Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,559,923 G→C 100% intergenic (‑199/+71) ispZ ← / ← ftsY intracellular septation protein A/cell division protein FtsY

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,559,9230G→C100.0% 398.4 111intergenic (‑199/+71)ispZ/ftsYintracellular septation protein A/cell division protein FtsY
Reads supporting (aligned to +/- strand):  new base (98/11):  ref base (0/2):  total (98/13)

Only 100 of 155 total aligned reads displayed.

TCGCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGGGGGGGGCAGCGCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCG  >  NC_014034/2559825‑2560021
                                                                                                      |                                                                                                  
tcgCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAgcgg                                                                                                  >  1:1147050/1‑101 (MQ=255)
 nnnccNGTGCGGTTTCCNGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAgcggg                                                                                                 <  2:682917/98‑1 (MQ=255)
 cgCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAgcggg                                                                                                 <  2:4327877/101‑1 (MQ=255)
 cgCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAgcggg                                                                                                 <  2:1732531/101‑1 (MQ=255)
 cgCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAgcggg                                                                                                 >  1:2222572/1‑101 (MQ=255)
    ccGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAgcgggggg                                                                                              <  1:1024005/101‑1 (MQ=255)
    ccGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAgcgggggg                                                                                              >  2:1966563/1‑101 (MQ=255)
    ccGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAgcgggggg                                                                                              <  1:2241517/101‑1 (MQ=255)
        tGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAgc                                                                                          <  2:1245174/101‑1 (MQ=255)
          cGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAgcgc                                                                                        >  1:1788032/1‑101 (MQ=255)
          cGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAgcgc                                                                                        <  2:3704144/101‑1 (MQ=255)
            gTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCGccc                                                                                      >  1:318898/1‑101 (MQ=255)
             tttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCGcccc                                                                                     <  1:4258795/101‑1 (MQ=255)
              ttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCGccccc                                                                                    >  1:4395736/1‑101 (MQ=255)
               tCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCGcccccc                                                                                   <  1:2148014/101‑1 (MQ=255)
               tCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCGcccccc                                                                                   <  2:2135245/101‑1 (MQ=255)
                  tGACAGGGTTGTGTCCTTCGCGCCGCGGCGGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCGccccccgaa                                                                                <  1:3535661/101‑4 (MQ=255)
                  tGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCGccccccgaa                                                                                >  2:4403496/1‑98 (MQ=255)
                    aCAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCGcccccccaagc                                                                              >  2:679234/1‑97 (MQ=255)
                    aCAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAgc                                                                             >  2:2585130/1‑101 (MQ=255)
                    aCAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAgc                                                                             >  2:2062046/1‑101 (MQ=255)
                      aGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAgcgc                                                                           >  2:1405844/1‑101 (MQ=255)
                       ggATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGcc                                                                          >  2:3282853/1‑101 (MQ=255)
                        gATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCt                                                                         >  1:2480041/1‑101 (MQ=255)
                           tgtgTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAg                                                                      >  2:3064345/1‑101 (MQ=255)
                           tgtgTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAg                                                                      >  2:1457468/1‑101 (MQ=255)
                            gtgtCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAgg                                                                     >  2:3408338/1‑101 (MQ=255)
                            gtgtCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAgg                                                                     >  1:2920343/1‑101 (MQ=255)
                            gtgtCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGGT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCTAAGCGCCTGAgg                                                                     >  2:376368/1‑101 (MQ=255)
                             tgtCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGgc                                                                    >  1:3607436/1‑101 (MQ=255)
                                ccTTCGCGCCGCCGCGGGCCCGCTTTTCCTTGGGCGAAACGCGGGGGGGTT.G.CGCG.TCA.GCGCAAGGGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGcgcg                                                                 <  2:2017553/101‑1 (MQ=255)
                                   tCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCgcggcg                                                              >  2:2603119/1‑101 (MQ=255)
                                    cgcgCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGcggcgg                                                             >  2:2039006/1‑101 (MQ=255)
                                     gcgcCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCggg                                                            >  2:2281332/1‑101 (MQ=255)
                                      cgccgcAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGc                                                           >  1:2309492/1‑101 (MQ=255)
                                       gccgcAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGGG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGAGCCTGAGGCGCGGCGGGCt                                                          >  1:3744212/1‑101 (MQ=255)
                                       gccgcAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCt                                                          >  2:3823732/1‑101 (MQ=255)
                                        ccgcAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCtt                                                         >  2:1752995/1‑101 (MQ=255)
                                          gcagcaGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgc                                                       >  2:2139479/1‑101 (MQ=255)
                                           cagcagGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTtcc                                                      >  2:4296613/1‑101 (MQ=255)
                                           cagcagGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgcc                                                      >  2:3281730/1‑101 (MQ=255)
                                           cagcagGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgcc                                                      >  2:1240363/1‑101 (MQ=255)
                                           cagcagGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCGTgcc                                                      >  2:1702752/1‑101 (MQ=255)
                                            agcagGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGGCTGAGGCGCGGCGGGCGTgccc                                                     >  2:2875900/1‑101 (MQ=255)
                                            agcagGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTtccc                                                     >  1:2078987/1‑101 (MQ=255)
                                            agcagGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgccc                                                     >  1:2686290/1‑101 (MQ=255)
                                            agcagGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCCTgccc                                                     >  2:619532/1‑101 (MQ=255)
                                             gcagGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgcccc                                                    >  1:2870217/1‑100 (MQ=255)
                                               agGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgcccgcc                                                  >  1:3419082/1‑101 (MQ=255)
                                                 cccggtttttCCTGGGGCGAACCG.CGGGGGGTT.GCCGCG.TCA.GC.CAAGGGGGGGGCCCGCCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCa                                                <  1:2125215/95‑1 (MQ=16)
                                                   cctttttcCCTGGGGCAAAGGGCGGGGGGGTG.G.CGCG.ACG.GCGC.AGGGGGGGGCCGGCCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAAc                                              <  1:2090991/97‑1 (MQ=12)
                                                    cccTTTTCCTTGGGGCAAACCGGGGGGGGTTGG.CGGG.GCG.GCG.AAGGGGGGGGGGGCCCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAAc                                              <  1:4207462/99‑1 (MQ=12)
                                                     tttttcCCTGGGGCGAACCGCGGGGGGGTG.G.CCCG.CCC.GCGCAGGGGGGGGGCCGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGa                                            <  1:661131/99‑1 (MQ=18)
                                                     ctttttCCCTGGGGAAAACGGCGGGGGGGT.G..GCGCTCC.GCCCCAGGGGGGGGGCGGCCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACg                                             <  2:3526928/99‑1 (MQ=12)
                                                       ttttCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGGTTGCCCGCCCAACGAAg                                          >  2:909758/1‑101 (MQ=255)
                                                         ttCCTGGGGCGAAACGCGGGGGGGTG.G.CGCG.CCA.GCGCAGGGGGGGGGCAGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGgc                                        <  2:497770/101‑1 (MQ=25)
                                                          tCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGgcc                                       >  2:1413591/1‑100 (MQ=255)
                                                           ccTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGgcgc                                      >  1:3647158/1‑101 (MQ=255)
                                                           ccTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGgcgc                                      >  1:295467/1‑101 (MQ=255)
                                                            ctggggCGAAACGCGGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCa                                     <  2:2660677/98‑1 (MQ=25)
                                                            cTTGGGCAGACCGCGGGGGTGTG.G.CCCG.CCG.GGGCAGGTCGGGTGCGGCG.CCCCTCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCa                                     <  2:3670982/101‑1 (MQ=9)
                                                              tGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGGGCAac                                   >  1:272527/1‑101 (MQ=255)
                                                               gggCGACCCGCGGGGGGGTG.G.GGCG.CCA.GCG.AAGGGGGGGGCCGGCCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAacc                                  <  2:3494889/101‑1 (MQ=18)
                                                               gggCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAacc                                  >  2:1095020/1‑101 (MQ=255)
                                                                 gCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAaccac                                >  1:1011932/1‑101 (MQ=255)
                                                                  cGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGTATACCCACCCAAAGAAgggggcacaacc                               >  2:3870442/1‑91 (MQ=255)
                                                                   gAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCt                              >  1:4064087/1‑101 (MQ=255)
                                                                   gAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACCAAGGCGCAacccccc                              >  1:3161884/1‑100 (MQ=25)
                                                                    aaaCGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAAGCGCaaaacacct                             >  2:2326134/1‑94 (MQ=25)
                                                                     aaCGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCAAAGCGACTGAGGCGCGGCGGGCTTGCCCCCCCCACGAAGGCGCAACCACCCTc                            >  2:3037360/1‑101 (MQ=25)
                                                                      ccGCGGGGGGGTT.G.CGCG.CCC.GCGAAAGGGGGGGGCAGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCa                           <  2:2639171/100‑1 (MQ=25)
                                                                      aCGCGGGGGGGTT.G.CGCG.CCA.GCGAAGGGGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCa                           <  2:2507758/101‑1 (MQ=21)
                                                                        gcgggggggTT.G.CGCG.TAA.GCACAAGCGGGGGCCAGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGt                         <  1:2039143/98‑1 (MQ=25)
                                                                        gCCGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGt                         >  2:1049258/1‑101 (MQ=255)
                                                                        gCCGGGGGGTG.G.CGCT.GAA.GCGCAAGCGGGGGGCCGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGt                         <  2:1460613/101‑1 (MQ=21)
                                                                          tggggggTG.G.CGC..TCAGGGGCAGGCGGGGGGCAGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCg                       <  2:1158804/100‑1 (MQ=18)
                                                                          gggggggGG.G.GGCG.TCG.GGGCAGGGGGGGGGCAGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCg                       <  1:1240456/100‑1 (MQ=21)
                                                                          cGGGGGGTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCg                       >  2:2223762/1‑101 (MQ=255)
                                                                           ggggggTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCgg                      >  1:2362034/1‑101 (MQ=255)
                                                                           ggggggTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCgg                      >  2:585119/1‑101 (MQ=255)
                                                                           ggggggTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCgg                      >  2:2909432/1‑101 (MQ=255)
                                                                           ggggggTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCCTCAGTCgg                      >  2:3427144/1‑101 (MQ=255)
                                                                           ggggggTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAAACACCTTCAGTCgg                      >  2:2720587/1‑101 (MQ=255)
                                                                           ggggggTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACCAAGGCGCAACCACCTTCAGGCgg                      >  1:1966214/1‑101 (MQ=25)
                                                                            gggggTT.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCTGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGc                     >  1:3503245/1‑101 (MQ=25)
                                                                             gggtTT.G.CGCG.CGG.GCGCGGGGGGGGGCCCGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCt                    <  1:3408533/101‑1 (MQ=21)
                                                                              gggTC.G.CGCG.CCA.GCGAAGGGGGGGGGCAGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCtt                   <  2:2805839/101‑1 (MQ=25)
                                                                                 tt.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGa                >  1:1873376/1‑101 (MQ=255)
                                                                                  t.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCCCCTTCAGTCGGCCTCGAt               >  2:1063203/1‑101 (MQ=25)
                                                                                  t.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGAt               >  1:2819623/1‑101 (MQ=255)
                                                                                  t.G.CGCG.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGAt               >  2:2888625/1‑101 (MQ=255)
                                                                                    ggggcccgcA.GCGCGGGGGGGGCGCCCCCCCCCCCGGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCg                 <  1:2594336/93‑1 (MQ=21)
                                                                                       gcg.TCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCCTGGCCGCCCAACGAACGGGCAACCACCTTCAGTCGGCTTCGATccc            >  2:3990581/1‑101 (MQ=37)
                                                                                           tCA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccac         >  2:3561511/1‑101 (MQ=255)
                                                                                            cA.GCGCAAGGGGGGGGCA.CCCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacc        <  2:2939551/101‑1 (MQ=255)
                                                                                            cA.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacc        >  2:2701657/1‑101 (MQ=255)
                                                                                             a.GCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacca       >  2:2509464/1‑101 (MQ=255)
                                                                                                 gcAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgcg    >  2:413654/1‑101 (MQ=255)
                                                                                                  cAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgcgc   >  1:274309/1‑101 (MQ=255)
                                                                                                   aaGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCCGTCGGCTTCGATCCCCACCAGcgcg  >  1:1953908/1‑101 (MQ=255)
                                                                                                      |                                                                                                  
TCGCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAACGCCGGGGGGTT.G.CGCG.TCA.GCGCAAGGGGGGGGCAGCGCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCG  >  NC_014034/2559825‑2560021

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG