Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,603,050	A→C	100%	E160A (GAG→GCG)	RCAP_rcc02427 →	M23 family peptidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,603,050	0	A→C	100.0%	98.2	26	E160A (GAG→GCG)	RCAP_rcc02427	M23 family peptidase
Reads supporting (aligned to +/- strand):  new base (17/9):  ref base (0/0):  total (17/9)

CGCGGGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCGCCGAGCC  >  NC_014034/2602952‑2603146                                                                                                   |                                                                                                   cgcgGGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCgcga                                                                                                  <  1:3387038/101‑1 (MQ=255)     ggcgggGCGGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACG.cc                                                                                             <  1:1976720/101‑1 (MQ=255)      ggggggCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACG.ccc                                                                                            <  2:880459/99‑1 (MQ=255)         gggCTGCCGCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACG.CCcgcg                                                                                         <  1:2287981/101‑1 (MQ=255)           gCTGCCGCCGGGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACG.CCCGCGG.c                                                                                      <  2:1741380/101‑1 (MQ=255)                    gcgGGCAGCGAGCGGATCGACATCCCCGCGCGGGCGGGGGCCGCGATCGCCCGCGCCGCCGCCCCCCAGCCCCCCGCCGCGACG.CCCGCGG.CCCCCGCCAc                                                                             <  1:772115/101‑1 (MQ=255)                      gggCAGGGAGCGGACCGACATCACCTCGCTGGCGGGGGCCGCGAGCGCCCGCGCCGCCGTCCCCCAGCCGCCCGCCGCGACG.CCCGCGG.CCCCCGCCACcg                                                                           <  1:871494/101‑1 (MQ=255)                           gcgagcgGATCGACATCACCTCGCTGGCGGGGGGCGCGGGCGGCCGCGCCGCCGCCCCCCCCCCGCCCCCCCCGGCG.CCCCCCG.CCCCCCCCCCCCCCccc                                                                      >  1:238784/1‑101 (MQ=255)                             gagcgGATCGACATCACCTCGCTGGCGGGGGCCGGGGAAGAACCCCCCGCCCGCACCCAACCCCCCAGCGCGGCG.GCCGCCG.CCCCCCCCCCCCCGccccc                                                                    >  2:684940/1‑100 (MQ=255)                             atctggATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGCCCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCg                                                                    <  1:3628928/97‑1 (MQ=255)                                 ggATCGACATCACCTCGCTGGCGGGGGGGGCGATGGAGCGCGCCGCCCCCCCCCCCCCCCCCGCCCCGCCGCCCCCCGGCCCCCCCCCCCCCCGCCCCgag                                                                  >  2:3628675/1‑99 (MQ=255)                                          tccccTCGCTGGCGGGGCCCGCAATCCCCCGCGCCCCCGTCCCCCAGCCGCCCGCCGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCa                                                       <  1:685020/98‑1 (MQ=255)                                                     gggggggCCGGGAGCGCCCGCCCCGCCGTCGCCAACCCCCCCGCCGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGccc                                            <  1:659120/99‑1 (MQ=255)                                                       gggggCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGCCCCGCCGCGCCAAGTGCCAGCCCgg                                          >  1:3270681/1‑101 (MQ=255)                                                             cgcgATCGCCCGCCCCGCCGTCCCCCAGCCCCCCGCCGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGa                                    <  2:238757/101‑1 (MQ=255)                                                              cgtcgggcccGCGCCTCCGCCCCCAGGCCGCCCGCGCCGCCG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGaa                                   <  2:2527084/93‑1 (MQ=255)                                                               cgATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGaaa                                  >  2:3842892/1‑101 (MQ=255)                                                                          gccgccGTCACCCAGCCGCCCGCCGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgcccc                       >  2:3607296/1‑101 (MQ=255)                                                                                 tCACCCAGCCGCCCGCCGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgcccccgccaga                >  2:2206550/1‑98 (MQ=255)                                                                                  cACCCAGCCGCCCGCCGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGa               >  2:3827139/1‑101 (MQ=255)                                                                                   aCCCAGCCGCCCGCCGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGAc              >  2:2117966/1‑101 (MQ=255)                                                                                       aGCCGCCCGCCGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGgc          >  2:4441587/1‑101 (MQ=255)                                                                                       aGCCGCCCGCCGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGgc          >  1:1278449/1‑101 (MQ=255)                                                                                       aGCCGCCCGCCGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGgc          >  1:2634291/1‑101 (MQ=255)                                                                                       aGCCGCCCGCCGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTACCGACGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGgc          >  1:1674270/1‑101 (MQ=255)                                                                                         ccgcccgcCGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGgcgc        >  1:4054213/1‑101 (MQ=255)                                                                                           gcccgccGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCgccg      >  1:2249272/1‑101 (MQ=255)                                                                                           gcccgccGCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCgccg      >  1:3911465/1‑101 (MQ=255)                                                                                             ccgccgCGACG.CCCGCGG.CCCCCGCCACCGCGCGCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCgccgag    <  1:3843145/101‑1 (MQ=255)                                                                                              cgccgCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCgccgagc   >  2:1594379/1‑101 (MQ=255)                                                                                              cgccgCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCgccgagc   >  2:3806767/1‑101 (MQ=255)                                                                                               gccgCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCgccgagcc  >  2:3636028/1‑101 (MQ=255)                                                                                               gccgCGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCgccgagcc  >  1:348941/1‑101 (MQ=255)                                                                                                   |                                                                                                   CGCGGGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACG.CCCGCGG.CCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCGCCGAGCC  >  NC_014034/2602952‑2603146 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG