Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,198 A→G 24.3% intergenic (‑174/+102) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1980A→G24.3% 86.3 ‑0.6 ‑0.0136intergenic (‑174/+102)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/14):  ref base (52/51):  total (71/65)
Fisher's exact test strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.58e-01

Only 100 of 144 total aligned reads displayed.

GGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640099‑2640297
                                                                                                     |                                                                                                   
  gaGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                                                                   <  2:2587362/100‑1 (MQ=25)
  cAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                                                                   <  1:146990/101‑1 (MQ=37)
    ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                                 <  1:3280783/101‑1 (MQ=25)
     gcggtgccaCCGGGGCCGTTTTGCGCCAGCAAAAGGGGGGCCCCGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                                <  1:2615763/93‑1 (MQ=12)
       gtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                                                              <  2:2107149/101‑3 (MQ=25)
          tCAACCGTGGCGCGTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCa                                                                                          <  1:155103/101‑1 (MQ=21)
            aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                        <  1:94532/101‑1 (MQ=25)
                  ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                                                                  <  1:3877557/101‑1 (MQ=25)
                       ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTTGC.CTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATGAAAA.ACCATGGGTTCATCTTg                                                                             >  2:4264698/1‑101 (MQ=21)
                       ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                             <  2:3070734/101‑1 (MQ=21)
                       ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                             <  2:3821418/101‑1 (MQ=25)
                              gccccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                                     >  2:381633/7‑101 (MQ=14)
                               ccccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAata                                                                    <  2:1672072/96‑1 (MQ=17)
                                cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                                   <  1:1435728/97‑1 (MQ=17)
                                 ccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                                                                  >  2:3812970/4‑101 (MQ=17)
                                   gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                <  2:2584643/100‑1 (MQ=17)
                                   gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                <  2:1214460/100‑1 (MQ=17)
                                   gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                >  2:2237596/2‑101 (MQ=17)
                                   aaaaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATcc                                                                 <  1:1864880/101‑1 (MQ=21)
                                    aaaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACGCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                                <  2:2958783/101‑1 (MQ=25)
                                    aaaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                                <  2:3542036/101‑1 (MQ=21)
                                    aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               >  1:501348/1‑101 (MQ=17)
                                    aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               <  2:4434161/101‑1 (MQ=17)
                                    aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               >  1:284981/1‑101 (MQ=17)
                                    aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               >  1:3317379/1‑101 (MQ=17)
                                     aaagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                              >  2:1754790/4‑101 (MQ=17)
                                     aaagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                              >  2:2633283/4‑101 (MQ=17)
                                     aaagggagCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                              <  1:151104/98‑1 (MQ=18)
                                     aaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCg                                                               <  2:3915414/101‑1 (MQ=25)
                                      aagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                             >  2:3995351/3‑101 (MQ=18)
                                      aagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                             <  1:3925122/99‑1 (MQ=18)
                                       ggggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATGAAAA.ACCATGGGGTCATCTTTCCTCAAATATACCggg                                                             >  2:1520875/1‑101 (MQ=18)
                                        gggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                           >  1:2364239/1‑101 (MQ=18)
                                          gggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                         >  1:1483656/1‑99 (MQ=18)
                                          gggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATACCgggggg                                                          >  2:3952654/1‑101 (MQ=21)
                                           ggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                        <  2:2365971/101‑4 (MQ=21)
                                           ggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                        <  1:2684185/101‑4 (MQ=21)
                                            gcgcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                       <  1:371556/101‑5 (MQ=21)
                                            gcgcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGACATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                       >  2:170973/1‑97 (MQ=21)
                                              gcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGGGTc                                                      >  1:2493273/1‑101 (MQ=25)
                                               gctgcccccTC.TTGGCCTTTGGCCAATTC.CCCCCTGGGATATTTCGGGCAAGATGAAATCA.TGCCCCTTCATCTTGCCGCAAATATCCCGGGGGGAgcgaa                                                    <  2:519174/97‑5 (MQ=2)
                                               cTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                                    >  1:157205/1‑94 (MQ=25)
                                                 gCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggggaggcgaa                                                  >  2:60573/1‑93 (MQ=21)
                                                 gCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                   <  1:1819846/101‑1 (MQ=37)
                                                  ccccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                                                  <  2:19556/101‑1 (MQ=37)
                                                   cccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                 <  2:1107672/101‑1 (MQ=37)
                                                    ccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                                >  2:331491/1‑101 (MQ=37)
                                                      ccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                              >  2:1598174/1‑101 (MQ=37)
                                                      ccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                              <  1:978469/101‑1 (MQ=37)
                                                      ccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAga                                              >  1:3142495/1‑100 (MQ=37)
                                                        tccgatctcTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                             <  2:1831815/93‑1 (MQ=25)
                                                           ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                          >  1:196128/6‑101 (MQ=25)
                                                           ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                          >  2:3963879/6‑101 (MQ=37)
                                                             ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                        >  2:748150/4‑101 (MQ=37)
                                                              gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                       <  1:3135966/99‑1 (MQ=37)
                                                              gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                       >  1:208038/3‑101 (MQ=37)
                                                               ccTTCCGCCAATTCCCCCCCCGAGGTATTTGG.ACAAGGATAAAGGTCGAGGGGTTCACCTTGCTTAAAATATCCGGGGGGGAGTCCTGAAAGGACgggggg                                      <  1:2908755/100‑1 (MQ=255)
                                                                cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                     >  1:1412095/1‑101 (MQ=37)
                                                                  tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                   <  2:1389928/101‑1 (MQ=255)
                                                                    ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                 >  1:1566253/1‑101 (MQ=255)
                                                                    ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                 >  1:4394021/1‑101 (MQ=37)
                                                                    ggCCAACTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                 <  1:2543800/101‑1 (MQ=37)
                                                                     gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                <  2:863949/101‑1 (MQ=255)
                                                                       cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAANGACGGGGGGCAGAGccc                              >  2:1005714/1‑101 (MQ=37)
                                                                       cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                              >  2:4261646/1‑101 (MQ=37)
                                                                        aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                             <  1:1121558/101‑1 (MQ=255)
                                                                        aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                             >  2:4430714/1‑101 (MQ=37)
                                                                         aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCAAGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                            <  1:3478645/101‑1 (MQ=37)
                                                                          ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                           >  1:1268778/1‑101 (MQ=255)
                                                                          ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                           >  2:200119/1‑101 (MQ=255)
                                                                          ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                           >  1:4186309/1‑101 (MQ=255)
                                                                           tcaccccccGAGGTATTTGGCGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                          >  2:335974/4‑101 (MQ=25)
                                                                            caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                         <  2:4048920/99‑1 (MQ=255)
                                                                            caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                         >  2:2603201/3‑101 (MQ=255)
                                                                            caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCACCCTc                         >  2:3365057/3‑101 (MQ=37)
                                                                              ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                       <  2:2476156/101‑1 (MQ=255)
                                                                              ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                       >  1:1780964/1‑101 (MQ=255)
                                                                               cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                      <  1:2603372/101‑1 (MQ=255)
                                                                               cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                      <  1:1598280/101‑1 (MQ=255)
                                                                                  ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                   >  2:315494/1‑101 (MQ=255)
                                                                                  ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                   >  2:3411138/1‑101 (MQ=255)
                                                                                   cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgggcc                  >  1:4288510/1‑99 (MQ=255)
                                                                                    gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                 >  2:3192324/1‑101 (MQ=255)
                                                                                    gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                 <  2:2817531/101‑1 (MQ=255)
                                                                                     aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                <  2:2390866/101‑1 (MQ=255)
                                                                                       gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc              >  1:2914141/1‑101 (MQ=255)
                                                                                        tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             >  1:105566/1‑101 (MQ=255)
                                                                                         aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc            <  1:4053625/101‑1 (MQ=255)
                                                                                          tttGGAGCAAGATG.AAAGCCCATGGGTGCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACAGGGGGCAGGGCCCCCCTCCCGGGTCTTTCTCa           <  2:2850993/101‑1 (MQ=25)
                                                                                          tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           >  2:386828/1‑101 (MQ=255)
                                                                                          tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           >  1:2565598/1‑101 (MQ=255)
                                                                                            tGGATCTAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg         <  1:726063/101‑1 (MQ=255)
                                                                                             ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc        >  1:587326/1‑101 (MQ=255)
                                                                                              gagcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg       >  2:1319283/4‑101 (MQ=255)
                                                                                                 cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc    >  2:142278/1‑101 (MQ=255)
                                                                                                 cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc    >  1:3433657/1‑101 (MQ=255)
                                                                                                  aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc   >  2:1765027/1‑101 (MQ=255)
                                                                                                   aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  >  2:1016187/1‑101 (MQ=255)
                                                                                                   aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  >  2:4369361/1‑101 (MQ=255)
                                                                                                   aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  <  1:2729410/101‑1 (MQ=255)
                                                                                                     |                                                                                                   
GGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640099‑2640297

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG