Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,560,404 A→G 100% F344L (TTC→CTC)  ftsY ← cell division protein FtsY

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,560,4040A→G100.0% 687.9 187F344L (TTC→CTC) ftsYcell division protein FtsY
Reads supporting (aligned to +/- strand):  new base (97/89):  ref base (0/1):  total (97/90)

Only 100 of 195 total aligned reads displayed.

CTCCATCAGATCGGCGCGGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAACGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGACCGCCGCGGCGCGGAACGTG  >  NC_014034/2560306‑2560501
                                                                                                  |                                                                                                 
 tccATCAGATCGGCGCGGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGa                                                                                                >  2:2561148/1‑101 (MQ=255)
  ccATCAGATCGGCGCGGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAg                                                                                               <  1:3772020/101‑1 (MQ=255)
     tCAGATCGGCGCGGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAACGAGGcc                                                                                            <  2:4426863/101‑1 (MQ=255)
      cAGATCGGCGCGGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCg                                                                                           >  2:2084613/1‑101 (MQ=255)
        gATCGGCGCGGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCggg                                                                                         <  1:4391355/101‑1 (MQ=255)
         aTCGGCGCGGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGt                                                                                        <  2:464379/101‑1 (MQ=255)
          tCGGCGCGGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTc                                                                                       >  2:3150088/1‑101 (MQ=255)
          tCGGCGCGGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTc                                                                                       <  2:3311282/101‑1 (MQ=255)
           cGGCGCGGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTcc                                                                                      <  1:915647/101‑1 (MQ=255)
           cGGCGCGGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTcc                                                                                      >  2:2251321/1‑101 (MQ=255)
           cGGCGCGGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTcc                                                                                      >  2:2947145/1‑101 (MQ=255)
            ggCGCGGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTccc                                                                                     >  1:3705485/1‑100 (MQ=255)
            ggCGCGGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCg                                                                                     <  1:2389818/101‑1 (MQ=255)
                cgGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGcccc                                                                                 >  2:3170844/1‑101 (MQ=255)
                   ttCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTc                                                                              <  1:2276067/101‑1 (MQ=255)
                    tCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCg                                                                             >  2:3527887/1‑101 (MQ=255)
                    tCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCg                                                                             <  2:2989699/101‑1 (MQ=255)
                    tCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCg                                                                             <  2:3902634/101‑1 (MQ=255)
                    tCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCg                                                                             >  2:4144608/1‑101 (MQ=255)
                    tCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCg                                                                             >  2:4250198/1‑101 (MQ=255)
                    tCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCg                                                                             <  1:361706/101‑1 (MQ=255)
                      tGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCggg                                                                           <  1:3946183/101‑1 (MQ=255)
                      tGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCggg                                                                           >  1:3224679/1‑101 (MQ=255)
                      tGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCggg                                                                           >  1:3466682/1‑101 (MQ=255)
                       gCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGgc                                                                          >  2:750805/1‑101 (MQ=255)
                       gCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGgc                                                                          >  1:2152307/1‑101 (MQ=255)
                         aGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGgcgc                                                                        >  2:2148949/1‑101 (MQ=255)
                            cgccCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGt                                                                     >  1:3792151/1‑101 (MQ=255)
                            cgccCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGt                                                                     >  2:2133717/1‑101 (MQ=255)
                             gccCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGtc                                                                    >  2:3737517/1‑101 (MQ=255)
                              cccGGCAGTGTCGATCATCAGCAGATTCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGCGCGAGGCCGGGCCCGCCCCCTCGGCCGCGGtct                                                                   >  2:1263666/1‑100 (MQ=255)
                               ccGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGtcat                                                                  >  1:4342751/1‑101 (MQ=255)
                               ccGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGtcat                                                                  <  2:1218434/101‑1 (MQ=255)
                                 ggCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGtcatca                                                                <  1:1069364/101‑1 (MQ=255)
                                 ggCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGgcatca                                                                >  1:1262134/1‑101 (MQ=255)
                                 ggCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGccatca                                                                >  2:850062/1‑101 (MQ=255)
                                  gCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCAc                                                               >  2:3624594/1‑101 (MQ=255)
                                   cAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCAcc                                                              <  1:386101/101‑1 (MQ=255)
                                   cAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCAcc                                                              >  1:3444461/1‑101 (MQ=255)
                                   cAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGGCCGCCCCCTCGGGCGCGGTCATCAcc                                                              >  1:1124983/1‑101 (MQ=255)
                                    aGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCg                                                             >  2:2716933/1‑101 (MQ=255)
                                     gtgtCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCgg                                                            >  1:3222244/1‑101 (MQ=255)
                                         cGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCAcc                                                        >  1:1006489/1‑101 (MQ=255)
                                          gATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCAccc                                                       >  2:3646838/1‑101 (MQ=255)
                                          gATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCAccc                                                       <  1:1435849/101‑1 (MQ=255)
                                          gATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCAccc                                                       <  1:2631370/101‑1 (MQ=255)
                                          gATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCAccc                                                       >  2:132984/1‑101 (MQ=255)
                                           atcatcAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCAcccc                                                      >  2:3102429/1‑101 (MQ=255)
                                           atcatcAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCAcccc                                                      >  2:1949606/1‑101 (MQ=255)
                                            tcatcaGCAGATCCGCCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCg                                                     <  1:2318071/101‑1 (MQ=255)
                                            tcatcaGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCg                                                     <  1:843719/101‑1 (MQ=255)
                                             catcaGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCgg                                                    >  2:1175016/1‑101 (MQ=255)
                                                 agcagATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGcgcg                                                >  1:2922033/1‑101 (MQ=255)
                                                      aTCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGc                                           >  1:2467869/1‑101 (MQ=255)
                                                      aTCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGc                                           <  1:3417795/101‑1 (MQ=255)
                                                        ccccccccTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGccc                                         <  1:1949735/98‑1 (MQ=255)
                                                         cACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGcccc                                        >  2:715036/1‑101 (MQ=255)
                                                         cACCCCCGCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGcccc                                        <  2:1006404/101‑1 (MQ=255)
                                                          ccccccTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCa                                       <  2:1101014/100‑1 (MQ=255)
                                                          ccccccTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCa                                       <  1:1374616/100‑1 (MQ=255)
                                                          ccccccTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCa                                       <  2:3224450/100‑1 (MQ=255)
                                                          aCCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCa                                       <  1:296610/101‑1 (MQ=255)
                                                           cccccTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAg                                      >  1:3661631/1‑101 (MQ=255)
                                                           cccccTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAg                                      <  1:4239029/101‑1 (MQ=255)
                                                           cccccTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAg                                      <  1:580709/101‑1 (MQ=255)
                                                           cccccTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAg                                      <  2:1151243/101‑1 (MQ=255)
                                                              ccTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGAcc                                   <  1:1168951/101‑1 (MQ=255)
                                                              ccTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGAcc                                   >  2:4206554/1‑101 (MQ=255)
                                                               cTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCt                                  <  1:2251466/101‑1 (MQ=255)
                                                                tCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCtt                                 >  1:3171804/1‑101 (MQ=255)
                                                                 ccgccccccccCGCGTCCGGGCCGCATAGGCGGGCGAGGCCGGGGCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTc                                <  2:2428934/99‑1 (MQ=255)
                                                                 cAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTc                                >  2:1593910/1‑101 (MQ=255)
                                                                 cAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTc                                <  1:360382/101‑1 (MQ=255)
                                                                  aGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCa                               <  1:796782/101‑1 (MQ=255)
                                                                    ccgccgccCGCGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAgc                             <  1:1022744/101‑1 (MQ=255)
                                                                       ccgcccgcGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAgctgc                          <  2:1401882/101‑1 (MQ=255)
                                                                        cgcccgcGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAgctgct                         <  2:4042861/101‑1 (MQ=255)
                                                                         gcccgcGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTc                        <  1:2241742/101‑1 (MQ=255)
                                                                         gcccgcGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTc                        <  1:2194989/101‑1 (MQ=255)
                                                                          cccgcGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCg                       <  1:4174077/101‑1 (MQ=255)
                                                                          cccgcGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCg                       <  1:2001006/101‑1 (MQ=255)
                                                                           ccgcGTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGa                      <  2:3170775/101‑1 (MQ=255)
                                                                            cgcgTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGAc                     <  2:2362624/101‑1 (MQ=255)
                                                                             gcgTCAGCGCGTCATAGGCGAGCTAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGAcc                    <  1:1718327/101‑1 (MQ=255)
                                                                             gcgTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGAcc                    <  1:133005/101‑1 (MQ=255)
                                                                             gcgTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGAcc                    >  2:1901578/1‑101 (MQ=255)
                                                                              cggcAGCGCGTCATAGGCGAGGGAGGCCGGGGCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGAccg                   <  2:1359246/98‑1 (MQ=255)
                                                                              cgTCAGCGCGGCATAGGCGAGCGAGGCGGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGAccg                   <  2:694455/101‑1 (MQ=255)
                                                                               gTCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGAccgc                  <  1:459291/101‑1 (MQ=255)
                                                                                tCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGAccgcc                 >  1:1151855/1‑101 (MQ=255)
                                                                                tCAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCGCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGAccgcg                 <  2:615333/101‑2 (MQ=255)
                                                                                 cAGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGAccgccg                >  1:2871641/1‑101 (MQ=255)
                                                                                  aGCGCGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGACcgccgc               >  2:2675367/1‑101 (MQ=255)
                                                                                   gcgcGTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGACCGCcgcg              <  2:285098/101‑1 (MQ=255)
                                                                                     gcgTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGACCGCcgcggc            <  1:4053713/101‑1 (MQ=255)
                                                                                      cgTCATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGACCGCcgcggcg           >  1:2717776/1‑101 (MQ=255)
                                                                                         cATAGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGACCGCcgcggcgcgg        <  2:1656261/101‑1 (MQ=255)
                                                                                            aGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGACCGCCGCGGCGCGGAAc     >  2:1749129/1‑101 (MQ=255)
                                                                                            aGGCGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGACCGCCGCGGCGCGGAAc     <  2:2312894/101‑1 (MQ=255)
                                                                                               cGAGCGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGACCGCCGCGGCGCGGAACgtg  >  1:726161/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CTCCATCAGATCGGCGCGGTTCTGCAGCCGCCCGGCAGTGTCGATCATCAGCAGATCCACCCCCTCAGCCGCCGCCCGCGTCAGCGCGTCATAGGCGAACGAGGCCGGGTCCGCCCCCTCGGGCGCGGTCATCACCGGCACCCCGGCGCGGTCGCCCCAGACCTTCAGCTGCTCGACCGCCGCGGCGCGGAACGTG  >  NC_014034/2560306‑2560501

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG