ggCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTgg                                                                                             >  1:1961304/1‑101 (MQ=255)
     cTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCa                                                                                           >  2:4036024/1‑101 (MQ=255)
      ttGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCCGCCGCTTGGCAt                                                                                          <  1:2421541/101‑1 (MQ=255)
       tGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATc                                                                                         <  1:3123474/101‑1 (MQ=255)
             aaTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACaa                                                                                   <  2:2172619/101‑1 (MQ=255)
              aTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACaaa                                                                                  <  2:572367/101‑1 (MQ=255)
              aTGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACaaa                                                                                  <  1:1814194/101‑1 (MQ=255)
                   gcCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccat                                                                             <  1:2807150/101‑1 (MQ=255)
                     cacaCTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAatccatcc                                                                           <  2:3266432/101‑1 (MQ=255)
                      acacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATccc                                                                          >  1:3206370/1‑101 (MQ=255)
                       cccgggCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATcccg                                                                         <  2:3881624/97‑2 (MQ=255)
                       cacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATcccg                                                                         <  1:3683188/101‑2 (MQ=255)
                       cacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATcccg                                                                         <  1:672053/101‑2 (MQ=255)
                       cacTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATcccg                                                                         <  1:711373/101‑2 (MQ=255)
                         cTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcc                                                                       >  2:4311572/1‑101 (MQ=255)
                          tGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcca                                                                      >  1:3229914/1‑101 (MQ=255)
                          tGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcca                                                                      >  2:1268035/1‑101 (MQ=255)
                          nggncnnaTCCAGCATCACACCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCccgcca                                                                      <  1:1507716/94‑1 (MQ=18)
                           ggCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCAt                                                                     >  2:3785189/1‑101 (MQ=255)
                            gCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATa                                                                    >  2:290943/1‑101 (MQ=255)
                             cTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATAc                                                                   <  1:525640/101‑1 (MQ=255)
                              tCGATCCCGCATCCCACCCGCCCCCAGGCGCTTCAGCTCGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCCTCCCGCCATACt                                                                  <  2:1697801/101‑1 (MQ=18)
                                gATCCAGCATCCCACCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTg                                                                <  1:246865/101‑1 (MQ=18)
                                gATCCAGCATCACATCCGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAACCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTg                                                                <  1:3643622/101‑1 (MQ=18)
                                gATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATAGTTg                                                                >  2:2970937/1‑101 (MQ=18)
                                 aTCCAGCACACCCTCCGCCACCGGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACGCCTTCAGCCGCTGGGCATCGGACAAAGCCACCCCGCCATACTTgg                                                               <  2:1857955/101‑1 (MQ=255)
                                  tCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGc                                                              >  2:935751/1‑101 (MQ=255)
                                     accaccacaTCCCCCCCCAGGGCCTTCAGCTTGGCGTTCTCTTCCCCAAACTCCTTCAGCCGCTTGGCATCGGAAAAATCCACCCCGCCATACTTGGCCtt                                                           <  1:3925841/96‑1 (MQ=12)
                                      gCATCCCACCCGCCCCCAGGCGCTTCGGCTTGGCTTTTTCTTCATCAAGCTGCTTCAGCCGCTGGGCATCGGCCAAATCCATCCCGCCATACTTGGCCTTc                                                          <  2:3688802/101‑1 (MQ=12)
                                       cATCACACCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCa                                                         <  2:2584188/101‑1 (MQ=18)
                                        aTCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAg                                                        >  1:110156/1‑101 (MQ=255)
                                        aTCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAg                                                        >  2:3009900/1‑101 (MQ=255)
                                        aTCACACCCCCCCCCAGGCGCTTCAGCTTGCCTTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATGGGACAAATCCATCCCGCCATACTTGGCCTTCAg                                                        <  2:3089897/101‑1 (MQ=18)
                                           acaTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCtt                                                     <  2:395569/101‑1 (MQ=255)
                                            cacccGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTg                                                    <  1:4036281/98‑1 (MQ=18)
                                            caTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTg                                                    >  1:1107261/1‑101 (MQ=255)
                                             aTCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGt                                                   >  1:2335530/1‑101 (MQ=255)
                                              tCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCCTACTTGGCCTTCAGCTTGTa                                                  >  2:3594677/1‑101 (MQ=18)
                                              tCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTa                                                  >  1:287962/1‑101 (MQ=255)
                                              tCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTa                                                  >  2:952052/1‑101 (MQ=255)
                                               ccGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAg                                                 >  1:1625615/1‑101 (MQ=255)
                                                cGCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGa                                                <  1:1243689/101‑1 (MQ=18)
                                                 gCCCCCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGaa                                               <  1:1015573/101‑1 (MQ=18)
                                                 gCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGaa                                               >  1:576236/1‑101 (MQ=255)
                                                 cccaccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGaa                                               <  2:3427649/100‑1 (MQ=255)
                                                  ccaccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAg                                              >  2:745644/1‑101 (MQ=255)
                                                   caccaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAgg                                             >  1:348301/1‑101 (MQ=255)
                                                    cccaGGCGCTTCAGCTTGGCGTTCTCTCCCTCAAACTGCTTCAGCCGCTTGCCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGt                                            <  1:377345/100‑1 (MQ=18)
                                                    accaGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGt                                            >  2:4167083/1‑101 (MQ=255)
                                                      caGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcg                                          >  1:1389871/1‑101 (MQ=255)
                                                        ggCGCTTCAGCTTGGCGTTCTCTTCCTCAACCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcgcg                                        <  2:2861525/101‑1 (MQ=18)
                                                        ggCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCAGCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTcgcg                                        <  1:1556545/101‑1 (MQ=18)
                                                          cgcTTCAGCTTGGCGTTCTCTTCCTCAACCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCggg                                      <  2:3384993/101‑1 (MQ=18)
                                                           gcTTCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCgggg                                     >  1:1763291/1‑101 (MQ=255)
                                                             ttCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCt                                   <  1:2055341/101‑1 (MQ=255)
                                                             ttCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCt                                   >  1:2976253/1‑101 (MQ=255)
                                                             ttCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCt                                   >  1:803940/1‑101 (MQ=255)
                                                             ttCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCt                                   >  2:1400184/1‑101 (MQ=255)
                                                             ttCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCt                                   <  1:4444775/101‑1 (MQ=255)
                                                              tCAGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTg                                  <  2:348266/101‑1 (MQ=255)
                                                                aGCTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAg                                >  2:3372210/1‑101 (MQ=255)
                                                                  cTTGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGcc                              >  2:122454/1‑101 (MQ=255)
                                                                   ttGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGccc                             >  1:4314975/1‑101 (MQ=255)
                                                                    tGGCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCa                            >  2:2756300/1‑101 (MQ=255)
                                                                    tGGCGTTCGCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCa                            >  2:1665486/1‑101 (MQ=25)
                                                                     ggCGTTCCCTTCCCCAACCTGCTACACCCGCTTGGCAGCGGACAAATCAATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCAt                           <  2:2946701/101‑1 (MQ=21)
                                                                      gctttCTTTCCCTCAAACTCCTTCCGCTCTTTGGCACGGAACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATg                          <  2:2204022/98‑1 (MQ=16)
                                                                      gCGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATg                          <  2:4027870/101‑1 (MQ=25)
                                                                       cGTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGc                         >  1:4125952/1‑101 (MQ=255)
                                                                        gTTCTCTTCCTCAACCTGCTTCAGCGGCTTGGCATCGGAAAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCt                        <  2:1961175/101‑1 (MQ=21)
                                                                        gTTCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCt                        >  2:481703/1‑101 (MQ=255)
                                                                         ttCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCtt                       >  2:759307/1‑101 (MQ=255)
                                                                         ttCTCTTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCtt                       <  1:1207128/101‑1 (MQ=255)
                                                                           ctctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCttac                     <  1:290978/101‑3 (MQ=255)
                                                                            tctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCg                    >  1:3096028/1‑101 (MQ=37)
                                                                             ctTCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCgg                   >  1:3404442/1‑101 (MQ=255)
                                                                              ttCCTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGc                  >  2:4235385/1‑101 (MQ=25)
                                                                               tCCCCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCa                 <  2:3547095/101‑1 (MQ=25)
                                                                                ccTCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCaa                >  2:1042574/1‑101 (MQ=255)
                                                                                  tCAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAg              >  2:3169847/1‑101 (MQ=255)
                                                                                  ccAAGCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAg              <  1:2739785/100‑1 (MQ=21)
                                                                                   cAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGc             >  1:96754/1‑101 (MQ=255)
                                                                                   cAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGc             >  2:2505985/1‑101 (MQ=255)
                                                                                   cAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGc             <  1:4167344/101‑1 (MQ=255)
                                                                                   cAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGc             >  2:4109854/1‑101 (MQ=255)
                                                                                   cAAACTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGc             >  2:4063160/1‑101 (MQ=255)
                                                                                      aCTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCg          <  2:1625509/101‑1 (MQ=255)
                                                                                       cTGCTTCAGCCGCTTGGCATCGGGCAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCgg         >  2:2345523/1‑101 (MQ=21)
                                                                                       cTGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCgg         >  2:51836/1‑101 (MQ=255)
                                                                                        tGCTTCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGa        <  1:1329938/101‑1 (MQ=255)
                                                                                         gcttCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGaa       <  1:2573869/98‑1 (MQ=255)
                                                                                         gcttCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGaa       <  2:3206141/98‑1 (MQ=25)
                                                                                          cttCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAg      >  2:2642573/3‑101 (MQ=255)
                                                                                          cttCAGCCGCTTGGCATCGGACAAACCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAg      <  2:1509584/99‑1 (MQ=25)
                                                                                           ttCAGCCGCTTGGCATCGGACAAATCCACCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGt     <  2:3741218/100‑1 (MQ=255)
                                                                                            tCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTc    >  1:2269060/1‑101 (MQ=255)
                                                                                            tCAGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTc    <  2:2283981/101‑1 (MQ=255)
                                                                                              aGCCGCTTGGCATCGGACAAATCCATCCCGCCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCgg  >  1:3881984/1‑101 (MQ=255)
                                                                                                 |                                                                                                 
TTGGGCTTGAACCAATGGCGCCACACTGGCTCGATCCAGCATCACATCCGCCACCAGGCGCTTCAGCTTGGCGTTCTCTTCCTCAAACTGCGTCAGCTGCTTGGCATCGGACAAATCCATCCCACCATACTTGGCCTTCAGCTTGTAGAAGGTCGCGGGGCTGAGCCCATGCTTCCGGCAAAGCTCGGAAGTCGG  >  NC_014034/2262489‑2262683

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG