Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,182 A→G 26.3% intergenic (‑158/+118) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1820A→G26.3% 84.0 ‑1.9 ‑0.0125intergenic (‑158/+118)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/14):  ref base (39/53):  total (58/67)
Fisher's exact test strand distribution p-value = 1.57e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.65e-01

Only 100 of 134 total aligned reads displayed.

GGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTT  >  NC_014034/2640084‑2640283
                                                                                                    |                                                                                                     
ggTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAg                                                                                                      <  2:3215552/101‑1 (MQ=255)
ggTATGCGACGCGGCGGCAGGCGTGTCAACCGTCGCGCTTTTGCGCAGGCAAAAGGGCGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAg                                                                                                      <  1:3809351/101‑1 (MQ=37)
   aTGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTa                                                                                                   <  2:3112156/101‑1 (MQ=255)
      ggACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTAttt                                                                                                <  1:2306650/100‑1 (MQ=255)
      cGACGGGGCTGTAGTCGTGTCAACCGTGGCGCTTTTGCCCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTAttt                                                                                                <  2:3937291/101‑1 (MQ=21)
      cGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTAttt                                                                                                <  1:4444468/101‑1 (MQ=255)
        aCGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTgg                                                                                              <  2:497954/101‑1 (MQ=255)
               ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTAACCCCCCGAGGTATTTGGAGCAAGa                                                                                       >  2:1463435/1‑101 (MQ=25)
                 cAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                                                     <  1:146990/101‑1 (MQ=37)
                    gcggtgccaCCGGGGCCGTTTTGCGCCAGCAAAAGGGGGGCCCCGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                  <  1:2615763/93‑1 (MQ=12)
                         tCAACCGTGGCGCGTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCa                                                                            <  1:155103/101‑1 (MQ=21)
                           aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                          <  1:94532/101‑1 (MQ=25)
                             ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                        <  2:3936702/101‑1 (MQ=25)
                                 ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                                                    <  1:3877557/101‑1 (MQ=25)
                                    gcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCCTCGGCCAATTCACCCCCCCGGGGATTTGGAGGAAGATG.AAAGAACAAGGGTTCATcc                                                                 >  1:1390025/1‑100 (MQ=16)
                                      ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTTGC.CTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATGAAAA.ACCATGGGTTCATCTTg                                                               >  2:4264698/1‑101 (MQ=21)
                                      ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                               <  2:3070734/101‑1 (MQ=21)
                                      ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                               <  2:3821418/101‑1 (MQ=25)
                                          gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                           <  2:2245420/101‑1 (MQ=25)
                                             gccccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                       >  2:381633/7‑101 (MQ=14)
                                              ccccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAata                                                      <  2:1672072/96‑1 (MQ=17)
                                               cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                     <  1:1435728/97‑1 (MQ=17)
                                                ccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                                                    >  2:3812970/4‑101 (MQ=17)
                                                 cgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                   >  1:2516379/3‑101 (MQ=17)
                                                 cAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                    <  1:1662996/101‑1 (MQ=25)
                                                  gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                  >  1:983552/2‑101 (MQ=17)
                                                  gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                  <  2:1214460/100‑1 (MQ=17)
                                                  gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                  <  2:2584643/100‑1 (MQ=17)
                                                  gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                  >  2:2237596/2‑101 (MQ=17)
                                                  gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                  >  1:3221725/2‑101 (MQ=17)
                                                  aaaaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATcc                                                   <  1:1864880/101‑1 (MQ=21)
                                                   aaaGGGGGGCGCTGCCGCCCTC.TTGGCCTTCGGTCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                  <  2:2034580/101‑1 (MQ=25)
                                                   aaaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCCATTCACCCCCCGAGGGATTTGGGGCAAGATG.AAAGACCATAGGGTCATCTTGCCTCAAATATccc                                                  >  2:4382118/1‑101 (MQ=18)
                                                   aaaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACGCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                  <  2:2958783/101‑1 (MQ=25)
                                                   aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                 >  1:3317379/1‑101 (MQ=17)
                                                   aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                 <  2:4434161/101‑1 (MQ=17)
                                                   aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                 >  1:284981/1‑101 (MQ=17)
                                                   aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                 >  1:501348/1‑101 (MQ=17)
                                                    aaagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                >  2:1754790/4‑101 (MQ=17)
                                                    aaagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                >  2:2633283/4‑101 (MQ=17)
                                                    aaagggagCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                <  1:151104/98‑1 (MQ=18)
                                                    aaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCg                                                 <  2:3915414/101‑1 (MQ=25)
                                                     aagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                               >  2:3995351/3‑101 (MQ=18)
                                                       gggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                             <  2:2442551/101‑1 (MQ=18)
                                                        ggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                            <  2:4170680/101‑2 (MQ=18)
                                                         gggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                           >  1:1483656/1‑99 (MQ=18)
                                                         gggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                            <  1:3798836/101‑1 (MQ=25)
                                                         gggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGGTCATCTTGCCTCAAATATCCCgggggg                                            >  1:2066226/1‑101 (MQ=21)
                                                          ggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                          <  2:2365971/101‑4 (MQ=21)
                                                            cgcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                        <  2:3386072/101‑6 (MQ=21)
                                                             gcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                       >  2:2897688/1‑95 (MQ=21)
                                                             gcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGCGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggtgaggc                                       >  1:2673094/1‑95 (MQ=21)
                                                             gcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGGGTc                                        >  1:2493273/1‑101 (MQ=25)
                                                              gctgcccccTC.TTGGCCTTTGGCCAATTC.CCCCCTGGGATATTTCGGGCAAGATGAAATCA.TGCCCCTTCATCTTGCCGCAAATATCCCGGGGGGAgcgaa                                      <  2:519174/97‑5 (MQ=2)
                                                              cTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                      >  1:157205/1‑94 (MQ=25)
                                                              cTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                       >  2:258998/1‑101 (MQ=25)
                                                              cTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                       >  2:1747285/1‑101 (MQ=25)
                                                                gCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggggaggcgaa                                    >  2:60573/1‑93 (MQ=21)
                                                                gCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                     <  1:1819846/101‑1 (MQ=37)
                                                                 ccccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                                    <  2:19556/101‑1 (MQ=37)
                                                                  tctccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                   <  2:3142271/98‑1 (MQ=37)
                                                                  cccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                   >  1:3381854/1‑101 (MQ=37)
                                                                  cccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                   <  2:1107672/101‑1 (MQ=37)
                                                                   ccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                  >  2:331491/1‑101 (MQ=37)
                                                                     ccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                <  1:2747996/101‑1 (MQ=37)
                                                                     ccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                >  2:1598174/1‑101 (MQ=37)
                                                                     ccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                <  1:978469/101‑1 (MQ=37)
                                                                     ccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAga                                >  1:3142495/1‑100 (MQ=37)
                                                                       tccgatctcTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                               <  2:1831815/93‑1 (MQ=25)
                                                                          ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                            >  2:3963879/6‑101 (MQ=37)
                                                                            ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                          >  2:748150/4‑101 (MQ=37)
                                                                            ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGCGGGAGTCCTGAAAGGACgggg                          >  2:3965748/4‑101 (MQ=37)
                                                                             gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                         >  1:208038/3‑101 (MQ=37)
                                                                             gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                         <  1:3135966/99‑1 (MQ=37)
                                                                              ccTTCCGCCAATTCCCCCCCCGAGGTATTTGG.ACAAGGATAAAGGTCGAGGGGTTCACCTTGCTTAAAATATCCGGGGGGGAGTCCTGAAAGGACgggggg                        <  1:2908755/100‑1 (MQ=255)
                                                                               cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGaagatcgga                       >  1:1831939/1‑93 (MQ=37)
                                                                                 tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                     <  2:1389928/101‑1 (MQ=255)
                                                                                   ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                   >  1:1566253/1‑101 (MQ=255)
                                                                                   ggCCAACTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                   <  1:2543800/101‑1 (MQ=37)
                                                                                    gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                  <  2:863949/101‑1 (MQ=255)
                                                                                      cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                >  2:4261646/1‑101 (MQ=37)
                                                                                      cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                >  2:106276/1‑101 (MQ=37)
                                                                                       aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc               <  1:1121558/101‑1 (MQ=255)
                                                                                       aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc               >  2:4430714/1‑101 (MQ=37)
                                                                                        aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCAAGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc              <  1:3478645/101‑1 (MQ=37)
                                                                                         ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc             >  2:200119/1‑101 (MQ=255)
                                                                                         ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc             >  1:4186309/1‑101 (MQ=255)
                                                                                         ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc             >  1:1268778/1‑101 (MQ=255)
                                                                                           caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc           >  2:2603201/3‑101 (MQ=255)
                                                                                           caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCACCCTc           >  2:3365057/3‑101 (MQ=37)
                                                                                            cccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc          <  1:4264967/100‑1 (MQ=255)
                                                                                             ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc         <  2:2476156/101‑1 (MQ=255)
                                                                                              cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg        <  1:2603372/101‑1 (MQ=255)
                                                                                              cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg        <  1:1598280/101‑1 (MQ=255)
                                                                                                cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg      <  1:458166/101‑1 (MQ=255)
                                                                                                 ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt     >  2:3411138/1‑101 (MQ=255)
                                                                                                  cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgggcc    >  1:4288510/1‑99 (MQ=255)
                                                                                                   gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt   <  2:2817531/101‑1 (MQ=255)
                                                                                                   gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt   >  2:3192324/1‑101 (MQ=255)
                                                                                                    aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt  >  2:1614620/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
GGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTT  >  NC_014034/2640084‑2640283

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG