Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,566,607 T→A 3.0% intergenic (+79/+115) purD → / ← sdaA phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,566,6070T→A 3.0% 5.8 0.0 ‑0.0167intergenic (+79/+115)purD/sdaAphosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (86/76):  total (89/78)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.03e-01

Only 100 of 190 total aligned reads displayed.

CGGCTGGCGCGCGCTCTGAGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGG  >  NC_014034/2566510‑2566707
                                                                                                      |                                                                                                     
cGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCt                                                                                                       <  2:1414892/101‑1 (MQ=21)
 ggCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtt                                                                                                      <  1:3569336/101‑1 (MQ=21)
 gacTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtt                                                                                                      >  1:3966902/3‑101 (MQ=21)
  gCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCttt                                                                                                     >  1:4154432/1‑101 (MQ=21)
   cTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                                                                                                    <  2:327401/101‑1 (MQ=21)
    tGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                                                                                                   >  1:3459804/1‑101 (MQ=21)
       cgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                                <  1:2822833/101‑1 (MQ=21)
       cgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                                <  2:3926459/101‑1 (MQ=21)
        gcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                                                                               <  1:3001361/101‑1 (MQ=21)
          gcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCa                                                                                             <  2:124700/101‑1 (MQ=21)
           cgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCCGGGAAATTTCGGGCAAAAAAAAAAAGGTTTTTTCTttttt                                                                                             >  1:3164572/1‑99 (MQ=12)
           cgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                                                                                            <  1:3596700/101‑1 (MQ=21)
              tctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCtt                                                                                         >  1:1427670/1‑101 (MQ=21)
               ctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                                                                                        >  2:2689914/1‑101 (MQ=21)
                tGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGAAATTTCCTGCAAGATAAAGAAGGTCTTTTTCTTTCATCTTGc                                                                                       >  1:495608/1‑101 (MQ=18)
                   gaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                                                                                    >  1:1777787/1‑101 (MQ=21)
                   gaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                                                                                    <  1:1785610/101‑1 (MQ=21)
                     cGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaa                                                                                  <  1:740473/101‑1 (MQ=21)
                      gAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTTGGGGAAGATAAAGAAGGGCTTTTCCTTTTATCTTTCCCCaaa                                                                                 >  1:3883112/1‑101 (MQ=17)
                      gAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                                                                                 <  2:4135017/101‑1 (MQ=21)
                      gAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGAAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                                                                                 >  2:1377123/1‑101 (MQ=21)
                       aaacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCT.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAat                                                                                <  1:4394270/96‑1 (MQ=21)
                        aacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAata                                                                               >  1:2794101/5‑101 (MQ=25)
                            gggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                                                                           <  1:2729715/101‑1 (MQ=25)
                            gggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                                                                           <  1:3095488/101‑1 (MQ=25)
                             ggggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCg.ggggga                                                                   <  2:2846502/101‑3 (MQ=2)
                              gggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCg.g                                                                        <  1:3108485/101‑1 (MQ=37)
                                gcgcTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCg.ggg                                                                      <  1:550421/101‑1 (MQ=37)
                                  gcTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCGTTTCATCTTGCCCGAAATATCCCG.GGGGa                                                                    <  2:1102050/101‑1 (MQ=37)
                                  gcTGCCCCC.CTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGa                                                                    <  1:4128436/101‑1 (MQ=37)
                                    tGCCCCC.CTCTTGGCCTTCGGCCAACTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAAGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGc                                                                  <  2:2564462/101‑1 (MQ=37)
                                     gCCCCC.CTCTTGGCCTTCGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGcc                                                                 <  1:693658/101‑1 (MQ=38)
                                      ccccc.cTCTGG..CTTC.GCC.ATTCA.CCCCCCGGGATATTTCGGGCACGATGA..AAGG.CATCCGGTTTCATCTTGCTGCAAATATCCCGAGGGGAGGCCGATTgc                                                          >  2:2946351/1‑101 (MQ=18)
                                          ccctcttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCt                                                             >  1:3981012/11‑101 (MQ=255)
                                           cctcttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGCAATATCCCG.GGGGAGCCCGCtt                                                            >  2:4177887/10‑101 (MQ=37)
                                            ctcttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTg                                                           <  1:281610/93‑1 (MQ=255)
                                              cttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTgcg                                                         >  1:2922025/7‑101 (MQ=255)
                                                tggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTgcggg                                                       >  1:2972992/5‑101 (MQ=255)
                                                 ggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCCTgccggc                                                      >  2:2330775/4‑101 (MQ=255)
                                                       cGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGc                                                <  1:329854/101‑1 (MQ=255)
                                                          ccAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGGggggggggc                                             >  1:1153559/1‑97 (MQ=255)
                                                           cAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAgcg                                            <  1:3361897/101‑1 (MQ=255)
                                                             aTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                          >  2:1839637/1‑101 (MQ=255)
                                                              ttCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGccc                                         >  1:3644170/1‑101 (MQ=255)
                                                               tCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGcccc                                        <  2:2266890/101‑1 (MQ=255)
                                                               tCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGcccc                                        >  2:3359653/1‑101 (MQ=255)
                                                                cA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                       >  2:1404180/1‑101 (MQ=255)
                                                                cA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                       <  1:1890989/101‑1 (MQ=255)
                                                                 cgccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                       <  1:1738269/99‑1 (MQ=255)
                                                                    cccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGaa                                    <  2:2318409/101‑1 (MQ=255)
                                                                      cccGGGATATTTCGGGCTAGATAAAGATGGTCTTTTCCTTTCATCTTGCCCAAAATATCCCG.GGGGTTCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgc                                  <  2:3970547/101‑1 (MQ=38)
                                                                         gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                               <  2:4105696/101‑1 (MQ=255)
                                                                           gATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                             >  2:3288299/1‑101 (MQ=255)
                                                                             tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                           >  2:1474047/1‑101 (MQ=255)
                                                                             tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATACCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                           <  2:3980756/101‑1 (MQ=255)
                                                                              atTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcg                          >  2:2809734/1‑101 (MQ=255)
                                                                               tttCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgc                         <  2:1164977/101‑1 (MQ=255)
                                                                                ttCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcg                        >  1:428516/1‑101 (MQ=255)
                                                                                ttCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcg                        >  1:2178200/1‑101 (MQ=255)
                                                                                  cGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcgcg                      >  1:78253/1‑101 (MQ=255)
                                                                                  cGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcgcg                      >  1:1274712/1‑101 (MQ=255)
                                                                                  cGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcgcg                      <  1:3331345/101‑1 (MQ=255)
                                                                                   gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGGGCCCGACGCgcgg                     >  2:4425910/1‑101 (MQ=255)
                                                                                   gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg                     >  2:1340149/1‑101 (MQ=255)
                                                                                   gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg                     >  1:1191112/1‑101 (MQ=255)
                                                                                    ggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc                    <  2:3161282/101‑1 (MQ=255)
                                                                                    ggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc                    >  1:1614818/1‑101 (MQ=255)
                                                                                     gCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggcg                   <  2:3201781/101‑1 (MQ=255)
                                                                                      cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg                  >  2:3375116/1‑101 (MQ=255)
                                                                                      cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg                  >  1:2476696/1‑101 (MQ=255)
                                                                                      cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg                  >  2:853590/1‑101 (MQ=255)
                                                                                       naGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgnnnngnn                 >  1:612353/2‑99 (MQ=255)
                                                                                       aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                 >  1:1964895/1‑101 (MQ=255)
                                                                                       aaGATAAAGAAGGTCTTTTCCTTTCATCTTGACCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGAGGCggg                 >  1:2532798/1‑101 (MQ=255)
                                                                                         gATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCTCCCGGCGCGCGGCGGGCt               <  2:401558/101‑1 (MQ=255)
                                                                                         gATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCt               >  2:107087/1‑101 (MQ=255)
                                                                                         gATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCt               >  1:2120009/1‑101 (MQ=255)
                                                                                          aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt              <  2:1852133/101‑1 (MQ=255)
                                                                                          aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt              <  1:996566/101‑1 (MQ=255)
                                                                                           tAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtcg             >  1:1054335/1‑99 (MQ=255)
                                                                                            aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc            >  2:55519/1‑101 (MQ=255)
                                                                                             aagaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc           >  1:1602135/1‑101 (MQ=255)
                                                                                             aagaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc           >  2:1433106/1‑101 (MQ=255)
                                                                                              agaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgccc          <  2:1693902/101‑1 (MQ=255)
                                                                                              agaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCCTgccc          >  2:1264771/1‑101 (MQ=255)
                                                                                               gaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccg         <  1:114711/101‑1 (MQ=255)
                                                                                               gaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccg         >  1:86799/1‑101 (MQ=255)
                                                                                                aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgc        >  2:2225098/1‑101 (MQ=255)
                                                                                                aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgc        >  2:2418701/1‑101 (MQ=255)
                                                                                                 agGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTCgcccgcc       >  2:3849416/1‑101 (MQ=255)
                                                                                                   gTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCa     >  1:823562/1‑101 (MQ=255)
                                                                                                   gTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCa     >  2:2010502/1‑101 (MQ=255)
                                                                                                    tCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCaa    >  2:2313715/1‑101 (MQ=255)
                                                                                                     cTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag   >  2:1510093/1‑101 (MQ=255)
                                                                                                     cTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag   >  1:2858775/1‑101 (MQ=255)
                                                                                                      ttttCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCCagg  >  2:3960001/1‑101 (MQ=255)
                                                                                                      ttttCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg  >  1:408609/1‑101 (MQ=255)
                                                                                                      ttttCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg  <  2:533973/101‑1 (MQ=255)
                                                                                                      ttttCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGCGGGCAGCGCCCCCGAACCGCCCGACGCGCGGCGGGCTTGCCCCCCCAagg  >  1:130823/1‑101 (MQ=255)
                                                                                                      ttttCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGAGGGGGCAGCGCCCCCGAAGCGCCCGACCGGCGGCGGGCTTGCCCGACCAagg  >  2:3625028/1‑101 (MQ=38)
                                                                                                      |                                                                                                     
CGGCTGGCGCGCGCTCTGAGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGG  >  NC_014034/2566510‑2566707

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG