Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,225 T→C 20.2% intergenic (‑201/+75) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2250T→C20.2% 86.8 ‑1.2 ‑0.0164intergenic (‑201/+75)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/14):  ref base (88/42):  total (107/57)
Fisher's exact test strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.94e-01

Only 100 of 172 total aligned reads displayed.

GCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCGCGCGCACCT  >  NC_014034/2640126‑2640325
                                                                                                    |                                                                                                    
gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                                                     <  2:2245420/101‑1 (MQ=25)
   gccccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                                                                 >  2:381633/7‑101 (MQ=14)
     cccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                                                               <  1:1435728/97‑1 (MQ=17)
      ccgaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                                                                                              >  2:3812970/4‑101 (MQ=17)
        gaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                                            <  2:2584643/100‑1 (MQ=17)
        gaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                                            <  2:1214460/100‑1 (MQ=17)
        gaaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                                            >  1:983552/2‑101 (MQ=17)
         aaaGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCCATTCACCCCCCGAGGGATTTGGGGCAAGATG.AAAGACCATAGGGTCATCTTGCCTCAAATATccc                                                                                            >  2:4382118/1‑101 (MQ=18)
         aaaGGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                                                            <  2:3542036/101‑1 (MQ=21)
         aaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                                           >  1:284981/1‑101 (MQ=17)
         aaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                                           <  2:4434161/101‑1 (MQ=17)
         aaaAGGGGGCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                                           >  1:3317379/1‑101 (MQ=17)
          aaagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                                                          >  2:2633283/4‑101 (MQ=17)
          aaagggagCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                                                          <  1:151104/98‑1 (MQ=18)
           aagggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                                                                                         <  1:3925122/99‑1 (MQ=18)
            ggggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATGAAAA.ACCATGGGGTCATCTTTCCTCAAATATACCggg                                                                                         >  2:1520875/1‑101 (MQ=18)
              ggggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                                                                      <  2:4170680/101‑2 (MQ=18)
               gggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                                                     >  1:1483656/1‑99 (MQ=18)
               gggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATACCgggggg                                                                                      >  2:3952654/1‑101 (MQ=21)
                ggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                                                    <  1:2684185/101‑4 (MQ=21)
                ggCGCTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                                                     <  1:1569019/101‑1 (MQ=25)
                 gcgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                                                   <  1:371556/101‑5 (MQ=21)
                 gcgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGACATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                                                   >  2:170973/1‑97 (MQ=21)
                  cgcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                                                  <  2:3386072/101‑6 (MQ=21)
                   gcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                                                                 >  2:2897688/1‑95 (MQ=21)
                   gcTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGGGTc                                                                                  >  1:2493273/1‑101 (MQ=25)
                    cTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                                                 >  2:258998/1‑101 (MQ=25)
                    cTGCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                                                 >  2:1747285/1‑101 (MQ=25)
                      gCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggggaggcgaa                                                                              >  2:60573/1‑93 (MQ=21)
                      gCCCCCCTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                                               <  1:1819846/101‑1 (MQ=37)
                       ccccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                                                                              <  2:19556/101‑1 (MQ=37)
                        tctccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                                             <  2:3142271/98‑1 (MQ=37)
                        cccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                                             <  2:1107672/101‑1 (MQ=37)
                         ccccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                                                            >  2:331491/1‑101 (MQ=37)
                           ccTCTTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                                          <  1:2747996/101‑1 (MQ=37)
                             tctTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                                        >  2:458126/1‑101 (MQ=37)
                               ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                                                      >  1:196128/6‑101 (MQ=25)
                               ttggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgg                                                                      >  2:3963879/6‑101 (MQ=37)
                                 ggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGCGGGAGTCCTGAAAGGACgggg                                                                    >  2:3965748/4‑101 (MQ=37)
                                  gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                                                   <  1:3135966/99‑1 (MQ=37)
                                  gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                                                   >  1:208038/3‑101 (MQ=37)
                                    cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGaagatcgga                                                                 >  1:1831939/1‑93 (MQ=37)
                                        ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                                             >  1:4394021/1‑101 (MQ=37)
                                        ggCCAACTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                                             <  1:2543800/101‑1 (MQ=37)
                                         gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                                            <  2:863949/101‑1 (MQ=255)
                                           cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAANGACGGGGGGCAGAGccc                                                          >  2:1005714/1‑101 (MQ=37)
                                           cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                                                          >  2:106276/1‑101 (MQ=37)
                                            aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                                         <  1:1121558/101‑1 (MQ=255)
                                              ttCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                                       <  1:830262/101‑1 (MQ=255)
                                              ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                                       >  1:1268778/1‑101 (MQ=255)
                                              ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                                       >  2:200119/1‑101 (MQ=255)
                                              ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                                       >  1:4186309/1‑101 (MQ=255)
                                               tcaccccccGAGGTATTTGGCGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                                      >  2:335974/4‑101 (MQ=25)
                                                  ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                                                   <  2:2476156/101‑1 (MQ=255)
                                                  ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                                                   <  1:260154/101‑1 (MQ=255)
                                                   cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                                                  <  1:2603372/101‑1 (MQ=255)
                                                   cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                                                  <  1:1598280/101‑1 (MQ=255)
                                                     cccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCggg                                                <  1:458166/101‑1 (MQ=255)
                                                        gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                                             >  2:3192324/1‑101 (MQ=255)
                                                        gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                                             <  2:2817531/101‑1 (MQ=255)
                                                         aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                                            >  2:1614620/1‑101 (MQ=255)
                                                         aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                                            <  2:2390866/101‑1 (MQ=255)
                                                           gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc                                          >  1:2914141/1‑101 (MQ=255)
                                                            tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                                         >  1:105566/1‑101 (MQ=255)
                                                             aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                                        <  1:4053625/101‑1 (MQ=255)
                                                              tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa                                       >  2:386828/1‑101 (MQ=255)
                                                                 ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                                    >  1:587326/1‑101 (MQ=255)
                                                                  gagcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg                                   >  2:1319283/4‑101 (MQ=255)
                                                                    gcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGtt                                 <  1:3952908/100‑1 (MQ=255)
                                                                     cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                                >  1:3433657/1‑101 (MQ=255)
                                                                     cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                                >  2:142278/1‑101 (MQ=255)
                                                                      aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc                               >  2:3736712/1‑101 (MQ=255)
                                                                      aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc                               >  2:1765027/1‑101 (MQ=255)
                                                                            gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc                          >  1:1523/2‑101 (MQ=255)
                                                                             aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa                         >  1:3678851/1‑101 (MQ=255)
                                                                               aGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGa                       >  1:3573452/1‑101 (MQ=255)
                                                                                gACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa                      >  2:2105218/1‑101 (MQ=255)
                                                                                 aCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGacc                     >  1:1059562/1‑99 (MQ=255)
                                                                                  ccATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAcc                    >  1:4257064/1‑101 (MQ=255)
                                                                                    aTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCgg                  >  1:1410216/1‑101 (MQ=255)
                                                                                     tGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCCGAACCGGa                 >  1:3034904/1‑101 (MQ=255)
                                                                                       ggTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAg               >  2:4272511/1‑101 (MQ=255)
                                                                                       ggTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAg               >  1:2337729/1‑101 (MQ=255)
                                                                                        gTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGc              >  1:3515356/1‑101 (MQ=255)
                                                                                          tCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGccc            >  2:4020452/1‑101 (MQ=255)
                                                                                          tCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGccc            >  2:286577/1‑101 (MQ=255)
                                                                                          tCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGccc            >  1:4283335/1‑101 (MQ=255)
                                                                                           cATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcg           >  1:328810/1‑101 (MQ=255)
                                                                                           cATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcg           >  2:1167542/1‑101 (MQ=255)
                                                                                            aTCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcgc          >  2:346754/1‑101 (MQ=255)
                                                                                            aTCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcgc          >  1:2726166/1‑101 (MQ=255)
                                                                                             tCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcgcg         >  2:4050342/1‑101 (MQ=255)
                                                                                              tttGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGACCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcgcgc        <  1:315529/100‑1 (MQ=255)
                                                                                               ttGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcgcgcg       >  2:1899747/1‑101 (MQ=255)
                                                                                                 gCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCGCGCGCa     >  1:3046415/1‑101 (MQ=255)
                                                                                                 gCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCGCGCGCa     >  1:490785/1‑101 (MQ=255)
                                                                                                  ccTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCGCGCGCAc    <  1:1771486/101‑1 (MQ=255)
                                                                                                   cTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCGCGCGCAcc   >  1:170820/1‑101 (MQ=255)
                                                                                                   cTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCGCGCGCAcc   >  2:2307079/1‑101 (MQ=255)
                                                                                                    tCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCGCGCGCACCt  >  1:1605835/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCGCGCGCACCT  >  NC_014034/2640126‑2640325

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG