Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,023 C→A 6.6% intergenic (+71/‑171) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0230C→A 6.6% 16.9 0.0 ‑0.0165intergenic (+71/‑171)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/7):  ref base (62/92):  total (66/99)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 177 total aligned reads displayed.

GCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGG  >  NC_014034/2493924‑2494121
                                                                                                    |                                                                                                          
gcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGcc                                                                                                            <  1:1811761/101‑1 (MQ=21)
 cgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                                           <  2:1168021/101‑1 (MQ=21)
  gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                          >  2:628191/1‑101 (MQ=21)
  gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                          <  1:4074397/101‑1 (MQ=21)
    gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                        <  1:2083954/101‑1 (MQ=21)
    gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                        <  1:4427462/101‑1 (MQ=21)
     ggcgcgGCGCGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                       <  2:3910641/100‑1 (MQ=18)
     cgcgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                       <  2:171363/101‑1 (MQ=21)
     cgcgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                       <  1:2563115/101‑1 (MQ=21)
     cgcgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                       <  2:1105378/101‑1 (MQ=21)
       cgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                                     <  2:420997/101‑1 (MQ=21)
       cgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                                     >  1:1322135/1‑101 (MQ=21)
        gcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                                    <  1:736814/101‑1 (MQ=21)
        gcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                                    <  1:602055/101‑1 (MQ=21)
         cgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                                   <  2:3551263/101‑1 (MQ=21)
          ggCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                                  <  2:1014675/101‑1 (MQ=21)
          ggCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                                  <  2:2326949/101‑1 (MQ=21)
             aCGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                               >  2:1249258/1‑101 (MQ=21)
             aCGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                               <  1:1075202/101‑1 (MQ=21)
             aCGACAGGCCGAGTAAGGGGGGGGCGCGGCCCCCCGCCCGCAAGCCGAACCCCCCCAGGATATTTGCAGCAAAACGAAAACGGAGACCTTTCAACTTGccc                                                                                               >  1:1666772/1‑101 (MQ=255)
              gggcAGGCCGAGTAAGGGGGGGGCGCGGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                              <  2:2607488/98‑1 (MQ=18)
              cGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                              <  2:2992867/101‑1 (MQ=21)
              cGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTATCATCTTGCCCg                                                                                              <  1:2350473/101‑1 (MQ=18)
                aCAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                                                                                            <  2:3266273/101‑1 (MQ=21)
                  aGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCGAAATGAAACCGGGTGCCTTTCATCTTGCCCGAAat                                                                                          >  1:3626024/1‑101 (MQ=18)
                   ggCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                         >  2:4043230/1‑101 (MQ=21)
                   ggCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                         <  1:269917/101‑1 (MQ=21)
                   ggCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                         <  1:1698919/101‑1 (MQ=21)
                   ggCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                         >  1:2020565/1‑101 (MQ=21)
                    atctaGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                        <  1:4384874/97‑1 (MQ=18)
                     cgggtaagggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                       <  1:3026218/92‑1 (MQ=18)
                     ccGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                       <  2:183852/101‑1 (MQ=18)
                     ccGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                       <  1:1463652/101‑1 (MQ=21)
                      cGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                      <  2:1380647/101‑1 (MQ=18)
                            aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                                >  1:281857/3‑101 (MQ=255)
                              gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                              <  1:701182/101‑1 (MQ=255)
                               ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                             <  2:2757045/101‑1 (MQ=255)
                                gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                            <  2:2241220/101‑1 (MQ=255)
                                 ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                           <  1:2149874/101‑1 (MQ=255)
                                     cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                      <  1:2374334/101‑1 (MQ=255)
                                     cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                      <  2:1030138/101‑1 (MQ=255)
                                      gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                   <  1:3097965/101‑1 (MQ=255)
                                        tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                <  2:2495794/101‑2 (MQ=255)
                                        tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                <  1:3568460/101‑2 (MQ=255)
                                         gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                               <  1:1081080/101‑3 (MQ=255)
                                          cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                              <  2:3332561/101‑4 (MQ=255)
                                           ccccG.TCCTCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATTCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                            <  1:1331605/101‑6 (MQ=18)
                                           ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                            >  1:1562174/1‑96 (MQ=255)
                                             cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                           <  1:3438156/97‑7 (MQ=255)
                                             cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                           <  2:1226321/97‑7 (MQ=255)
                                               cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                         >  1:3401873/3‑93 (MQ=255)
                                               cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                         <  1:573662/99‑9 (MQ=255)
                                                gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccagagg                                                        >  1:1827773/2‑92 (MQ=21)
                                                 tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagaggg                                                       >  2:2511595/1‑91 (MQ=255)
                                                 tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccagaggg                                                       >  2:3007661/1‑91 (MQ=18)
                                                      aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCa                                                 >  2:2302328/1‑101 (MQ=18)
                                                      aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGCa                                                 >  1:4364755/1‑101 (MQ=18)
                                                       aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCag                                                <  2:413502/101‑2 (MQ=18)
                                                        gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCagc                                               >  2:450441/1‑99 (MQ=18)
                                                          ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGCagcgc                                             >  1:815323/1‑97 (MQ=17)
                                                             cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cc.cc                                       >  2:688702/1‑101 (MQ=17)
                                                              tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cc.ccc                                      >  1:2395499/1‑101 (MQ=17)
                                                              tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCG.cc.ccc                                      <  2:4142574/101‑1 (MQ=17)
                                                               ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cc.cccc                                     <  2:1952332/101‑2 (MQ=17)
                                                                cccccGGGATATTTGCAGCAAGATGAAACCGGATCCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.cc.cccct                                    <  1:19700/101‑3 (MQ=17)
                                                                    cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGACC.CCCTGccc                                 >  2:1214419/1‑101 (MQ=17)
                                                                     gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGcccc                                >  2:1325244/1‑101 (MQ=18)
                                                                      ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcg                               >  2:1447132/1‑101 (MQ=0)
                                                                      ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.GGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCcg                               >  1:4009471/1‑101 (MQ=17)
                                                                       gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgc                              <  1:2793530/101‑2 (MQ=0)
                                                                       gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgc                              >  2:1683206/1‑100 (MQ=2)
                                                                       gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCcgg                              >  1:2613171/1‑101 (MQ=17)
                                                                        atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgcc                             <  1:1155047/101‑3 (MQ=2)
                                                                         tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCcggcg                            >  2:3118786/1‑101 (MQ=18)
                                                                          atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCcggcgg                           >  1:947985/1‑101 (MQ=17)
                                                                          atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GACAGAGGGGGGC.AGCGCCC.CCCTGCCCcggcgg                           >  1:477110/1‑101 (MQ=18)
                                                                            ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgccggat                         >  2:91130/1‑99 (MQ=2)
                                                                            ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTc                         <  2:3706895/101‑1 (MQ=17)
                                                                             tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.CCGCCCTGCCCCGGCGGTcc                        >  2:1376656/1‑101 (MQ=18)
                                                                              gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCg                       >  2:3410585/1‑101 (MQ=18)
                                                                               cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCCGCGGGCCgg                      >  2:3847046/1‑100 (MQ=14)
                                                                                agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTg                     >  1:3046297/1‑101 (MQ=17)
                                                                                 gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTgg                    >  1:1966347/1‑101 (MQ=17)
                                                                                  caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                   >  1:3860326/1‑101 (MQ=18)
                                                                                  caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                   <  1:3185265/101‑1 (MQ=17)
                                                                                  caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                   >  2:503747/1‑101 (MQ=17)
                                                                                   aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcg                  >  2:2922315/1‑101 (MQ=18)
                                                                                     gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAA.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcggc                <  1:2171140/101‑1 (MQ=18)
                                                                                       tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGgcgc              <  2:225445/101‑1 (MQ=17)
                                                                                       tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGgcgc              >  2:4269411/1‑101 (MQ=17)
                                                                                         aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCt            <  1:4261496/101‑1 (MQ=17)
                                                                                          aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt           <  1:1249351/101‑1 (MQ=17)
                                                                                           aCCGGATGCCTTTCATCTTGCCCGAAATATCCTGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg          >  2:3138641/1‑101 (MQ=18)
                                                                                           aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg          <  1:2335769/101‑1 (MQ=17)
                                                                                             cGGATTCCTTTCATCTTGCCGGAAATACCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc        <  2:3625771/101‑1 (MQ=14)
                                                                                             cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc        >  2:1859906/1‑101 (MQ=18)
                                                                                             cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGGGGCGGCGCCTTGAc        >  1:2467268/1‑101 (MQ=17)
                                                                                               gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGGGCCTTGAcgg      >  1:1750029/1‑101 (MQ=17)
                                                                                                 tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCACCGC.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc    <  1:2541303/101‑1 (MQ=18)
                                                                                                   ccTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg  <  2:2365113/101‑1 (MQ=17)
                                                                                                    |                                                                                                          
GCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGG  >  NC_014034/2493924‑2494121

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG