Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,016 C→G 6.6% intergenic (+64/‑178) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0160C→G 6.6% 19.4 0.0 ‑0.0166intergenic (+64/‑178)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/7):  ref base (57/98):  total (61/105)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.86e-01

Only 100 of 187 total aligned reads displayed.

CGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.A.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGA.AGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC  >  NC_014034/2493917‑2494115
                                                                                                     |                                                                                                           
cgccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGGTATTTGCAGCCAAATTAAAcc                                                                                                             >  1:4142835/1‑101 (MQ=18)
 gccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGCTATTTGCAGCAAGATGAAAACg                                                                                                            >  2:151467/1‑101 (MQ=18)
 gccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                                                                                            <  2:236091/101‑1 (MQ=21)
 gccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCg                                                                                                            >  1:1831324/1‑101 (MQ=21)
    gcggcgGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                                                                         <  1:2455584/101‑1 (MQ=21)
    gcggcgGGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                                                                         <  2:112598/101‑1 (MQ=21)
     cggcggGCGCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATg                                                                                                        <  1:2893087/101‑1 (MQ=21)
      ggcgggcgCGCGGAACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGc                                                                                                       >  2:1723956/1‑101 (MQ=18)
         gggcgCGCGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                    >  2:628191/1‑101 (MQ=21)
           gcgcgcGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                  <  1:2083954/101‑1 (MQ=21)
           gcgcgcGGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                  <  1:1816798/101‑1 (MQ=21)
            ggcgcgGCGCGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                 <  2:3910641/100‑1 (MQ=18)
            cgcgcgGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                 <  1:3510733/101‑1 (MQ=21)
            cgcgcgGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                 <  1:2563115/101‑1 (MQ=21)
              cgcgGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                               <  2:420997/101‑1 (MQ=21)
               gcgGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                              <  1:736814/101‑1 (MQ=21)
               gcgGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                              <  1:602055/101‑1 (MQ=21)
                cgGCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                             <  1:1235454/101‑1 (MQ=21)
                 ggCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                            <  2:1014675/101‑1 (MQ=21)
                 ggCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                            <  2:2326949/101‑1 (MQ=21)
                  gCACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGc                                                                                           >  2:2713331/1‑101 (MQ=21)
                   cACGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                                          >  2:3568212/1‑101 (MQ=21)
                    aCGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                         >  2:1249258/1‑101 (MQ=21)
                    aCGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                         <  1:1075202/101‑1 (MQ=21)
                     gggcAGGCCGAGTA.AGGGGGGGGCGCGGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                        <  2:2607488/98‑1 (MQ=18)
                     cGACGGGCCGAGTAGG.GGGGGGGCGCTCCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATAAAACCGGATGCCTTTCATCTTGCCCg                                                                                        <  2:2023637/101‑1 (MQ=18)
                     cGACAGGCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTATCATCTTGCCCg                                                                                        <  1:2350473/101‑1 (MQ=18)
                        cAGGCCGATTAGG.GGGGGGGCGCTGCCCCCCGTCCGCAATCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                     <  2:1865859/101‑1 (MQ=18)
                         aGGCCCAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCACAAAat                                                                                    <  2:137179/101‑1 (MQ=18)
                          ggCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                   >  2:4043230/1‑101 (MQ=21)
                          ggCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                   <  1:1698919/101‑1 (MQ=21)
                           gCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                  <  1:2503497/101‑1 (MQ=21)
                           gCCGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                  <  1:1991105/101‑1 (MQ=21)
                           atctaGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                  <  1:4384874/97‑1 (MQ=18)
                            ccGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                 <  2:183852/101‑1 (MQ=18)
                            ccGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                 <  1:1463652/101‑1 (MQ=21)
                             cgggtaagggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                 <  1:3026218/92‑1 (MQ=18)
                             cGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                <  1:3699225/101‑1 (MQ=18)
                             cGAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                <  2:1380647/101‑1 (MQ=18)
                              gAGTA.AGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                               <  1:3869050/101‑1 (MQ=18)
                                      gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                        <  1:701182/101‑1 (MQ=255)
                                        gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                      <  2:2241220/101‑1 (MQ=255)
                                         ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                     <  1:2149874/101‑1 (MQ=255)
                                             cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                <  1:2374334/101‑1 (MQ=255)
                                              gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                             <  1:3097965/101‑1 (MQ=255)
                                               cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                           <  2:1195484/101‑1 (MQ=255)
                                                 gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTTCCCGAAATATCTCTGTGGGGTAA.TGGT..G.atc                                                         >  1:1313118/1‑99 (MQ=18)
                                                 gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                         <  1:1081080/101‑3 (MQ=255)
                                                  cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                        <  2:3332561/101‑4 (MQ=255)
                                                  cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                        <  1:2808866/101‑4 (MQ=255)
                                                   ccccG.TCCTCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATTCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                      <  1:1331605/101‑6 (MQ=18)
                                                   ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                      >  1:1562174/1‑96 (MQ=255)
                                                     cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                     >  1:3570867/5‑95 (MQ=255)
                                                     cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                     <  2:1226321/97‑7 (MQ=255)
                                                       cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                   >  1:3401873/3‑93 (MQ=255)
                                                       cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                   <  1:573662/99‑9 (MQ=255)
                                                         tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagaggg                                                 >  2:2511595/1‑91 (MQ=255)
                                                         tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccagaggg                                                 >  2:3007661/1‑91 (MQ=18)
                                                              aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCa                                          >  2:2302328/1‑101 (MQ=18)
                                                              aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGCa                                          >  1:4364755/1‑101 (MQ=18)
                                                               aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCag                                         <  2:413502/101‑2 (MQ=18)
                                                               aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCag                                         <  1:2028967/101‑2 (MQ=18)
                                                                gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCagc                                        >  2:450441/1‑99 (MQ=18)
                                                                  ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGCagcgc                                      >  1:815323/1‑97 (MQ=17)
                                                                     cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cccc                                 >  2:688702/1‑101 (MQ=17)
                                                                      tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG..AGGGGGGC.AGCG.ccccc                                <  2:4142574/101‑1 (MQ=17)
                                                                           ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cccccctgcc                           <  2:2951645/101‑6 (MQ=17)
                                                                             gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CCCCCTGcccc                          >  1:2784255/1‑101 (MQ=0)
                                                                             gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CCCCCTGcccc                          <  1:2186554/101‑1 (MQ=0)
                                                                             gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGcccc                          >  2:1325244/1‑101 (MQ=18)
                                                                              ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcg                         >  2:1447132/1‑101 (MQ=0)
                                                                              ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.GGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCcg                         >  1:4009471/1‑101 (MQ=17)
                                                                              ggATANNTGCAGAAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCcg                         <  1:1594892/101‑1 (MQ=17)
                                                                               gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCcgg                        >  1:2613171/1‑101 (MQ=17)
                                                                                gtatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgcc                       <  2:1429237/100‑3 (MQ=2)
                                                                                atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgcc                       <  1:1155047/101‑3 (MQ=2)
                                                                                atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgcc                       <  2:2819530/101‑3 (MQ=2)
                                                                                 tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCcggcg                      >  2:3118786/1‑101 (MQ=18)
                                                                                 tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.A.GCCA.GAGGGGGC.AGCGCCCCCCTGCCCcggcgg                     >  2:225289/1‑101 (MQ=17)
                                                                                 tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCACcggcg                      >  2:341801/1‑101 (MQ=17)
                                                                                  atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgccgg                     <  2:337533/101‑1 (MQ=2)
                                                                                  atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCcggcgg                     >  1:947985/1‑101 (MQ=17)
                                                                                  atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.A.GACAGAGGGGGGC.AGCGCCCCCCTGCCCcggcgg                     >  1:477110/1‑101 (MQ=18)
                                                                                  anNTGCNGCAAGATGAANGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgccgg                     <  2:1707762/101‑1 (MQ=2)
                                                                                    ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgccggat                   >  2:91130/1‑99 (MQ=2)
                                                                                    ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTc                   <  2:3706895/101‑1 (MQ=17)
                                                                                       cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCCGCGGGCCgg                >  2:3847046/1‑100 (MQ=14)
                                                                                        agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg               >  2:1075039/1‑101 (MQ=18)
                                                                                        agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg               >  1:3046297/1‑101 (MQ=17)
                                                                                         gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTgg              >  1:1966347/1‑101 (MQ=17)
                                                                                          caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggc             <  1:3185265/101‑1 (MQ=17)
                                                                                           aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcg            <  2:1110206/101‑1 (MQ=17)
                                                                                               tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGgcgc        >  2:4269411/1‑101 (MQ=17)
                                                                                                 aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCt      <  1:4261496/101‑1 (MQ=17)
                                                                                                   aCCGGATGCCTTTCATCTTGCCCGAAATATCCTGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg    >  2:3138641/1‑101 (MQ=18)
                                                                                                   aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg    <  1:2335769/101‑1 (MQ=17)
                                                                                                     cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGAGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc  >  2:3217971/1‑101 (MQ=18)
                                                                                                     cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.ACG.CCAG.AGGGGGGC.CGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc  >  2:2470170/1‑101 (MQ=18)
                                                                                                     cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGGGGCGGCGCCTTGAc  >  1:2467268/1‑101 (MQ=17)
                                                                                                     cGGATGCCTTTCATCTTGCCCGAAATACCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc  <  1:1762593/101‑1 (MQ=17)
                                                                                                     |                                                                                                           
CGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.A.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGA.AGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAC  >  NC_014034/2493917‑2494115

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG