Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,566,610 T→C 3.0% intergenic (+82/+112) purD → / ← sdaA phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,566,6100T→C 3.0% 3.9 0.0 ‑0.1167intergenic (+82/+112)purD/sdaAphosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (86/75):  total (89/78)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.48e-01

Only 100 of 193 total aligned reads displayed.

CTGGCGCGCGCTCTGAGACGAAAC.GGGGGCGCTGCCCCCCTCCTG..CTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGG  >  NC_014034/2566513‑2566710
                                                                                                     |                                                                                                    
cTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                                                                                                      <  2:327401/101‑1 (MQ=21)
cTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                                                                                                      <  1:885005/101‑1 (MQ=21)
 tGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCGCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                                                                                                     <  1:2647695/101‑1 (MQ=21)
    cgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                                  <  1:2822833/101‑1 (MQ=21)
    cgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                                  <  2:1094175/101‑1 (MQ=21)
     gcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                                                                                 <  1:3001361/101‑1 (MQ=21)
     gcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                                                                                 <  1:2359893/101‑1 (MQ=21)
       gcgcTCTGAGACGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCa                                                                                               <  2:89802/101‑1 (MQ=21)
        cgcTCTGAGACGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCCGGGAAATTTCGGGCAAAAAAAAAAAGGTTTTTTCTttttt                                                                                               >  1:3164572/1‑99 (MQ=12)
        cgcTCTGAGACGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                                                                                              <  1:3596700/101‑1 (MQ=21)
         gcTCTGAGACGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                                                                             <  1:817442/101‑1 (MQ=21)
            ctGAGACGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                                                                                          >  1:1274440/1‑101 (MQ=21)
             tGAGACGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGAAATTTCCTGCAAGATAAAGAAGGTCTTTTTCTTTCATCTTGc                                                                                         >  1:495608/1‑101 (MQ=18)
              gagaCGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGcc                                                                                        <  2:271613/101‑1 (MQ=21)
                gaCGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                                                                                      <  1:1785610/101‑1 (MQ=21)
                  cGAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaa                                                                                    <  1:740473/101‑1 (MQ=21)
                   gAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTTGGGGAAGATAAAGAAGGGCTTTTCCTTTTATCTTTCCCCaaa                                                                                   >  1:3883112/1‑101 (MQ=17)
                   gAAACGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                                                                                   <  2:4135017/101‑1 (MQ=21)
                    aaacggggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCT.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAat                                                                                  <  1:4394270/96‑1 (MQ=21)
                     aacggggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAata                                                                                 <  1:2176547/97‑1 (MQ=25)
                     aacggggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAata                                                                                 <  1:3403555/97‑1 (MQ=25)
                      acggggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat                                                                                <  1:1158219/98‑1 (MQ=25)
                        agggggCGCTG.CCCCCTCTTGGCCTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg                                                                        >  1:2760966/2‑101 (MQ=0)
                        agggggCGCTG.CCCCCTCTTGGCCTTTGGCCAATTCA..CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg                                                                        >  1:3465033/2‑101 (MQ=0)
                         gggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                                                                             <  1:2802997/101‑1 (MQ=25)
                         gggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                                                                             <  1:3095488/101‑1 (MQ=25)
                           gggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCgg                                                                           <  1:3108485/101‑1 (MQ=37)
                             gcgcTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCgggg                                                                         <  1:550421/101‑1 (MQ=37)
                               gcTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGa                                                                       <  1:4128436/101‑1 (MQ=37)
                                cTGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATACTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAg                                                                      >  2:3937016/1‑101 (MQ=37)
                                 tGCCCCCCTCTTGGCCTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGc                                                                     >  2:1008137/1‑101 (MQ=37)
                                  gCCCCCCTCTTGGCCTTCGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGcc                                                                    <  1:693658/101‑1 (MQ=38)
                                      ccctcttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCt                                                                >  1:3981012/11‑101 (MQ=255)
                                       cctcttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGCAATATCCCGGGGGAGCCCGCtt                                                               >  2:4177887/10‑101 (MQ=37)
                                       cctcttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCtt                                                               <  2:2849051/92‑1 (MQ=255)
                                          cttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcg                                                            <  1:3958514/95‑1 (MQ=255)
                                          cttggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcg                                                            >  1:2922025/7‑101 (MQ=255)
                                            tggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcggg                                                          >  1:2972992/5‑101 (MQ=255)
                                             ggccTTCGGCCAATTCC.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgggc                                                         <  1:3289530/98‑1 (MQ=255)
                                             ggccTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCCTgccggc                                                         >  2:2330775/4‑101 (MQ=255)
                                                cTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGcgggcggg                                                      >  2:2325259/1‑101 (MQ=255)
                                                   cGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                                   >  2:1158868/1‑101 (MQ=255)
                                                    ggCCAATTCG.CCCCCCGGGATATTTCGGGCGAGATAAAGAAGGTCTGTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCa                                                  <  2:3689381/101‑1 (MQ=255)
                                                    ggCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCa                                                  <  1:1299350/101‑1 (MQ=255)
                                                       cAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAgcg                                               <  1:3361897/101‑1 (MQ=255)
                                                        aaTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAgcgc                                              >  1:1584280/1‑101 (MQ=255)
                                                         aTTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                             >  2:1839637/1‑101 (MQ=255)
                                                          ttCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccc                                            >  1:3644170/1‑101 (MQ=255)
                                                           tCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcccc                                           >  1:1776744/1‑101 (MQ=255)
                                                           tCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcccc                                           <  2:2266890/101‑1 (MQ=255)
                                                           tCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcccc                                           >  2:3359653/1‑101 (MQ=255)
                                                            cA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                          <  1:1890989/101‑1 (MQ=255)
                                                            cA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                          >  2:1404180/1‑101 (MQ=255)
                                                            cA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                                          <  2:2317730/101‑1 (MQ=255)
                                                            cA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTTCTTTCATCTTGCCCGAAATATCCCGGGGGGGCCCGCTTGCGGGGGGGGGGCGCGccccc                                          >  2:3072127/1‑101 (MQ=21)
                                                              cccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCg                                         <  2:1374118/100‑1 (MQ=255)
                                                                cccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGaa                                       >  2:2669688/1‑101 (MQ=255)
                                                                    cGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgcgc                                   >  2:3884936/1‑101 (MQ=255)
                                                                     gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                                  <  2:4105696/101‑1 (MQ=255)
                                                                       gATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGTACCCCGCTTTCGGGCGGGGGCAGCGCCCCCGAAGTGCCCg                                >  2:1263553/1‑101 (MQ=38)
                                                                       gATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                                >  2:3288299/1‑101 (MQ=255)
                                                                         tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                              >  2:2838482/1‑101 (MQ=255)
                                                                         tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                              >  2:1474047/1‑101 (MQ=255)
                                                                         tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATACCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                              <  2:3980756/101‑1 (MQ=255)
                                                                            ttCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcg                           >  1:428516/1‑101 (MQ=255)
                                                                               gggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGGGCCCGACGCgcgg                        >  2:4425910/1‑101 (MQ=255)
                                                                                ggCAAAATAAACAAGGTCTTTTCCTTTCATCTTTCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc                       <  1:3299003/101‑1 (MQ=255)
                                                                                 gCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggcg                      >  1:1149322/1‑101 (MQ=255)
                                                                                 gCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggcg                      <  2:3201781/101‑1 (MQ=255)
                                                                                  cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg                     >  2:3375116/1‑101 (MQ=255)
                                                                                  cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg                     >  2:2515150/1‑101 (MQ=255)
                                                                                   naGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgnnnngnn                    >  1:612353/2‑99 (MQ=255)
                                                                                   aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                    <  2:3966646/101‑1 (MQ=255)
                                                                                   aaGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg                    >  1:1964895/1‑101 (MQ=255)
                                                                                     gATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCTCCCGGCGCGCGGCGGGCt                  <  2:401558/101‑1 (MQ=255)
                                                                                     gATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCt                  >  1:2120009/1‑101 (MQ=255)
                                                                                      aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt                 <  2:1852133/101‑1 (MQ=255)
                                                                                       tAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtcg                >  1:1054335/1‑99 (MQ=255)
                                                                                        aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGGTTgc               >  1:160862/1‑101 (MQ=255)
                                                                                        aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc               >  2:55519/1‑101 (MQ=255)
                                                                                        aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc               >  1:1732047/1‑101 (MQ=255)
                                                                                        aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc               >  2:1166172/1‑101 (MQ=255)
                                                                                         aagaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc              >  2:1433106/1‑101 (MQ=255)
                                                                                          agaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgccc             <  2:1693902/101‑1 (MQ=255)
                                                                                          agaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCCTgccc             >  2:1264771/1‑101 (MQ=255)
                                                                                            aagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgc           >  2:2225098/1‑101 (MQ=255)
                                                                                              ggTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcccgccc         <  1:1417379/101‑1 (MQ=255)
                                                                                               gTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCa        >  2:2010502/1‑101 (MQ=255)
                                                                                                tCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCaa       >  1:2518637/1‑101 (MQ=255)
                                                                                                 cTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag      >  2:1510093/1‑101 (MQ=255)
                                                                                                 cTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag      >  1:2858775/1‑101 (MQ=255)
                                                                                                  ttttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg     >  1:408609/1‑101 (MQ=255)
                                                                                                  ttttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg     <  2:533973/101‑1 (MQ=255)
                                                                                                  ttttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGAGGGGGCAGCGCCCCCGAAGCGCCCGACCGGCGGCGGGCTTGCCCGACCAagg     >  2:3625028/1‑101 (MQ=38)
                                                                                                   tttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga    >  1:1082312/1‑101 (MQ=255)
                                                                                                   tttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga    >  2:838764/1‑101 (MQ=255)
                                                                                                    ttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag   >  2:2409043/1‑101 (MQ=255)
                                                                                                    ttCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag   >  1:4359464/1‑101 (MQ=255)
                                                                                                     tCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCNACGCGCGGCGGGCTTGCCCGCCCAaggagg  >  1:847922/1‑101 (MQ=255)
                                                                                                     tCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggagg  >  1:771253/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
CTGGCGCGCGCTCTGAGACGAAAC.GGGGGCGCTGCCCCCCTCCTG..CTTCGGCCAATTCA.CCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGG  >  NC_014034/2566513‑2566710

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG