Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,590,068 C→G 100% V12L (GTT→CTT)  glnE ← glutamate‑ammonia‑ligase adenylyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,590,0680C→G100.0% 584.0 153V12L (GTT→CTT) glnEglutamate‑ammonia‑ligase adenylyltransferase
Reads supporting (aligned to +/- strand):  new base (60/93):  ref base (0/0):  total (60/93)

Only 100 of 160 total aligned reads displayed.

CGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAACGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTT  >  NC_014034/2589970‑2590165
                                                                                                  |                                                                                                 
ccgacggCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGgg                                                                                                 <  1:4388834/95‑1 (MQ=255)
cGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGgg                                                                                                 <  2:2366588/101‑1 (MQ=255)
cGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGgg                                                                                                 >  2:510888/1‑101 (MQ=255)
 gAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGggg                                                                                                >  1:3089413/1‑101 (MQ=255)
  aaCAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGc                                                                                               <  1:3448801/101‑1 (MQ=255)
  aaCAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGc                                                                                               <  2:3738145/101‑1 (MQ=255)
  aaCAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGc                                                                                               <  1:4364635/101‑1 (MQ=255)
    cAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGt                                                                                             <  2:1029792/101‑1 (MQ=255)
    aaGCCCGCCGTGCCCCCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGt                                                                                             <  1:1987588/100‑1 (MQ=255)
     aGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTg                                                                                            >  1:2493639/1‑101 (MQ=255)
      gCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGc                                                                                           >  1:4189166/1‑101 (MQ=255)
      gCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGc                                                                                           <  1:4344516/101‑1 (MQ=255)
         cgccgTGCCCGCGATCAGCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTTCggg                                                                                        <  2:1683046/101‑1 (MQ=255)
         cgccgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCggg                                                                                        <  2:8279/101‑1 (MQ=255)
         cgccgGGCCCGCGGTCAACCCCCCGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGAggg                                                                                        <  2:920827/101‑1 (MQ=255)
            cgTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCa                                                                                     >  2:2387011/1‑101 (MQ=255)
             gTGCCCGCGATCACCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAg                                                                                    <  2:1864513/101‑1 (MQ=255)
             gTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAg                                                                                    >  1:1692744/1‑101 (MQ=255)
              tGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAgg                                                                                   <  1:3076123/101‑1 (MQ=255)
                cccGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCg                                                                                 <  2:285279/101‑1 (MQ=255)
                   gcgATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGa                                                                              <  1:2294734/101‑1 (MQ=255)
                    cgATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGAt                                                                             <  1:1392946/101‑1 (MQ=255)
                     gagcagcccccGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATg                                                                            <  2:655200/95‑1 (MQ=255)
                     gATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCATGCGGGagg                                                                            >  1:2672319/1‑99 (MQ=255)
                      accAACCCCCGGATTTCGGGCGGAAAATCGACATAACGGGCAACGACATCGGCGCCGCGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGc                                                                           <  1:2982324/99‑1 (MQ=255)
                      aTCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGc                                                                           >  2:2789223/1‑101 (MQ=255)
                       tCAACCCCCGGATTTAGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGNGGANGGGGCGTGCGGGTCAGGAGGGATGcg                                                                          >  1:3618197/1‑100 (MQ=255)
                         aaCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAg                                                                        >  2:2580183/1‑101 (MQ=255)
                         aaCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAg                                                                        <  2:15659/101‑1 (MQ=255)
                         aaCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAg                                                                        >  2:2102615/1‑101 (MQ=255)
                         aaCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAg                                                                        >  2:1943700/1‑101 (MQ=255)
                          aCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAgg                                                                       <  1:1759431/101‑1 (MQ=255)
                          aCCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAgg                                                                       >  1:1157342/1‑101 (MQ=255)
                           cccccGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGc                                                                      <  1:886452/101‑1 (MQ=255)
                           cccccGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGc                                                                      <  1:4086793/101‑1 (MQ=255)
                            ccccGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCt                                                                     <  2:3571324/101‑1 (MQ=255)
                            ccccGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCt                                                                     <  1:105744/101‑1 (MQ=255)
                             cccGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATNGGCGCCACGGGCCGGATCAAGGGGAAGNNNNNNGCGGNTNAGGNNGNATGCAAGGCTc                                                                    >  1:3637725/1‑101 (MQ=255)
                             cccGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTc                                                                    <  1:1258285/101‑1 (MQ=255)
                             cccGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTc                                                                    <  1:2692088/101‑1 (MQ=255)
                             cccGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTc                                                                    >  1:2148279/1‑101 (MQ=255)
                               cGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCAt                                                                  <  2:3926496/101‑1 (MQ=255)
                                ggATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATg                                                                 <  1:3434188/101‑1 (MQ=255)
                                  aTTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGgc                                                               <  1:1202753/101‑1 (MQ=255)
                                    ttCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGgcgc                                                             >  2:3507180/1‑101 (MQ=255)
                                     tCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGcc                                                            <  2:1678442/101‑1 (MQ=255)
                                     tCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGcc                                                            <  1:788116/101‑1 (MQ=255)
                                      cGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCa                                                           >  2:3679018/1‑101 (MQ=255)
                                        ggCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGa                                                         >  2:1947311/1‑101 (MQ=255)
                                         gCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGAc                                                        <  2:1157251/101‑1 (MQ=255)
                                          cGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACt                                                       <  1:2668148/101‑1 (MQ=255)
                                           ggAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTa                                                      >  1:108600/1‑101 (MQ=255)
                                           ggAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTa                                                      >  1:2194647/1‑101 (MQ=255)
                                            gAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAg                                                     >  1:2111527/1‑101 (MQ=255)
                                              aaaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgcc                                                   >  2:1367359/1‑101 (MQ=255)
                                               aaTCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAgccg                                                  <  1:2191524/101‑1 (MQ=255)
                                                    ccATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCa                                             <  2:3481100/101‑1 (MQ=255)
                                                    ccATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCa                                             <  2:3914955/101‑1 (MQ=255)
                                                     cATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCaa                                            <  1:1801108/101‑1 (MQ=255)
                                                     cATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCaa                                            >  1:3278129/1‑101 (MQ=255)
                                                        aaCGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGcc                                         >  2:3855390/1‑101 (MQ=255)
                                                        aaCGGGCAACGACATCGGCGCCACGGGCAGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGcc                                         >  2:3308091/1‑101 (MQ=255)
                                                         aCGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGccc                                        <  1:540246/101‑1 (MQ=255)
                                                           gggCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCg                                      <  1:1301017/101‑1 (MQ=255)
                                                            ggCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCgg                                     <  2:1890612/101‑1 (MQ=255)
                                                            ggCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCgg                                     <  1:3519581/101‑1 (MQ=255)
                                                             gCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCggg                                    <  2:1727865/101‑1 (MQ=255)
                                                               aaggACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTCCGGGTCAGGCGGGATGCAAGGCCCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTc                                  <  1:4218385/98‑1 (MQ=255)
                                                               aaCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTc                                  >  2:1438164/1‑101 (MQ=255)
                                                                aCGACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCa                                 <  2:1265040/101‑1 (MQ=255)
                                                                  gACATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAg                               >  2:4435434/1‑101 (MQ=255)
                                                                    cATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgc                             <  2:2148140/101‑1 (MQ=255)
                                                                    cATCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgc                             <  1:3531659/101‑1 (MQ=255)
                                                                     aTCGGCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgcg                            <  1:2275008/101‑1 (MQ=255)
                                                                        ggCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgcgcgc                         >  2:4113272/1‑101 (MQ=255)
                                                                        ggCGCCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGgcgcgc                         >  1:647122/1‑101 (MQ=255)
                                                                           gcCACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCaaa                      >  1:133926/1‑101 (MQ=255)
                                                                            ccACGGGCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAAt                     <  2:3277897/101‑1 (MQ=255)
                                                                                 ggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcccc                <  2:828031/101‑1 (MQ=255)
                                                                                 ggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcccc                >  2:1324096/1‑101 (MQ=255)
                                                                                 ggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcccc                <  1:4426414/101‑1 (MQ=255)
                                                                                 ggCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgcccc                <  1:129867/101‑1 (MQ=255)
                                                                                  gCCGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccg               >  1:2397538/1‑101 (MQ=255)
                                                                                    cGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgcc             <  2:4188905/101‑1 (MQ=255)
                                                                                    cGGATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgcc             >  2:1380983/1‑101 (MQ=255)
                                                                                     ggATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgccc            >  1:2217862/1‑101 (MQ=255)
                                                                                     ggATCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgccc            >  2:4388957/1‑101 (MQ=255)
                                                                                      gagcAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATgccccgcccc           <  1:2833777/98‑1 (MQ=255)
                                                                                       agaagggggAGGGGGTGTGCGGGTCAGGGGGGATCCTAGGCTCATGGCGCCATACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCt          <  2:3617944/96‑1 (MQ=255)
                                                                                        tCAAGGGGAAGGGGGGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTg         <  2:108579/101‑1 (MQ=255)
                                                                                        tCAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTg         <  1:140781/101‑1 (MQ=255)
                                                                                         cAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGt        <  2:1521423/101‑1 (MQ=255)
                                                                                         cAAGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGt        >  2:4237767/1‑101 (MQ=255)
                                                                                          aaGGGGAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTc       >  2:727983/1‑101 (MQ=255)
                                                                                            ggggAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCAt     >  1:4141945/1‑101 (MQ=255)
                                                                                            ggggAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCAt     <  1:556843/101‑1 (MQ=255)
                                                                                              ggAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCt   <  1:2580354/101‑1 (MQ=255)
                                                                                              ggAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCACGCCCCTGTCATCt   <  1:166589/101‑1 (MQ=255)
                                                                                              ggAAGGGGCGTGCGGGTCAGGCGGGATCCAAGGCTCATGGCGCGAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCt   <  2:2193738/101‑1 (MQ=255)
                                                                                               gAAGGGGCGTGCGGGTCAGGCGGGATGCAAGGCCCATGGCGCCGTACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCtt  <  2:3499507/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CGAACAGCCCGCCGTGCCCGCGATCAACCCCCGGATTTCGGGCGGAAAATCGCCATAACGGGCAACGACATCGGCGCCACGGGCCGGATCAAGGGGAACGGGCGTGCGGGTCAGGCGGGATGCAAGGCTCATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTT  >  NC_014034/2589970‑2590165

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG