Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,554,356 T→C 100% N96D (AAC→GAC)  mraW ← S‑adenosyl‑methyltransferase MraW

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,554,3560T→C100.0% 830.9 222N96D (AAC→GAC) mraWS‑adenosyl‑methyltransferase MraW
Reads supporting (aligned to +/- strand):  new base (101/121):  ref base (0/0):  total (101/121)

Only 100 of 231 total aligned reads displayed.

CATCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTTCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAGGGC  >  NC_014034/2554258‑2554453
                                                                                                  |                                                                                                 
cATCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCa                                                                                                 <  1:2437584/101‑1 (MQ=255)
    cGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGccc                                                                                             >  1:2944789/1‑101 (MQ=255)
     gCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCt                                                                                            >  2:2976661/1‑101 (MQ=255)
      cATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCtt                                                                                           <  1:3432616/101‑1 (MQ=255)
         gTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCgg                                                                                        <  2:35586/101‑1 (MQ=255)
             aaGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGgcgc                                                                                    >  2:125063/1‑101 (MQ=255)
               gggCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGa                                                                                  >  2:2072870/1‑101 (MQ=255)
                ggCCCGTCGCGCAGAAAGGAAAAGCCTCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGACCCAGCACCACCCCGTCAAGCCCTTCGGGCGCGAt                                                                                 <  2:646294/101‑1 (MQ=255)
                ggCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAt                                                                                 <  1:1427296/101‑1 (MQ=255)
                  cccGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCCGCACCACCCCGTCCAGCCCTTCGGGCGCGAtct                                                                               >  1:3798227/1‑101 (MQ=255)
                  cccGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCACTTCGGGCGCGAtct                                                                               >  1:1899579/1‑101 (MQ=255)
                   ccGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtctc                                                                              <  1:760894/101‑1 (MQ=255)
                   ccGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGAtctc                                                                              <  1:4267282/101‑1 (MQ=255)
                     gTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcgg                                                                            >  1:1849493/1‑101 (MQ=255)
                      tCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcggc                                                                           <  2:4072516/101‑1 (MQ=255)
                      tCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcggc                                                                           >  1:2590117/1‑101 (MQ=255)
                        gcgcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTcggcgg                                                                         <  1:1731907/101‑1 (MQ=255)
                         cgcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCggcggc                                                                        <  2:3445735/101‑1 (MQ=255)
                         cgcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCggcggc                                                                        <  2:862077/101‑1 (MQ=255)
                          gcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGgcggcg                                                                       >  2:602168/1‑101 (MQ=255)
                          gcAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGgcggcg                                                                       >  2:3479469/1‑101 (MQ=255)
                            aGAAAGGAAAATCCGCGCGCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGAc                                                                     <  1:2654252/101‑1 (MQ=255)
                            aGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGAc                                                                     >  2:1115153/1‑101 (MQ=255)
                            aGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGAc                                                                     >  1:3641438/1‑101 (MQ=255)
                             gAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACg                                                                    >  2:1494241/1‑101 (MQ=255)
                             gAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACg                                                                    <  1:1702905/101‑1 (MQ=255)
                             gAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACg                                                                    <  1:475508/101‑1 (MQ=255)
                             gAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACg                                                                    >  1:4055029/1‑101 (MQ=255)
                              aaaGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACgg                                                                   >  2:3887668/1‑101 (MQ=255)
                              aaaGAAAAAGCCGCGTCCGGCTGGATGGAGCGGAATGGAGGACCCCCCCGGATCAGGCACC.CCCCGGCCCGGCCCTCGGGCGCGATCTCGGCGGCGACGGc                                                                  <  1:2842693/101‑1 (MQ=255)
                               atctaaaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGc                                                                  <  2:2597920/97‑1 (MQ=255)
                               aaGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGc                                                                  >  1:1879544/1‑101 (MQ=255)
                               aaGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGc                                                                  <  1:724874/101‑1 (MQ=255)
                                aGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCa                                                                 <  1:658741/101‑1 (MQ=255)
                                aGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCa                                                                 <  1:2400143/101‑1 (MQ=255)
                                 ggAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCAt                                                                <  1:1025859/101‑1 (MQ=255)
                                  gAAAAGCTGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATc                                                               <  2:2792561/101‑1 (MQ=255)
                                  gAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATc                                                               <  1:1677802/101‑1 (MQ=255)
                                  gAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATc                                                               <  1:2989348/101‑1 (MQ=255)
                                  gAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATc                                                               <  1:2207586/101‑1 (MQ=255)
                                   gaaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGGCACGCCCAGATCCAGCACCGCCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCg                                                              <  2:1131162/100‑1 (MQ=255)
                                    aaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGa                                                             <  2:241535/101‑1 (MQ=255)
                                    aaaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGa                                                             >  2:3084854/1‑101 (MQ=255)
                                     aaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAg                                                            >  1:4133484/1‑101 (MQ=255)
                                     aaGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAg                                                            >  1:1496754/1‑101 (MQ=255)
                                        ccGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGtt                                                         >  2:1425229/1‑101 (MQ=255)
                                        ccGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGtt                                                         <  1:2084648/101‑1 (MQ=255)
                                           cgcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGa                                                      <  1:388542/101‑1 (MQ=255)
                                            gcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaa                                                     <  1:3530989/101‑1 (MQ=255)
                                            gcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaa                                                     >  2:1079886/1‑101 (MQ=255)
                                            gcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaa                                                     <  1:2330193/101‑1 (MQ=255)
                                            gcTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaa                                                     >  2:3958635/1‑101 (MQ=255)
                                             cTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaaa                                                    >  1:107383/1‑101 (MQ=255)
                                             cTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaaa                                                    <  1:401225/101‑1 (MQ=255)
                                              tCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGaaaa                                                   <  2:2089229/101‑1 (MQ=255)
                                                ggCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAgg                                                 >  1:316382/1‑101 (MQ=255)
                                                 gCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGgt                                                >  2:4395891/1‑101 (MQ=255)
                                                    tGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGtt                                             <  2:813273/101‑1 (MQ=255)
                                                    tGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGtt                                             <  2:272645/101‑1 (MQ=255)
                                                    tGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGtt                                             <  2:543896/101‑1 (MQ=255)
                                                     gATCGATCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtc                                            <  2:2350163/101‑1 (MQ=255)
                                                      aTCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtct                                           <  2:2180337/101‑1 (MQ=255)
                                                      aTCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtct                                           <  1:4335599/101‑1 (MQ=255)
                                                      aTCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtct                                           <  1:3618885/101‑1 (MQ=255)
                                                       tCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTtctc                                          <  1:2885335/101‑1 (MQ=255)
                                                         gAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGa                                        >  1:2253643/1‑101 (MQ=255)
                                                          aGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGac                                       >  1:1155119/1‑101 (MQ=255)
                                                          aGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGac                                       <  2:3641185/101‑1 (MQ=255)
                                                             tGCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccag                                    <  2:3139292/101‑1 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCCCCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagc                                   <  2:1593648/101‑1 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGacccgc                                   >  2:3776475/1‑101 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagc                                   <  2:1641291/101‑1 (MQ=255)
                                                              gCATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagc                                   >  2:1269587/1‑101 (MQ=255)
                                                               cATCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagca                                  <  1:4085388/101‑1 (MQ=255)
                                                                aTCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcac                                 <  2:4133224/101‑1 (MQ=255)
                                                                 tCGACGACACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcacc                                <  1:4176592/101‑1 (MQ=255)
                                                                   gacgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcaccag                              >  2:2645087/1‑101 (MQ=255)
                                                                    acgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcaccagc                             <  1:3178434/101‑1 (MQ=255)
                                                                    acgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcaccagc                             >  1:2809770/1‑101 (MQ=255)
                                                                    acgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcaccagc                             >  2:2392733/1‑101 (MQ=255)
                                                                    acgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGaccagcaccagc                             >  2:1905913/1‑101 (MQ=255)
                                                                     cgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGcc                            <  1:2671617/101‑1 (MQ=255)
                                                                     cgacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGcc                            <  1:2155870/101‑1 (MQ=255)
                                                                      gacACGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCg                           <  1:3035299/101‑1 (MQ=255)
                                                                        cacGCCCAGATCCAGCACCCCCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCt                         <  2:501301/101‑1 (MQ=255)
                                                                         acGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTc                        >  2:1213403/1‑101 (MQ=255)
                                                                          cGCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCg                       >  1:566203/1‑101 (MQ=255)
                                                                           gCCCAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGc                      >  1:3938850/1‑101 (MQ=255)
                                                                              cAGATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGa                   >  2:1996569/1‑101 (MQ=255)
                                                                                gATCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAAc                 >  2:2616151/1‑101 (MQ=255)
                                                                                 aTCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACt                >  2:721131/1‑101 (MQ=255)
                                                                                 aTCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACt                <  2:4449100/101‑1 (MQ=255)
                                                                                 aTCCAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACt                >  1:1423623/1‑101 (MQ=255)
                                                                                 aTCCAGCACAACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACt                >  2:418937/1‑101 (MQ=255)
                                                                                    cAGCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTcgc             <  2:2452810/101‑1 (MQ=255)
                                                                                      gCACCACCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTcgccg           >  1:92437/1‑101 (MQ=255)
                                                                                       caccccCCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTcgccgc          <  1:1318523/101‑1 (MQ=255)
                                                                                           accCCGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCa      >  2:3213015/1‑101 (MQ=255)
                                                                                             cccGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAgg    >  1:3315567/1‑101 (MQ=255)
                                                                                             cccGTCCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAgg    <  2:1724564/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CATCCGCATGTCCAAGGGCCCGTCGCGCAGAAAGGAAAAGCCGCGCTCGGCCTGATCGAGCTGCATCGACGACACGCCCAGATCCAGCACCACCCCGTTCAGCCCTTCGGGCGCGATCTCGGCGGCGACGGCATCGAGGTTCGAAAAGGTGTTCTCGACCAGCACCAGCCGCTCGCCGAACTCGCCGCGCCAGGGC  >  NC_014034/2554258‑2554453

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG