Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,531,713 G→C 100% D496E (GAC→GAG RCAP_rcc02362 ← competence protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,531,7130G→C100.0% 605.6 163D496E (GAC→GAGRCAP_rcc02362competence protein
Reads supporting (aligned to +/- strand):  new base (102/61):  ref base (0/0):  total (102/61)

Only 100 of 177 total aligned reads displayed.

GACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAAGTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCGG  >  NC_014034/2531615‑2531810
                                                                                                  |                                                                                                 
gACAGCCCCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTc                                                                                                 <  2:1223378/101‑1 (MQ=255)
gACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTc                                                                                                 >  1:2805911/1‑101 (MQ=255)
 aCAGCCCCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcg                                                                                                <  1:3832782/101‑1 (MQ=255)
 aCAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcg                                                                                                >  1:2438150/1‑101 (MQ=255)
   aGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggc                                                                                              >  1:2176141/1‑101 (MQ=255)
    gccccgccgCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggcc                                                                                             <  1:411801/98‑1 (MQ=255)
         gccgccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggccacggc                                                                                        <  1:3556316/101‑1 (MQ=255)
         gccgccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggccacggc                                                                                        <  1:3146754/101‑1 (MQ=255)
           cgccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggccacggcca                                                                                      >  2:1546695/1‑101 (MQ=255)
           cgccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggccacggcca                                                                                      >  2:1882858/1‑101 (MQ=255)
           cgccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggccacggcca                                                                                      <  2:4379223/101‑1 (MQ=255)
           cgccCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCAGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTcggccacggcca                                                                                      >  2:1987658/1‑101 (MQ=255)
             ccccGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGc                                                                                    >  1:2137177/1‑101 (MQ=255)
              cccGGCCGCAAGCGCCAGCACCGGCAGGACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCGCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGcc                                                                                   >  2:672346/1‑100 (MQ=255)
              cccGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCa                                                                                   >  2:1379065/1‑101 (MQ=255)
              cccGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCa                                                                                   <  1:1638848/101‑1 (MQ=255)
              cccGGCCCCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCa                                                                                   <  2:2864619/101‑1 (MQ=255)
               gatgCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCAc                                                                                  >  2:1306583/4‑101 (MQ=255)
                 ggCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCAccc                                                                                >  2:1444437/1‑101 (MQ=255)
                 ggCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCAccc                                                                                >  1:3377890/1‑101 (MQ=255)
                 ggCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCAccc                                                                                <  1:1042869/101‑1 (MQ=255)
                  gCCGCAAGCGCCAGCACCGGCAGCCCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCa                                                                               <  2:2105091/101‑1 (MQ=255)
                  gCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCa                                                                               <  2:1109151/101‑1 (MQ=255)
                  gCCCCAAGCGCCAGCCCCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCa                                                                               <  1:1977092/101‑1 (MQ=255)
                    cGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGt                                                                             <  2:1044836/101‑1 (MQ=255)
                     gCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTc                                                                            <  2:2671870/101‑1 (MQ=255)
                      cAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCg                                                                           >  2:636426/1‑101 (MQ=255)
                       aaGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCgg                                                                          >  2:575865/1‑101 (MQ=255)
                        aGCCCCAGCCCCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCCCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGt                                                                         <  2:288500/101‑1 (MQ=255)
                          cgcCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGc                                                                       >  2:4317866/1‑101 (MQ=255)
                             caacACCGGCGGCCCCCAGCCGGGCGGCGCCGGAGGAAAGGTCGGCCCACCCGCCAGACCCCCGACGAGCAAGGCCCCGGCCAGCACCCAGTTGGTGCCCa                                                                    <  2:3663764/98‑1 (MQ=255)
                             cAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCa                                                                    <  1:4154034/101‑1 (MQ=255)
                                 aCCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGccc                                                                >  1:1799459/1‑101 (MQ=255)
                                 aCCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGccc                                                                >  1:1027457/1‑101 (MQ=255)
                                 aCCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTAGGTGCCCAGccc                                                                >  2:3903180/1‑101 (MQ=255)
                                  ccGGCAGCACCCAGCCGGGCGGGGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGcccc                                                               <  1:420838/101‑1 (MQ=255)
                                  ccGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGcccc                                                               >  1:398668/1‑101 (MQ=255)
                                   cGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCa                                                              >  2:2199001/1‑101 (MQ=255)
                                     gcagccCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATc                                                            <  1:2050338/101‑1 (MQ=255)
                                      cagcaCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCa                                                           <  1:2188444/101‑1 (MQ=255)
                                        gcccccAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGc                                                         <  1:1882982/98‑1 (MQ=255)
                                        gcaCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGc                                                         >  1:2618514/1‑101 (MQ=255)
                                         caCCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCACGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGcc                                                        <  1:3656158/101‑1 (MQ=255)
                                           cccTGCCGGCCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGcccg                                                      >  2:169371/1‑99 (MQ=255)
                                            ccAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCaga                                                     >  2:3436099/1‑101 (MQ=255)
                                            ccAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCaga                                                     <  2:3377653/101‑1 (MQ=255)
                                             cAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCagag                                                    >  1:2981046/1‑101 (MQ=255)
                                              aGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAgc                                                   >  1:632415/1‑101 (MQ=255)
                                              aGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAgc                                                   >  1:3239948/1‑101 (MQ=255)
                                              aGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAgc                                                   >  2:4130334/1‑101 (MQ=255)
                                               gCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCACCATCAGCCAGAgcg                                                  >  2:526903/1‑101 (MQ=255)
                                                ccGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAgcgc                                                 <  1:3882727/101‑1 (MQ=255)
                                                ccGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAgcgc                                                 >  1:359489/1‑101 (MQ=255)
                                                 cGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGcgcg                                                >  2:814334/1‑101 (MQ=255)
                                                   ggcggcGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCggg                                              >  1:4293144/1‑101 (MQ=255)
                                                    gcggcgCGGGCAGAAAGGTCTGCCCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGc                                             <  1:1534474/101‑1 (MQ=255)
                                                     cggcgCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGcc                                            >  2:233303/1‑101 (MQ=255)
                                                      ggcgCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGccc                                           >  1:840398/1‑101 (MQ=255)
                                                      ggcgCGGGCAGAAAGGTCTGCCCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGccc                                           <  1:809042/101‑1 (MQ=255)
                                                       gcgcGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGcccc                                          >  1:2829925/1‑101 (MQ=255)
                                                           gggCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCa                                      >  1:1434928/1‑101 (MQ=255)
                                                           gggCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCa                                      >  2:4250669/1‑101 (MQ=255)
                                                           gggCAGAAAGGTCGGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCa                                      <  2:3924114/101‑1 (MQ=255)
                                                             gCAGAAAGGTCTGCCCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGa                                    <  1:1558927/101‑1 (MQ=255)
                                                              cAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGAc                                   >  1:3273733/1‑101 (MQ=255)
                                                               aGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGAcc                                  <  2:804309/101‑1 (MQ=255)
                                                                gAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCCCCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGAccc                                 <  1:709339/101‑1 (MQ=255)
                                                                gAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGAccc                                 >  2:118753/1‑101 (MQ=255)
                                                                 aaaGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCa                                >  2:1966186/1‑101 (MQ=255)
                                                                  aaGGTCTGCGCCCCCGCCAGACCCGCGACGAACACGGCCACGGCCAGCACCAAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAg                               >  2:2496377/1‑101 (MQ=255)
                                                                   aGGTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAgc                              >  2:1805802/1‑101 (MQ=255)
                                                                    ggTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAgcg                             <  1:2872073/101‑1 (MQ=255)
                                                                    ggTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAgcg                             >  2:205596/1‑101 (MQ=255)
                                                                    ggTCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAgcg                             <  1:2731523/101‑1 (MQ=255)
                                                                      tCTGCGCCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAgcggc                           <  2:4020201/101‑1 (MQ=255)
                                                                          cgcCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCa                       >  2:2708860/1‑101 (MQ=255)
                                                                          cgcCCCCGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCa                       <  2:2386493/101‑1 (MQ=255)
                                                                               ccGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCATAAcc                  >  1:1687407/1‑101 (MQ=255)
                                                                                cGCCAGCCCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccg                 <  1:356047/101‑1 (MQ=255)
                                                                                cGCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccg                 >  2:2830009/1‑101 (MQ=255)
                                                                                 gCCAGCCCCGCGACGAACTCGGCCACGGCCAGCACCAAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccgc                <  1:2496540/101‑1 (MQ=255)
                                                                                 gCCAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccgc                >  1:51061/1‑101 (MQ=255)
                                                                                  ccAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccgcc               >  2:330303/1‑101 (MQ=255)
                                                                                   cAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccgccg              <  2:2186820/101‑1 (MQ=255)
                                                                                   cAGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAAccgccg              >  1:2293686/1‑101 (MQ=255)
                                                                                    aGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACcgccgc             >  2:849166/1‑101 (MQ=255)
                                                                                    aGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACcgccgc             >  2:3938035/1‑101 (MQ=255)
                                                                                    aGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACcgccgc             >  1:2373409/1‑101 (MQ=255)
                                                                                    aGACCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACcgccgc             >  2:1177236/1‑101 (MQ=255)
                                                                                     gccccGCGACGACCTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCgccgcc            <  2:2137038/99‑1 (MQ=255)
                                                                                      gcccGCGACGAACTGGGCCACGGCCAGCCCCCAGTCGTTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCCGAACCGCCGCCa           <  1:2419769/100‑1 (MQ=255)
                                                                                      aCCCGCGACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCa           >  2:4343185/1‑101 (MQ=255)
                                                                                         cgcgACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCa        >  2:4061203/1‑101 (MQ=255)
                                                                                         cgcgACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCa        >  1:95300/1‑101 (MQ=255)
                                                                                         cgcgACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCa        >  1:4175994/1‑101 (MQ=255)
                                                                                          gcgACGAACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGACGCCAGCAc       <  1:1379162/101‑1 (MQ=255)
                                                                                            gacgaACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCAccc     >  2:353981/1‑101 (MQ=255)
                                                                                              cgaACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCg   <  2:4376527/101‑1 (MQ=255)
                                                                                              cgaACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCg   >  1:4047022/1‑101 (MQ=255)
                                                                                               gaACTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCgg  >  1:825235/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GACAGCACCGCCGCCCCGGCCGCAAGCGCCAGCACCGGCAGCACCCAGCCGGGCGGCGCGGGCAGAAAGGTCTGCGCCCCCGCCAGACCCGCGACGAAGTCGGCCACGGCCAGCACCCAGTCGGTGCCCAGCCCCATCAGCCAGAGCGCGGGCCCCGCCAGACCCAGCGGCGCCAGAACCGCCGCCAGCACCCCGG  >  NC_014034/2531615‑2531810

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG