Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,192 A→G 27.1% intergenic (‑168/+108) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1920A→G27.1% 88.9 ‑0.6 ‑0.1127intergenic (‑168/+108)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/15):  ref base (44/48):  total (64/63)
Fisher's exact test strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.50e-01

Only 100 of 136 total aligned reads displayed.

GCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640094‑2640291
                                                                                                   |                                                                                                   
gcggcgGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGag                                                                                                    <  2:3005194/101‑2 (MQ=255)
 cggcggCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGagc                                                                                                   <  2:4286988/101‑3 (MQ=255)
     ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTAACCCCCCGAGGTATTTGGAGCAAGa                                                                                               >  2:1463435/1‑101 (MQ=25)
         ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                           <  1:3280783/101‑1 (MQ=25)
          gcggtgccaCCGGGGCCGTTTTGCGCCAGCAAAAGGGGGGCCCCGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                          <  1:2615763/93‑1 (MQ=12)
            gtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                                                        <  2:2107149/101‑3 (MQ=25)
                   ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                                <  2:3936702/101‑1 (MQ=25)
                       ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                                                            <  1:3877557/101‑1 (MQ=25)
                            ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                       <  2:3821418/101‑1 (MQ=25)
                            ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                       <  2:3070734/101‑1 (MQ=21)
                                gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                   <  2:2245420/101‑1 (MQ=25)
                                   gccccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                               >  2:381633/7‑101 (MQ=14)
                                    ccccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAata                                                              <  2:1672072/96‑1 (MQ=17)
                                     cccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                             <  1:1435728/97‑1 (MQ=17)
                                      ccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                                                            >  2:3812970/4‑101 (MQ=17)
                                       cAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                                                            <  1:1662996/101‑1 (MQ=25)
                                        gaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                          <  2:1214460/100‑1 (MQ=17)
                                        gaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                          >  1:983552/2‑101 (MQ=17)
                                        gaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                          <  2:2584643/100‑1 (MQ=17)
                                        aaaaGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATcc                                                           <  1:1864880/101‑1 (MQ=21)
                                         aaaGGGGGGCGCTGCCGCCCTCTTGGCCTTCGGTCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                          <  2:2034580/101‑1 (MQ=25)
                                         aaaGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCCATTCACCCCCCGAGGGATTTGGGGCAAGATG.AAAGACCATAGGGTCATCTTGCCTCAAATATccc                                                          >  2:4382118/1‑101 (MQ=18)
                                         aaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                         >  1:284981/1‑101 (MQ=17)
                                         aaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                         >  1:3317379/1‑101 (MQ=17)
                                          aaagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                        >  2:2633283/4‑101 (MQ=17)
                                          aaagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                        >  2:1754790/4‑101 (MQ=17)
                                          aaagggagCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                        <  1:151104/98‑1 (MQ=18)
                                          aaGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCg                                                         <  2:3915414/101‑1 (MQ=25)
                                            ggggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                       <  1:3064119/101‑1 (MQ=25)
                                            ggggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATGAAAA.ACCATGGGGTCATCTTTCCTCAAATATACCggg                                                       >  2:1520875/1‑101 (MQ=18)
                                             gggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                     <  2:2442551/101‑1 (MQ=18)
                                             gggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                     >  1:2364239/1‑101 (MQ=18)
                                              ggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                                    <  2:4170680/101‑2 (MQ=18)
                                               gggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                   >  1:1483656/1‑99 (MQ=18)
                                               gggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                    <  1:3798836/101‑1 (MQ=25)
                                                ggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                  <  2:2365971/101‑4 (MQ=21)
                                                ggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                   <  1:1569019/101‑1 (MQ=25)
                                                 gcgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                 <  1:371556/101‑5 (MQ=21)
                                                 gcgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGACATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                 >  2:170973/1‑97 (MQ=21)
                                                 gcgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTTTTTGGAGCAAGATG.AAAGAACATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                                  >  1:1491290/1‑101 (MQ=25)
                                                  cgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                <  2:3386072/101‑6 (MQ=21)
                                                   gcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                               >  2:2897688/1‑95 (MQ=21)
                                                   gcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGCGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggtgaggc                                               >  1:2673094/1‑95 (MQ=21)
                                                   gcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGGGTc                                                >  1:2493273/1‑101 (MQ=25)
                                                    gctgcccccTCTTGGCCTTTGGCCAATTC.CCCCCTGGGATATTTCGGGCAAGATGAAATCA.TGCCCCTTCATCTTGCCGCAAATATCCCGGGGGGAgcgaa                                              <  2:519174/97‑5 (MQ=2)
                                                    cTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                              >  1:157205/1‑94 (MQ=25)
                                                    cTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                               >  2:258998/1‑101 (MQ=25)
                                                      gCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggggaggcgaa                                            >  2:60573/1‑93 (MQ=21)
                                                        cccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                           >  1:3381854/1‑101 (MQ=37)
                                                        cccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                           <  2:1107672/101‑1 (MQ=37)
                                                         ccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                          >  2:331491/1‑101 (MQ=37)
                                                           ccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                        >  2:192453/1‑101 (MQ=37)
                                                           ccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                        <  1:2747996/101‑1 (MQ=37)
                                                           ccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                        <  1:978469/101‑1 (MQ=37)
                                                           ccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAga                                        >  1:3142495/1‑100 (MQ=37)
                                                            tccgatctcTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                       <  2:1831815/93‑1 (MQ=25)
                                                             tctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                      >  2:458126/1‑101 (MQ=37)
                                                               ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                    >  1:196128/6‑101 (MQ=25)
                                                               ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                    >  2:3963879/6‑101 (MQ=37)
                                                                 ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                  >  2:748150/4‑101 (MQ=37)
                                                                 ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGCGGGAGTCCTGAAAGGACgggg                                  >  2:3965748/4‑101 (MQ=37)
                                                                  gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                 >  1:208038/3‑101 (MQ=37)
                                                                  gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                 <  1:3135966/99‑1 (MQ=37)
                                                                   ccTTCCGCCAATTCCCCCCCCGAGGTATTTGG.ACAAGGATAAAGGTCGAGGGGTTCACCTTGCTTAAAATATCCGGGGGGGAGTCCTGAAAGGACgggggg                                <  1:2908755/100‑1 (MQ=255)
                                                                    cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGaagatcgga                               >  1:1831939/1‑93 (MQ=37)
                                                                    cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                               >  1:1412095/1‑101 (MQ=37)
                                                                      tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                             <  2:1389928/101‑1 (MQ=255)
                                                                        ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                           >  1:1566253/1‑101 (MQ=255)
                                                                        ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                           >  1:4394021/1‑101 (MQ=37)
                                                                        ggCCAACTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                           <  1:2543800/101‑1 (MQ=37)
                                                                         gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                          <  2:863949/101‑1 (MQ=255)
                                                                           cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAANGACGGGGGGCAGAGccc                        >  2:1005714/1‑101 (MQ=37)
                                                                           cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                        >  2:4261646/1‑101 (MQ=37)
                                                                            aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                       >  2:4430714/1‑101 (MQ=37)
                                                                            aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                       <  1:1121558/101‑1 (MQ=255)
                                                                              ttCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                     <  1:830262/101‑1 (MQ=255)
                                                                              ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                     >  2:200119/1‑101 (MQ=255)
                                                                              ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                     >  1:4186309/1‑101 (MQ=255)
                                                                              ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                     >  1:1268778/1‑101 (MQ=255)
                                                                                caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   <  2:4048920/99‑1 (MQ=255)
                                                                                caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   >  2:2603201/3‑101 (MQ=255)
                                                                                caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCACCCTc                   >  2:3365057/3‑101 (MQ=37)
                                                                                  ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                 <  1:260154/101‑1 (MQ=255)
                                                                                  ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                 >  1:1780964/1‑101 (MQ=255)
                                                                                  ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                 <  2:2476156/101‑1 (MQ=255)
                                                                                     cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg              <  1:458166/101‑1 (MQ=255)
                                                                                      ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt             >  2:3411138/1‑101 (MQ=255)
                                                                                      ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt             >  2:315494/1‑101 (MQ=255)
                                                                                       cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgggcc            >  1:4288510/1‑99 (MQ=255)
                                                                                        gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           >  2:3192324/1‑101 (MQ=255)
                                                                                        gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           <  2:2817531/101‑1 (MQ=255)
                                                                                         aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          >  2:1614620/1‑101 (MQ=255)
                                                                                            tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct       >  1:105566/1‑101 (MQ=255)
                                                                                             aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc      <  1:4053625/101‑1 (MQ=255)
                                                                                             aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc      <  1:1463541/101‑1 (MQ=255)
                                                                                              tttGGAGCAAGATG.AAAGCCCATGGGTGCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACAGGGGGCAGGGCCCCCCTCCCGGGTCTTTCTCa     <  2:2850993/101‑1 (MQ=25)
                                                                                              tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa     >  1:2565598/1‑101 (MQ=255)
                                                                                              tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa     >  2:386828/1‑101 (MQ=255)
                                                                                                tGGATCTAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg   <  1:726063/101‑1 (MQ=255)
                                                                                                 ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  1:587326/1‑101 (MQ=255)
                                                                                                   |                                                                                                   
GCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640094‑2640291

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG