Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,018 G→C 6.5% intergenic (+66/‑176) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0180G→C 6.5% 20.6 0.0 ‑0.1168intergenic (+66/‑176)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/7):  ref base (61/96):  total (65/103)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.63e-01

Only 100 of 181 total aligned reads displayed.

CGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493920‑2494117
                                                                                                    |                                                                                                           
 gcggcgGGCGCGCGGGACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                                                                           <  2:2440153/101‑1 (MQ=18)
 gcggcgGGCGCGCGGCACGACAGGCCGAGTAAA.GGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGag                                                                                                           >  2:1337476/1‑100 (MQ=18)
 gcggcgGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                                                                           <  2:112598/101‑1 (MQ=21)
 gcggcgGGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                                                                           <  1:2455584/101‑1 (MQ=21)
  cggcggGCGCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATg                                                                                                          <  1:2893087/101‑1 (MQ=21)
    gcgggcgCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGcc                                                                                                        <  1:1811761/101‑1 (MQ=21)
      gggcgCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                      >  2:4365982/1‑101 (MQ=21)
      gggcgCGCGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                      >  2:628191/1‑101 (MQ=21)
        gcgcgcGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                    <  1:2083954/101‑1 (MQ=21)
         ggcgcgGCGCGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                   <  2:3910641/100‑1 (MQ=18)
         cgcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                   <  2:171363/101‑1 (MQ=21)
         cgcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                   <  1:3510733/101‑1 (MQ=21)
         cgcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                   <  2:1105378/101‑1 (MQ=21)
           cgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                                 >  1:1322135/1‑101 (MQ=21)
           cgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                                 <  2:420997/101‑1 (MQ=21)
            gcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                                <  1:602055/101‑1 (MQ=21)
            gcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                                <  1:736814/101‑1 (MQ=21)
              ggCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                              <  2:2326949/101‑1 (MQ=21)
              ggCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                              <  2:1014675/101‑1 (MQ=21)
              ggCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                              <  2:4069210/101‑1 (MQ=21)
                cACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                                                                            >  2:3568212/1‑101 (MQ=21)
                 aCGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                           <  1:1075202/101‑1 (MQ=21)
                 aCGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                           >  2:1249258/1‑101 (MQ=21)
                 aCGACAGGCCGAGT.AAGGGGGGGGCGCGGCCCCCCGCCCGCAAGCCGAACCCCCCCAGGATATTTGCAGCAAAACGAAAACGGAGACCTTTCAACTTGccc                                                                                           >  1:1666772/1‑101 (MQ=255)
                  gggcAGGCCGAGT.AAGGGGGGGGCGCGGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                          <  2:2607488/98‑1 (MQ=18)
                  cGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                          <  1:4347640/101‑1 (MQ=21)
                  cGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                          <  1:2561155/101‑1 (MQ=21)
                  cGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                          <  2:2992867/101‑1 (MQ=21)
                   gACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGa                                                                                         >  1:2348065/1‑101 (MQ=21)
                      aGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCGAAATGAAACCGGGTGCCTTTCATCTTGCCCGAAat                                                                                      >  1:3626024/1‑101 (MQ=18)
                       ggCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                     <  1:269917/101‑1 (MQ=21)
                       ggCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                     <  2:1477498/101‑1 (MQ=21)
                        gCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                    <  1:1991105/101‑1 (MQ=21)
                        atctaGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                    <  1:4384874/97‑1 (MQ=18)
                         ccGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                   <  1:1463652/101‑1 (MQ=21)
                          cgggtaagggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                   <  1:3026218/92‑1 (MQ=18)
                               taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                              <  1:72196/97‑1 (MQ=255)
                                    ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                         <  2:1735060/101‑1 (MQ=255)
                                    ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                         <  2:2757045/101‑1 (MQ=255)
                                     gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                        <  2:2241220/101‑1 (MQ=255)
                                      ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                       <  1:2149874/101‑1 (MQ=255)
                                       gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                     >  1:1378452/1‑101 (MQ=255)
                                           gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                               <  1:3097965/101‑1 (MQ=255)
                                            cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                             <  2:1195484/101‑1 (MQ=255)
                                             tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                            <  2:2495794/101‑2 (MQ=255)
                                              gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                           <  1:1081080/101‑3 (MQ=255)
                                               cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                          <  2:3332561/101‑4 (MQ=255)
                                               cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                          <  1:2808866/101‑4 (MQ=255)
                                                ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                        >  1:1562174/1‑96 (MQ=255)
                                                  cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                       <  2:1226321/97‑7 (MQ=255)
                                                  cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                       >  1:3570867/5‑95 (MQ=255)
                                                    cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                     <  1:573662/99‑9 (MQ=255)
                                                    cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                     >  1:3401873/3‑93 (MQ=255)
                                                     gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccagagg                                                    >  1:1827773/2‑92 (MQ=21)
                                                      tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagaggg                                                   >  2:2511595/1‑91 (MQ=255)
                                                      tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagaggg                                                   <  1:1724067/101‑11 (MQ=255)
                                                      tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccagaggg                                                   >  2:3007661/1‑91 (MQ=18)
                                                           aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCa                                             >  2:2302328/1‑101 (MQ=18)
                                                            aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCag                                            <  2:4028881/101‑2 (MQ=18)
                                                            aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCag                                            <  1:2028967/101‑2 (MQ=18)
                                                               ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGCagcgc                                         >  1:815323/1‑97 (MQ=17)
                                                                  cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cc.cc                                   >  2:688702/1‑101 (MQ=17)
                                                                   tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cc.ccc                                  >  1:2395499/1‑101 (MQ=17)
                                                                   tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCG.cc.ccc                                  <  2:4142574/101‑1 (MQ=17)
                                                                    ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cc.cccc                                 <  2:1952332/101‑2 (MQ=17)
                                                                    ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cc.cccc                                 >  1:2365263/1‑100 (MQ=17)
                                                                      ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cc.cccctg                               <  1:4366253/101‑4 (MQ=17)
                                                                      ccccGGGATATGTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cc.cccctg                               <  2:1322038/101‑4 (MQ=17)
                                                                         cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATACCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGccc                             >  1:2563268/1‑101 (MQ=17)
                                                                          gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CC.CCCTGcccc                            <  1:2186554/101‑1 (MQ=0)
                                                                          gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CC.CCCTGcccc                            >  1:2784255/1‑101 (MQ=0)
                                                                           ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.GGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCcg                           >  1:4009471/1‑101 (MQ=17)
                                                                             gtatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgcc                         <  2:1429237/100‑3 (MQ=2)
                                                                             atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgcc                         <  1:1155047/101‑3 (MQ=2)
                                                                              tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCcggcg                        >  2:3118786/1‑101 (MQ=18)
                                                                              tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GCCA.GAGGGGGC.AGCGCCC.CCCTGCCCcggcgg                       >  2:225289/1‑101 (MQ=17)
                                                                              tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCACcggcg                        >  2:341801/1‑101 (MQ=17)
                                                                               atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCcggcgg                       >  1:947985/1‑101 (MQ=17)
                                                                               atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GACAGAGGGGGGC.AGCGCCC.CCCTGCCCcggcgg                       >  1:477110/1‑101 (MQ=18)
                                                                               anNTGCNGCAAGATGAANGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgccgg                       <  2:1707762/101‑1 (MQ=2)
                                                                                 ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgccggat                     >  2:91130/1‑99 (MQ=2)
                                                                                 ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTc                     <  2:3706895/101‑1 (MQ=17)
                                                                                  tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.CCGCCCTGCCCCGGCGGTcc                    >  2:1376656/1‑101 (MQ=18)
                                                                                   gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCg                   >  2:3410585/1‑101 (MQ=18)
                                                                                    cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCCGCGGGCCgg                  >  2:3847046/1‑100 (MQ=14)
                                                                                     agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTg                 >  2:1075039/1‑101 (MQ=18)
                                                                                      gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTgg                >  1:1966347/1‑101 (MQ=17)
                                                                                       caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc               >  1:3860326/1‑101 (MQ=18)
                                                                                       caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc               <  1:3185265/101‑1 (MQ=17)
                                                                                       caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc               >  2:503747/1‑101 (MQ=17)
                                                                                        aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcg              >  2:2922315/1‑101 (MQ=18)
                                                                                            tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGgcgc          >  2:4269411/1‑101 (MQ=17)
                                                                                            tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.CCTCCCTGCCCCGGCGGTCCGTGGCGgggc          >  1:414463/1‑101 (MQ=17)
                                                                                               aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt       <  1:1249351/101‑1 (MQ=17)
                                                                                                aCCGGATGCCTTTCATCTTGCCCGAAATATCCTGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg      >  2:3138641/1‑101 (MQ=18)
                                                                                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc    >  2:1859906/1‑101 (MQ=18)
                                                                                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGAGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc    >  2:3217971/1‑101 (MQ=18)
                                                                                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGGGGCGGCGCCTTGAc    >  1:2467268/1‑101 (MQ=17)
                                                                                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAG.AGGGGGGC.CGCGCCC.CCCTGCCCCGGCGGCCCGGGGCGGGGCCTTgcc    >  1:3069832/1‑99 (MQ=12)
                                                                                                  cGGATGCCTTTCATCTTGCCCGAAATACCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc    <  1:1762593/101‑1 (MQ=17)
                                                                                                    |                                                                                                           
CGCGGCGGGCGCGCGGCACGACAGGCCGAGT.AA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGG  >  NC_014034/2493920‑2494117

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG