Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 257,090 C→G 100% intergenic (+24/‑175) secA → / → RCAP_rcc00221 preprotein translocase subunit SecA/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034257,0900C→G100.0% 605.2 161intergenic (+24/‑175)secA/RCAP_rcc00221preprotein translocase subunit SecA/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (87/74):  ref base (0/0):  total (87/74)

Only 100 of 176 total aligned reads displayed.

GAGCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGCCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCGG  >  NC_014034/256992‑257187
                                                                                                  |                                                                                                    
  gCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCggg                                                                                                  >  1:527571/1‑100 (MQ=255)
    ccTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTcc                                                                                                <  2:2461055/101‑1 (MQ=255)
     cTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCg                                                                                               <  2:2587764/101‑1 (MQ=255)
      tCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCgg                                                                                              <  2:4324537/101‑1 (MQ=255)
       cgcgCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCggg                                                                                             <  2:1055503/101‑1 (MQ=255)
         cgcAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGCCggggg                                                                                           >  1:3217091/1‑100 (MQ=255)
          gcAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGCCgggggg                                                                                          >  2:4075042/1‑99 (MQ=255)
           cAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGGGGGCCggggggg                                                                                         >  1:3332016/1‑98 (MQ=255)
           cAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGGGGGCCGGGGG.Ga                                                                                        >  1:2404564/1‑101 (MQ=255)
               gccccctgcccctgCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAAccc                                                                                    <  2:2796413/99‑1 (MQ=255)
               gACCCCTGCCCCTGGGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAAccc                                                                                    <  1:3400784/101‑1 (MQ=255)
               gACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAAccc                                                                                    <  2:4241893/101‑1 (MQ=255)
               gACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAAccc                                                                                    <  1:811642/101‑1 (MQ=255)
                aCCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCg                                                                                   <  2:2350209/101‑1 (MQ=255)
                 cccctgcccctgCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCgg                                                                                  <  2:796764/101‑1 (MQ=255)
                  ccctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCggg                                                                                 <  2:2105554/101‑1 (MQ=255)
                    ctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGGGGGCCGGGGG.GGAcccccggc                                                                               >  1:100361/1‑96 (MQ=255)
                    ctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGgcc                                                                               <  1:3170560/101‑1 (MQ=255)
                    ctgcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGgcc                                                                               <  2:2707501/101‑1 (MQ=255)
                      gcccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGCCGGGGG.GGAACCCGgccgc                                                                             >  1:1735780/1‑101 (MQ=255)
                       cccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGgccgcc                                                                            <  2:2148734/101‑1 (MQ=255)
                        ccctgcGGCTCGGGCGAGAAGTTCAAGCCCTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGccgccg                                                                           <  2:1458381/101‑1 (MQ=255)
                        ccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGccgccg                                                                           <  2:1252661/101‑1 (MQ=255)
                        ccctgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGCCGGGGG.GGACCCCGGcccccg                                                                           >  1:1908926/1‑101 (MQ=255)
                           tgcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGt.tt                                                                       <  2:4282795/101‑1 (MQ=255)
                            gcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGGCGGGGG.GAAACCCGGGCGCCGc.ttt                                                                      >  1:425691/1‑101 (MQ=255)
                            gcGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCGGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGt.ttt                                                                      <  1:4376814/101‑1 (MQ=255)
                             cGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGt.tttt                                                                     <  1:4011133/101‑1 (MQ=255)
                             cGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGCCGGGGG.GGACCCCGGGCGCCGc.tttt                                                                     >  1:4448312/1‑101 (MQ=255)
                              ggCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGCAATATGGCGGTGCTCCAGGTTGGAACGCGGgccgcccctttc                                                                      >  2:1760927/1‑95 (MQ=255)
                              ggCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGCCGGGGG.GGACCCCGGCCGCCGc.ttttt                                                                    >  2:1510579/1‑100 (MQ=255)
                               gCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCa                                                                   <  2:527519/101‑1 (MQ=255)
                                cTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCAt                                                                  <  2:3756556/101‑1 (MQ=255)
                                  cGGGCGAGAAGTTCAAGCACTGCCACGGCCTTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTa                                                                <  1:304921/101‑1 (MQ=255)
                                      cGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGGGGGTCGCGGG.GCACCCGGGCCGCCGT.TTTTCATTTTCCt                                                            >  2:1410840/1‑101 (MQ=255)
                                      cGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCt                                                            <  2:3234940/101‑1 (MQ=255)
                                       gagaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGCCGGGGG.GAACCCGGGCCGCCGG.TTTTTCTTTTCCtt                                                           >  1:3041055/1‑100 (MQ=255)
                                        agaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGGGGGCCGGGGT.CTACCCCGGTTCCCGC.TTTTTTTTCTCCTgc                                                          >  2:2420485/1‑100 (MQ=255)
                                        agaAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGG.TTTTTCTTATCCttt                                                          >  1:1817187/1‑99 (MQ=255)
                                        agaAGTGCAACCACTGCCGCGGCCTTGTGGCCTGAAACAAGGGCGCTGAAATATGGCGGGGGTCCGGGGA.GAACCCGGGCCGCCGT.TTTTCATTATCCTGt                                                          <  1:2420642/101‑1 (MQ=255)
                                          aaGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGCCGGGGG.GGACCCGGGCCGCCGT.TTTTTCTTTTCCTGTTg                                                        >  1:1794945/1‑101 (MQ=255)
                                           aGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGT.TTTTTTTTTTCCTGtttt                                                       >  1:3236320/1‑99 (MQ=255)
                                            gTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGAt                                                      <  1:1502987/101‑1 (MQ=255)
                                              tCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGGGGGTCGGGGG.GGAGCGCGGCCGCCGG.TTTTCATTTTCCTGTTggttc                                                    >  2:3544741/1‑97 (MQ=255)
                                              tCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCt                                                    <  1:3688489/101‑1 (MQ=255)
                                               cAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCCGGCCGCCGG.TTTTCATTATCCTGTTGTTCTg                                                   >  1:1122130/1‑101 (MQ=255)
                                                aaGCACTGCCACGGCCGTATGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTgg                                                  <  1:3954043/101‑1 (MQ=255)
                                                 aGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGa                                                 <  2:3867451/101‑1 (MQ=255)
                                                  gtaCTGCCACGGCCGTCTGGCCTAAATCAAAGGCGCTAAAACATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGat                                                <  2:4417741/99‑1 (MQ=255)
                                                  gCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGat                                                <  1:2302892/101‑1 (MQ=255)
                                                  gCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGat                                                <  1:2484212/101‑1 (MQ=255)
                                                     cTGCCAGGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.Tg                                            <  1:649141/101‑1 (MQ=255)
                                                        ccACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGttt                                         <  2:4318709/101‑1 (MQ=255)
                                                         cACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGtttt                                        <  1:997911/101‑1 (MQ=255)
                                                         cACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAAACCGGGCCGCCGT.GTTTCATTATCCTTTTGATCTGGATA.TGtttt                                        >  1:3759867/1‑101 (MQ=255)
                                                          aCGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGCCGGGGG.GAACCCGGGGCGCCGC.TTTTCATTATCCTGTTTATCTGGATA.TGttttt                                       >  1:3624514/1‑101 (MQ=255)
                                                           cGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTtg                                      <  1:3722708/101‑1 (MQ=255)
                                                            ggCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTtgt                                     <  2:1284179/101‑1 (MQ=255)
                                                            ggCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTtgt                                     <  2:4448040/101‑1 (MQ=255)
                                                            ggCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGttttttt                                     >  1:1665353/1‑99 (MQ=255)
                                                            ggCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCCGGGG.GGAACCGGGCCGCCGC.TTTTTATTATCCTGTTGATCTGGGTA.TGttttttt                                     >  1:3113589/1‑99 (MQ=255)
                                                            ggCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGCCGGGGG.GGACCCGGGCCGCCGC.TTTTTATTTTTCTGTTTATCTGGGTA.TGttttttt                                     >  2:3009188/1‑99 (MQ=255)
                                                               cGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGCGCGCGGT.GGACCCGGGCCGCCGG.TTTTCATTATCCCTCTTATCTTGGAA.TGTTTTTTTGGa                                  >  2:1958192/1‑101 (MQ=255)
                                                                gTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGT.TTTTTATTTTCCTGTTTATCTGGATA.TGTTTTTGTggga                                 >  2:823012/1‑99 (MQ=255)
                                                                gTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTTTGGaa                                 >  1:2240295/1‑101 (MQ=255)
                                                                gTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGGAGGACCGGGGG.GCACGCGGGCGCCCGTGTTTTCTTTTTCTTG.TGGTGCGGGTA.TGTTTTTGTTGaa                                 >  1:1607800/1‑101 (MQ=255)
                                                                  cTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGCCGGGGG.GAACCCGGGCCGCCGT.TTTTTATTATCCTGTTTATCTGGATA.TGTTTTTTTGGAAAt                               >  2:3913723/1‑101 (MQ=255)
                                                                  cTGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGCCGGGGG.GAACCCGGGCCGCCGG.TTTTCATTATTCTGTTTATCTTGATA.TGTTTTTTTGGAAAt                               >  2:3277905/1‑101 (MQ=255)
                                                                   tGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAAtt                              <  2:2451794/101‑1 (MQ=255)
                                                                   tGGCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAAtt                              <  2:2852958/101‑1 (MQ=255)
                                                                    ggCCTGAAACAAAGGCGCTGAAATATGGCGGCGGGCCGGGGG.GAACCCCGGCCGCCGG.TTTTCATTATCCTGTTGATCTGGATATTTTTTTTTTGGAAAtt                              >  2:339226/1‑101 (MQ=255)
                                                                     gCCTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAATTTc                            <  1:1083762/101‑1 (MQ=255)
                                                                      ccTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTTTGGAAAttttc                           >  1:4145492/1‑99 (MQ=255)
                                                                      ccTGAAACAAAGGCGCTGAAATATGGCGGCGGGCCGGGGG.GAACCCGGGGCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGGAATTTcc                           >  2:1567950/1‑101 (MQ=255)
                                                                      ccTGAAACAAAGGCGCTGAAATATGGCGGCGGGCCGGGGG.GAACCCGGGCCGCCGG.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAAttttc                           >  2:3612195/1‑99 (MQ=255)
                                                                       cTGAAACAAAGGCGCTGAAATATGGCGGGGGTCCGGGGG.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTTTGGAAATTTtcg                          >  2:3660455/1‑101 (MQ=255)
                                                                       cTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAATTTccg                          <  2:982278/101‑1 (MQ=255)
                                                                       cTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAATTTccg                          <  2:1106574/101‑1 (MQ=255)
                                                                       cTGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGCTCTGGGTA.TGTTTTTTTGGGAATTTtcg                          >  1:440113/1‑101 (MQ=255)
                                                                        tGAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAATTTccgg                         <  1:2072199/101‑1 (MQ=255)
                                                                        tGAAACAAAGGCGCTGAAATATGGCGGCGGGCCGGGGG.GAACCCCGGCCGCCGC.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTTTGGAAATTTccgg                         >  1:1010225/1‑101 (MQ=255)
                                                                         gAAACAAAGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTTTGGAAATTTccggc                        >  2:3555431/1‑101 (MQ=255)
                                                                          aaaCAAAGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGT.TTTTTATTATTCTGTTGCTCTGGGTA.TGTTTTTTTGGAAATTTtcggcc                       >  1:1315621/1‑101 (MQ=255)
                                                                          aaaCAAAGGCGCTGAAATATGGCGGCCGTCCGGGGG.GGACCCGGGCCGCCGC.TTTTCATTATCCCTTTGATCTTGATA.TTTTTTTTTTGAAATTTccggcc                       >  1:2953760/1‑101 (MQ=255)
                                                                           aaCAAAGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGTTCTGGGTA.TGTTTTTTTGGAAATTTccggccg                      >  1:2157681/1‑101 (MQ=255)
                                                                            aCAAAGGCGCTGAAATATGGCGGCGGGCCGGGGG.GGAACCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAATTTtcggccgg                     >  2:1296275/1‑101 (MQ=255)
                                                                             cAAAGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGT.TTTTTATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAATTTCCGGCCggg                    >  2:2170723/1‑101 (MQ=255)
                                                                             cAAAGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGG.TTTTCATTATCCTGTTTTTCTGGGTA.TGTTTTTTTGGGAATTTTCGGCCggg                    >  1:2785247/1‑101 (MQ=255)
                                                                              aaaGGCGCTGAAATATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAATTTCCGGCCGGGc                   <  2:1331101/101‑1 (MQ=255)
                                                                              aaaGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCCGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAATTTTCCGCCGGGc                   >  2:2926704/1‑101 (MQ=255)
                                                                              aaaGGCGCTGAAATATGGCGGCGGGCCGGGGG.GAACCCGGGCCGCCGG.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTTTGGAAATTTCCGGCCCGGc                   >  1:2680637/1‑101 (MQ=255)
                                                                               aaGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTTTGGAAATTTCCGGCCGGGCg                  >  1:2344550/1‑101 (MQ=255)
                                                                               aaGGCGCTGAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTTTGGAAATTTCCGGCCGGGCg                  >  1:1460650/1‑101 (MQ=255)
                                                                                       gAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGT.TTTTTATTATCCTGTTGATCTGGATA.TGTTTTTTTGGAAATTTCCGGCCGGGCGAAGGTTTc          >  1:2598255/1‑101 (MQ=255)
                                                                                       gAAATATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGG.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGGTTc          >  2:3779215/1‑101 (MQ=255)
                                                                                        aaaTATGGCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTcc         <  2:2877840/101‑1 (MQ=255)
                                                                                        aaaTATGGCGGCGGTCCGGGGG.GAACCCGGGCCGCCGT.TTTTTATTATCCTGTTGATCTGGATA.TGTTTTTTTGGAAATTTCCGGCCGGGCGAAGGGTTcc         >  2:1973745/1‑101 (MQ=255)
                                                                                        aaaTATGGCGGCGCTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTcc         <  2:4385461/101‑1 (MQ=255)
                                                                                              ggCGGCGGTCGGCGCT.GCACCCGGCCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCg   <  2:3969824/101‑1 (MQ=255)
                                                                                              ggCGGCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCg   >  2:3493052/1‑101 (MQ=255)
                                                                                                  |                                                                                                    
GAGCCCTCGCGCAATGACCCCTGCCCCTGCGGCTCGGGCGAGAAGTTCAAGCACTGCCACGGCCGTCTGGCCTGAAACAAAGGCGCTGAAATATGGCGCCGGTCCGGGGT.GAACCCGGGCCGCCGT.TTTTCATTATCCTGTTGATCTGGATA.TGTTTTTGTGGAAATTTCCGGCCGGGCGAAGGTTTCCGTTTCGG  >  NC_014034/256992‑257187

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG