Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,467,061 G→C 100% V29L (GTC→CTC)  RCAP_rcc02292 → signal transduction histidine kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,467,0610G→C100.0% 536.4 146V29L (GTC→CTC) RCAP_rcc02292signal transduction histidine kinase
Reads supporting (aligned to +/- strand):  new base (75/71):  ref base (0/0):  total (75/71)

Only 100 of 161 total aligned reads displayed.

GGGCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGGTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGT  >  NC_014034/2466963‑2467158
                                                                                                  |                                                                                                  
   ccGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGc                                                                                               >  2:1209740/1‑101 (MQ=255)
     gCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTa                                                                                             <  2:1347217/101‑1 (MQ=255)
     gCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTa                                                                                             <  2:1227867/101‑1 (MQ=255)
        ggcggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgc                                                                                          >  2:111516/1‑101 (MQ=255)
        ggcggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgc                                                                                          >  1:197691/1‑101 (MQ=255)
        ggcggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgc                                                                                          <  1:2161041/101‑1 (MQ=255)
         gcggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgcg                                                                                         <  2:319477/101‑1 (MQ=255)
          cggcATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATgcgc                                                                                        <  2:484212/101‑1 (MQ=255)
             cATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGa                                                                                     <  1:856259/101‑1 (MQ=255)
               tGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGAcc                                                                                   <  2:2803874/101‑1 (MQ=255)
               tGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGAcc                                                                                   >  1:1507977/1‑101 (MQ=255)
               tGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGAcc                                                                                   <  1:285676/101‑1 (MQ=255)
                gATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCGATGCGCAGAccc                                                                                  >  2:3554695/1‑101 (MQ=255)
                 aTCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCt                                                                                 >  2:1720762/1‑101 (MQ=255)
                 aTCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCt                                                                                 <  1:284275/101‑1 (MQ=255)
                  tCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTg                                                                                <  2:1739373/101‑1 (MQ=255)
                  tCCGGGGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTg                                                                                <  1:1024053/101‑1 (MQ=255)
                     ggTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGcc                                                                             >  2:644078/1‑101 (MQ=255)
                      gTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCa                                                                            <  1:2212841/101‑1 (MQ=255)
                      gTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCa                                                                            <  2:2720663/101‑1 (MQ=255)
                       tGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCAc                                                                           <  1:1702221/101‑1 (MQ=255)
                         gCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCg                                                                         <  2:3447291/101‑1 (MQ=255)
                         gCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCg                                                                         <  2:1616836/101‑1 (MQ=255)
                          cGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.a                                                                       <  1:1949272/101‑1 (MQ=255)
                           gACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.aa                                                                      <  1:4084484/101‑1 (MQ=255)
                           gACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.aa                                                                      >  2:661735/1‑101 (MQ=255)
                           gACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.aa                                                                      >  2:65294/1‑101 (MQ=255)
                               cgGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACggg                                                                  >  1:1157872/1‑101 (MQ=255)
                                ggCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGgc                                                                 >  2:4390684/1‑101 (MQ=255)
                                ggCACGGGCGGGCTTGGGGTGCGGGTGACGGGGGTCATGGGGGGGGTGGTGTCCGCGGTCCCCCTGCCCACGCATGCGCAGACCCTGGCCCCCGCAACggg                                                                  >  2:3260476/1‑101 (MQ=255)
                                 gCACGGGCGGTCTGTGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGgcc                                                                <  2:921612/101‑1 (MQ=255)
                                 gCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGgcc                                                                <  1:1567562/101‑1 (MQ=255)
                                 gCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGgcc                                                                >  1:4147248/1‑101 (MQ=255)
                                   aCGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGgccgc                                                              >  1:2550067/1‑101 (MQ=255)
                                    cGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGgccgcc                                                             >  1:336522/1‑101 (MQ=255)
                                    cGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGgccgcc                                                             <  2:3772003/101‑1 (MQ=255)
                                    cGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGgccgcc                                                             <  2:66681/101‑1 (MQ=255)
                                    cGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGgccgcc                                                             >  1:3697420/1‑101 (MQ=255)
                                     gggCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGccgccg                                                            <  1:1236761/101‑1 (MQ=255)
                                     gggCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTAAGCTATGCGCAGACCCTGGCCACCG.AACGGGccgccg                                                            >  1:3562780/1‑101 (MQ=255)
                                        cGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGaaa                                                         <  1:3944621/101‑1 (MQ=255)
                                        cGGTCTGGCGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGaaa                                                         <  1:1148001/101‑1 (MQ=255)
                                          gTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAAc                                                       <  1:2184091/101‑1 (MQ=255)
                                           tCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAAcc                                                      <  2:1321961/101‑1 (MQ=255)
                                           tCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAAcc                                                      >  1:1859127/1‑101 (MQ=255)
                                             tGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGt                                                    <  1:3767214/101‑1 (MQ=255)
                                              ggggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTc                                                   <  1:537275/101‑1 (MQ=255)
                                              ggggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTc                                                   >  1:996202/1‑101 (MQ=255)
                                               gggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCt                                                  <  2:751143/101‑1 (MQ=255)
                                               gggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCt                                                  >  1:2556114/1‑101 (MQ=255)
                                                ggCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTg                                                 <  1:2291444/101‑1 (MQ=255)
                                                 gCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTgc                                                >  1:3611562/1‑101 (MQ=255)
                                                  cTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTgcg                                               >  1:795349/1‑101 (MQ=255)
                                                   tGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTgcgc                                              >  1:2344894/1‑101 (MQ=255)
                                                   tGCGGCTGACGGTGATCATGGGGGCGGGGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTgcgc                                              >  1:631454/1‑101 (MQ=255)
                                                    gCGGCTGACGGTGGTCGTGGGGGCGGTGCTGACGCTGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGATAACCGTCTgagcg                                             >  2:1999265/1‑101 (MQ=255)
                                                    gCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTgcgcg                                             >  1:560264/1‑101 (MQ=255)
                                                     cGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTgcgcgc                                            >  2:161368/1‑101 (MQ=255)
                                                     cGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTgcgcgc                                            >  2:807150/1‑101 (MQ=255)
                                                          gACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGa                                       >  2:3527008/1‑101 (MQ=255)
                                                           aCGGTGATCATGGGGGCGGTGCTGCCGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGAt                                      <  1:4190250/101‑1 (MQ=255)
                                                            cGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCT.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATc                                     <  2:1021531/101‑1 (MQ=255)
                                                              gTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCt                                   <  2:4154067/101‑1 (MQ=255)
                                                              gTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCt                                   <  2:2083954/101‑1 (MQ=255)
                                                              gTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCCCGCGGCGATCCt                                   >  2:368038/1‑101 (MQ=255)
                                                               tGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTg                                  <  1:3481128/101‑1 (MQ=255)
                                                               tGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTg                                  <  2:3597394/101‑1 (MQ=255)
                                                                gATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTgg                                 >  2:2538057/1‑101 (MQ=255)
                                                                gATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTgg                                 >  1:3905023/1‑101 (MQ=255)
                                                                gATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTgg                                 >  1:4110983/1‑101 (MQ=255)
                                                                 aTCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTggg                                <  1:514244/101‑1 (MQ=255)
                                                                 aTCATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTggg                                >  2:1548506/1‑101 (MQ=255)
                                                                   cATGGGGGCGTTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCg                              <  1:3071442/101‑1 (MQ=255)
                                                                   cATGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCg                              >  1:920771/1‑101 (MQ=255)
                                                                    aggggggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCga                             <  1:1058544/99‑1 (MQ=255)
                                                                     tGGGGGCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCgag                            <  2:860525/101‑1 (MQ=255)
                                                                       ggggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGAc                          >  1:3316552/1‑101 (MQ=255)
                                                                        gggCGGTGCTGACGCCGCTCTCCGTGCTCAGCTATGCGCAGTCCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCgagaag                         <  2:2141226/101‑3 (MQ=255)
                                                                         ggCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGc                        >  2:2426427/1‑101 (MQ=255)
                                                                          gCGGTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCt                       <  1:644146/101‑1 (MQ=255)
                                                                            ggTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCTCCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGa                     >  2:3693519/1‑101 (MQ=255)
                                                                             gTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGAt                    >  1:3542962/1‑101 (MQ=255)
                                                                             gTGCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGAt                    <  1:171333/101‑1 (MQ=255)
                                                                               gCTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATgg                  >  1:4031678/1‑101 (MQ=255)
                                                                               gCTGACGCCGCTCGCCATGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATgg                  <  2:3562532/101‑1 (MQ=255)
                                                                                cTGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgc                 <  2:4024838/101‑1 (MQ=255)
                                                                                 tGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgcc                >  1:2824490/1‑101 (MQ=255)
                                                                                 tGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgcc                <  1:3322735/101‑1 (MQ=255)
                                                                                 tGACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgcc                >  2:1924576/1‑101 (MQ=255)
                                                                                  gACGCCGCTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgccg               >  2:3826463/1‑101 (MQ=255)
                                                                                    cgccgcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGgccgcc             >  2:2510453/1‑101 (MQ=255)
                                                                                     gccgcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGccgccg            <  2:197664/101‑1 (MQ=255)
                                                                                       cgcTCGCCGTGCTCAGCTATGCGCAGCCCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgcg          <  2:3967547/101‑1 (MQ=255)
                                                                                       cgcTCGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgcg          <  2:336487/101‑1 (MQ=255)
                                                                                       cccTCGCCGTGCTCAGCTAGGCGCAGCCCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCcgccgcg          <  2:2910078/99‑1 (MQ=255)
                                                                                           cGCCGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCgccgcgccgc      >  2:1882875/1‑101 (MQ=255)
                                                                                              cGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAgg   >  2:3977384/1‑101 (MQ=255)
                                                                                              cGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAgg   >  1:3363845/1‑101 (MQ=255)
                                                                                              cGTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAgg   <  2:2344744/101‑1 (MQ=255)
                                                                                               gTGCTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGt  >  2:2247852/1‑101 (MQ=255)
                                                                                                  |                                                                                                  
GGGCCGCAGGCGGCATGATCCGGTGGCGACGCGGCACGGGCGGTCTGGGGCTGCGGCTGACGGTGATCATGGGGGCGGTGCTGACGCCGCTCGCCGTGGTCAGCTATGCGCAGACCCTGGCCACCG.AACGGGCCGCCGAAAACCGTCTGCGCGCGGCGATCCTGGGCGAGACGCTGATGGCCGCCGCGCCGCAGGT  >  NC_014034/2466963‑2467158

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG