Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,566,604 G→A 2.3% intergenic (+76/+118) purD → / ← sdaA phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,566,6040G→A 2.3% 3.0 0.0 ‑0.4170intergenic (+76/+118)purD/sdaAphosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (84/82):  total (86/84)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.11e-01

Only 100 of 184 total aligned reads displayed.

CATCGGCTGGCGCGCGCTCTGAGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCA  >  NC_014034/2566507‑2566704
                                                                                                      |                                                                                                     
cATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAgg                                                                                                       <  1:2985799/101‑1 (MQ=21)
   cGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCt                                                                                                    <  2:1414892/101‑1 (MQ=21)
    ggCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtt                                                                                                   <  1:3569336/101‑1 (MQ=21)
    ggCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtt                                                                                                   <  2:1394035/101‑1 (MQ=21)
    ggCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtt                                                                                                   >  2:1299253/1‑101 (MQ=21)
      cTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                                                                                                 <  2:2134379/101‑1 (MQ=21)
      cTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                                                                                                 <  1:4081669/101‑1 (MQ=21)
      cTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                                                                                                 <  2:327401/101‑1 (MQ=21)
       tGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCGCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                                                                                                <  1:2647695/101‑1 (MQ=21)
       tGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                                                                                                >  1:3459804/1‑101 (MQ=21)
         gcgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCt                                                                                              <  1:4349531/101‑1 (MQ=21)
          cgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                             <  2:3926459/101‑1 (MQ=21)
           gcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                                                                            <  1:2359893/101‑1 (MQ=21)
           gcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                                                                            <  1:3001361/101‑1 (MQ=21)
            cgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                                                                           <  1:1807017/101‑1 (MQ=21)
            cgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTc                                                                                           >  2:413218/1‑101 (MQ=21)
              cgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCCGGGAAATTTCGGGCAAAAAAAAAAAGGTTTTTTCTttttt                                                                                          >  1:3164572/1‑99 (MQ=12)
              cgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                                                                                         <  1:3596700/101‑1 (MQ=21)
               gcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                                                                        <  1:817442/101‑1 (MQ=21)
                  ctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                                                                                     >  2:2689914/1‑101 (MQ=21)
                  ctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCAAATTCG.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                                                                                     <  1:4265530/101‑1 (MQ=21)
                   tGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGAAATTTCCTGCAAGATAAAGAAGGTCTTTTTCTTTCATCTTGc                                                                                    >  1:495608/1‑101 (MQ=18)
                     agaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccc                                                                                  <  2:4443182/101‑1 (MQ=21)
                      gaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                                                                                 <  2:2243119/101‑1 (MQ=21)
                      gaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                                                                                 >  1:1777787/1‑101 (MQ=21)
                        cGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaa                                                                               <  1:740473/101‑1 (MQ=21)
                         gAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTTGGGGAAGATAAAGAAGGGCTTTTCCTTTTATCTTTCCCCaaa                                                                              >  1:3883112/1‑101 (MQ=17)
                          aaacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCT.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAat                                                                             <  1:4394270/96‑1 (MQ=21)
                           aacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAata                                                                            <  1:2176547/97‑1 (MQ=25)
                           aacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAata                                                                            >  1:2794101/5‑101 (MQ=25)
                            acggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat                                                                           >  2:4193579/4‑101 (MQ=25)
                               gggggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCg.ggggg                                                                 <  1:3971574/101‑2 (MQ=0)
                               gggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                                                                        <  1:3095488/101‑1 (MQ=25)
                               gggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                                                                        <  1:2729715/101‑1 (MQ=25)
                                ggggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCg.ggggga                                                                <  2:2846502/101‑3 (MQ=2)
                                 gggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCg.g                                                                     <  1:3108485/101‑1 (MQ=37)
                                   gcgcTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCg.ggg                                                                   <  1:550421/101‑1 (MQ=37)
                                     gcTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCGTTTCATCTTGCCCGAAATATCCCG.GGGGa                                                                 <  2:1102050/101‑1 (MQ=37)
                                     gcTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGa                                                                 <  1:4128436/101‑1 (MQ=37)
                                       tGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGAAATTTCGGGGCAGATAAAGAGGGTTTTTTCCTTTCTTCTTTCCCGGAATATCCCC.CGGGacc                                                               >  1:192838/1‑99 (MQ=16)
                                       tGCCCCC.CTCTTGGCCTTCGGCCAACTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAAGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGc                                                               <  2:2564462/101‑1 (MQ=37)
                                        gCCCCC.CTCTTGGCCTTCGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGcc                                                              <  1:693658/101‑1 (MQ=38)
                                         ccccc.cTCTGG..CTTC.GCC.ATTCA.CCCCCCGGGATATTTCGGGCACGATGA..AAGG.CATCCGGTTTCATCTTGCTGCAAATATCCCGAGGGGAGGCCGATTgc                                                       >  2:2946351/1‑101 (MQ=18)
                                               ctcttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTg                                                        <  1:281610/93‑1 (MQ=255)
                                                tcttggcctccGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTgc                                                       <  2:1499170/91‑1 (MQ=38)
                                                 cttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTgcg                                                      >  1:2922025/7‑101 (MQ=255)
                                                 cttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTgcg                                                      <  1:3958514/95‑1 (MQ=255)
                                                   tggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTgcggg                                                    >  1:2972992/5‑101 (MQ=255)
                                                       cTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGcgggcggg                                                >  2:2325259/1‑101 (MQ=255)
                                                          cGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGc                                             <  1:329854/101‑1 (MQ=255)
                                                          cGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGc                                             >  2:1456184/1‑101 (MQ=255)
                                                           ggCCAATTCG.CCCCCCGGGATATTTCGGGCGAGATAAAGAAGGTCTGTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCa                                            <  2:3689381/101‑1 (MQ=255)
                                                             ccAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGGggggggggc                                          >  1:1153559/1‑97 (MQ=255)
                                                              cAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAgcg                                         <  1:3361897/101‑1 (MQ=255)
                                                               aaTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAgcgc                                        >  1:1584280/1‑101 (MQ=255)
                                                                aTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                       >  2:1839637/1‑101 (MQ=255)
                                                                 ttCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGccc                                      >  1:3644170/1‑101 (MQ=255)
                                                                  tCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGcccc                                     >  2:3359653/1‑101 (MQ=255)
                                                                   cA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                    <  2:2317730/101‑1 (MQ=255)
                                                                   cA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                    <  2:2392515/101‑1 (MQ=255)
                                                                    cgccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                    <  1:1738269/99‑1 (MQ=255)
                                                                     ccccctcCGGATATTTCGGGCAGGATAAAGCAGGTCTTTTCCCTCCACGTTGTCGCAAACACCCCG.GGGGGGCCCGCCAGCGGGCGGGGGCAGCGCCCCCg                                   <  2:1427563/100‑1 (MQ=255)
                                                                     cccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCg                                   <  2:1374118/100‑1 (MQ=255)
                                                                           cGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgcgc                             >  2:4149359/1‑101 (MQ=255)
                                                                            gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                            <  2:4105696/101‑1 (MQ=255)
                                                                              gATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGTACCCCGCTTTCGGGCGGGGGCAGCGCCCCCGAAGTGCCCg                          >  2:1263553/1‑101 (MQ=38)
                                                                              gATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                          >  2:3288299/1‑101 (MQ=255)
                                                                                tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATACCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                        <  2:3980756/101‑1 (MQ=255)
                                                                                 atTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcg                       >  2:2809734/1‑101 (MQ=255)
                                                                                  tttCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgc                      <  2:1164977/101‑1 (MQ=255)
                                                                                     cGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcgcg                   >  1:78253/1‑101 (MQ=255)
                                                                                     cGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcgcg                   <  1:3331345/101‑1 (MQ=255)
                                                                                     cGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcgcg                   >  1:1274712/1‑101 (MQ=255)
                                                                                      gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg                  >  1:1191112/1‑101 (MQ=255)
                                                                                       ggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc                 <  2:3161282/101‑1 (MQ=255)
                                                                                       ggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc                 >  1:1614818/1‑101 (MQ=255)
                                                                                        gCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggcg                <  2:3201781/101‑1 (MQ=255)
                                                                                         cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg               >  2:3375116/1‑101 (MQ=255)
                                                                                         cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg               >  2:853590/1‑101 (MQ=255)
                                                                                         cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg               >  2:2515150/1‑101 (MQ=255)
                                                                                         cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg               >  1:2476696/1‑101 (MQ=255)
                                                                                          naGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgnnnngnn              >  1:612353/2‑99 (MQ=255)
                                                                                          aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg              >  1:1964895/1‑101 (MQ=255)
                                                                                          aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg              <  2:3966646/101‑1 (MQ=255)
                                                                                            gATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCTCCCGGCGCGCGGCGGGCt            <  2:401558/101‑1 (MQ=255)
                                                                                            gATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCt            >  1:2120009/1‑101 (MQ=255)
                                                                                            gATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCt            >  2:107087/1‑101 (MQ=255)
                                                                                             aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt           <  2:1852133/101‑1 (MQ=255)
                                                                                             aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt           <  1:996566/101‑1 (MQ=255)
                                                                                              tAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtcg          >  1:1054335/1‑99 (MQ=255)
                                                                                               aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc         >  2:1166172/1‑101 (MQ=255)
                                                                                               aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc         >  2:55519/1‑101 (MQ=255)
                                                                                                aagaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGGTTgcc        >  1:2823153/1‑101 (MQ=255)
                                                                                                aagaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc        >  1:1602135/1‑101 (MQ=255)
                                                                                                 agaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgccc       <  2:1693902/101‑1 (MQ=255)
                                                                                                  gaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccg      >  1:86799/1‑101 (MQ=255)
                                                                                                  gaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccg      <  1:114711/101‑1 (MQ=255)
                                                                                                  gaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccg      >  2:195873/1‑101 (MQ=255)
                                                                                                    agGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTCgcccgcc    >  2:3849416/1‑101 (MQ=255)
                                                                                                     ggTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgccc   <  1:1417379/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
CATCGGCTGGCGCGCGCTCTGAGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCA  >  NC_014034/2566507‑2566704

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG