Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,330,291	C→G	100%	intergenic (+266/+46)	RCAP_rcc02161 → / ← mrcA	hypothetical protein/penicillin‑binding protein 1A

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,330,291	0	C→G	100.0%	49.7	31	intergenic (+266/+46)	RCAP_rcc02161/mrcA	hypothetical protein/penicillin‑binding protein 1A
Reads supporting (aligned to +/- strand):  new base (20/9):  ref base (0/2):  total (20/11)

CGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCCGGGG....GCCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATCCGAAGGTC  >  NC_014034/2330193‑2330373                                                                                                   |                                                                                       cGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccggg                                                                                      <  1:111410/101‑1 (MQ=255)   tATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccggggg                                                                                    <  1:961024/101‑1 (MQ=255)   tATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCAACTGGGGCCAAAGAtaccgggggtccggggg                                                                                    >  2:1762967/1‑101 (MQ=255)    aTTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggggc                                                                                   >  2:2498458/1‑100 (MQ=255)     tttGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggggcg                                                                                  >  1:1218570/1‑99 (MQ=255)      ttGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggggcgg                                                                                 <  2:495437/101‑4 (MQ=255)       tGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggggcgga                                                                                <  2:3828694/101‑5 (MQ=255)        gcgcCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggggcggag                                                                               <  2:4159660/101‑6 (MQ=255)        gcgcCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggggcggag                                                                               <  1:2498621/101‑6 (MQ=255)        gcgcCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggggcggag                                                                               <  1:4234172/101‑6 (MQ=255)            cAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGGCCAAGAtcccgggggtccgggggcggagcccc                                                                           >  2:64940/1‑92 (MQ=38)            cAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGT.CCGGGG....Gcggagcccc                                                                      <  1:3998271/101‑9 (MQ=38)             aaGAAAAGGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggggcggagccccc                                                                          >  1:282233/1‑91 (MQ=37)             aaGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAtcccgggggtccgggggcggagccccc                                                                          <  2:1780537/101‑11 (MQ=38)             aaGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGT.CCGGGG....Gcggagccccc                                                                     <  2:903932/101‑10 (MQ=38)                               gcATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGccccgggcc                                                        >  1:2942257/1‑94 (MQ=18)                                 aTGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGGCGAgggggcg                                                      >  2:3969899/1‑96 (MQ=21)                                      gggCCCCAGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGCCGAGGCATCGccccc                                                 >  2:2137666/1‑98 (MQ=21)                                              gATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGGGCCGAGGGATCGCCCCGGCGaaaac                                         >  2:2640028/1‑99 (MQ=18)                                                 cACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGCCGGGGCGTGGCCCCCGCGCAAACCCt                                      >  2:2202601/1‑101 (MQ=12)                                                  cccGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCtt                                     <  2:191303/100‑1 (MQ=21)                                                   ccGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGAGCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTa                                    <  2:2243913/101‑1 (MQ=25)                                                           ccGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGAc                            <  2:689728/101‑1 (MQ=25)                                                           ccGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGAc                            <  2:2743233/101‑1 (MQ=25)                                                           ccGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGGGCCGGGGCCTCGCCCCCGCGAAAACCCCTAGTAAACAc                            >  2:3241828/1‑101 (MQ=12)                                                             gggCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGAccg                          <  1:1435186/101‑1 (MQ=25)                                                               gcggcTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGCCGCGGGGGCGCCTCCGCGGAAACCCCTTGTAAAGaacccc                        >  1:3392350/1‑96 (MQ=2)                                                               gcggcTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGCCGAGGCAGCGCCCCGGCGAAATCCCCTAGTAAAAAacgcc                        >  1:4179500/1‑101 (MQ=16)                                                                cggcTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCGAGGCATCTCCTCGGCGAAATCCCTTAGTAAAGAccgccc                       <  1:3960877/101‑1 (MQ=21)                                                                 ggcTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGGGCCCGGGCATCGCCCCCGCGAAAAACCCTCGTAAAAAacgcccc                      >  1:3042813/1‑100 (MQ=2)                                                                   cTTTCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCGGGGCATCGCCTCGGCGAAAACCCCTTGTAAAAACCGCCcccg                    >  2:4078441/1‑101 (MQ=12)                                                                    tttCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGGCCCGGCGCCCCCTCGGCCGAATCCCCTAATGAAAAacgcccgccc                   >  2:3883915/1‑99 (MQ=2)                                                                    tttCACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGCCGAGGCATCGCCTCCGCGAAAAACCTCTGTAAAAAAAGCCCGCgg                   >  2:3823067/1‑101 (MQ=9)                                                                      tccccgggggCCAGAGATCCCGGGGGGCGGGGGGCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGACCGCCCGCGGAc                 <  2:2942036/95‑1 (MQ=18)                                                                       cACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGGGGAGCCCCGGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACa                <  2:3254669/101‑1 (MQ=25)                                                                       cACCTGGGGCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGCCGGGGGATCGCCTCGGCGAAAACCCTTTGTTAAAACCGCCCGCGGACa                >  2:3000139/1‑101 (MQ=12)                                                                            ggggCCAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGCCGAGGGATCGCCCCCGCGGAAAACCTTAGTAAAGAACGCCCGCGGACAgggga           >  1:2308054/1‑99 (MQ=9)                                                                                 cAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCGAGGCCTCGCCCCGGCGAAAAACCCTAGGAAAAAACGCCCGCCGGCACGGCTCCgca      >  2:1757986/1‑99 (MQ=2)                                                                                 cAAAGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGCCGAGGGATCGCCCCGGCGAAAACCCTTAGTAAAGACCGCCCGCGGGCAgggggcccca      >  2:3484652/1‑94 (MQ=12)                                                                                  aaaGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATCCGAAg     <  1:1485819/101‑1 (MQ=21)                                                                                   aaGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGCCGGGGGATCGCCCCCGCGGAAAACCCTAGTAAAAAACGCCCCCGGGCAAGGGGCCGCAgg    >  1:3456122/1‑101 (MQ=0)                                                                                   aaGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGCCGAGGGGTCGCCCCGGCGAAATACCCTTGTAAAAACCGCCCGCGGACAGGGGACCGAAgg    >  2:86705/1‑101 (MQ=9)                                                                                    aGATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGTGCCGAGGCCTCGCCTCCGCGAAAACCCCTCGTAAAGAACCCCCCCGGGCAGGGAGCCGaaagg   >  1:2033442/1‑98 (MQ=2)                                                                                     gATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGCCGAGGGATCGCCCCGGCGAAAACCCCTAGTAAAAAACCCCCCCGGACAGGGATCCGAAgggc  >  1:662947/1‑99 (MQ=9)                                                                                                   |                                                                                       CGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGCCCCGGATCACCGGCGGCCCGGGCGGCTTTCACCTGGGGCCAAAGATCCCGGGGGTCCCGGGG....GCCCCCGGCGGGTCGCCGAGGCATCGCCTCGGCGAAATCCCTTAGTAAAGACCGCCCGCGGACAGGGATCCGAAGGTC  >  NC_014034/2330193‑2330373 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG