Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,210 T→G 23.4% intergenic (‑186/+90) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2100T→G23.4% 99.8 ‑0.2 ‑0.1144intergenic (‑186/+90)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/14):  ref base (65/44):  total (84/60)
Fisher's exact test strand distribution p-value = 8.42e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.43e-01

Only 100 of 151 total aligned reads displayed.

AACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGA  >  NC_014034/2640111‑2640310
                                                                                                     |                                                                                                    
aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                                     <  1:94532/101‑1 (MQ=25)
  ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                                                   <  2:3936702/101‑1 (MQ=25)
      ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                                                                               <  1:3877557/101‑1 (MQ=25)
         gcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCCTCGGCCAATTCACCCCCCCGGGGATTTGGAGGAAGATG.AAAGAACAAGGGTTCATcc                                                                                            >  1:1390025/1‑100 (MQ=16)
           ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTTGC.CTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATGAAAA.ACCATGGGTTCATCTTg                                                                                          >  2:4264698/1‑101 (MQ=21)
           ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                                          <  2:3821418/101‑1 (MQ=25)
           ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                                          <  2:3070734/101‑1 (MQ=21)
               gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                                      <  2:2245420/101‑1 (MQ=25)
                   ccccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAata                                                                                 <  2:1672072/96‑1 (MQ=17)
                    cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                                                <  1:1435728/97‑1 (MQ=17)
                     ccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                                                                               >  2:3812970/4‑101 (MQ=17)
                      cgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                                              >  1:2516379/3‑101 (MQ=17)
                       gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                             <  2:2584643/100‑1 (MQ=17)
                       gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                             >  2:2237596/2‑101 (MQ=17)
                       gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                             >  1:3221725/2‑101 (MQ=17)
                       aaaaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATcc                                                                              <  1:1864880/101‑1 (MQ=21)
                        aaaGGGGGGCGCTGCCGCCCTC.TTGGCCTTCGGTCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                                             <  2:2034580/101‑1 (MQ=25)
                        aaaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                                             <  2:3542036/101‑1 (MQ=21)
                        aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                            <  2:4434161/101‑1 (MQ=17)
                        aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                            >  1:3317379/1‑101 (MQ=17)
                        aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                            >  1:284981/1‑101 (MQ=17)
                         aaagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                                           >  2:2633283/4‑101 (MQ=17)
                         aaagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                                           >  2:1754790/4‑101 (MQ=17)
                         aaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCg                                                                            <  2:3915414/101‑1 (MQ=25)
                           ggggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                                                                          <  1:3064119/101‑1 (MQ=25)
                           ggggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATGAAAA.ACCATGGGGTCATCTTTCCTCAAATATACCggg                                                                          >  2:1520875/1‑101 (MQ=18)
                            gggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                                        >  1:2364239/1‑101 (MQ=18)
                            gggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                                                                        <  2:2442551/101‑1 (MQ=18)
                             ggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                                                       <  2:4170680/101‑2 (MQ=18)
                              gggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                                      >  1:1483656/1‑99 (MQ=18)
                              gggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                                       <  1:3798836/101‑1 (MQ=25)
                               ggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                                     <  1:2684185/101‑4 (MQ=21)
                                gcgcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                                    <  2:1301882/101‑5 (MQ=21)
                                gcgcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTTTTTGGAGCAAGATG.AAAGAACATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg                                                                     >  1:1491290/1‑101 (MQ=25)
                                  gcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                                                  >  2:2897688/1‑95 (MQ=21)
                                   gctgcccccTC.TTGGCCTTTGGCCAATTC.CCCCCTGGGATATTTCGGGCAAGATGAAATCA.TGCCCCTTCATCTTGCCGCAAATATCCCGGGGGGAgcgaa                                                                 <  2:519174/97‑5 (MQ=2)
                                   cTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                                                 >  1:157205/1‑94 (MQ=25)
                                   cTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                                  >  2:258998/1‑101 (MQ=25)
                                   cTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                                  >  2:1747285/1‑101 (MQ=25)
                                     gCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggggaggcgaa                                                               >  2:60573/1‑93 (MQ=21)
                                     gCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                                <  1:1819846/101‑1 (MQ=37)
                                       tctccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                              <  2:3142271/98‑1 (MQ=37)
                                       cccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                              <  2:1107672/101‑1 (MQ=37)
                                        ccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                                             >  2:331491/1‑101 (MQ=37)
                                          ccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                           <  1:978469/101‑1 (MQ=37)
                                          ccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                           >  2:192453/1‑101 (MQ=37)
                                          ccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                           <  1:2747996/101‑1 (MQ=37)
                                            tccgatctcTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                                          <  2:1831815/93‑1 (MQ=25)
                                               ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                                       >  1:196128/6‑101 (MQ=25)
                                               ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                                       >  2:3963879/6‑101 (MQ=37)
                                                tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                      >  2:2729220/5‑101 (MQ=37)
                                                 ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGCGGGAGTCCTGAAAGGACgggg                                                     >  2:3965748/4‑101 (MQ=37)
                                                  gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                                    <  1:3135966/99‑1 (MQ=37)
                                                  gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                                    >  1:208038/3‑101 (MQ=37)
                                                    cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                                  >  1:1412095/1‑101 (MQ=37)
                                                        ggCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                              <  2:3832394/101‑1 (MQ=255)
                                                        ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                              >  1:1566253/1‑101 (MQ=255)
                                                        ggCCAACTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                              <  1:2543800/101‑1 (MQ=37)
                                                         gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                             <  2:863949/101‑1 (MQ=255)
                                                           cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAANGACGGGGGGCAGAGccc                                           >  2:1005714/1‑101 (MQ=37)
                                                           cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                                           >  2:106276/1‑101 (MQ=37)
                                                            aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                          >  2:4430714/1‑101 (MQ=37)
                                                             aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCAAGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                         <  1:3478645/101‑1 (MQ=37)
                                                              ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                        >  1:1268778/1‑101 (MQ=255)
                                                              ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                        >  2:200119/1‑101 (MQ=255)
                                                              ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                        >  1:4186309/1‑101 (MQ=255)
                                                               tcaccccccGAGGTATTTGGCGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                       >  2:335974/4‑101 (MQ=25)
                                                                caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                      <  2:4048920/99‑1 (MQ=255)
                                                                caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCACCCTc                                      >  2:3365057/3‑101 (MQ=37)
                                                                 cccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                     <  1:4264967/100‑1 (MQ=255)
                                                                  ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                                    >  1:1780964/1‑101 (MQ=255)
                                                                  ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                                    <  1:260154/101‑1 (MQ=255)
                                                                   cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                                   <  1:1598280/101‑1 (MQ=255)
                                                                   cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                                   <  1:2603372/101‑1 (MQ=255)
                                                                     cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg                                 <  1:458166/101‑1 (MQ=255)
                                                                      ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                                >  2:3411138/1‑101 (MQ=255)
                                                                       cGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgggcc                               >  1:4288510/1‑99 (MQ=255)
                                                                        gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                              >  2:3192324/1‑101 (MQ=255)
                                                                         aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                             >  2:1614620/1‑101 (MQ=255)
                                                                         aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                             <  2:2390866/101‑1 (MQ=255)
                                                                           gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc                           >  1:2914141/1‑101 (MQ=255)
                                                                            tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                          >  1:105566/1‑101 (MQ=255)
                                                                              tttGGAGCAAGATG.AAAGCCCATGGGTGCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACAGGGGGCAGGGCCCCCCTCCCGGGTCTTTCTCa                        <  2:2850993/101‑1 (MQ=25)
                                                                                tGGATCTAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg                      <  1:726063/101‑1 (MQ=255)
                                                                                 ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                     >  1:587326/1‑101 (MQ=255)
                                                                                  gagcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg                    >  2:1319283/4‑101 (MQ=255)
                                                                                    gcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGtt                  <  1:3952908/100‑1 (MQ=255)
                                                                                     cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                 >  1:3433657/1‑101 (MQ=255)
                                                                                     cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                 >  2:142278/1‑101 (MQ=255)
                                                                                      aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc                >  2:3736712/1‑101 (MQ=255)
                                                                                       aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg               >  2:4369361/1‑101 (MQ=255)
                                                                                         gatgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCgg              >  1:3611574/5‑101 (MQ=255)
                                                                                            gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc           >  1:1523/2‑101 (MQ=255)
                                                                                            gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc           >  1:2707711/2‑101 (MQ=255)
                                                                                             aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa          >  1:3678851/1‑101 (MQ=255)
                                                                                              aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg         <  1:4430986/101‑1 (MQ=255)
                                                                                              aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg         >  1:3996854/1‑101 (MQ=255)
                                                                                                 aCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGacc      >  1:1059562/1‑99 (MQ=255)
                                                                                                   cATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCg    >  1:4132282/1‑101 (MQ=255)
                                                                                                    aTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCgg   >  1:2942608/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
AACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGA  >  NC_014034/2640111‑2640310

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG