Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,549,153 G→A 100% G56S (GGC→AGC)  tonB → protein TonB

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,549,1530G→A100.0% 623.3 164G56S (GGC→AGC) tonBprotein TonB
Reads supporting (aligned to +/- strand):  new base (88/76):  ref base (0/0):  total (88/76)

Only 100 of 187 total aligned reads displayed.

CGCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACGGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCGA  >  NC_014034/2549055‑2549250
                                                                                                  |                                                                                                 
cGCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGc                                                                                                 <  2:881787/101‑1 (MQ=255)
  cTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCt                                                                                               >  1:1018047/1‑101 (MQ=255)
  cTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCt                                                                                               >  2:1636689/1‑101 (MQ=255)
  cTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCt                                                                                               <  1:999075/101‑1 (MQ=255)
  cTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCt                                                                                               <  2:2107974/101‑1 (MQ=255)
   tGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTc                                                                                              >  2:1531138/1‑101 (MQ=255)
   tGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTc                                                                                              >  1:528527/1‑101 (MQ=255)
    ggCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGGTCTGGACAGCCTCg                                                                                             >  2:4154000/1‑101 (MQ=255)
    ggCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCg                                                                                             <  1:2422538/101‑1 (MQ=255)
    ggCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCg                                                                                             <  2:2169352/101‑1 (MQ=255)
       gcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAg                                                                                          >  1:1340960/1‑101 (MQ=255)
       gcTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAg                                                                                          <  1:3640691/101‑1 (MQ=255)
        cTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGc                                                                                         >  2:1815143/1‑101 (MQ=255)
        cTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGc                                                                                         >  2:2163877/1‑101 (MQ=255)
         tGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCg                                                                                        >  1:1007844/1‑101 (MQ=255)
          gCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGa                                                                                       >  1:310001/1‑101 (MQ=255)
           cATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGAt                                                                                      <  2:2297013/101‑1 (MQ=255)
           cATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGAt                                                                                      >  2:4003535/1‑101 (MQ=255)
           cATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCCAt                                                                                      >  2:1372069/1‑101 (MQ=255)
            aTGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATc                                                                                     >  1:1624565/1‑101 (MQ=255)
            aTGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATc                                                                                     >  2:1676871/1‑101 (MQ=255)
            aTGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATc                                                                                     >  1:3435309/1‑101 (MQ=255)
            aTGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATc                                                                                     >  1:4045612/1‑101 (MQ=255)
            aTGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATc                                                                                     <  1:3752123/101‑1 (MQ=255)
              gCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgc                                                                                   >  1:3960925/1‑101 (MQ=255)
                ggTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgccg                                                                                 <  1:388143/101‑1 (MQ=255)
                ggTCGCGGGTTCCGCGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGTCAGCTGGCGCGGGCGGCGAGGCTCTGGACGGCCTCCAAGCTATcctcc                                                                                 >  2:3689426/1‑97 (MQ=255)
                 gTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgccgc                                                                                <  2:1285924/101‑1 (MQ=255)
                 gTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATcgccgc                                                                                <  1:2469187/101‑1 (MQ=255)
                    gcgGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGGGGGGGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGAc                                                                             <  1:1280669/101‑1 (MQ=255)
                    gcgGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGAc                                                                             >  2:1455193/1‑101 (MQ=255)
                     cgGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGAcc                                                                            <  2:4390556/101‑1 (MQ=255)
                      ggTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGGCCt                                                                           >  2:2566786/1‑101 (MQ=255)
                      ggTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCt                                                                           <  2:2547595/101‑1 (MQ=255)
                      ggTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCt                                                                           >  2:750650/1‑101 (MQ=255)
                       gTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCtt                                                                          >  2:3171984/1‑101 (MQ=255)
                        tttCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtc                                                                         <  1:1381241/101‑1 (MQ=255)
                         ttCGGTGCTATCGCTGACCGAATCCTCCCCCCCGGCGGTGGCGGCGGCGGGCGCGGGCGGCGATGGTCTGGACAGCCTCGACACGATTGCCGCGACCTtcc                                                                        >  2:422798/1‑100 (MQ=255)
                         ttCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcg                                                                        <  1:1412063/101‑1 (MQ=255)
                         ttCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcg                                                                        <  1:2289714/101‑1 (MQ=255)
                          tCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgt                                                                       <  2:2501192/101‑1 (MQ=255)
                           ccGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGGGGCGAGGATCTCGACAGCCTCGAAGCGATCGCGGCGACCTtcgtc                                                                      >  1:3853193/1‑101 (MQ=255)
                            cGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtcg                                                                     <  2:2737925/101‑1 (MQ=255)
                            cGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtcg                                                                     >  1:2171806/1‑101 (MQ=255)
                            cGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTtcgtcg                                                                     <  1:1081167/101‑1 (MQ=255)
                             gTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGTTCTGGACAGCCTCGAAGCGATCGGCGCGACCTtcgtcgg                                                                    >  1:1151215/1‑100 (MQ=255)
                              tGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGAt                                                                   <  2:547317/101‑1 (MQ=255)
                              tGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGAt                                                                   >  2:492112/1‑101 (MQ=255)
                               gCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATc                                                                  >  1:606376/1‑101 (MQ=255)
                                    cGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGgcg                                                             <  2:913998/101‑1 (MQ=255)
                                      cAGACCGGGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgcc                                                           <  1:1878553/101‑1 (MQ=255)
                                       aGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgccg                                                          >  2:3485266/1‑101 (MQ=255)
                                       aGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgccg                                                          <  2:3436159/101‑1 (MQ=255)
                                       aGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgccg                                                          <  1:3615299/101‑1 (MQ=255)
                                       aGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGcgccg                                                          >  1:740758/1‑101 (MQ=255)
                                         aCCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCa                                                        >  2:1988828/1‑101 (MQ=255)
                                            gAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGAt                                                     >  1:1760984/1‑101 (MQ=255)
                                             aGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATc                                                    <  1:4151222/101‑1 (MQ=255)
                                               gccgccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCa                                                  >  2:3066022/1‑101 (MQ=255)
                                               gccgccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCa                                                  >  2:1180646/1‑101 (MQ=255)
                                               gccgccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCa                                                  <  1:1802719/101‑1 (MQ=255)
                                               gccgccCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCa                                                  <  1:3265316/101‑1 (MQ=255)
                                                   cccccGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTg                                              <  2:309966/101‑1 (MQ=255)
                                                    ccccGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgc                                             <  2:4048080/101‑1 (MQ=255)
                                                     cccGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcc                                            <  2:248538/101‑1 (MQ=255)
                                                         cggcgCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgag                                        >  2:3214517/1‑101 (MQ=255)
                                                          ggcgCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagc                                       >  2:2108944/1‑101 (MQ=255)
                                                           gcgcTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagcc                                      >  2:3419200/1‑101 (MQ=255)
                                                           gcgcTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagcc                                      >  2:2573922/1‑101 (MQ=255)
                                                            cgcTGGTGGCGGCGGGCGCCGGCGCCGAGGCTCTGGGCAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagccc                                     <  2:4378194/101‑1 (MQ=255)
                                                             gcTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagcccg                                    >  1:4218650/1‑101 (MQ=255)
                                                             gcTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagcccg                                    <  2:1325135/101‑1 (MQ=255)
                                                              cTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTgcccgagcccga                                   <  2:2667852/101‑1 (MQ=255)
                                                                 gtggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGAt                                <  2:3413983/101‑1 (MQ=255)
                                                                  tggCGGCGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATg                               >  1:14003/1‑101 (MQ=255)
                                                                   ggcggcGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATgg                              >  2:2356701/1‑101 (MQ=255)
                                                                   ggcggcGGGCGCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATgg                              >  2:1096631/1‑101 (MQ=255)
                                                                      ggcgggcgCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGgcgc                           >  1:2107839/1‑101 (MQ=255)
                                                                      ggcgggcgCGGGCGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGgcgc                           >  2:2439125/1‑101 (MQ=255)
                                                                       gcgggcgcgggcGGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCt                          <  1:3333493/101‑1 (MQ=255)
                                                                        cgggcgcgggcgGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTg                         >  1:3264664/1‑101 (MQ=255)
                                                                         gggcgcgggcgGCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGc                        >  2:4313644/1‑101 (MQ=255)
                                                                             gcgggcggCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGAGCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCt                    <  2:3855697/101‑1 (MQ=255)
                                                                               gggcggCGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGa                  >  1:2107716/1‑101 (MQ=255)
                                                                                gggggggAGGCTCCGGACCGCCCCGAAGGCACCCCCGCGCCCTTCGGCGATCTGGCCCCGCAGCTCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGAc                 <  2:1613308/95‑1 (MQ=255)
                                                                                ggcggcGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGAc                 >  1:3516440/1‑101 (MQ=255)
                                                                                ggcggcGAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGAc                 >  2:3346898/1‑101 (MQ=255)
                                                                                  cggcgAGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACcg               <  1:1676982/101‑1 (MQ=255)
                                                                                       aGGATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCg          >  2:2866343/1‑101 (MQ=255)
                                                                                        ggATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCgg         <  1:4089445/101‑1 (MQ=255)
                                                                                        ggATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCgg         >  2:1017058/1‑101 (MQ=255)
                                                                                         gATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCggg        >  1:2840938/1‑100 (MQ=255)
                                                                                         gATCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCggg        >  1:845306/1‑100 (MQ=255)
                                                                                          aTCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTg       >  2:66282/1‑101 (MQ=255)
                                                                                          aTCTGGACAGCCTCGAAGCAATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTg       <  1:422838/101‑1 (MQ=255)
                                                                                           tCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGa      <  2:1760865/101‑1 (MQ=255)
                                                                                           tCTGGACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGa      <  2:14131/101‑1 (MQ=255)
                                                                                              ggACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCg   >  1:4125038/1‑101 (MQ=255)
                                                                                              ggACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCg   >  1:1945907/1‑101 (MQ=255)
                                                                                              ggACAGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCg   <  2:1350109/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CGCTGGCGCTGCATGCGGTCGCGGTTTCCGTGCTCTCGCAGACCGAGGCCGCCCCCGCGGCGCTGGTGGCGGCGGGCGCGGGCGGCGAGGATCTGGACGGCCTCGAAGCGATCGCCGCGACCTTCGTCGATCTGGCGCCGCAGATCCAGCTGCCCGAGCCCGAGATGGCGCTGCCGCTGACCGCGCCGGTGATCGA  >  NC_014034/2549055‑2549250

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG