Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,184 G→A 26.1% intergenic (‑160/+116) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1840G→A26.1% 87.3 ‑1.8 0.0126intergenic (‑160/+116)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/14):  ref base (40/53):  total (59/67)
Fisher's exact test strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

ATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTC  >  NC_014034/2640087‑2640285
                                                                                                  |                                                                                                     
   ggACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTAttt                                                                                                  <  1:2306650/100‑1 (MQ=255)
     aCGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTgg                                                                                                <  2:497954/101‑1 (MQ=255)
       gcggcgGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGag                                                                                              <  2:3005194/101‑2 (MQ=255)
        cggcggCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGagc                                                                                             <  2:4286988/101‑3 (MQ=255)
            ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                                                         <  2:70213/101‑1 (MQ=37)
            ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTAACCCCCCGAGGTATTTGGAGCAAGa                                                                                         >  2:1463435/1‑101 (MQ=25)
              gaGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                                                       <  2:2587362/100‑1 (MQ=25)
              cAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                                                       <  1:146990/101‑1 (MQ=37)
                 gcggtgccaCCGGGGCCGTTTTGCGCCAGCAAAAGGGGGGCCCCGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                    <  1:2615763/93‑1 (MQ=12)
                   gtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                                                  <  2:2107149/101‑3 (MQ=25)
                      tCAACCGTGGCGCGTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCa                                                                              <  1:155103/101‑1 (MQ=21)
                          ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                          <  2:3936702/101‑1 (MQ=25)
                              ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                                                      <  1:3877557/101‑1 (MQ=25)
                                 gcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCCTCGGCCAATTCACCCCCCCGGGGATTTGGAGGAAGATG.AAAGAACAAGGGTTCATcc                                                                   >  1:1390025/1‑100 (MQ=16)
                                   ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                 <  2:3070734/101‑1 (MQ=21)
                                          gccccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                         >  2:381633/7‑101 (MQ=14)
                                           ccccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAata                                                        <  2:1672072/96‑1 (MQ=17)
                                             ccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                                                      >  2:3812970/4‑101 (MQ=17)
                                              cgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                     >  1:2516379/3‑101 (MQ=17)
                                              cAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                      <  1:1662996/101‑1 (MQ=25)
                                               gaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                    >  2:2237596/2‑101 (MQ=17)
                                               gaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                    >  1:983552/2‑101 (MQ=17)
                                               gaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                    <  2:1214460/100‑1 (MQ=17)
                                               gaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                    <  2:2584643/100‑1 (MQ=17)
                                               gaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                    >  1:3221725/2‑101 (MQ=17)
                                               aaaaGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATcc                                                     <  1:1864880/101‑1 (MQ=21)
                                                aaaGGGGGGCGCTGCCGCCCTCTTGGCCTTCGGTCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                    <  2:2034580/101‑1 (MQ=25)
                                                aaaGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCCATTCACCCCCCGAGGGATTTGGGGCAAGATG.AAAGACCATAGGGTCATCTTGCCTCAAATATccc                                                    >  2:4382118/1‑101 (MQ=18)
                                                aaaGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACGCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                    <  2:2958783/101‑1 (MQ=25)
                                                aaaGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                    <  2:3542036/101‑1 (MQ=21)
                                                aaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                   >  1:3317379/1‑101 (MQ=17)
                                                aaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                   >  1:284981/1‑101 (MQ=17)
                                                aaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                   >  1:501348/1‑101 (MQ=17)
                                                 aaagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                  >  2:2633283/4‑101 (MQ=17)
                                                 aaGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCg                                                   <  2:3915414/101‑1 (MQ=25)
                                                  aagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                 <  1:3925122/99‑1 (MQ=18)
                                                   ggggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATGAAAA.ACCATGGGGTCATCTTTCCTCAAATATACCggg                                                 >  2:1520875/1‑101 (MQ=18)
                                                    gggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                               >  1:2364239/1‑101 (MQ=18)
                                                    gggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                               <  2:2442551/101‑1 (MQ=18)
                                                     ggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                              <  2:4170680/101‑2 (MQ=18)
                                                      gggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                             >  1:1483656/1‑99 (MQ=18)
                                                      gggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                              <  1:3798836/101‑1 (MQ=25)
                                                      gggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATACCgggggg                                              >  2:3952654/1‑101 (MQ=21)
                                                       ggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                            <  2:2365971/101‑4 (MQ=21)
                                                       ggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                            <  1:2684185/101‑4 (MQ=21)
                                                       ggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                             <  1:1569019/101‑1 (MQ=25)
                                                        gcgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                           <  2:1301882/101‑5 (MQ=21)
                                                        gcgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGACATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                           >  2:170973/1‑97 (MQ=21)
                                                        gcgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTTTTTGGAGCAAGATG.AAAGAACATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                            >  1:1491290/1‑101 (MQ=25)
                                                         cgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                          <  2:3386072/101‑6 (MQ=21)
                                                          gcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGCGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggtgaggc                                         >  1:2673094/1‑95 (MQ=21)
                                                           gctgcccccTCTTGGCCTTTGGCCAATTC.CCCCCTGGGATATTTCGGGCAAGATGAAATCA.TGCCCCTTCATCTTGCCGCAAATATCCCGGGGGGAgcgaa                                        <  2:519174/97‑5 (MQ=2)
                                                           cTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                         >  2:258998/1‑101 (MQ=25)
                                                           cTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                         >  2:1747285/1‑101 (MQ=25)
                                                              ccccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                                      <  2:19556/101‑1 (MQ=37)
                                                               tctccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                     <  2:3142271/98‑1 (MQ=37)
                                                               cccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                     >  1:3381854/1‑101 (MQ=37)
                                                               cccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                     <  2:1107672/101‑1 (MQ=37)
                                                                  ccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                  >  2:192453/1‑101 (MQ=37)
                                                                  ccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                  >  2:1598174/1‑101 (MQ=37)
                                                                  ccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                  <  1:978469/101‑1 (MQ=37)
                                                                  ccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAga                                  >  1:3142495/1‑100 (MQ=37)
                                                                   tccgatctcTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                 <  2:1831815/93‑1 (MQ=25)
                                                                    tctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                >  2:458126/1‑101 (MQ=37)
                                                                      ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                              >  2:3963879/6‑101 (MQ=37)
                                                                      ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                              >  1:196128/6‑101 (MQ=25)
                                                                       tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                             >  2:2729220/5‑101 (MQ=37)
                                                                        ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                            >  2:748150/4‑101 (MQ=37)
                                                                        ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGCGGGAGTCCTGAAAGGACgggg                            >  2:3965748/4‑101 (MQ=37)
                                                                         gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                           >  1:208038/3‑101 (MQ=37)
                                                                         gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                           <  1:3135966/99‑1 (MQ=37)
                                                                          ccTTCCGCCAATTCCCCCCCCGAGGTATTTGG.ACAAGGATAAAGGTCGAGGGGTTCACCTTGCTTAAAATATCCGGGGGGGAGTCCTGAAAGGACgggggg                          <  1:2908755/100‑1 (MQ=255)
                                                                           cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGaagatcgga                         >  1:1831939/1‑93 (MQ=37)
                                                                           cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                         >  1:1412095/1‑101 (MQ=37)
                                                                               ggCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                     <  2:3832394/101‑1 (MQ=255)
                                                                               ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                     >  1:4394021/1‑101 (MQ=37)
                                                                               ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                     >  1:1566253/1‑101 (MQ=255)
                                                                               ggCCAACTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                     <  1:2543800/101‑1 (MQ=37)
                                                                                gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                    <  2:863949/101‑1 (MQ=255)
                                                                                  cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAANGACGGGGGGCAGAGccc                  >  2:1005714/1‑101 (MQ=37)
                                                                                  cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                  >  2:4261646/1‑101 (MQ=37)
                                                                                   aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                 <  1:1121558/101‑1 (MQ=255)
                                                                                   aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                 >  2:4430714/1‑101 (MQ=37)
                                                                                    aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCAAGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                <  1:3478645/101‑1 (MQ=37)
                                                                                     ttCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc               <  1:830262/101‑1 (MQ=255)
                                                                                     ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc               >  1:1268778/1‑101 (MQ=255)
                                                                                      tcaccccccGAGGTATTTGGCGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt              >  2:335974/4‑101 (MQ=25)
                                                                                       caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc             <  2:4048920/99‑1 (MQ=255)
                                                                                       caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCACCCTc             >  2:3365057/3‑101 (MQ=37)
                                                                                        cccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc            <  1:4264967/100‑1 (MQ=255)
                                                                                         ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc           <  1:260154/101‑1 (MQ=255)
                                                                                         ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc           >  1:1780964/1‑101 (MQ=255)
                                                                                          cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg          <  1:1598280/101‑1 (MQ=255)
                                                                                            cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg        <  1:458166/101‑1 (MQ=255)
                                                                                             ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt       >  2:315494/1‑101 (MQ=255)
                                                                                              cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgggcc      >  1:4288510/1‑99 (MQ=255)
                                                                                               gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt     <  2:2817531/101‑1 (MQ=255)
                                                                                               gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt     >  2:3192324/1‑101 (MQ=255)
                                                                                                aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt    <  2:2390866/101‑1 (MQ=255)
                                                                                                  gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc  >  1:2914141/1‑101 (MQ=255)
                                                                                                  |                                                                                                     
ATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTC  >  NC_014034/2640087‑2640285

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG